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TIGR4
serotype 4
D39
serotype 2
D39V
serotype 2
Hungary19A-6
serotype 19A
EF3030
serotype 19F
670-6B
serotype 6B
6A-10
serotype 6A
70585
serotype 5
A026
serotype 19F
A66
serotype 3
AP200
serotype 11A
ASP0581
serotype 12F
ATCC 49619
serotype 19F
ATCC 700669
serotype 23F
BM6001
serotype 19F
BVJ1JL
serotype 1
CGSP14
serotype 14
G54
serotype 19F
HU-OH
serotype 3
Hu15
serotype 19A
Hu17
serotype 19A
INV104
serotype 1
INV200
serotype 14
JJA
serotype 14
MDRSPN001
serotype 19F
NCTC7465
serotype 1
NCTC7466
serotype 2
NU83127
serotype 4
OXC141
serotype 3
P1031
serotype 1
R6
serotype 2
SP49
serotype 19A
SPN032672
serotype 1
SPN034156
serotype 3
SPN034183
serotype 3
SPN994038
serotype 3
SPN994039
serotype 3
SPNA45
serotype 3
ST556
serotype 19F
TCH8431/19A
serotype 19A
Taiwan19F-14
serotype 19F
Xen35
serotype 4
gamPNI0373
serotype 1

NCBI: 30-JAN-2014

⊟Summary[edit | edit source]

  • organism: Streptococcus pneumoniae G54
  • locus tag: SPG_1150
  • pan locus tag?:
  • symbol: SPG_1150
  • pan gene symbol?: —
  • synonym:
  • product: 3-isopropylmalate dehydrogenase, point mutation

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SPG_1150
  • symbol: SPG_1150
  • product: 3-isopropylmalate dehydrogenase, point mutation
  • replicon: chromosome
  • strand: -
  • coordinates: 1126639..1126794
  • length: 156
  • essential: unknown

⊟Accession numbers[edit | edit source]

  • Location: CP001015 (1126639..1126794) NCBI
  • BioCyc:
  • MicrobesOnline: 5756419 MicrobesOnline
  • PneumoBrowse for strain D39V:

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    ATGGCAAAGAAAATAGTAGCTCTAGCAGGAGACGGAATTGGCCCAGAAATCATGGAGGTT
    GGTTTAGAAGTTCTGGAGGCTCTAGCTGAAAAAACAGGTTTTGACTATGAGATTGACAGA
    CGACCGTTCGGAGGTGCAGATATTGATGCAGCATGA
    60
    120
    156

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SPG_1150
  • symbol: SPG_1150
  • description: 3-isopropylmalate dehydrogenase, point mutation
  • length: 51
  • theoretical pI: 3.97646
  • theoretical MW: 5364.09
  • GRAVY: 0.135294

⊟Function[edit | edit source]

  • reaction:
    EC 1.1.1.85?  ExPASy
    3-isopropylmalate dehydrogenase (2R,3S)-3-isopropylmalate + NAD+ = 4-methyl-2-oxopentanoate + CO2 + NADH
  • TIGRFAM:
    Metabolism Amino acid biosynthesis Pyruvate family 3-isopropylmalate dehydrogenase (TIGR00169; EC 1.1.1.85; HMM-score: 42.7)
    and 4 more
    Metabolism Energy metabolism Other tartrate dehydrogenase (TIGR02089; EC 1.1.1.93; HMM-score: 26.7)
    Metabolism Energy metabolism TCA cycle isocitrate dehydrogenase (TIGR02924; EC 1.1.1.-; HMM-score: 17.5)
    isopropylmalate/isohomocitrate dehydrogenases (TIGR02088; HMM-score: 15.3)
    Metabolism Energy metabolism TCA cycle isocitrate dehydrogenase, NAD-dependent (TIGR00175; EC 1.1.1.41; HMM-score: 11.1)
  • TheSEED  :
    • 3-isopropylmalate dehydrogenase (EC 1.1.1.85)
    Amino Acids and Derivatives Branched-chain amino acids Branched-Chain Amino Acid Biosynthesis  3-isopropylmalate dehydrogenase (EC 1.1.1.85)
    and 1 more
    Amino Acids and Derivatives Branched-chain amino acids Leucine Biosynthesis  3-isopropylmalate dehydrogenase (EC 1.1.1.85)
  • PFAM:
    Iso_DH (CL0270) Iso_dh; Isocitrate/isopropylmalate dehydrogenase (PF00180; HMM-score: 33.9)
    and 1 more
    CNF1_YfiH (CL0663) Cu-oxidase_4; Multi-copper polyphenol oxidoreductase laccase (PF02578; HMM-score: 11.3)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 9.97
    • Cytoplasmic Membrane Score: 0
    • Cellwall Score: 0.01
    • Extracellular Score: 0.02
    • Internal Helices: 0
  • DeepLocPro: Cytoplasmic
    • Cytoplasmic Score: 0.9723
    • Cytoplasmic Membrane Score: 0.0015
    • Cell wall & surface Score: 0
    • Extracellular Score: 0.0261
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.015094
    • TAT(Tat/SPI): 0.000686
    • LIPO(Sec/SPII): 0.001198
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

  • GI:
  • RefSeq: ACF55269 NCBI
  • UniProt:

⊟Protein sequence[edit | edit source]

  • MAKKIVALAGDGIGPEIMEVGLEVLEALAEKTGFDYEIDRRPFGGADIDAA

⊟Experimental data[edit | edit source]

  • protein localization:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator:

⊟Transcription[edit | edit source]

  • transcription start site:

⊟Expression data[edit | edit source]

  • PneumoExpress for strain D39V:

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You can add further information about the gene and protein here. [edit]

⊟Literature[edit | edit source]

⊟References[edit | edit source]

⊟Relevant publications[edit | edit source]

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