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Hu15
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Hu17
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INV200
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JJA
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MDRSPN001
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NCTC7466
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NU83127
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OXC141
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SPN032672
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SPN034156
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SPN034183
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SPN994039
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SPNA45
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ST556
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TCH8431/19A
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Xen35
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serotype 1

NCBI: 31-JAN-2014

⊟Summary[edit | edit source]

  • organism: Streptococcus pneumoniae JJA
  • locus tag: SPJ_1006 [new locus tag: SPJ_RS05030 ]
  • pan locus tag?: PNEUPAN002214000
  • symbol: ppc
  • pan gene symbol?: ppc
  • synonym:
  • product: phosphoenolpyruvate carboxylase

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SPJ_1006 [new locus tag: SPJ_RS05030 ]
  • symbol: ppc
  • product: phosphoenolpyruvate carboxylase
  • replicon: chromosome
  • strand: +
  • coordinates: 955983..958679
  • length: 2697
  • essential: unknown

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    ATGTCTCTTCAAAAATTAGAAAATTATAGTAATAAAAGTGTTGTGCAAGAAGAAGTCTTG
    ATTCTAACAGAATTACTGGAAGATATTACTAAAAATATGCTTGCCCCAGAGACCTTTGAA
    AAAATAATACAGTTGAAAGAATTATCAACGCAGGAAGATTATCAAGGTCTAAACCGTCTA
    GTGACTAGCTTATCAAATGATGAAATGGTCTATATTTCACGCTATTTCTCTATCTTACCT
    CTTTTGATCAATATTTCAGAGGATGTGGATTTAGCTTATGAAATCAATCATCAAAATAAT
    ATTGATCAGGACTATTTAGGTAAATTATCTACAACGATTAAATTGGTAGCAGAAAAGGAA
    AATGCCGTTGAGATCCTAGAACACTTGAATGTTGTCCCTGTTTTGACAGCCCATCCAACA
    CAAGTGCAACGCAAAAGTATGTTGGATTTAACAAATCATATTCATAGTCTTTTGCGTAAA
    TACCGTGATGTTAAGTTGGGGTTGATCAATAAAGATAAATGGTACAATGATTTGCGTCGT
    TACATCGAAATTATCATGCAGACAGACATGATTCGTGAGAAAAAATTAAAAGTGACTAAC
    GAAATCACGAATGCTATGGAATATTATAACAGCTCCTTTTTGAAAGCTGTACCTCATTTG
    ACGACGGAGTATAAGCGCTTAGCGCAAGCGCATGGTCTGAATTTAAAACAGGCTAAACCA
    ATCACCATGGGTATGTGGATAGGTGGTGACCGTGATGGAAATCCATTTGTTACAGCAGAG
    ACCTTGAAGCAGTCTGCACTCACTCAATGTGAAGTCATCATGAACTACTATGATAAAAAG
