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PangenomeTIGR4
serotype 4
D39
serotype 2
D39V
serotype 2
Hungary19A-6
serotype 19A
EF3030
serotype 19F
670-6B
serotype 6B
6A-10
serotype 6A
70585
serotype 5
A026
serotype 19F
A66
serotype 3
AP200
serotype 11A
ASP0581
serotype 12F
ATCC 49619
serotype 19F
ATCC 700669
serotype 23F
BM6001
serotype 19F
BVJ1JL
serotype 1
CGSP14
serotype 14
G54
serotype 19F
HU-OH
serotype 3
Hu15
serotype 19A
Hu17
serotype 19A
INV104
serotype 1
INV200
serotype 14
JJA
serotype 14
MDRSPN001
serotype 19F
NCTC7465
serotype 1
NCTC7466
serotype 2
NU83127
serotype 4
OXC141
serotype 3
P1031
serotype 1
R6
serotype 2
SP49
serotype 19A
SPN032672
serotype 1
SPN034156
serotype 3
SPN034183
serotype 3
SPN994038
serotype 3
SPN994039
serotype 3
SPNA45
serotype 3
ST556
serotype 19F
TCH8431/19A
serotype 19A
Taiwan19F-14
serotype 19F
Xen35
serotype 4
gamPNI0373
serotype 1

NCBI: 31-JAN-2014

⊟Summary[edit | edit source]

  • organism: Streptococcus pneumoniae Hungary19A-6
  • locus tag: SPH_0618 [new locus tag: SPH_RS02990 ]
  • pan locus tag?: PNEUPAN001374000
  • symbol: SPH_0618
  • pan gene symbol?: hsdM
  • synonym:
  • product: type I restriction enzyme EcoEI M protein (M.EcoEI)

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SPH_0618 [new locus tag: SPH_RS02990 ]
  • symbol: SPH_0618
  • product: type I restriction enzyme EcoEI M protein (M.EcoEI)
  • replicon: chromosome
  • strand: -
  • coordinates: 569612..571075
  • length: 1464
  • essential: unknown

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    ATGTCAATTACATCATTTGTAAAAAGAATTCAAGATATCACTCGAAACGATGCTGGTGTT
    AATGGTGATGCTCAACGTATTGAGCAAATGTCTTGGTTATTATTCTTAAAAATTTATGAT
    AGCCGTGAAATGGTTTGGGAATTAGAAGAAGACGAGTATGAGTCAATTATCCCAAAGGAA
    TTAAAATGGCGAAATTGGGCTCATGCTCAAAATGGGGAACGGGTATTGACAGGCGATGAA
    TTACTTGATTTTGTCAATAACAAGTTATTCAAAGAGTTGAAAGAGCTTGAAATAACTTCA
    AATATGCCTATTCGAAAAACGATTGTTAAATCAGCTTTTGAAGATGCGAACAACTATATG
    AAAAATGGCGTCTTGTTACGCCAAGTCATCAATGTTATTGATGAAGTTGATTTCAATAGC
    CCTGAAGATCGTCATTCGTTTAATGATATTTACGAAAAAATTCTTAAAGATATTCAAAAT
    GCTGGGAACTCAGGAGAATTTTATACACCACGTGCAGCGACTGATTTTATTGCCGAAGTT
    CTTGACCCAAAACTTGGAGAATCAATGGCAGACCTTGCTTGCGGAACAGGAGGCTTCTTG
    ACTTCGACTCTGAACCGTTTAAGTAGTCAACGTAAAACTAGTGAAGATACCAAAAAATAT
    AATACAGCTGTTTTTGGTATTGAAAAGAAAGCATTTCCTCATCTTTTAGCAGTTACAAAT
    CTGTTTCTTCACGAAATTGATGACCCTAAAATTGTTCATGGAAATACTTTGGAGAAAAAT
    GTTCGTGAATATACGGATGATGAAAAATTTGACATTATTATGATGAATCCACCTTTTGGA
    GGGTCAGAATTAGAAACAATAAAAAATAACTTTCCAGCAGAATTACGGAGTTCTGAAACA
    GCTGATTTATTTATGGCTGTCATTATGTATCGTTTGAAAGAAAATGGTCGTGTTGGAGTT
    ATTTTACCTGATGGTTTTCTATTTGGTGAAGGTGTAAAAACTCGCTTGAAACAAAAACTG
    GTAGATGAGTTCAACTTGCATACGATTATTAGGTTGCCTCATAGTGTCTTTGCACCGTAT
    ACAGGAATCCATACGAACATTCTTTTCTTTGATAAAACAAAGAAAACAGAAGAAACTTGG
    TTTTATCGTTTAGATATGCCAGATGGTTATAAAAATTTCTCGAAAACTAAGCCGATGAAG
    TCAGAACACTTCAATCCTGTTCGTGACTGGTGGGAAAATCGTGAAGAGATTCTGGAAGGT
    AAGTTCTACAAATCTAAATCATTTACACCTAGTGAATTGGCTGAGTTGAATTATAATTTA
    GACCAGTGTGGCTTTCCAAAAGAGGAAGAGGAAATCTTAAATCCCTTTGAGTTGATTCAG
    AATTATCAAGCGGAAAGAGCAACTTTAAATCATAAGATTGATAATGTATTAGCTGATATT
    TTGCAGTTGTTGGAGGACAAATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1320
    1380
    1440
    1464

