From PneumoWiki
Jump to navigation Jump to search
PangenomeTIGR4
serotype 4
D39
serotype 2
D39V
serotype 2
Hungary19A-6
serotype 19A
EF3030
serotype 19F
670-6B
serotype 6B
6A-10
serotype 6A
70585
serotype 5
A026
serotype 19F
A66
serotype 3
AP200
serotype 11A
ASP0581
serotype 12F
ATCC 49619
serotype 19F
ATCC 700669
serotype 23F
BVJ1JL
serotype 1
CGSP14
serotype 14
G54
serotype 19F
HU-OH
serotype 3
Hu15
serotype 19A
Hu17
serotype 19A
INV104
serotype 1
INV200
serotype 14
JJA
serotype 14
MDRSPN001
serotype 19F
NCTC7466
serotype 2
NU83127
serotype 4
OXC141
serotype 3
P1031
serotype 1
R6
serotype 2
SP49
serotype 19A
SPN032672
serotype 1
SPN034156
serotype 3
SPN034183
serotype 3
SPN994038
serotype 3
SPN994039
serotype 3
SPNA45
serotype 3
ST556
serotype 19F
TCH8431/19A
serotype 19A
Taiwan19F-14
serotype 19F
Xen35
serotype 4
gamPNI0373
serotype 1

NCBI: 31-JAN-2014

⊟Summary[edit | edit source]

  • organism: Streptococcus pneumoniae Hungary19A-6
  • locus tag: SPH_1385 [new locus tag: SPH_RS06700 ]
  • pan locus tag?: PNEUPAN002555000
  • symbol: SPH_1385
  • pan gene symbol?: licA
  • synonym:
  • product: choline kinase

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SPH_1385 [new locus tag: SPH_RS06700 ]
  • symbol: SPH_1385
  • product: choline kinase
  • replicon: chromosome
  • strand: -
  • coordinates: 1283517..1284386
  • length: 870
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    GTGGAGAAAATCATTAAAGAAAAAATTTCTTCCTTACTTAGTCAAGAAGAGGAAGTCCTC
    AGTGTTGAACAACTGGGTGGAATGACCAATCAAAACTATTTGGCCAAAACAACAAATAAG
    CAATACATTGTTAAATTCTTTGGTAAAGGGACAGAAAAGCTTATCAATCGACAAGATGAA
    AAGTACAATCTTGAACTACTAAAGGATTTAGGCTTAGATGTAAAAAATTATCTTTTTGAT
    ATTGAAGCTGGTATCAAAGTAAATGAGTATATCGAATCTGCGATTACGCTTGATTCAACG
    TCAATCAAGACCAAGTTCGACAAAATTGCTCCAATATTACAAACTATTCATACGTCTGCT
    AAGGAATTAAGAGGAGAATTTGCTCCTTTTGAAGAAATCAAAAAATACGAATCCTTGATT
    GAAGAACAAATTCCTTATGCCAACTATGAATCTGTTAGAAATGCAGTCTTCTCCTTAGAG
    AAAAGACTGGCTGACTTAGGTGTTGACAGAAAATCTTGTCATATCGATTTGGTGCCTGAA
    AACTTTATCGAATCACCTCAAGGACGACTTTATTTGATTGACTGGGAATATTCATCAATG
    AATGATCCAATGTGGGATTTGGCTGCCCTCTTTTTAGAGTCTGAATTCACTTCCCAAGAG
    GAAGAAACTTTCTTATCTCACTATGAGGGTGACCAAACACCGGTTTCTCATGAAAAGATT
    GCTATTTATAAAATTTTACAAGATACTATTTGGAGTCTATGGACTGTCTATAAGGAAGAG
    CAAGGTGAAGATTTTGGTGACTATGGTGTGAATCGTTACCAAAGAGCTGTTAAAGGTTTG
    GCTTCTTATGGAGGTTCAGATGAAAAGTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    870

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SPH_1385 [new locus tag: SPH_RS06700 ]
  • symbol: SPH_1385
  • description: choline kinase
  • length: 289
  • theoretical pI: 4.43493
  • theoretical MW: 33372.3
  • GRAVY: -0.512111

⊟Function[edit | edit source]

  • TIGRFAM:
    Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Thiamine thiamine kinase (TIGR02721; EC 2.7.1.89; HMM-score: 70)
    and 3 more
    fibrobacter succinogenes paralogous family TIGR02172 (TIGR02172; HMM-score: 23)
    Metabolism Amino acid biosynthesis Aspartate family homoserine kinase (TIGR00938; EC 2.7.1.39; HMM-score: 21.7)
    Cellular processes Cellular processes Sporulation and germination spore coat protein YsxE (TIGR02904; HMM-score: 15.7)
  • TheSEED  :
    • Choline kinase (EC 2.7.1.32)
    Cell Wall and Capsule Capsular and extracellular polysacchrides Phosphorylcholine incorporation in LPS  Choline kinase (EC 2.7.1.32)
  • PFAM:
    PKinase (CL0016) Choline_kinase; Choline/ethanolamine kinase (PF01633; HMM-score: 126.2)
    and 2 more
    APH; Phosphotransferase enzyme family (PF01636; HMM-score: 47.7)
    DUF1679; Uncharacterized oxidoreductase dhs-27 (PF07914; HMM-score: 13.6)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.003663
    • TAT(Tat/SPI): 0.000267
    • LIPO(Sec/SPII): 0.000537
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MEKIIKEKISSLLSQEEEVLSVEQLGGMTNQNYLAKTTNKQYIVKFFGKGTEKLINRQDEKYNLELLKDLGLDVKNYLFDIEAGIKVNEYIESAITLDSTSIKTKFDKIAPILQTIHTSAKELRGEFAPFEEIKKYESLIEEQIPYANYESVRNAVFSLEKRLADLGVDRKSCHIDLVPENFIESPQGRLYLIDWEYSSMNDPMWDLAALFLESEFTSQEEETFLSHYEGDQTPVSHEKIAIYKILQDTIWSLWTVYKEEQGEDFGDYGVNRYQRAVKGLASYGGSDEK

⊟Experimental data[edit | edit source]

  • protein localization:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator:

⊟Expression data[edit | edit source]

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

⊟Relevant publications[edit | edit source]

Lei Wang, Yong-Liang Jiang, Jing-Ren Zhang, Cong-Zhao Zhou, Yuxing Chen
Structural and enzymatic characterization of the choline kinase LicA from Streptococcus pneumoniae.
PLoS One: 2015, 10(3);e0120467
[PubMed:25781969] [WorldCat.org] [DOI] (I e)

Retrieved from "http://pneumowiki.med.uni-greifswald.de/index.php?title=SPH_1385&oldid=12311"