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serotype 1

NCBI: 30-JAN-2014

⊟Summary[edit | edit source]

  • organism: Streptococcus pneumoniae G54
  • locus tag: SPG_1971 [new locus tag: SPG_RS09855 ]
  • pan locus tag?: PNEUPAN003690000
  • symbol: nagA
  • pan gene symbol?: nagA
  • synonym:
  • product: N-acetylglucosamine-6-phosphate deacetylase

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SPG_1971 [new locus tag: SPG_RS09855 ]
  • symbol: nagA
  • product: N-acetylglucosamine-6-phosphate deacetylase
  • replicon: chromosome
  • strand: -
  • coordinates: 1866332..1867483
  • length: 1152
  • essential: unknown

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    ATGCCTAACTATATTAAAGCGGATCAGTTTTTCTACCCACACGGAGTTCGTCGAGGTGGT
    TACTTGGAACTTGTGGACGGCAAGTTTGGGAAACATGTAGAGCAGATTCCTGAAGGGGCT
    GAGGTGATTGACTATACAGGTTATAGCATTGCCCCAGGTCTTGTGGATACTCATATTCAT
    GGATATGCAGGTGTANATGTGATGGACAACAACATTGAAGGTACATTGCATACTATGAGT
    GAAGGACTTCTTAGTACCGGTGTTACCAGTTTCTTACCCACAACTTTAACAGCCACTTAT
    GAGCAATTGCTTGCAGTCACTGAAAATCTTGGAAACCATTATAAAGAAGCAACAGGTGCT
    AAGATTCGTGGGATTTATTATGAAGGTCCATATTTCACAGAAACTTTTAAGGGGGCACAA
    AATCCAACTTATATGAGAGACCCGGGTGTTGAGGAGTTTCATTCTTGGCAAAAAGCGGCA
    AATGGCTTGCTTAATAAAATTGCCCTTGCACCAGAACGTGATGGGGTGGAAGACTTTGTT
    CGTACAGTTACGGGCGAAGGTGTGACGGTTGCTCTTGGACATTCAAACGCGACTTTTGAT
    GAAGCCAAAAAAGCAGTCGATGCTGGAGCGAGTGTTTGGGTGCATGCCTACAATGGAATG
    CGTGGGTTGACTCACCGTGAATTGGGTATGGTTGGAGCCATGTACCAATTGCCACATACC
    TATGCAGAGTTGATCTGTGATGGTCACCACGTAGATCCAAAGGCTTGCGAAATTCTTATC
    AAACAAAAAGGAACAGAAAACATCGCTCTTATCACAGACTGTATGACAGCTGGGGGATTG
    GAAGACGGAGATTATATGTTGGGAGAATTCCCAGTTGTCGTTGCAAATGGAACTGCACGC
    CTCAAATCGACAGGTAACTTGGCAGGTTCTATCCTCAAACTCAAAGATGGTTTGAAGAAT
    GTGGTCGAATGGGGAATTGCGAATCCGCATGAAGCAGTCATGATGGCCAGCTTCAACCCA
    GCTAAATCCGTTCACATCGATGACGTCTGTGGCCAAATCCGTGAAGGCTACGACGCTGAC
    TTCATCGTATTAGATAAAGATTTGGAATTGGTAGCAACCTACCTAGATGGCGTAAAACGT
    TATCAAGCATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1152

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SPG_1971 [new locus tag: SPG_RS09855 ]
  • symbol: NagA
  • description: N-acetylglucosamine-6-phosphate deacetylase
  • length: 383
  • theoretical pI: 5.02694
  • theoretical MW: 41581.7
  • GRAVY: -0.177749

⊟Function[edit | edit source]

