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serotype 4
D39
serotype 2
D39V
serotype 2
Hungary19A-6
serotype 19A
EF3030
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670-6B
serotype 6B
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serotype 6A
70585
serotype 5
A026
serotype 19F
A66
serotype 3
AP200
serotype 11A
ASP0581
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ATCC 49619
serotype 19F
ATCC 700669
serotype 23F
BM6001
serotype 19F
BVJ1JL
serotype 1
CGSP14
serotype 14
G54
serotype 19F
HU-OH
serotype 3
Hu15
serotype 19A
Hu17
serotype 19A
INV104
serotype 1
INV200
serotype 14
JJA
serotype 14
MDRSPN001
serotype 19F
NCTC7465
serotype 1
NCTC7466
serotype 2
NU83127
serotype 4
OXC141
serotype 3
P1031
serotype 1
R6
serotype 2
SP49
serotype 19A
SPN032672
serotype 1
SPN034156
serotype 3
SPN034183
serotype 3
SPN994038
serotype 3
SPN994039
serotype 3
SPNA45
serotype 3
ST556
serotype 19F
TCH8431/19A
serotype 19A
Taiwan19F-14
serotype 19F
Xen35
serotype 4
gamPNI0373
serotype 1

NCBI: 02-MAY-2018

⊟Summary[edit | edit source]

  • organism: Streptococcus pneumoniae D39
  • locus tag: SPD_2012 [new locus tag: SPD_RS10645 ]
  • pan locus tag?: PNEUPAN003881000
  • symbol: glpO
  • pan gene symbol?: glpO
  • synonym:
  • product: alpha-glycerophosphate oxidase

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SPD_2012 [new locus tag: SPD_RS10645 ]
  • symbol: glpO
  • product: alpha-glycerophosphate oxidase
  • replicon: chromosome
  • strand: -
  • coordinates: 1987849..1989675
  • length: 1827
  • essential: unknown

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    ATGGAATTTTCAAAAAAAACACGTGAATTGTCAATTAAAAAAATGCAGGAACGCACCCTG
    GACCTCTTGATTATCGGTGGAGGAATCACAGGAGCTGGTGTAGCCTTGCAGGCGGCAGCT
    AGCGGTCTTGAGACTGGTTTGATTGAAATGCAAGACTTTGCAGAAGGAACATCTAGTCGT
    TCAACAAAATTGGTTCACGGAGGTCTTCGCTACCTCAAACAATTCGACGTAGAAGTGGTC
    TCAGATACGGTTTCTGAACGTGCAGTGGTTCAACAAATCGCTCCACACATTCCAAAACCA
    GATCCAATGCTCTTACCAGTTTACGATGAAGATGGAGCAACCTTTAGCCTCTTCCGTCTT
    AAAGTAGCCATGGACTTGTACGACCTCTTGGCAGGTGTTAGCAACACACCAACTGCGAAC
    AAGGTTTTGAGCAAGGATCAAGTCTTGGAACGCCAGCCAAACTTGAAGAAGGAAGGCTTG
    GTAGGAGGTGGAGTGTATCTTGACTTCCGTAACAACGATGCGCGTCTCGTGATTGAAAAC
    ATCAAACGTGCCAACCAAGACGGTGCCCTCATTGCCAACCACGTGAAGGCAGAAGGCTTC
    CTCTTTGACGAAAGTGGCAAGATTACAGGTGTTGTAGCTCGTGATCTCTTGACAGACCAA
    GTATTTGAAATCAAGGCCCGTCTGGTTATTAATACAACAGGTCCTTGGAGTGATAAAGTA
    CGTAATTTGTCTAATAAGGGAACGCAATTCTCACAAATGCGCCCAACTAAGGGAGTTCAC
    TTGGTAGTGGATTCAAGCAAAATCAAGGTTTCACAGCCAGTTTACTTTGACACAGGTTTG
    GGTGACGGTCGTATGGTCTTTGTTCTCCCACGTGAAAACAAGACTTACTTTGGTACAACT
    GATACAGACTACACAGGTGATTTGGAGCATCCAAAAGTAACTCAAGAAGATGTAGATTAT
    CTACTTGGCATTGTCAACAACCGCTTTCCAGAATCCAACATCACCATTGATGATATCGAA
    AGCAGCTGGGCAGGTCTTCGTCCATTGATTGCAGGGAACAGTGCCTCTGACTATAATGGT
    GGAAATAACGGTACCATCAGTGATGAAAGCTTTGACAACTTGATTGCGACTGTTGAATCT
    TATCTCTCCAAAGAAAAAACACGTGAAGATGTTGAGTCTGCTGTCAGCAAGCTTGAAAGT
    AGCACATCTGAGAAACATTTGGATCCATCTGCAGTTTCTCGTGGGTCTAGCTTGGATCGT
    GATGACAATGGTCTTTTGACTCTTGCTGGCGGTAAAATCACAGACTACCGTAAGATGGCT
    GAAGGAGCTATGGAGCGCGTGGTTGACATCCTCAAAGCAGAATTTGACCGTAGCTTTAAA
    TTGATCAATTCTAAAACTTACCCTGTTTCAGGTGGAGAATTGAACCCAGCAAATGTGGAT
    TCAGAAATCGAAGCCTTTGCGCAACTTGGAGTTTCACGTGGTTTGGATAGCAAGGAAGCT
    CATTACCTAGCAAATCTTTACGGTTCAAATGCACCGAAGGTCTTTGCACTTGCTCACAGC
    TTGGAACAAGCGCCAGGACTCAGCTTGGCAGATACTTTGTCCCTTCACTATGCAATGCGC
    AACGAGTTGGCTCTTAGCCCAGTTGACTTCCTTCTTCGTCGTACCAACCATATGCTCTTT
    ATGCGTGATAGCTTGGATAGCATCGTTGAGCCAGTTTTGGATGAAATGGGACGATTCTAT
    GACTGGACAGAAGAAGAAAAAGCAACTTACCGTGCTGATGTCGAAGCAGCTCTCGCTAAC
    AACGATTTAGCAGAATTAAAAAATTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1320
    1380
    1440
    1500
    1560
    1620
    1680
    1740
    1800
    1827

