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JJA
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MDRSPN001
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NCTC7466
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OXC141
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SPN034183
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SPN994038
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SPN994039
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Xen35
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gamPNI0373
serotype 1

NCBI: 31-JAN-2014

⊟Summary[edit | edit source]

  • organism: Streptococcus pneumoniae JJA
  • locus tag: SPJ_0386 [new locus tag: SPJ_RS01960 ]
  • pan locus tag?: PNEUPAN001267000
  • symbol: SPJ_0386
  • pan gene symbol?: mtlD
  • synonym:
  • product: mannitol-1-phosphate 5-dehydrogenase

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SPJ_0386 [new locus tag: SPJ_RS01960 ]
  • symbol: SPJ_0386
  • product: mannitol-1-phosphate 5-dehydrogenase
  • replicon: chromosome
  • strand: +
  • coordinates: 370305..371441
  • length: 1137
  • essential: unknown

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    ATGAAACATTCTGTTCATTTTGGTGCCGGTAATATCGGTCGTGGTTTTATAGGTGAAATT
    CTATTTAAAAATGATTTCCATATTGATTTTGTGGATGTCAATAATCAGATAATTCATGCT
    CTAAATGAAAAGGGCAAGTATGAAATTGAAATTGCACAGAAAGGACAGTCTCGTATAGAA
    GTAACTAATGTGGCTGGAATTAATAGCAAAGAACATCCTGAGCAAGTCATTGAAGCGATT
    CAAAAGACGGATATTATTACTACTGCAATCGGACCTAATATACTCCCTTTTATCGCTGAA
    CTTCTAGCCAAAGGAATCGAAGCTCGCCGAGTTGCAGGAAATACACAGGCATTGGATGTT
    ATGGCCTGTGAAAATATGATTGGCGGGTCTCAATTTCTTTATCAAGAAGTCAAGAAATAT
    TTAAGTCCGGAAGGTTTGACATTTGCTGATAACTACATAGGTTTTCCAAATGCTGCAGTA
    GACAGGATTGTTCCAACACAAAGTCACGAAGATTCCCTTTTTGTTATGGTCGAGCCCTTT
    AATGAATGGGTCGTGGAAACCAAGCGTCTTAAAAATCCAGATTTACGTCTAGAAGATGTG
    CATTATGAAGAAGATTTAGAACCCTTTATTGAGCGAAAACTTTTTTCAGTCAATTCTGGA
    CATGCAACTTCAGCTTACATTGGTGCGCATTATGGTGCCAAGACAATTTTGGAAGCTCTT
    CAAAATCCTAATATCAAATCTCGGATTGAATCTGTATTAGCTGAAATTCGGAGTCTCTTG
    ATTGCCAAATGGAACTTTGATAAAAAAGAATTGGAGAATTATCACAAAGTCATTATAGAA
    CGATTCGAAAACCCTTTCATAGTGGACGAGGTTAGTCGCGTAGCTCGTACTCCAATCCGA
    AAATTAGGCTATAATGAACGATTCATCCGGCCGATACGTGAATTGAAAGAACTCAGTTTG
    TCATATAAAAACCTACTTAAAACAGTTGGCTATGCCTTTGACTATCGCGATGTAAATGAT
    GAAGAAAGTATTCGATTAGGTGAATTGTTGGCTAAACAATCAGTCAAAGATGTTGTTATA
    CAAGTTACAGGTTTAGACGACCAAGAATTGATTGAGCAAATTGTAGAGTATATTTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1137

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SPJ_0386 [new locus tag: SPJ_RS01960 ]
  • symbol: SPJ_0386
  • description: mannitol-1-phosphate 5-dehydrogenase
  • length: 378
  • theoretical pI: 5.13039
  • theoretical MW: 43057.8
  • GRAVY: -0.251058

⊟Function[edit | edit source]

  • reaction:
    EC 1.1.1.17?  ExPASy
    Mannitol-1-phosphate 5-dehydrogenase D-mannitol 1-phosphate + NAD+ = D-fructose 6-phosphate + NADH
  • TIGRFAM:
    Cellular processes Cellular processes Toxin production and resistance radical SAM P-methyltransferase, PhpK family (TIGR04479; EC 2.1.-.-; HMM-score: 11.9)
  • TheSEED  :
    • Mannitol-1-phosphate 5-dehydrogenase (EC 1.1.1.17)
    Carbohydrates Sugar alcohols Mannitol Utilization  Mannitol-1-phosphate 5-dehydrogenase (EC 1.1.1.17)
  • PFAM:
    6PGD_C (CL0106) Mannitol_dh_C; Mannitol dehydrogenase C-terminal domain (PF08125; HMM-score: 208.4)
    and 2 more
    NADP_Rossmann (CL0063) Mannitol_dh; Mannitol dehydrogenase Rossmann domain (PF01232; HMM-score: 98.5)
    no clan defined CofD; 2-phospho-L-lactate transferase CofD (PF01933; HMM-score: 18.6)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 9.97
    • Cytoplasmic Membrane Score: 0
    • Cellwall Score: 0.01
    • Extracellular Score: 0.02
    • Internal Helices: 0
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.003751
    • TAT(Tat/SPI): 0.000154
    • LIPO(Sec/SPII): 0.000352
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MKHSVHFGAGNIGRGFIGEILFKNDFHIDFVDVNNQIIHALNEKGKYEIEIAQKGQSRIEVTNVAGINSKEHPEQVIEAIQKTDIITTAIGPNILPFIAELLAKGIEARRVAGNTQALDVMACENMIGGSQFLYQEVKKYLSPEGLTFADNYIGFPNAAVDRIVPTQSHEDSLFVMVEPFNEWVVETKRLKNPDLRLEDVHYEEDLEPFIERKLFSVNSGHATSAYIGAHYGAKTILEALQNPNIKSRIESVLAEIRSLLIAKWNFDKKELENYHKVIIERFENPFIVDEVSRVARTPIRKLGYNERFIRPIRELKELSLSYKNLLKTVGYAFDYRDVNDEESIRLGELLAKQSVKDVVIQVTGLDDQELIEQIVEYI

⊟Experimental data[edit | edit source]

  • protein localization:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulators: MtlR* (activation) regulon, CcpA regulon
    MtlR*(TF)important in Mannitol utilization; RegPrecise    transcription unit transferred from TIGR4 data RegPrecise 
    CcpA(TF)important in Global catabolite repression; RegPrecise    transcription unit transferred from TIGR4 data RegPrecise 

⊟Expression data[edit | edit source]

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

⊟Relevant publications[edit | edit source]

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