  • reaction:
    EC 3.5.1.25?  ExPASy
    N-acetylglucosamine-6-phosphate deacetylase N-acetyl-D-glucosamine 6-phosphate + H2O = D-glucosamine 6-phosphate + acetate
  • TIGRFAM:
    Metabolism Central intermediary metabolism Amino sugars N-acetylglucosamine-6-phosphate deacetylase (TIGR00221; EC 3.5.1.25; HMM-score: 292.9)
    and 11 more
    Metabolism Purines, pyrimidines, nucleosides, and nucleotides Other guanine deaminase (TIGR02967; EC 3.5.4.3; HMM-score: 36.7)
    Metabolism Energy metabolism Other phosphonate metabolism protein PhnM (TIGR02318; HMM-score: 35.6)
    Metabolism Energy metabolism Amino acids and amines imidazolonepropionase (TIGR01224; EC 3.5.2.7; HMM-score: 35.1)
    allantoinase (TIGR03178; EC 3.5.2.5; HMM-score: 31.1)
    Metabolism Purines, pyrimidines, nucleosides, and nucleotides Pyrimidine ribonucleotide biosynthesis dihydroorotase, multifunctional complex type (TIGR00857; EC 3.5.2.3; HMM-score: 27.6)
    Metabolism Purines, pyrimidines, nucleosides, and nucleotides Salvage of nucleosides and nucleotides adenine deaminase (TIGR01178; EC 3.5.4.2; HMM-score: 26.1)
    dihydropyrimidinase (TIGR02033; EC 3.5.2.2; HMM-score: 24.7)
    Genetic information processing Protein fate Degradation of proteins, peptides, and glycopeptides beta-aspartyl peptidase (TIGR01975; EC 3.4.19.5; HMM-score: 19.9)
    Hypothetical proteins Conserved putative amidohydrolase, EF_0837/AHA_3915 family (TIGR03583; HMM-score: 19.1)
    formylmethanofuran dehydrogenase subunit A (TIGR03121; EC 1.2.99.5; HMM-score: 14.7)
    putative selenium metabolism protein SsnA (TIGR03314; HMM-score: 11.3)
  • TheSEED  :
    • N-acetylglucosamine-6-phosphate deacetylase (EC 3.5.1.25)
    Carbohydrates Aminosugars Chitin and N-acetylglucosamine utilization  N-acetylglucosamine-6-phosphate deacetylase (EC 3.5.1.25)
    and 2 more
    Cell Wall and Capsule Capsular and extracellular polysacchrides Sialic Acid Metabolism  N-acetylglucosamine-6-phosphate deacetylase (EC 3.5.1.25)
    Cell Wall and Capsule Cell Wall and Capsule - no subcategory UDP-N-acetylmuramate from Fructose-6-phosphate Biosynthesis  N-acetylglucosamine-6-phosphate deacetylase (EC 3.5.1.25)
  • PFAM:
    Amidohydrolase (CL0034) Amidohydro_1; Amidohydrolase family (PF01979; HMM-score: 72.9)
    and 1 more
    Amidohydro_3; Amidohydrolase family (PF07969; HMM-score: 45.6)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • DeepLocPro: Cytoplasmic
    • Cytoplasmic Score: 0.9993
    • Cytoplasmic Membrane Score: 0.0001
    • Cell wall & surface Score: 0
    • Extracellular Score: 0.0006
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.004751
    • TAT(Tat/SPI): 0.000483
    • LIPO(Sec/SPII): 0.000485
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

  • GI:
  • RefSeq: ACF55866 NCBI
  • UniProt:

⊟Protein sequence[edit | edit source]

  • MPNYIKADQFFYPHGVRRGGYLELVDGKFGKHVEQIPEGAEVIDYTGYSIAPGLVDTHIHGYAGVXVMDNNIEGTLHTMSEGLLSTGVTSFLPTTLTATYEQLLAVTENLGNHYKEATGAKIRGIYYEGPYFTETFKGAQNPTYMRDPGVEEFHSWQKAANGLLNKIALAPERDGVEDFVRTVTGEGVTVALGHSNATFDEAKKAVDAGASVWVHAYNGMRGLTHRELGMVGAMYQLPHTYAELICDGHHVDPKACEILIKQKGTENIALITDCMTAGGLEDGDYMLGEFPVVVANGTARLKSTGNLAGSILKLKDGLKNVVEWGIANPHEAVMMASFNPAKSVHIDDVCGQIREGYDADFIVLDKDLELVATYLDGVKRYQA

⊟Experimental data[edit | edit source]

  • protein localization:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator: NagR* (repression) regulon
    NagR*(TF)important in N-acetylglucosamine utilization; RegPrecise 

⊟Expression data[edit | edit source]

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

⊟Relevant publications[edit | edit source]

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