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serotype 1

NCBI: 31-JAN-2014

⊟Summary[edit | edit source]

  • organism: Streptococcus pneumoniae JJA
  • locus tag: SPJ_1315 [new locus tag: SPJ_RS06530 ]
  • pan locus tag?: PNEUPAN002742000
  • symbol: queA
  • pan gene symbol?: queA
  • synonym:
  • product: S-adenosylmethionine:tRNA ribosyltransferase-isomerase

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SPJ_1315 [new locus tag: SPJ_RS06530 ]
  • symbol: queA
  • product: S-adenosylmethionine:tRNA ribosyltransferase-isomerase
  • replicon: chromosome
  • strand: +
  • coordinates: 1258900..1259928
  • length: 1029
  • essential: unknown

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    ATGAATACAGCTGATTTTGATTTCCACTTGCCAGAGGAATTGATTGCCCAAACGCCCCTT
    GAAAAACGTGATGCCTCCAAACTCCTCATCGTCAACCGTGAGACAGGAGAAATGCAAGAT
    AAACACTTCTACTCTATTATTGATATGCTGGAACCTGGTGATGCCCTTGTCATGAACGAC
    ACCCGAGTTCTCCCTGCCCGCCTCTATGGTCAAAAAGTGGAGACAGGAGGTCATGTGGAA
    CTTCTCCTCCTTAAGAACACTAGTGGAGACGAGTGGGAAGTTCTGGCTAAACCTGCCAAA
    CGCCTCAAGGTCGGTACTCGTATCAGCTTTGGTGATGGCCGCCTCAGCGCTGTCGTTACA
    GAAGAATTGACCCACGGGGGACGCATTGTCCGCTTTGAATACCAAGGAATTTTCCTAGAA
    GTCTTGGAAAGTCTGGGAGAAATGCCTCTGCCACCTTATATCCACGAAAAATTAGATGAC
    CGTGAACGTTATCAAACCGTCTACGCCAAGGAAAGCGGCTCTGCTGCAGCACCGACTGCT
    GGTCTTCACTTCACCAAAGAACTGCTGGCAGAAATCCAAGCTAAGGGTGTTCATCTAGTC
    TATCTGACTCTCCATGTCGGACTCGGAACCTTTAGACCTGTTTCTGTGGATAATCTGGAC
    GAACACGAAATGCACTCAGAGTTCTATCAACTTTCTGAGGAAGCTGCTGCCACCCTTCGC
    TCTGTCAAAGAAAATGGTGGTCGCGTCATCGCTGTCGGAACCACTTCTATCCGCACCTTG
    GAAACTATTGGTTCCAAGTTTGATGGGCAAATCCAAGCAGATTCTGGTTGGACCAATATC
    TTTATCAAACCTGGGTATGAGTGGAAGGTCGTGGATGCCTTCTCAACCAACTTCCATCTG
    CCAAAATCAACTCTGGTCATGTTGGTTTCTGCCTTTGCAGGCCGTGAATTAGTCTTAGAT
    GCCTACCACCATGCCATCCAAGAACACTACCGCTTCTTCAGTTTTGGTGACGCCATGTTT
    ATTTATTGA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1029

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SPJ_1315 [new locus tag: SPJ_RS06530 ]
  • symbol: QueA
  • description: S-adenosylmethionine:tRNA ribosyltransferase-isomerase
  • length: 342
  • theoretical pI: 5.4023
  • theoretical MW: 38390.4
  • GRAVY: -0.183333

⊟Function[edit | edit source]

  • reaction:
    EC 5.-.-.-?  ExPASy
  • TIGRFAM:
    Genetic information processing Protein synthesis tRNA and rRNA base modification S-adenosylmethionine:tRNA ribosyltransferase-isomerase (TIGR00113; EC 2.4.99.17; HMM-score: 461.9)
  • TheSEED  :
    • tRNA preQ1(34) S-adenosylmethionine ribosyltransferase-isomerase (EC 2.4.99.17)
    RNA Metabolism RNA processing and modification Queuosine-Archaeosine Biosynthesis  S-adenosylmethionine:tRNA ribosyltransferase-isomerase (EC 5.-.-.-)
  • PFAM:
    no clan defined Queuosine_synth; Queuosine biosynthesis protein (PF02547; HMM-score: 467.8)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.003122
    • TAT(Tat/SPI): 0.000258
    • LIPO(Sec/SPII): 0.000367
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MNTADFDFHLPEELIAQTPLEKRDASKLLIVNRETGEMQDKHFYSIIDMLEPGDALVMNDTRVLPARLYGQKVETGGHVELLLLKNTSGDEWEVLAKPAKRLKVGTRISFGDGRLSAVVTEELTHGGRIVRFEYQGIFLEVLESLGEMPLPPYIHEKLDDRERYQTVYAKESGSAAAPTAGLHFTKELLAEIQAKGVHLVYLTLHVGLGTFRPVSVDNLDEHEMHSEFYQLSEEAAATLRSVKENGGRVIAVGTTSIRTLETIGSKFDGQIQADSGWTNIFIKPGYEWKVVDAFSTNFHLPKSTLVMLVSAFAGRELVLDAYHHAIQEHYRFFSFGDAMFIY

⊟Experimental data[edit | edit source]

  • protein localization:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator: CcpA regulon
    CcpA(TF)important in Global catabolite repression; RegPrecise 

⊟Expression data[edit | edit source]

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

⊟Relevant publications[edit | edit source]

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