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PangenomeTIGR4
serotype 4
D39
serotype 2
D39V
serotype 2
Hungary19A-6
serotype 19A
EF3030
serotype 19F
670-6B
serotype 6B
6A-10
serotype 6A
70585
serotype 5
A026
serotype 19F
A66
serotype 3
AP200
serotype 11A
ASP0581
serotype 12F
ATCC 49619
serotype 19F
ATCC 700669
serotype 23F
BVJ1JL
serotype 1
CGSP14
serotype 14
G54
serotype 19F
HU-OH
serotype 3
Hu15
serotype 19A
Hu17
serotype 19A
INV104
serotype 1
INV200
serotype 14
JJA
serotype 14
MDRSPN001
serotype 19F
NCTC7466
serotype 2
NU83127
serotype 4
OXC141
serotype 3
P1031
serotype 1
R6
serotype 2
SP49
serotype 19A
SPN032672
serotype 1
SPN034156
serotype 3
SPN034183
serotype 3
SPN994038
serotype 3
SPN994039
serotype 3
SPNA45
serotype 3
ST556
serotype 19F
TCH8431/19A
serotype 19A
Taiwan19F-14
serotype 19F
Xen35
serotype 4
gamPNI0373
serotype 1

NCBI: 31-JAN-2014

⊟Summary[edit | edit source]

  • organism: Streptococcus pneumoniae Hungary19A-6
  • locus tag: SPH_1147 [new locus tag: SPH_RS05565 ]
  • pan locus tag?: PNEUPAN002138000
  • symbol: SPH_1147
  • pan gene symbol?: nplT
  • synonym:
  • product: neopullulanase

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SPH_1147 [new locus tag: SPH_RS05565 ]
  • symbol: SPH_1147
  • product: neopullulanase
  • replicon: chromosome
  • strand: +
  • coordinates: 1055336..1057075
  • length: 1740
  • essential: unknown

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    ATGGAATTGAGTGCTATTTACCATAGGCCTGAGTCGGAGTATGCCTATCTTTATAAGGAT
    AAGAAACTCCATATTCGAATTCGAACTAAGAAAGGGGACATTGAAAGCATTAACTTGCAC
    TATGGGGACCCTTTTATCTTTATGGAGGAGTTTTATCAGGATACAAAAGAAATGGTCAAG
    ATAACTTCTGGTACCTTATTTGACCATTGGCAGGTTGAAGTGTCAGTTGACTTTGCACAT
    ATCCAGTATCTCTTTGAGATCAGAGATACAGAAGGTCAAAATATTTTGTATGGCGATAAA
    GGGTGTGTGGAAAATTCTCTAGAAAATCTTCATGCAATTGGGAATGGATTTAAGTTGCCT
    TATCTTCATGAGATTGATGCCTGCAAGGTTCCTGACTGGGTTTCAAATACGGTATGGTAT
    CAGATATTTCCTGAAAGATTTGCCAATGGCAATGCTCTATTAAACCCAGAAGGGACTTTA
    GACTGGGATTCATCTGTCACACCTAAGAGCGATGATTTCTTTGGTGGTGATTTACAGGGG
    ATTATTGATCATATGGATTACTTGCAAGACTTGGGTATTACTGGACTATATCTTTGTCCC
    ATCTTTGAATCTACAAGCAATCACAAGTACAATACGACAGATTACTTTGAAATTGACCGT
    CATTTTGGAGACAAGGAGACCTTTCGGGAACTGGTGGATCAAGCGCATCATCGTGGCATG
    AAAGTCATGCTGGATGCGGTATTTAATCATATTGGTTCGCAATCTCTTCAATGGAAAAAT
    GTCGTCAAAAATGGTGAACAGTCTGCTTATAAGGATTGGTTCCATATTCAACAATTCCCA
    GTGACAACTGAAAAGCTAGTTAATAAGAGAGACTTACCCTATCATGTTTTTGGTTTCGAG
    GACTATATGCCTAAGCTAAATACAGCCAATCCAGAGGTCAAGAATTATCTTTTAAAGGTT
    GCGACTTATTGGATTGAAGAGTTTAATATCGATGCTTGGCGTTTGGATGTGGCTAATGAG
    ATTGACCATCAGTTCTGGAAGGATTTTCGTAAGGCAGTTTTAGCTAAAAATCCTGATCTT
    TATATCCTAGGAGAAGTCTGGCATACATCTCAGCCTTGGCTAAATGGAGATGAGTTCCAT
    GCCGTCATGAATTATCCTTTATCTGATAGTATCAAGGACTATTTCTTACGAGGAATTAAG
    AAGACAGACCAGTTCATCGATGAAATCAATGGAGAGTCTATGTATTACAAGCAGCAGATT
    TCAGAGGTCATGTTTAATCTCTTGGATTCACATGATACAGAGCGAATCCTGTGGACGGCC
    AATGAAGATGTTCAACTGGTTAAATCAGCCTTAGCCTTTCTCTTTTTACAAAAAGGAACA
    CCGTGCATTTATTACGGAACCGAGCTAGCCTTGACTGGAGGACCAGATCCAGATTGTCGT
    CGTTGTATGCCTTGGGAACGTGTATCAAGTGACAATGATATGCTGAACTTTATGAAGAGG
    CTGATTAAGATTCGGAAATACGCGTCAGTAATCATTTCGCATGGCAAGTATAGCCTTCAA
    GAAATCAAATCTGATCTAGTAGCTCTGGAATGGAAATACGAAGGACGGATCCTCAAAGCA
    ATATTCAACCAATCAACAGAAGATTATCTTTTAGAGAAAGAAGCAGTAGCACTAGCAAGC
    AATTGCCAAGAATTGGAGAATCAGCTTGTCATCTCTCCAGATGGATTTGTGATTTTCTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1320
    1380
    1440
    1500
    1560
    1620
    1680
    1740

