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serotype 4
D39
serotype 2
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serotype 2
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EF3030
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serotype 6B
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serotype 23F
BVJ1JL
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CGSP14
serotype 14
G54
serotype 19F
HU-OH
serotype 3
Hu15
serotype 19A
Hu17
serotype 19A
INV104
serotype 1
INV200
serotype 14
JJA
serotype 14
MDRSPN001
serotype 19F
NCTC7466
serotype 2
NU83127
serotype 4
OXC141
serotype 3
P1031
serotype 1
R6
serotype 2
SP49
serotype 19A
SPN032672
serotype 1
SPN034156
serotype 3
SPN034183
serotype 3
SPN994038
serotype 3
SPN994039
serotype 3
SPNA45
serotype 3
ST556
serotype 19F
TCH8431/19A
serotype 19A
Taiwan19F-14
serotype 19F
Xen35
serotype 4
gamPNI0373
serotype 1

NCBI: 02-FEB-2021

⊟Summary[edit | edit source]

  • organism: Streptococcus pneumoniae D39
  • locus tag: SPD_RS08955 [old locus tag: SPD_1680 ]
  • pan locus tag?: PNEUPAN003398000
  • symbol: SPD_RS08955
  • pan gene symbol?: birA
  • synonym:
  • product: bifunctional biotin--[acetyl-CoA-carboxylase] ligase/biotin operon repressor BirA

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SPD_RS08955 [old locus tag: SPD_1680 ]
  • symbol: SPD_RS08955
  • product: bifunctional biotin--[acetyl-CoA-carboxylase] ligase/biotin operon repressor BirA
  • replicon: chromosome
  • strand: +
  • coordinates: 1696528..1697463
  • length: 936
  • essential: unknown

⊟Accession numbers[edit | edit source]

  • Location: NC_008533 (1696528..1697463) NCBI
  • BioCyc: G1G6V-1830 BioCyc
  • MicrobesOnline: see SPD_1680
  • PneumoBrowse for strain D39V: SPV_1680 PneumoBrowse

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    ATGAAATCCTACCAAGCTGTCTACCAAATCCTATCTAAAGAAACCGACTATATCAGCGGA
    GAAAAAATCGCAGAAAAACTATCCCTAAGCCGAACATCAATTTGGAAAGCTATCAAGCGA
    CTAGAACAAGAAGGCATTGAAATTAATAGTATCAAAAATAGAGGATATAAACTAATGAAT
    GGTGACCTTATTCTTCCAGAGATTCTAGAAGAAAATCTTCCAATTAAAGTCAGCTTTAAA
    CCCGAAACAAAATCAACACAACTAGATGCAAAAGAAGCAATTGATTTAGGCCATGAAGCA
    AATACTCTCTATCTAGCTTCCTATCAAACAGCAGGCCGAGGCCGTTTTCAACGTTCCTTC
    TACTCACCACAAGGTGGAATTTATATGACACTCCATCTTAAACCAAATCTCCCCTATGAC
    AAATTACCATCCTACACACTACTTGTAGCTGGAGCTGTCTACAAAGCCATTAAGAACCTA
    ACTTTAATAGATGTCGATATAAAATGGGTCAATGATATCTATCTAAACAATCATAAAATT
    GGAGGAATCCTTACTGAAGCAATGACCTCTGTAGAAACTGGCTTAGTCACAGATATCATT
    ATTGGAGTAGGTATCAATTTCACTATTAAAGACTTCCCTCAGGAATTAAAAGAAAAAGCT
    GCCAGCTTATTTAAAGCTACAGCTCCTATAACAAGGAATGAATTGATCATAGAAATCTGG
    CGTGCTTTCTTCGAAACACCAGCAGAAGAGCTATTATACCTATACAAAAAACAGTCATTC
    ATTCTAGGAAAAGAAGTCACTTTCACACTAGAGCAAAAAGACTACAAGGGACTTGCTAAA
    GACATCTCAGAAAATGGAAAACTTTTAGTTCAATGTGATAACGGAAAAGAAATCTGGCTA
    AATAGTGGCGAAATTTCTCTCAATAGTTGGAAGTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    936

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SPD_RS08955 [old locus tag: SPD_1680 ]
  • symbol: SPD_RS08955
  • description: bifunctional biotin--[acetyl-CoA-carboxylase] ligase/biotin operon repressor BirA
  • length: 311
  • theoretical pI: 7.56437
  • theoretical MW: 35242.4
  • GRAVY: -0.230547

