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PangenomeTIGR4
serotype 4
D39
serotype 2
D39V
serotype 2
Hungary19A-6
serotype 19A
EF3030
serotype 19F
670-6B
serotype 6B
6A-10
serotype 6A
70585
serotype 5
A026
serotype 19F
A66
serotype 3
AP200
serotype 11A
ASP0581
serotype 12F
ATCC 49619
serotype 19F
ATCC 700669
serotype 23F
BVJ1JL
serotype 1
CGSP14
serotype 14
G54
serotype 19F
HU-OH
serotype 3
Hu15
serotype 19A
Hu17
serotype 19A
INV104
serotype 1
INV200
serotype 14
JJA
serotype 14
MDRSPN001
serotype 19F
NCTC7466
serotype 2
NU83127
serotype 4
OXC141
serotype 3
P1031
serotype 1
R6
serotype 2
SP49
serotype 19A
SPN032672
serotype 1
SPN034156
serotype 3
SPN034183
serotype 3
SPN994038
serotype 3
SPN994039
serotype 3
SPNA45
serotype 3
ST556
serotype 19F
TCH8431/19A
serotype 19A
Taiwan19F-14
serotype 19F
Xen35
serotype 4
gamPNI0373
serotype 1

NCBI: 31-JAN-2014

⊟Summary[edit | edit source]

  • organism: Streptococcus pneumoniae JJA
  • locus tag: SPJ_1057 [new locus tag: SPJ_RS05290 ]
  • pan locus tag?: PNEUPAN002267000
  • symbol: SPJ_1057
  • pan gene symbol?: gapN
  • synonym:
  • product: NADP-dependent glyceraldehyde-3-phosphate dehydrogenase

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SPJ_1057 [new locus tag: SPJ_RS05290 ]
  • symbol: SPJ_1057
  • product: NADP-dependent glyceraldehyde-3-phosphate dehydrogenase
  • replicon: chromosome
  • strand: +
  • coordinates: 1005216..1006640
  • length: 1425
  • essential: unknown

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    TTGACAAGATATCAGAATTTAGTAAATGGAAAATGGAAATCATCTGAACAAGAAATTACG
    ATTTATTCACCAATCAATCAAGAAGAATTGGGTACAGTTCCAGCCATGACTCAGACTGAA
    GCTGATGAGGCTATGCAAGCTGCGCGTGCAGCCCTGCCAGCATGGCGAGCTTTATCAGCA
    GTTGAACGTGCGGCTTATTTACATAAAACAGCAGCTATTTTAGAACGCGATAAGGAAGAA
    ATTGGTACTATCCTTGCCAAAGAAGTAGCAAAAGGGATTAAAGCAGCAATTGGAGAAGTA
    GTGCGTACAGCAGACTTGATTCGTTATGCTGCTGAGGAAGGTCTCCGTATCACTGGACAA
    GCAATGGAAGGTGGTGGTTTTGAGGCAGCAAGTAAAAACAAACTGGCTGTTGTCCGTCGT
    GAACCAGTTGGTATCGTGCTAGCGATTGCTCCCTTTAATTATCCAGTTAATTTATCTGCT
    TCTAAAATTGCACCTGCCTTGATTGCAGGGAATGTGGTCATGTTTAAGCCACCAACACAA
    GGTTCCATTTCTGGACTCTTGTTGGCTAAAGCATTTGAAGAAGCAGGGATTCCGGCAGGT
    GTTTTCAACACCATTACAGGTCGTGGTTCAGAAATTGGGGATTATATCATTGAGCACAAA
    GAAGTCAACTTCATCAACTTTACAGGTTCAACTCCTATTGGAGAACGTATTGGTCGTTTA
    GCTGGTATGCGTCCTATCATGTTGGAACTTGGTGGGAAAGATGCAGCTCTTGTACTAGAA
    GATGCAGATTTGGAACATGCTGCCAAGCAAATTGTTGCGGGAGCCTTTAGCTACTCAGGA
    CAACGTTGCACGGCCATTAAACGTGTCATTGTTCTCGAAAGTGTAGCAGATAAATTAGCT
    ACTTTGCTTCAGGAAGAAGTTTCTAAATTAACAGTTGGTGATCCATTTGACAATGCTGAT
    ATTACACCTGTTATTGACAATGCTTCAGCCGACTTCATTTGGGGCTTGATTGAGGATGCA
    CAAGAAAAAGAAGCTCAGGCTCTTACACCAATCAAACGTGAGGGCAATCTTCTCTGGCCA
    GTGCTTTTTGACCAAGTTACAAAAGATATGAAAGTGGCATGGGAAGAGCCATTTGGTCCT
    GTTTTACCAATCATTCGTGTGGCTAGTGTAGAGGAAGCTATTGCCTTTGCCAACGAATCT
    GAATTCGGCCTTCAATCATCAGTCTTTACAAATGATTTCAAAAAAGCCTTTGAAATTGCT
    GAAAAACTTGAAGTAGGTACAGTCCACATTAATAATAAAACCCAGCGTGGTCCAGATAAT
    TTCCCATTCCTTGGTGTCAAAGGTTCTGGAGCTGGAGTGCAAGGAATTAAATATAGCATT
    GAAGCGATGACAAATGTCAAATCCATTGTTTTTGATGTGAAATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1320
    1380
    1425

