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PangenomeTIGR4
serotype 4
D39
serotype 2
D39V
serotype 2
Hungary19A-6
serotype 19A
EF3030
serotype 19F
670-6B
serotype 6B
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serotype 6A
70585
serotype 5
A026
serotype 19F
A66
serotype 3
AP200
serotype 11A
ASP0581
serotype 12F
ATCC 49619
serotype 19F
ATCC 700669
serotype 23F
BVJ1JL
serotype 1
CGSP14
serotype 14
G54
serotype 19F
HU-OH
serotype 3
Hu15
serotype 19A
Hu17
serotype 19A
INV104
serotype 1
INV200
serotype 14
JJA
serotype 14
MDRSPN001
serotype 19F
NCTC7466
serotype 2
NU83127
serotype 4
OXC141
serotype 3
P1031
serotype 1
R6
serotype 2
SP49
serotype 19A
SPN032672
serotype 1
SPN034156
serotype 3
SPN034183
serotype 3
SPN994038
serotype 3
SPN994039
serotype 3
SPNA45
serotype 3
ST556
serotype 19F
TCH8431/19A
serotype 19A
Taiwan19F-14
serotype 19F
Xen35
serotype 4
gamPNI0373
serotype 1

NCBI: 02-MAY-2018

⊟Summary[edit | edit source]

  • organism: Streptococcus pneumoniae D39
  • locus tag: SPD_1497 [new locus tag: SPD_RS07955 ]
  • pan locus tag?: PNEUPAN003082000
  • symbol: nanE-1
  • pan gene symbol?: nanE-1
  • synonym:
  • product: N-acetylmannosamine-6-phosphate 2-epimerase 2, putative

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SPD_1497 [new locus tag: SPD_RS07955 ]
  • symbol: nanE-1
  • product: N-acetylmannosamine-6-phosphate 2-epimerase 2, putative
  • replicon: chromosome
  • strand: -
  • coordinates: 1513753..1514451
  • length: 699
  • essential: unknown

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    ATGCCACAGATTAGTAAAGAAGCCTTGATTGAGCAAATCAAAGATGGAATCATCGTTTCT
    TGTCAGGCTCTTCCTCATGAACCGCTTTATACAGAAGCGGGAGGGGTGATTCCCTTGCTG
    GTCAAAGCGGCTGAGCAAGGTGGAGCAGTCGGTATCCGAGCAAACAGTGTTCGCGATATC
    AAGGAAATTAAGGAAGTCACTAAACTTCCAATCATTGGGATTATCAAACGTGATTATCCA
    CCTCAGGAACCCTTCATCACGGCTACTATGAAAGAAGTTGATGAATTGGCAGAACTGGAC
    ATCGAGGTGATTGCTCTGGATTGTACCAAGCGTGAACGCTACGATGGTTTGGAAATTCAA
    GAGTTCATTCGTCAGGTTAAGGAGAAATATCCTAATCAGCTTTTGATGGCTGATACTAGT
    ATCTTCGAAGAAGGGCTAGCAGCTGTAGAAGCAGGAATTGACTTTGTCGGAACAACCTTA
    TCAGGCTACACATCCTACAGTCCAAAAGTAGACGGTCCAGATTTTGAATTGATTAAGAAA
    CTCTGTGATGCCGGTGTAGATGTCATTGCAGAAGGAAAAATTCATACACCAGAACAAGCC
    AAACAAATCCTTGAATATGGAGTGCGAGGCATCGTTGTTGGTGGCGCCATTACTAGACCA
    AAAGAGATTACAGAACGCTTCGTTGCTAGTCTTAAATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    699

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SPD_1497 [new locus tag: SPD_RS07955 ]
  • symbol: NanE-1
  • description: N-acetylmannosamine-6-phosphate 2-epimerase 2, putative
  • length: 232
  • theoretical pI: 4.55017
  • theoretical MW: 25450.2
  • GRAVY: -0.0633621

⊟Function[edit | edit source]