    ATTTACCAACTTTATCGTGAATTTTCTCTTTCAACTAGCATTGTCAACGTCAGCAAGCAA
    GTCAGAGAAATGGCTCGTCAATCCAAGGATAACTCGATTTACCGCGAAAAAGAGCTTTAC
    CGTCGTGCCTTGTTTGATATTCAATCAAAAATTCAGGCAACTAAAACCTATCTGATTGAG
    GATGAAGAAGTTGGGACTCGTTATGAAACCGCCAATGATTTCTACAAGGATTTGATTGCC
    ATTCGAGATTCTCTACTAGAAAATAAGGGTGAGTCCTTGATTTCAGGTGATTTTGTGGAA
    TTATTGCAGGCAGTAGAGATATTTGGTTTTTACCTAGCATCAATTGATATGCGACAAGAC
    TCTAGCGTCTATGAAGCCTGTGTGGCAGAACTCTTGAAATCAGCAGGAATTCATTCTCGT
    TATAGCGAGTTGAGCGAAGAAGAAAAGTGTGACCTTCTCTTGAAAGAATTAGAAGAAGAT
    CCCCGAATTCTTTCTGCGACTCACGCAGAAAAATCAGAATTATTAGCAAAAGAATTAGCT
    ATTTTTAAGACGGCTCGTGTTTTGAAAGATAAGTTGGGAGATGATGTCATCCGTCAGACC
    ATCATTTCACATGCAACCAGCCTTTCTGATATGCTAGAATTAGCTATTCTGTTAAAAGAA
    GTAGGACTGGTGGATACGGAAAGGGCGCGTGTTCAGATTGTTCCCCTTTTTGAAACAATT
    GAAGACTTGGATCATTCAGAGGAAACAATGAGAAAATATCTTTCTCTTAGCCTTGCCAAA
    AAATGGATTGACTCACGAAATAACTACCAAGAAATCATGCTTGGCTACTCTGACAGTAAT
    AAAGATGGCGGTTACTTGTCATCATGTTGGACCCTCTACAAGGCTCAACAACAATTGACT
    GCTATTGGAGATGAATTTGGCGTTAAGGTTACCTTCTTCCATGGTCGTGGTGGTACTGTC
    GGTCGTGGTGGTGGGCCAACCTATGAAGCCATTACATCTCAACCGCTCAAGTCTATCAAG
    GATCGTATCCGCTTGACGGAGCAGGGTGAAGTAATTGGGAATAAATACGGTAACAAAGAC
    GCCGCTTACTATAACCTTGAAATGCTAGTATCGGCAGCTATTAACCGTATGATTACTCAG
    AAGAAGAGCGATACCAATACCCCAAATCGTTATGAAGCCATTATGGATCAAGTAGTGGAC
    CGTAGTTACGATATCTACCGTGATTTGGTCTTTGGTAATGAGCATTTCTATGATTATTTC
    TTCGAGTCAAGTCCAATCAAGGCTATTTCAAGTTTTAATATTGGTTCTCGTCCAGCCGCT
    CGTAAGACTATTACTGAAATCGGTGGATTGCGTGCCATCCCTTGGGTATTCTCATGGTCA
    CAGAGTCGTGTTATGTTCCCTGGATGGTACGGGGTTGGTTCAAGCTTCAAGGAATTTATC
    AATAAAAATCCAGAGAATATTGCTATCTTACGAGATATGTACCAAAATTGGCCTTTCTTC
    CAATCGCTTCTTTCAAATGTTGATATGGTTTTGTCAAAATCAAATATGAATATTGCTTTT
    GAATATGCTAAACTTTGTGAAGACGAGCAAGTTAAGGCCATCTATGAGACTATTTTAAAT
    GAATGGCAAGTTACTAAGAACGTTATCTTGGCTATTGAAGGACATGACGAACTCTTAGCT
    GACAATCCATATCTAAAAGCTAGTCTGGATTACCGTATGCCTTACTTTAATATTCTCAAC
    TATATTCAGTTGGAGTTGATTAAACGCCAACGTCGTGGAGAATTGTCCAGTGATCAAGAA
    CGATTGATTCATATCACCATCAACGGAATTGCGACAGGATTGCGTAATTCAGGTTGA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1320
    1380
    1440
    1500
    1560
    1620
    1680
    1740
    1800
    1860
    1920
    1980
    2040
    2100
    2160
    2220
    2280
    2340
    2400
    2460
    2520
    2580
    2640
    2697

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SPJ_1006 [new locus tag: SPJ_RS05030 ]
  • symbol: Ppc
  • description: phosphoenolpyruvate carboxylase
  • length: 898
  • theoretical pI: 5.27728
  • theoretical MW: 103285
  • GRAVY: -0.352004

⊟Function[edit | edit source]