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SPJ_1057 [new locus tag: SPJ_RS05290 ]
  • symbol: SPJ_1057
  • description: NADP-dependent glyceraldehyde-3-phosphate dehydrogenase
  • length: 474
  • theoretical pI: 4.81415
  • theoretical MW: 51097.2
  • GRAVY: 0.00738397

⊟Function[edit | edit source]

  • reaction:
    EC 1.2.1.9?  ExPASy
    Glyceraldehyde-3-phosphate dehydrogenase (NADP+) D-glyceraldehyde 3-phosphate + NADP+ + H2O = 3-phospho-D-glycerate + NADPH
  • TIGRFAM:
    putative phosphonoacetaldehyde dehydrogenase (TIGR03250; EC 1.2.1.-; HMM-score: 353.9)
    Metabolism Central intermediary metabolism Other succinate-semialdehyde dehydrogenase (TIGR01780; EC 1.2.1.-; HMM-score: 332.5)
    5-carboxymethyl-2-hydroxymuconate semialdehyde dehydrogenase (TIGR02299; EC 1.2.1.60; HMM-score: 322.3)
    Cellular processes Cellular processes Adaptations to atypical conditions betaine-aldehyde dehydrogenase (TIGR01804; EC 1.2.1.8; HMM-score: 318.2)
    Metabolism Energy metabolism Amino acids and amines putative delta-1-pyrroline-5-carboxylate dehydrogenase (TIGR01237; EC 1.2.1.88; HMM-score: 300.3)
    and 11 more
    Metabolism Energy metabolism Other 2-hydroxymuconic semialdehyde dehydrogenase (TIGR03216; EC 1.2.1.-; HMM-score: 264.7)
    1-pyrroline dehydrogenase (TIGR03374; EC 1.2.1.19; HMM-score: 236.1)
    Metabolism Energy metabolism Amino acids and amines methylmalonate-semialdehyde dehydrogenase (acylating) (TIGR01722; EC 1.2.1.27; HMM-score: 231.7)
    Metabolism Energy metabolism Amino acids and amines succinylglutamate-semialdehyde dehydrogenase (TIGR03240; EC 1.2.1.71; HMM-score: 225.8)
    Metabolism Energy metabolism Amino acids and amines delta-1-pyrroline-5-carboxylate dehydrogenase (TIGR01238; EC 1.2.1.88; HMM-score: 215.3)
    Unknown function Enzymes of unknown specificity aldehyde dehydrogenase, Rv0768 family (TIGR04284; EC 1.2.1.-; HMM-score: 208.5)
    Metabolism Energy metabolism Amino acids and amines 1-pyrroline-5-carboxylate dehydrogenase (TIGR01236; EC 1.2.1.88; HMM-score: 170.9)
    phenylacetic acid degradation protein paaN (TIGR02278; HMM-score: 109.2)
    phenylacetic acid degradation protein paaN (TIGR02288; HMM-score: 38.2)
    acetaldehyde dehydrogenase (acetylating) (TIGR02518; EC 1.2.1.10; HMM-score: 32.6)
    Metabolism Amino acid biosynthesis Glutamate family glutamate-5-semialdehyde dehydrogenase (TIGR00407; EC 1.2.1.41; HMM-score: 24.4)
  • TheSEED  :
    • Glyceraldehyde-3-phosphate dehydrogenase (NADP(+)) (EC 1.2.1.9)
    Carbohydrates Central carbohydrate metabolism Entner-Doudoroff Pathway  Glyceraldehyde-3-phosphate dehydrogenase (NADP(+)) (EC 1.2.1.9)
    and 3 more
    Carbohydrates Central carbohydrate metabolism Entner-Doudoroff Pathway  Non-phosphorylating glyceraldehyde-3-phosphate dehydrogenase (NADP) (EC 1.2.1.9)
    Carbohydrates Central carbohydrate metabolism Glycolysis and Gluconeogenesis  Glyceraldehyde-3-phosphate dehydrogenase (NADP(+)) (EC 1.2.1.9)
    Carbohydrates Central carbohydrate metabolism Glycolysis and Gluconeogenesis  Non-phosphorylating glyceraldehyde-3-phosphate dehydrogenase (NADP) (EC 1.2.1.9)
  • PFAM:
    ALDH-like (CL0099) Aldedh; Aldehyde dehydrogenase family (PF00171; HMM-score: 516.4)
    and 1 more
    CDA (CL0109) SNAD3; Secreted Novel AID/APOBEC-like Deaminase 3 (PF18749; HMM-score: 13.7)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 9.97
    • Cytoplasmic Membrane Score: 0
    • Cellwall Score: 0.01
    • Extracellular Score: 0.02
    • Internal Helices: 0
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.009499
    • TAT(Tat/SPI): 0.001164
    • LIPO(Sec/SPII): 0.000821
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MTRYQNLVNGKWKSSEQEITIYSPINQEELGTVPAMTQTEADEAMQAARAALPAWRALSAVERAAYLHKTAAILERDKEEIGTILAKEVAKGIKAAIGEVVRTADLIRYAAEEGLRITGQAMEGGGFEAASKNKLAVVRREPVGIVLAIAPFNYPVNLSASKIAPALIAGNVVMFKPPTQGSISGLLLAKAFEEAGIPAGVFNTITGRGSEIGDYIIEHKEVNFINFTGSTPIGERIGRLAGMRPIMLELGGKDAALVLEDADLEHAAKQIVAGAFSYSGQRCTAIKRVIVLESVADKLATLLQEEVSKLTVGDPFDNADITPVIDNASADFIWGLIEDAQEKEAQALTPIKREGNLLWPVLFDQVTKDMKVAWEEPFGPVLPIIRVASVEEAIAFANESEFGLQSSVFTNDFKKAFEIAEKLEVGTVHINNKTQRGPDNFPFLGVKGSGAGVQGIKYSIEAMTNVKSIVFDVK