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SPH_1147 [new locus tag: SPH_RS05565 ]
  • symbol: SPH_1147
  • description: neopullulanase
  • length: 579
  • theoretical pI: 4.83919
  • theoretical MW: 67638
  • GRAVY: -0.416408

⊟Function[edit | edit source]

  • reaction:
    EC 3.2.1.135?  ExPASy
    Neopullulanase Hydrolysis of pullulan to panose (6-alpha-D-glucosylmaltose)
  • TIGRFAM:
    alpha,alpha-phosphotrehalase (TIGR02403; EC 3.2.1.93; HMM-score: 112.5)
    Metabolism Energy metabolism Biosynthesis and degradation of polysaccharides trehalose synthase (TIGR02456; EC 5.4.99.16; HMM-score: 93.6)
    and 8 more
    Metabolism Energy metabolism Biosynthesis and degradation of polysaccharides malto-oligosyltrehalose synthase (TIGR02401; EC 5.4.99.15; HMM-score: 89.5)
    Metabolism Energy metabolism Biosynthesis and degradation of polysaccharides malto-oligosyltrehalose trehalohydrolase (TIGR02402; EC 3.2.1.141; HMM-score: 86.6)
    pullulanase, type I (TIGR02104; EC 3.2.1.41; HMM-score: 60.6)
    Metabolism Energy metabolism Biosynthesis and degradation of polysaccharides glycogen debranching enzyme GlgX (TIGR02100; EC 3.2.1.-; HMM-score: 51.8)
    Metabolism Energy metabolism Biosynthesis and degradation of polysaccharides 1,4-alpha-glucan branching enzyme (TIGR01515; EC 2.4.1.18; HMM-score: 48)
    sucrose phosphorylase (TIGR03852; EC 2.4.1.7; HMM-score: 40.2)
    pullulanase, extracellular (TIGR02102; HMM-score: 38.4)
    Metabolism Energy metabolism Biosynthesis and degradation of polysaccharides alpha-1,6-glucosidases, pullulanase-type (TIGR02103; EC 3.2.1.41; HMM-score: 20.6)
  • TheSEED  :
    • Neopullulanase (EC 3.2.1.135)
    Carbohydrates Di- and oligosaccharides Maltose and Maltodextrin Utilization  Neopullulanase (EC 3.2.1.135)
  • PFAM:
    Glyco_hydro_tim (CL0058) Alpha-amylase; Alpha amylase, catalytic domain (PF00128; HMM-score: 250.3)
    and 4 more
    E-set (CL0159) Alpha-amylase_N; Alpha amylase, N-terminal ig-like domain (PF02903; HMM-score: 129.3)
    Glyco_hydro_tim (CL0058) DUF5696; Family of unknown function (DUF5696) (PF18952; HMM-score: 16.9)
    GHL6; Hypothetical glycosyl hydrolase 6 (PF14871; HMM-score: 13.3)
    PUP (CL0408) tRNA_deacylase; D-aminoacyl-tRNA deacylase (PF04414; HMM-score: 11.9)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 9.97
    • Cytoplasmic Membrane Score: 0
    • Cellwall Score: 0.01
    • Extracellular Score: 0.02
    • Internal Helices: 0
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.003504
    • TAT(Tat/SPI): 0.000095
    • LIPO(Sec/SPII): 0.000308
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MELSAIYHRPESEYAYLYKDKKLHIRIRTKKGDIESINLHYGDPFIFMEEFYQDTKEMVKITSGTLFDHWQVEVSVDFAHIQYLFEIRDTEGQNILYGDKGCVENSLENLHAIGNGFKLPYLHEIDACKVPDWVSNTVWYQIFPERFANGNALLNPEGTLDWDSSVTPKSDDFFGGDLQGIIDHMDYLQDLGITGLYLCPIFESTSNHKYNTTDYFEIDRHFGDKETFRELVDQAHHRGMKVMLDAVFNHIGSQSLQWKNVVKNGEQSAYKDWFHIQQFPVTTEKLVNKRDLPYHVFGFEDYMPKLNTANPEVKNYLLKVATYWIEEFNIDAWRLDVANEIDHQFWKDFRKAVLAKNPDLYILGEVWHTSQPWLNGDEFHAVMNYPLSDSIKDYFLRGIKKTDQFIDEINGESMYYKQQISEVMFNLLDSHDTERILWTANEDVQLVKSALAFLFLQKGTPCIYYGTELALTGGPDPDCRRCMPWERVSSDNDMLNFMKRLIKIRKYASVIISHGKYSLQEIKSDLVALEWKYEGRILKAIFNQSTEDYLLEKEAVALASNCQELENQLVISPDGFVIF

⊟Experimental data[edit | edit source]

  • protein localization:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator: CcpA regulon
    CcpA(TF)important in Global catabolite repression; RegPrecise 

⊟Expression data[edit | edit source]

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

⊟Relevant publications[edit | edit source]

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