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PangenomeTIGR4
serotype 4
D39
serotype 2
D39V
serotype 2
Hungary19A-6
serotype 19A
EF3030
serotype 19F
670-6B
serotype 6B
6A-10
serotype 6A
70585
serotype 5
A026
serotype 19F
A66
serotype 3
AP200
serotype 11A
ASP0581
serotype 12F
ATCC 49619
serotype 19F
ATCC 700669
serotype 23F
BVJ1JL
serotype 1
CGSP14
serotype 14
G54
serotype 19F
HU-OH
serotype 3
Hu15
serotype 19A
Hu17
serotype 19A
INV104
serotype 1
INV200
serotype 14
JJA
serotype 14
MDRSPN001
serotype 19F
NCTC7466
serotype 2
NU83127
serotype 4
OXC141
serotype 3
P1031
serotype 1
R6
serotype 2
SP49
serotype 19A
SPN032672
serotype 1
SPN034156
serotype 3
SPN034183
serotype 3
SPN994038
serotype 3
SPN994039
serotype 3
SPNA45
serotype 3
ST556
serotype 19F
TCH8431/19A
serotype 19A
Taiwan19F-14
serotype 19F
Xen35
serotype 4
gamPNI0373
serotype 1

NCBI: 10-MAR-2021

⊟Summary[edit | edit source]

  • organism: Streptococcus pneumoniae Hungary19A-6
  • locus tag: SPH_RS11280 [old locus tag: SPH_2301 ]
  • pan locus tag?: PNEUPAN003710000
  • symbol: SPH_RS11280
  • pan gene symbol?: malR
  • synonym:
  • product: LacI family DNA-binding transcriptional regulator

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SPH_RS11280 [old locus tag: SPH_2301 ]
  • symbol: SPH_RS11280
  • product: LacI family DNA-binding transcriptional regulator
  • replicon: chromosome
  • strand: +
  • coordinates: 2110601..2111587
  • length: 987
  • essential: unknown

⊟Accession numbers[edit | edit source]

  • Location: NC_010380 (2110601..2111587) NCBI
  • BioCyc: SPH_RS11280 BioCyc
  • MicrobesOnline: see SPH_2301
  • PneumoBrowse for strain D39V: SPV_1938 PneumoBrowse

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    ATGCCCGTTACGATTAAAGACGTGGCCAAGGCTGCTGGTGTTTCACCTTCAACCGTAACC
    CGTGTTATTCAAAATAAATCAACCATTAGCGACGAAACAAAAAAACGCGTTCGCAAGGCT
    ATGAAGGAGCTCAACTACCACCCAAACCTCAACGCTCGTAGCTTGGTAAGCAGTTATACG
    CAAGTTATCGGCTTAGTTCTTCCTGATGACTCAGATGCCTTCTACCAGAATCCTTTCTTT
    CCATCGGTTCTACGTGGCATCTCTCAAGTCGCATCTGAAAACCACTATGCCATTCAGATA
    GCAACAGGGAAAGATGAGAAGGAGCGTCTCAACGCTATTTCACAAATGGTCTACGGCAAG
    CGTGTAGATGGGCTAATCTTTCTCTATGCCCAAGAAGAAGATCCCCTCGTCAAACTCGTA
    GCAGAAGAACAGTTCCCCTTCCTTATCTTAGGTAAATCTCTATCTCCTTTCATCCCACTT
    GTCGATAATGACAACGTTCAAGCTGGTTTTGATGCGACAGAATATTTCATCAAAAAAGGC
    TGCAAACGCATTGCCTTTATCGGAGGAAGTAAAAAGCTCTTCGTGACCAAAGATCGTTTA
    ACAGGCTATGAACAGGCGCTGAAACATTACAAACTTACCACTGACAACAATCGCATCTAC
    TTTGCAGACGAGTTTCTGGAAGAAAAGGGCTATAAATTTAGCAAGCGATTATTCAAGCAC
    GATCCACAAATTGATGCCATCATCACAACCGATAGCCTCCTAGCTGAAGGAGTCTGTGAC
    TATATTGCCAAACACCAGCTGGATGTCCCTGTTCTCAGCTTTGACTCGGTTAATCCCAAG
    CTCAATTTGGCAGCCTATATAGATATCAATAGCCTAGCCCTTGGACGCACTTCTTTAGAA
    ACTATCCTCTGCATCATCAATGATTCTAAAGAAAATAGACAAATCTGCTACCGTCAGTTG
    ATTGCCCACAAAATTATTGAAAAATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    987

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SPH_RS11280 [old locus tag: SPH_2301 ]
  • symbol: SPH_RS11280
  • description: LacI family DNA-binding transcriptional regulator
  • length: 328
  • theoretical pI: 8.53105
  • theoretical MW: 36993.3
  • GRAVY: -0.214634

⊟Function[edit | edit source]