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SPD_2012 [new locus tag: SPD_RS10645 ]
  • symbol: GlpO
  • description: alpha-glycerophosphate oxidase
  • length: 608
  • theoretical pI: 4.73902
  • theoretical MW: 66791.7
  • GRAVY: -0.322204

⊟Function[edit | edit source]

  • reaction:
    EC 1.1.3.21?  ExPASy
    Glycerol-3-phosphate oxidase sn-glycerol 3-phosphate + O2 = glycerone phosphate + H2O2
  • TIGRFAM:
    Metabolism Energy metabolism Anaerobic glycerol-3-phosphate dehydrogenase, anaerobic, A subunit (TIGR03377; EC 1.1.5.3; HMM-score: 144.8)
    and 17 more
    Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Thiamine glycine oxidase ThiO (TIGR02352; EC 1.4.3.19; HMM-score: 31.9)
    Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Chlorophyll and bacteriochlorphyll geranylgeranyl reductase family (TIGR02032; EC 1.3.1.-; HMM-score: 21.4)
    FAD dependent oxidoreductase TIGR03364 (TIGR03364; HMM-score: 21.3)
    Genetic information processing Protein synthesis tRNA and rRNA base modification tRNA U-34 5-methylaminomethyl-2-thiouridine biosynthesis protein MnmC, C-terminal domain (TIGR03197; HMM-score: 17.7)
    Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Menaquinone and ubiquinone ubiquinone biosynthesis hydroxylase, UbiH/UbiF/VisC/COQ6 family (TIGR01988; EC 1.14.13.-; HMM-score: 17.3)
    Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Menaquinone and ubiquinone 2-polyprenyl-6-methoxyphenol 4-hydroxylase (TIGR01984; EC 1.14.13.-; HMM-score: 17.1)
    lycopene cyclase family protein (TIGR01790; HMM-score: 14.5)
    Cellular processes Cellular processes Detoxification alkyl hydroperoxide reductase subunit F (TIGR03140; EC 1.8.1.-; HMM-score: 13.7)
    Cellular processes Cellular processes Adaptations to atypical conditions alkyl hydroperoxide reductase subunit F (TIGR03140; EC 1.8.1.-; HMM-score: 13.7)
    Metabolism Energy metabolism Electron transport flavocytochrome c (TIGR01813; HMM-score: 12.5)
    dihydrolipoyl dehydrogenase (TIGR01350; EC 1.8.1.4; HMM-score: 12)
    Metabolism Energy metabolism Amino acids and amines sarcosine oxidase, alpha subunit family (TIGR01372; HMM-score: 11.8)
    Metabolism Energy metabolism Anaerobic glycerol-3-phosphate dehydrogenase, anaerobic, B subunit (TIGR03378; EC 1.1.5.3; HMM-score: 11.8)
    Metabolism Energy metabolism TCA cycle succinate dehydrogenase or fumarate reductase, flavoprotein subunit (TIGR01812; HMM-score: 11.3)
    Metabolism Energy metabolism Anaerobic succinate dehydrogenase or fumarate reductase, flavoprotein subunit (TIGR01812; HMM-score: 11.3)
    Metabolism Energy metabolism Aerobic succinate dehydrogenase or fumarate reductase, flavoprotein subunit (TIGR01812; HMM-score: 11.3)
    squalene-associated FAD-dependent desaturase (TIGR03467; HMM-score: 10.4)
  • TheSEED  :
    • Alpha-glycerophosphate oxidase (EC 1.1.3.21)
    Carbohydrates Sugar alcohols Glycerol and Glycerol-3-phosphate Uptake and Utilization  Alpha-glycerophosphate oxidase (EC 1.1.3.21)