  • reaction:
    EC 4.1.1.31?  ExPASy
    Phosphoenolpyruvate carboxylase Phosphate + oxaloacetate = H2O + phosphoenolpyruvate + HCO3(-)
  • TIGRFAM:
    Metabolism Energy metabolism Other phosphoenolpyruvate carboxylase (TIGR02751; EC 4.1.1.31; HMM-score: 29.3)
  • TheSEED  :
    • Phosphoenolpyruvate carboxylase (EC 4.1.1.31)
    Carbohydrates Central carbohydrate metabolism Pyruvate metabolism I: anaplerotic reactions, PEP  Phosphoenolpyruvate carboxylase (EC 4.1.1.31)
    and 2 more
    Carbohydrates Fermentation Fermentations: Mixed acid  Phosphoenolpyruvate carboxylase (EC 4.1.1.31)
    Carbohydrates One-carbon Metabolism Serine-glyoxylate cycle  Phosphoenolpyruvate carboxylase (EC 4.1.1.31)
  • PFAM:
    PK_TIM (CL0151) PEPcase; Phosphoenolpyruvate carboxylase (PF00311; HMM-score: 903.8)
    and 3 more
    PEPcase_2; Phosphoenolpyruvate carboxylase (PF14010; HMM-score: 30.3)
    SLOG (CL0349) Lysine_decarbox; Possible lysine decarboxylase (PF03641; HMM-score: 13.1)
    HTH (CL0123) PaaX; PaaX-like protein (PF07848; HMM-score: 12.6)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.002664
    • TAT(Tat/SPI): 0.000079
    • LIPO(Sec/SPII): 0.000151
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MSLQKLENYSNKSVVQEEVLILTELLEDITKNMLAPETFEKIIQLKELSTQEDYQGLNRLVTSLSNDEMVYISRYFSILPLLINISEDVDLAYEINHQNNIDQDYLGKLSTTIKLVAEKENAVEILEHLNVVPVLTAHPTQVQRKSMLDLTNHIHSLLRKYRDVKLGLINKDKWYNDLRRYIEIIMQTDMIREKKLKVTNEITNAMEYYNSSFLKAVPHLTTEYKRLAQAHGLNLKQAKPITMGMWIGGDRDGNPFVTAETLKQSALTQCEVIMNYYDKKIYQLYREFSLSTSIVNVSKQVREMARQSKDNSIYREKELYRRALFDIQSKIQATKTYLIEDEEVGTRYETANDFYKDLIAIRDSLLENKGESLISGDFVELLQAVEIFGFYLASIDMRQDSSVYEACVAELLKSAGIHSRYSELSEEEKCDLLLKELEEDPRILSATHAEKSELLAKELAIFKTARVLKDKLGDDVIRQTIISHATSLSDMLELAILLKEVGLVDTERARVQIVPLFETIEDLDHSEETMRKYLSLSLAKKWIDSRNNYQEIMLGYSDSNKDGGYLSSCWTLYKAQQQLTAIGDEFGVKVTFFHGRGGTVGRGGGPTYEAITSQPLKSIKDRIRLTEQGEVIGNKYGNKDAAYYNLEMLVSAAINRMITQKKSDTNTPNRYEAIMDQVVDRSYDIYRDLVFGNEHFYDYFFESSPIKAISSFNIGSRPAARKTITEIGGLRAIPWVFSWSQSRVMFPGWYGVGSSFKEFINKNPENIAILRDMYQNWPFFQSLLSNVDMVLSKSNMNIAFEYAKLCEDEQVKAIYETILNEWQVTKNVILAIEGHDELLADNPYLKASLDYRMPYFNILNYIQLELIKRQRRGELSSDQERLIHITINGIATGLRNSG

⊟Experimental data[edit | edit source]

  • protein localization:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator: CodY (activation (repression)) regulon
    CodY(TF)important in Amino acid metabolism; RegPrecise 

⊟Expression data[edit | edit source]

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

⊟Relevant publications[edit | edit source]

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