  • TIGRFAM:
    Signal transduction Regulatory functions DNA interactions catabolite control protein A (TIGR01481; HMM-score: 148)
    Signal transduction Regulatory functions DNA interactions D-fructose-responsive transcription factor (TIGR02417; HMM-score: 136.3)
    and 6 more
    Signal transduction Regulatory functions DNA interactions trehalose operon repressor (TIGR02405; HMM-score: 59.6)
    Genetic information processing Mobile and extrachromosomal element functions Other addiction module antidote protein, HigA family (TIGR02607; HMM-score: 20.2)
    Signal transduction Regulatory functions DNA interactions addiction module antidote protein, HigA family (TIGR02607; HMM-score: 20.2)
    Signal transduction Regulatory functions Protein interactions addiction module antidote protein, HigA family (TIGR02607; HMM-score: 20.2)
    Genetic information processing Transcription Transcription factors RNA polymerase sigma-54 factor (TIGR02395; HMM-score: 15.1)
    Signal transduction Regulatory functions DNA interactions mycofactocin system transcriptional regulator (TIGR03968; HMM-score: 13)
  • TheSEED: see SPH_2301
  • PFAM:
    HTH (CL0123) LacI; Bacterial regulatory proteins, lacI family (PF00356; HMM-score: 78.9)
    Periplas_BP (CL0144) Peripla_BP_1; Periplasmic binding proteins and sugar binding domain of LacI family (PF00532; HMM-score: 73.2)
    and 21 more
    Peripla_BP_3; Periplasmic binding protein-like domain (PF13377; HMM-score: 47.3)
    Peripla_BP_4; Periplasmic binding protein domain (PF13407; HMM-score: 40.4)
    HTH (CL0123) HTH_3; Helix-turn-helix (PF01381; HMM-score: 21.8)
    HTH_23; Homeodomain-like domain (PF13384; HMM-score: 16.6)
    MerR; MerR family regulatory protein (PF00376; HMM-score: 15.9)
    Sigma54_DBD; Sigma-54, DNA binding domain (PF04552; HMM-score: 15.6)
    HTH_Tnp_ISL3; Helix-turn-helix domain of transposase family ISL3 (PF13542; HMM-score: 15)
    TetR_N; Bacterial regulatory proteins, tetR family (PF00440; HMM-score: 14.6)
    HTH_6; Helix-turn-helix domain, rpiR family (PF01418; HMM-score: 14.6)
    HTH_38; Helix-turn-helix domain (PF13936; HMM-score: 14.6)
    no clan defined TnsD; Tn7-like transposition protein D (PF15978; HMM-score: 14.4)
    HTH (CL0123) HTH_31; Helix-turn-helix domain (PF13560; HMM-score: 14.3)
    HTH_24; Winged helix-turn-helix DNA-binding (PF13412; HMM-score: 13.9)
    HTH_AraC; Bacterial regulatory helix-turn-helix proteins, AraC family (PF00165; HMM-score: 13.8)
    MarR; MarR family (PF01047; HMM-score: 13.4)
    HTH_7; Helix-turn-helix domain of resolvase (PF02796; HMM-score: 13.4)
    HTH_10; HTH DNA binding domain (PF04967; HMM-score: 12.9)
    MarR_2; MarR family (PF12802; HMM-score: 12.7)
    HTH_17; Helix-turn-helix domain (PF12728; HMM-score: 12.6)
    HTH_Crp_2; Crp-like helix-turn-helix domain (PF13545; HMM-score: 12)
    HTH_IclR; IclR helix-turn-helix domain (PF09339; HMM-score: 11.9)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:
  • genes regulated by MalR*, TF important in Maltose utilization, Maltodextrin utilization: see SPH_2301

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 9.97
    • Cytoplasmic Membrane Score: 0
    • Cellwall Score: 0.01
    • Extracellular Score: 0.02
    • Internal Helices: 0
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.009223
    • TAT(Tat/SPI): 0.003126
    • LIPO(Sec/SPII): 0.001185
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MPVTIKDVAKAAGVSPSTVTRVIQNKSTISDETKKRVRKAMKELNYHPNLNARSLVSSYTQVIGLVLPDDSDAFYQNPFFPSVLRGISQVASENHYAIQIATGKDEKERLNAISQMVYGKRVDGLIFLYAQEEDPLVKLVAEEQFPFLILGKSLSPFIPLVDNDNVQAGFDATEYFIKKGCKRIAFIGGSKKLFVTKDRLTGYEQALKHYKLTTDNNRIYFADEFLEEKGYKFSKRLFKHDPQIDAIITTDSLLAEGVCDYIAKHQLDVPVLSFDSVNPKLNLAAYIDINSLALGRTSLETILCIINDSKENRQICYRQLIAHKIIEK

⊟Experimental data[edit | edit source]

  • protein localization:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator:

⊟Expression data[edit | edit source]

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

⊟Relevant publications[edit | edit source]

C Nieto, A Puyet, M Espinosa
MalR-mediated regulation of the Streptococcus pneumoniae malMP operon at promoter PM. Influence of a proximal divergent promoter region and competition between MalR and RNA polymerase proteins.
J Biol Chem: 2001, 276(18);14946-54
[PubMed:11278784] [WorldCat.org] [DOI] (P p)Muhammad Afzal, Sulman Shafeeq, Irfan Manzoor, Oscar P Kuipers
Maltose-Dependent Transcriptional Regulation of the mal Regulon by MalR in Streptococcus pneumoniae.
PLoS One: 2015, 10(6);e0127579
[PubMed:26030923] [WorldCat.org] [DOI] (I e)

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