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PangenomeTIGR4
serotype 4
D39
serotype 2
D39V
serotype 2
Hungary19A-6
serotype 19A
EF3030
serotype 19F
670-6B
serotype 6B
6A-10
serotype 6A
70585
serotype 5
A026
serotype 19F
A66
serotype 3
AP200
serotype 11A
ASP0581
serotype 12F
ATCC 49619
serotype 19F
ATCC 700669
serotype 23F
BVJ1JL
serotype 1
CGSP14
serotype 14
G54
serotype 19F
HU-OH
serotype 3
Hu15
serotype 19A
Hu17
serotype 19A
INV104
serotype 1
INV200
serotype 14
JJA
serotype 14
MDRSPN001
serotype 19F
NCTC7466
serotype 2
NU83127
serotype 4
OXC141
serotype 3
P1031
serotype 1
R6
serotype 2
SP49
serotype 19A
SPN032672
serotype 1
SPN034156
serotype 3
SPN034183
serotype 3
SPN994038
serotype 3
SPN994039
serotype 3
SPNA45
serotype 3
ST556
serotype 19F
TCH8431/19A
serotype 19A
Taiwan19F-14
serotype 19F
Xen35
serotype 4
gamPNI0373
serotype 1

NCBI: 31-JAN-2014

Summary[edit | edit source]

  • organism: Streptococcus pneumoniae Hungary19A-6
  • locus tag: SPH_1696 [new locus tag: SPH_RS08235 ]
  • pan locus tag?: PNEUPAN002966000
  • symbol: SPH_1696
  • pan gene symbol?: entB
  • synonym:
  • product: isochorismatase

Genome View[edit | edit source]

Gene[edit | edit source]

General[edit | edit source]

  • type: CDS
  • locus tag: SPH_1696 [new locus tag: SPH_RS08235 ]
  • symbol: SPH_1696
  • product: isochorismatase
  • replicon: chromosome
  • strand: -
  • coordinates: 1563675..1564250
  • length: 576
  • essential: unknown

Accession numbers[edit | edit source]

Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    ATGACAAAGGCTTTAATTTCGATTGATTATACAGAAGATTTTGTTGCTGATAGTGGGAAG
    CTGACAGCAGGTGCTCCAGCTCAGGCGATTTCGGATGCCATCAGCAAGGTAACTCGATTA
    GCTTTTGAACGAGGAGATTATATCTTCTTTACTATTGATGCTCATGAAGAAAACGATTGT
    TTCCATCCGGAAAGTAAGTTATTTCCTCCTCATAATCTGATTGGGACGAGTGGACGGAAT
    TTATATGGAGATTTGGGGATCTTTTATCAAGAGCATGGTTCAGACAGTCGTGTCTTTTGG
    ATGGATAAACGCCATTACTCAGCTTTTTCAGGGACTGACCTAGATATTCGTTTGAGAGAG
    CGTAGAGTGTCTACCGTTATCTTAACAGGTGTCTTGACGGATATCTGTGTCCTACATACA
    GCTATAGATGCCTATAATCTAGGATATGACATCGAGATTGTTAAACCAGCTGTTGCTTCC
    ATCTGGCCTGAAAATCATCAATTTGCCCTAGGTCATTTCAAAAATACACTCGGAGCTAAG
    TTAGTAGATGAAAATCTAAATGAACTTTCTGAGTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    576

Protein[edit | edit source]

General[edit | edit source]

  • locus tag: SPH_1696 [new locus tag: SPH_RS08235 ]
  • symbol: SPH_1696
  • description: isochorismatase
  • length: 191
  • theoretical pI: 4.8923
  • theoretical MW: 21368.8
  • GRAVY: -0.167539

Function[edit | edit source]

  • TIGRFAM:
    pyrimidine utilization protein B (TIGR03614; HMM-score: 30.7)
  • TheSEED  :
    • Nicotinamidase (EC 3.5.1.19)
    Cofactors, Vitamins, Prosthetic Groups, Pigments NAD and NADP NAD and NADP cofactor biosynthesis global  Nicotinamidase (EC 3.5.1.19)
  • PFAM:
    no clan defined Isochorismatase; Isochorismatase family (PF00857; HMM-score: 110.9)

Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.0086
    • TAT(Tat/SPI): 0.001801
    • LIPO(Sec/SPII): 0.000968
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit | edit source]

Protein sequence[edit | edit source]

  • MTKALISIDYTEDFVADSGKLTAGAPAQAISDAISKVTRLAFERGDYIFFTIDAHEENDCFHPESKLFPPHNLIGTSGRNLYGDLGIFYQEHGSDSRVFWMDKRHYSAFSGTDLDIRLRERRVSTVILTGVLTDICVLHTAIDAYNLGYDIEIVKPAVASIWPENHQFALGHFKNTLGAKLVDENLNELSE

Experimental data[edit | edit source]

  • protein localization:
  • interaction partners:

Expression & Regulation[edit | edit source]

Regulation[edit | edit source]

  • regulators: CcpA regulon, CodY (activation (repression)) regulon
    CcpA(TF)important in Global catabolite repression; RegPrecise    transcription unit transferred from TIGR4 data RegPrecise 
    CodY(TF)important in Amino acid metabolism; RegPrecise    transcription unit transferred from TIGR4 data RegPrecise 

Expression data[edit | edit source]

Biological Material[edit | edit source]

Mutants[edit | edit source]

Expression vector[edit | edit source]

lacZ fusion[edit | edit source]

GFP fusion[edit | edit source]

two-hybrid system[edit | edit source]

FLAG-tag construct[edit | edit source]

Antibody[edit | edit source]

Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

Literature[edit | edit source]

References[edit | edit source]

Relevant publications[edit | edit source]