    and 1 more
    Fatty Acids, Lipids, and Isoprenoids Phospholipids Glycerolipid and Glycerophospholipid Metabolism in Bacteria  Alpha-glycerophosphate oxidase (EC 1.1.3.21)
  • PFAM:
    NADP_Rossmann (CL0063) DAO; FAD dependent oxidoreductase (PF01266; HMM-score: 188.5)
    and 9 more
    Fer2_BFD (CL0667) DAO_C; C-terminal domain of alpha-glycerophosphate oxidase (PF16901; HMM-score: 64.3)
    NADP_Rossmann (CL0063) FAD_binding_2; FAD binding domain (PF00890; HMM-score: 21.7)
    FAD_oxidored; FAD dependent oxidoreductase (PF12831; HMM-score: 20.2)
    FAD_binding_3; FAD binding domain (PF01494; HMM-score: 20.1)
    Pyr_redox_2; Pyridine nucleotide-disulphide oxidoreductase (PF07992; HMM-score: 15.7)
    GIDA; Glucose inhibited division protein A (PF01134; HMM-score: 15.1)
    Pyr_redox; Pyridine nucleotide-disulphide oxidoreductase (PF00070; HMM-score: 12.7)
    NAD_binding_8; NAD(P)-binding Rossmann-like domain (PF13450; HMM-score: 12.7)
    Saposin_like (CL0707) SapB_1; Saposin-like type B, region 1 (PF05184; HMM-score: 10.9)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic Membrane
    • Cytoplasmic Score: 0.04
    • Cytoplasmic Membrane Score: 9.96
    • Cellwall Score: 0
    • Extracellular Score: 0
    • Internal Helix: 1
  • DeepLocPro: Cytoplasmic Membrane
    • Cytoplasmic Score: 0.3955
    • Cytoplasmic Membrane Score: 0.5645
    • Cell wall & surface Score: 0.0009
    • Extracellular Score: 0.0391
  • SignalP: Signal peptide TAT(Tat/SPI) length 40 aa
    • SP(Sec/SPI): 0.23359
    • TAT(Tat/SPI): 0.486981
    • LIPO(Sec/SPII): 0.014889
    • Cleavage Site: CS pos: 40-41. AAA-SG. Pr: 0.3443
  • predicted transmembrane helices (TMHMM): 1

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MEFSKKTRELSIKKMQERTLDLLIIGGGITGAGVALQAAASGLETGLIEMQDFAEGTSSRSTKLVHGGLRYLKQFDVEVVSDTVSERAVVQQIAPHIPKPDPMLLPVYDEDGATFSLFRLKVAMDLYDLLAGVSNTPTANKVLSKDQVLERQPNLKKEGLVGGGVYLDFRNNDARLVIENIKRANQDGALIANHVKAEGFLFDESGKITGVVARDLLTDQVFEIKARLVINTTGPWSDKVRNLSNKGTQFSQMRPTKGVHLVVDSSKIKVSQPVYFDTGLGDGRMVFVLPRENKTYFGTTDTDYTGDLEHPKVTQEDVDYLLGIVNNRFPESNITIDDIESSWAGLRPLIAGNSASDYNGGNNGTISDESFDNLIATVESYLSKEKTREDVESAVSKLESSTSEKHLDPSAVSRGSSLDRDDNGLLTLAGGKITDYRKMAEGAMERVVDILKAEFDRSFKLINSKTYPVSGGELNPANVDSEIEAFAQLGVSRGLDSKEAHYLANLYGSNAPKVFALAHSLEQAPGLSLADTLSLHYAMRNELALSPVDFLLRRTNHMLFMRDSLDSIVEPVLDEMGRFYDWTEEEKATYRADVEAALANNDLAELKN

⊟Experimental data[edit | edit source]

  • protein localization:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator: CcpA regulon
    CcpA(TF)important in Global catabolite repression; RegPrecise 

⊟Expression data[edit | edit source]

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You can add further information about the gene and protein here. [edit]

⊟Literature[edit | edit source]

⊟References[edit | edit source]

⊟Relevant publications[edit | edit source]

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