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SPH_0618 [new locus tag: SPH_RS02990 ]
  • symbol: SPH_0618
  • description: type I restriction enzyme EcoEI M protein (M.EcoEI)
  • length: 487
  • theoretical pI: 4.83027
  • theoretical MW: 56479.6
  • GRAVY: -0.528131

⊟Function[edit | edit source]

  • reaction:
    EC 2.1.1.72?  ExPASy
    Site-specific DNA-methyltransferase (adenine-specific) S-adenosyl-L-methionine + adenine in DNA = S-adenosyl-L-homocysteine + N-6-methyladenine in DNA
  • TIGRFAM:
    Genetic information processing DNA metabolism Restriction/modification type I restriction-modification system, M subunit (TIGR00497; EC 2.1.1.72; HMM-score: 82.3)
    and 2 more
    Genetic information processing DNA metabolism Restriction/modification type II restriction m6 adenine DNA methyltransferase, Alw26I/Eco31I/Esp3I family (TIGR02987; EC 2.1.1.72; HMM-score: 38.1)
    Genetic information processing Protein fate Protein modification and repair protein-(glutamine-N5) methyltransferase, release factor-specific (TIGR03534; EC 2.1.1.-; HMM-score: 23.1)
  • TheSEED  :
    • Type I restriction-modification system, DNA-methyltransferase subunit M (EC 2.1.1.72)
    DNA Metabolism DNA Metabolism - no subcategory Restriction-Modification System  Type I restriction-modification system, DNA-methyltransferase subunit M (EC 2.1.1.72)
    and 1 more
    DNA Metabolism DNA Metabolism - no subcategory Type I Restriction-Modification  Type I restriction-modification system, DNA-methyltransferase subunit M (EC 2.1.1.72)
  • PFAM:
    NADP_Rossmann (CL0063) N6_Mtase; N-6 DNA Methylase (PF02384; HMM-score: 233.1)
    and 6 more
    no clan defined HsdM_N; HsdM N-terminal domain (PF12161; HMM-score: 43.8)
    NADP_Rossmann (CL0063) MTS; Methyltransferase small domain (PF05175; HMM-score: 28.4)
    Eco57I; Eco57I restriction-modification methylase (PF07669; HMM-score: 21.4)
    MmeI_Mtase; MmeI, DNA-methyltransferase domain (PF20473; HMM-score: 21.4)
    Methyltransf_31; Methyltransferase domain (PF13847; HMM-score: 14.3)
    FtsJ; FtsJ-like methyltransferase (PF01728; HMM-score: 12.3)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • DeepLocPro: Cytoplasmic
    • Cytoplasmic Score: 0.9653
    • Cytoplasmic Membrane Score: 0.0039
    • Cell wall & surface Score: 0.0006
    • Extracellular Score: 0.0302
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.002105
    • TAT(Tat/SPI): 0.00017
    • LIPO(Sec/SPII): 0.000457
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MSITSFVKRIQDITRNDAGVNGDAQRIEQMSWLLFLKIYDSREMVWELEEDEYESIIPKELKWRNWAHAQNGERVLTGDELLDFVNNKLFKELKELEITSNMPIRKTIVKSAFEDANNYMKNGVLLRQVINVIDEVDFNSPEDRHSFNDIYEKILKDIQNAGNSGEFYTPRAATDFIAEVLDPKLGESMADLACGTGGFLTSTLNRLSSQRKTSEDTKKYNTAVFGIEKKAFPHLLAVTNLFLHEIDDPKIVHGNTLEKNVREYTDDEKFDIIMMNPPFGGSELETIKNNFPAELRSSETADLFMAVIMYRLKENGRVGVILPDGFLFGEGVKTRLKQKLVDEFNLHTIIRLPHSVFAPYTGIHTNILFFDKTKKTEETWFYRLDMPDGYKNFSKTKPMKSEHFNPVRDWWENREEILEGKFYKSKSFTPSELAELNYNLDQCGFPKEEEEILNPFELIQNYQAERATLNHKIDNVLADILQLLEDK

⊟Experimental data[edit | edit source]

  • protein localization:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator:

⊟Transcription[edit | edit source]

  • transcription start site:

⊟Expression data[edit | edit source]

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You can add further information about the gene and protein here. [edit]

⊟Literature[edit | edit source]

⊟References[edit | edit source]

⊟Relevant publications[edit | edit source]

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