⊟Experimental data[edit | edit source]

  • protein localization:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulators: Rex* (repression) regulon, CodY (activation (repression)) regulon
    Rex*(TF)important in Energy metabolism; RegPrecise 
    CodY(TF)important in Amino acid metabolism; RegPrecise 

⊟Expression data[edit | edit source]

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

⊟Relevant publications[edit | edit source]

Simone Bergmann, Manfred Rohde, Sven Hammerschmidt
Glyceraldehyde-3-phosphate dehydrogenase of Streptococcus pneumoniae is a surface-displayed plasminogen-binding protein.
Infect Immun: 2004, 72(4);2416-9
[PubMed:15039372] [WorldCat.org] [DOI] (P p)Rémi Terrasse, Ana Amoroso, Thierry Vernet, Anne Marie Di Guilmi
Streptococcus pneumoniae GAPDH Is Released by Cell Lysis and Interacts with Peptidoglycan.
PLoS One: 2015, 10(4);e0125377
[PubMed:25927608] [WorldCat.org] [DOI] (I e)Xiaoyu Sun, Jichao Wang, Jie Zhou, Hong Wang, Xiaofang Wang, Jingwen Wu, Yujuan He, Yibing Yin, Xuemei Zhang, Wenchun Xu
Subcutaneous immunization with Streptococcus pneumoniae GAPDH confers effective protection in mice via TLR2 and TLR4.
Mol Immunol: 2017, 83;1-12
[PubMed:28088526] [WorldCat.org] [DOI] (I p)

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