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serotype 4
D39
serotype 2
D39V
serotype 2
Hungary19A-6
serotype 19A
EF3030
serotype 19F
670-6B
serotype 6B
6A-10
serotype 6A
70585
serotype 5
A026
serotype 19F
A66
serotype 3
AP200
serotype 11A
ASP0581
serotype 12F
ATCC 49619
serotype 19F
ATCC 700669
serotype 23F
BVJ1JL
serotype 1
CGSP14
serotype 14
G54
serotype 19F
HU-OH
serotype 3
Hu15
serotype 19A
Hu17
serotype 19A
INV104
serotype 1
INV200
serotype 14
JJA
serotype 14
MDRSPN001
serotype 19F
NCTC7466
serotype 2
NU83127
serotype 4
OXC141
serotype 3
P1031
serotype 1
R6
serotype 2
SP49
serotype 19A
SPN032672
serotype 1
SPN034156
serotype 3
SPN034183
serotype 3
SPN994038
serotype 3
SPN994039
serotype 3
SPNA45
serotype 3
ST556
serotype 19F
TCH8431/19A
serotype 19A
Taiwan19F-14
serotype 19F
Xen35
serotype 4
gamPNI0373
serotype 1

NCBI: 02-MAY-2018

⊟Summary[edit | edit source]

  • organism: Streptococcus pneumoniae D39
  • locus tag: SPD_2037 [new locus tag: SPD_RS10775 ]
  • pan locus tag?: PNEUPAN003905000
  • symbol: cysK
  • pan gene symbol?: cysK
  • synonym:
  • product: cysteine synthase A

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SPD_2037 [new locus tag: SPD_RS10775 ]
  • symbol: cysK
  • product: cysteine synthase A
  • replicon: chromosome
  • strand: +
  • coordinates: 2015731..2016651
  • length: 921
  • essential: unknown

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    ATGTCTATTTATAACAACATTACTGAATTAATCGGTCAAACACCGATTGTTAAACTTAAC
    AACATCGTGCCAGAAGGTGCTGCAGACGTCTATATAAAGCTTGAAGCATTTAATCCTGGT
    TCATCTGTAAAAGACCGTATTGCCCTTAGCATGATTGAAAAAGCTGAACAAGATGGTATT
    CTGAAACTTGGTTCTACTATTGTTGAAGCAACAAGTGGAAACACCGGTATTGGACTTTCA
    TGGGTAGGTGCTGCTAAAGGGTATAAAGTCGTCATCGTTATGCCTGAAACTATGAGTGTA
    GAACGACGTAAAATTATCCAAGCTTATGGTGCTGAACTCGTCCTAACTCCTGGTAGCGAG
    GGAATGAAAGGTGCTATTGCTAAGGCTCAAGAAATCGCTGCTGAACGTGATGGTTTCCTT
    CCTCTTCAATTTGACAATCCAGCTAATCCAGAAGTACACGAAAGAACAACAGGAGCTGAG
    ATACTAGCTGCTTTCGGTAAAGATGGATTAGATGCCTTTGTTGCTGGAGTAGGTACTGGT
    GGAACGATTTCTGGTGTTTCTCATGCACTCAAATCAGAAAATTCTAACATTCAAGTTTTT
    GCAGTAGAAGCAGATGAATCTGCTATTCTATCTGGTGAAAAACCTGGTCCTCACAAAATT
    CAAGGTATCTCAGCTGGATTTATTCCTGATACACTTGATACTAAAGCCTATGATGGTATC
    GTTCGTGTAACATCAGATGACGCTCTTGCACTCGGACGTGAAATTGGTGGAAAAGAAGGC
    TTCCTTGTAGGGATTTCCTCAGCTGCAGCTATCTACGGAGCCATCGAGGTTGCCAAAAAA
    TTAGGTACAGGTAAAAAAGTCCTTGCCCTAGCACCAGATAACGGTGAACGTTATCTCTCT
    ACAGCACTTTATGAATTGTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    921

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SPD_2037 [new locus tag: SPD_RS10775 ]
  • symbol: CysK
  • description: cysteine synthase A
  • length: 306
  • theoretical pI: 4.68169
  • theoretical MW: 31873.1
  • GRAVY: 0.0647059

⊟Function[edit | edit source]