⊟Function[edit | edit source]

  • reaction:
    EC 6.3.4.15?  ExPASy
    Biotin--[acetyl-CoA-carboxylase] ligase ATP + biotin + apo-[acetyl-CoA:carbon-dioxide ligase (ADP-forming)] = AMP + diphosphate + [acetyl-CoA:carbon-dioxide ligase (ADP-forming)]
  • TIGRFAM:
    Genetic information processing Protein fate Protein modification and repair biotin--[acetyl-CoA-carboxylase] ligase (TIGR00121; EC 6.3.4.15; HMM-score: 167)
    and 4 more
    Signal transduction Regulatory functions DNA interactions biotin operon repressor (TIGR00122; HMM-score: 43.7)
    phosphonate utilization associated transcriptional regulator (TIGR03338; HMM-score: 12.4)
    Signal transduction Regulatory functions DNA interactions CRISPR locus-related DNA-binding protein (TIGR01884; HMM-score: 12.3)
    Signal transduction Regulatory functions DNA interactions trehalose operon repressor (TIGR02404; HMM-score: 11.8)
  • TheSEED: see SPD_1680
  • PFAM:
    tRNA_synt_II (CL0040) BPL_LplA_LipB; Biotin/lipoate A/B protein ligase family (PF03099; HMM-score: 72)
    and 16 more
    HTH (CL0123) HTH_11; HTH domain (PF08279; HMM-score: 54.5)
    TRB (CL0206) BPL_C; Biotin protein ligase C terminal domain (PF02237; HMM-score: 44.8)
    HTH (CL0123) HTH_24; Winged helix-turn-helix DNA-binding (PF13412; HMM-score: 26)
    Fe_dep_repress; Iron dependent repressor, N-terminal DNA binding domain (PF01325; HMM-score: 18.6)
    GntR; Bacterial regulatory proteins, gntR family (PF00392; HMM-score: 17.7)
    HTH_23; Homeodomain-like domain (PF13384; HMM-score: 17.5)
    MarR; MarR family (PF01047; HMM-score: 17.2)
    HTH_36; Helix-turn-helix domain (PF13730; HMM-score: 16.3)
    HTH_1; Bacterial regulatory helix-turn-helix protein, lysR family (PF00126; HMM-score: 14.9)
    HTH_41; Helix-turn-helix domain (PF14502; HMM-score: 14.2)
    Mga; Mga helix-turn-helix domain (PF05043; HMM-score: 14.1)
    TrmB; Sugar-specific transcriptional regulator TrmB (PF01978; HMM-score: 14)
    HTH_29; Winged helix-turn helix (PF13551; HMM-score: 13.9)
    HTH_Crp_2; Crp-like helix-turn-helix domain (PF13545; HMM-score: 13)
    HTH_AsnC-type; AsnC-type helix-turn-helix domain (PF13404; HMM-score: 12.3)
    HTH_28; Helix-turn-helix domain (PF13518; HMM-score: 11.9)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:
  • genes regulated by BirA, TF important in Biotin biosynthesis: in TIGR4

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 9.97
    • Cytoplasmic Membrane Score: 0
    • Cellwall Score: 0.01
    • Extracellular Score: 0.02
    • Internal Helices: 0
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.005547
    • TAT(Tat/SPI): 0.000279
    • LIPO(Sec/SPII): 0.000566
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MKSYQAVYQILSKETDYISGEKIAEKLSLSRTSIWKAIKRLEQEGIEINSIKNRGYKLMNGDLILPEILEENLPIKVSFKPETKSTQLDAKEAIDLGHEANTLYLASYQTAGRGRFQRSFYSPQGGIYMTLHLKPNLPYDKLPSYTLLVAGAVYKAIKNLTLIDVDIKWVNDIYLNNHKIGGILTEAMTSVETGLVTDIIIGVGINFTIKDFPQELKEKAASLFKATAPITRNELIIEIWRAFFETPAEELLYLYKKQSFILGKEVTFTLEQKDYKGLAKDISENGKLLVQCDNGKEIWLNSGEISLNSWK

⊟Experimental data[edit | edit source]

  • protein localization:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator:

⊟Expression data[edit | edit source]

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

⊟Relevant publications[edit | edit source]

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