  • reaction:
    EC 5.1.3.9?  ExPASy
    N-acylglucosamine-6-phosphate 2-epimerase N-acyl-D-glucosamine 6-phosphate = N-acyl-D-mannosamine 6-phosphate
  • TIGRFAM:
    Metabolism Amino acid biosynthesis Histidine family 1-(5-phosphoribosyl)-5-[(5-phosphoribosylamino)methylideneamino]imidazole-4-carboxamide isomerase (TIGR00007; EC 5.3.1.16; HMM-score: 19.5)
    Metabolism Amino acid biosynthesis Aromatic amino acid family tryptophan synthase, alpha subunit (TIGR00262; EC 4.2.1.20; HMM-score: 18.5)
    Unknown function General putative TIM-barrel protein, nifR3 family (TIGR00737; HMM-score: 17.5)
    3-hexulose-6-phosphate synthase (TIGR03128; EC 4.1.2.43; HMM-score: 17.5)
    and 6 more
    Metabolism Energy metabolism Other deoxyribose-phosphate aldolase (TIGR00126; EC 4.1.2.4; HMM-score: 14.1)
    Metabolism Purines, pyrimidines, nucleosides, and nucleotides Other deoxyribose-phosphate aldolase (TIGR00126; EC 4.1.2.4; HMM-score: 14.1)
    Unknown function General hisA/hisF family protein (TIGR00734; HMM-score: 13.9)
    Metabolism Purines, pyrimidines, nucleosides, and nucleotides Purine ribonucleotide biosynthesis inosine-5'-monophosphate dehydrogenase (TIGR01302; EC 1.1.1.205; HMM-score: 13.4)
    Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Thiamine thiamine-phosphate diphosphorylase (TIGR00693; EC 2.5.1.3; HMM-score: 12.1)
    mycofactocin system FadH/OYE family oxidoreductase 2 (TIGR03997; EC 1.-.-.-; HMM-score: 11.3)
  • TheSEED  :
    • N-acetylmannosamine-6-phosphate 2-epimerase (EC 5.1.3.9)
    Cell Wall and Capsule Capsular and extracellular polysacchrides Sialic Acid Metabolism  N-acetylmannosamine-6-phosphate 2-epimerase (EC 5.1.3.9)
  • PFAM:
    TIM_barrel (CL0036) NanE; Putative N-acetylmannosamine-6-phosphate epimerase (PF04131; HMM-score: 259.6)
    and 8 more
    IMPDH; IMP dehydrogenase / GMP reductase domain (PF00478; HMM-score: 18.6)
    Dus; Dihydrouridine synthase (Dus) (PF01207; HMM-score: 17.5)
    IGPS; Indole-3-glycerol phosphate synthase (PF00218; HMM-score: 15.3)
    His_biosynth; Histidine biosynthesis protein (PF00977; HMM-score: 14.5)
    ThiG; Thiazole biosynthesis protein ThiG (PF05690; HMM-score: 13.6)
    TMP-TENI; Thiamine monophosphate synthase (PF02581; HMM-score: 12)
    G3P_antiterm; Glycerol-3-phosphate responsive antiterminator (PF04309; HMM-score: 12)
    FMN_dh; FMN-dependent dehydrogenase (PF01070; HMM-score: 11.6)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.008727
    • TAT(Tat/SPI): 0.004406
    • LIPO(Sec/SPII): 0.021527
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MPQISKEALIEQIKDGIIVSCQALPHEPLYTEAGGVIPLLVKAAEQGGAVGIRANSVRDIKEIKEVTKLPIIGIIKRDYPPQEPFITATMKEVDELAELDIEVIALDCTKRERYDGLEIQEFIRQVKEKYPNQLLMADTSIFEEGLAAVEAGIDFVGTTLSGYTSYSPKVDGPDFELIKKLCDAGVDVIAEGKIHTPEQAKQILEYGVRGIVVGGAITRPKEITERFVASLK

⊟Experimental data[edit | edit source]

  • protein localization:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulators: NanR* (repression) regulon, CcpA regulon
    NanR*(TF)important in Sialic acid utilization; RegPrecise 
    CcpA(TF)important in Global catabolite repression; RegPrecise 

⊟Expression data[edit | edit source]

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

⊟Relevant publications[edit | edit source]

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