  • reaction:
    EC 2.5.1.47?  ExPASy
    Cysteine synthase O-acetyl-L-serine + hydrogen sulfide = L-cysteine + acetate
  • TIGRFAM:
    Metabolism Amino acid biosynthesis Serine family cysteine synthase A (TIGR01139; EC 2.5.1.47; HMM-score: 428.2)
    Metabolism Amino acid biosynthesis Serine family cysteine synthase (TIGR01136; EC 2.5.1.47; HMM-score: 405.7)
    and 14 more
    Metabolism Amino acid biosynthesis Serine family cysteine synthase B (TIGR01138; EC 2.5.1.47; HMM-score: 277.1)
    Metabolism Amino acid biosynthesis Serine family cystathionine beta-synthase (TIGR01137; EC 4.2.1.22; HMM-score: 274.9)
    Cellular processes Cellular processes Biosynthesis of natural products 2,3-diaminopropionate biosynthesis protein SbnA (TIGR03945; HMM-score: 227)
    Metabolism Amino acid biosynthesis Pyruvate family threonine ammonia-lyase (TIGR01127; EC 4.3.1.19; HMM-score: 77.6)
    Metabolism Amino acid biosynthesis Pyruvate family threonine ammonia-lyase, biosynthetic (TIGR01124; EC 4.3.1.19; HMM-score: 64.2)
    Metabolism Amino acid biosynthesis Pyruvate family threonine dehydratase (TIGR02079; EC 4.3.1.19; HMM-score: 54.8)
    Metabolism Amino acid biosynthesis Aspartate family threonine synthase (TIGR00260; EC 4.2.3.1; HMM-score: 41.6)
    ectoine utilization protein EutB (TIGR02991; HMM-score: 40.8)
    diaminopropionate ammonia-lyase (TIGR03528; EC 4.3.1.15; HMM-score: 28)
    Metabolism Energy metabolism Other diaminopropionate ammonia-lyase family (TIGR01747; EC 4.3.1.15; HMM-score: 27.3)
    Unknown function Enzymes of unknown specificity pyridoxal-phosphate dependent TrpB-like enzyme (TIGR01415; HMM-score: 21.1)
    Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Other cysteate synthase (TIGR03844; EC 2.5.1.76; HMM-score: 17)
    Metabolism Energy metabolism Methanogenesis cysteate synthase (TIGR03844; EC 2.5.1.76; HMM-score: 17)
    pyridoxal phosphate-dependent enzymes, D-cysteine desulfhydrase family (TIGR01275; HMM-score: 14.4)
  • TheSEED  :
    • Cysteine synthase (EC 2.5.1.47)
    Amino Acids and Derivatives Lysine, threonine, methionine, and cysteine Cysteine Biosynthesis  Cysteine synthase (EC 2.5.1.47)
    and 1 more
    Amino Acids and Derivatives Lysine, threonine, methionine, and cysteine Methionine Biosynthesis  Cysteine synthase (EC 2.5.1.47)
  • PFAM:
    NADP_Rossmann (CL0063) PALP; Pyridoxal-phosphate dependent enzyme (PF00291; HMM-score: 220.3)
    and 1 more
    PRMT5; PRMT5 arginine-N-methyltransferase (PF05185; HMM-score: 12.6)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.007767
    • TAT(Tat/SPI): 0.000161
    • LIPO(Sec/SPII): 0.0003
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MSIYNNITELIGQTPIVKLNNIVPEGAADVYIKLEAFNPGSSVKDRIALSMIEKAEQDGILKLGSTIVEATSGNTGIGLSWVGAAKGYKVVIVMPETMSVERRKIIQAYGAELVLTPGSEGMKGAIAKAQEIAAERDGFLPLQFDNPANPEVHERTTGAEILAAFGKDGLDAFVAGVGTGGTISGVSHALKSENSNIQVFAVEADESAILSGEKPGPHKIQGISAGFIPDTLDTKAYDGIVRVTSDDALALGREIGGKEGFLVGISSAAAIYGAIEVAKKLGTGKKVLALAPDNGERYLSTALYEL

⊟Experimental data[edit | edit source]

  • protein localization:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator: CmbR* (activation (repression)) regulon
    CmbR*(TF)important in Cysteine metabolism; RegPrecise 

⊟Expression data[edit | edit source]

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

⊟Relevant publications[edit | edit source]

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