PangenomeTIGR4

serotype 4

D39

serotype 2

D39V

serotype 2

Hungary19A-6

serotype 19A

EF3030

serotype 19F

670-6B

serotype 6B

6A-10

serotype 6A

70585

serotype 5

A026

serotype 19F

A66

serotype 3

AP200

serotype 11A

ASP0581

serotype 12F

ATCC 49619

serotype 19F

ATCC 700669

serotype 23F

BM6001

serotype 19F

BVJ1JL

serotype 1

CGSP14

serotype 14

G54

serotype 19F

HU-OH

serotype 3

Hu15

serotype 19A

Hu17

serotype 19A

INV104

serotype 1

INV200

serotype 14

JJA

serotype 14

MDRSPN001

serotype 19F

NCTC7465

serotype 1

NCTC7466

serotype 2

NU83127

serotype 4

OXC141

serotype 3

P1031

serotype 1

R6

serotype 2

SP49

serotype 19A

SPN032672

serotype 1

SPN034156

serotype 3

SPN034183

serotype 3

SPN994038

serotype 3

SPN994039

serotype 3

SPNA45

serotype 3

ST556

serotype 19F

TCH8431/19A

serotype 19A

Taiwan19F-14

serotype 19F

Xen35

serotype 4

gamPNI0373

serotype 1

NCBI: 05-APR-2025

⊟Summary

• organism: Streptococcus pneumoniae JJA
• locus tag: SPJ_RS03740 [old locus tag: SPJ_0751 ]
• pan locus tag^?: PNEUPAN001756000
• symbol: SPJ_RS03740
• pan gene symbol^?: gph_2
• synonym:
• product: HAD family hydrolase

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: SPJ_RS03740 [old locus tag: SPJ_0751 ]
• symbol: SPJ_RS03740
• product: HAD family hydrolase
• replicon: chromosome
• strand: +
• coordinates: 720717..721280
• length: 564
• essential: unknown

⊟Accession numbers

• Location: NC_012466 (720717..721280) NCBI
• BioCyc: SPJ_RS03740 BioCyc
• MicrobesOnline: see SPJ_0751
• PneumoBrowse for strain D39V: SPV_0708 PneumoBrowse

⊟Phenotype

Share your knowledge and add information here. [edit]

⊟DNA sequence

• 1
  61
  121
  181
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  301
  361
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  481
  541
  
  ATGAAATATCACGATTACATCTGGGATTTAGGTGGAACTTTACTGGATAATTATGAAACT
  TCAACAGCTGCATTTGTTGAAACATTGGCACTGTATGGTATCACACAAGACCATGACAGT
  GTCTATCAAGCTTTAAAGGTTTCTACTCCTTTTGCGATTGAGACATTCGCTCCCAATTTA
  GAGAATTTTTTAGAAAAGTACAAGGAAAATGAAGCCAGAGAGCTTGAACACCCGATTTTA
  TTTGAAGGAGTTTCTGACCTATTGGAAGACATTTTAAATCAAGGTGGCCGTCATTTTTTG
  GTCTCTCATCGAAATGATCAGGTTTTGGAAATTTTAGAAAAAACCTCTATAGCAGCTTAT
  TTTACAGAAGTGGTGACTTCTAGCTCAGGCTTTAAGAGAAAGCCAAATCCCGAATCCATG
  CTTTATTTAAGAGAAAAGTATCAGATTAGCTCTGGTCTTGTCATTGGTGATCGGCCGATT
  GATATCGAAGCAGGTCAAGCTGCAGGACTTGATACCCACTTGTTTACCAGTATCGTGAAT
  TTAAGACAAGTATTAGACATATAA
  60
  120
  180
  240
  300
  360
  420
  480
  540
  564
  

⊟Protein

⊟General

• locus tag: SPJ_RS03740 [old locus tag: SPJ_0751 ]
• symbol: SPJ_RS03740
• description: HAD family hydrolase
• length: 187
• theoretical pI: 4.44688
• theoretical MW: 21159.6
• GRAVY: -0.206417

⊟Function

• TIGRFAM:
  Unknown function Enzymes of unknown specificity HAD hydrolase, family IA, variant 1 (TIGR01549; HMM-score: 46.7)
  Metabolism Energy metabolism Sugars phosphoglycolate phosphatase, bacterial (TIGR01449; EC 3.1.3.18; HMM-score: 43.2)
  and 11 more
  beta-phosphoglucomutase family hydrolase (TIGR02009; HMM-score: 34.3)
  HAD hydrolase, REG-2-like, family IA (TIGR02252; HMM-score: 29.4)
  AHBA synthesis associated protein (TIGR01454; HMM-score: 28.5)
  noncanonical pyrimidine nucleotidase, YjjG family (TIGR02254; EC 3.1.3.5; HMM-score: 28.4)
  HAD hydrolase, TIGR02253 family (TIGR02253; HMM-score: 24.9)
  phosphonatase-like hydrolase (TIGR03351; HMM-score: 23.6)
  Unknown function Enzymes of unknown specificity HAD hydrolase, family IIIA (TIGR01662; HMM-score: 23.5)
  Metabolism Energy metabolism Biosynthesis and degradation of polysaccharides beta-phosphoglucomutase (TIGR01990; EC 5.4.2.6; HMM-score: 22.6)
  histidinol-phosphate phosphatase domain (TIGR01656; HMM-score: 20.8)
  Unknown function Enzymes of unknown specificity HAD hydrolase, family IA, variant 3 (TIGR01509; HMM-score: 20.2)
  Unknown function Enzymes of unknown specificity HAD hydrolase, family IIA (TIGR01460; HMM-score: 15)
• TheSEED: see SPJ_0751
• PFAM:
  HAD (CL0137) HAD_2; haloacid dehalogenase-like hydrolase (PF13419; HMM-score: 82.2)
  and 5 more
  Hydrolase; haloacid dehalogenase-like hydrolase (PF00702; HMM-score: 28.7)
  Hydrolase_like; HAD-hyrolase-like (PF13242; HMM-score: 26.9)
  HAD; haloacid dehalogenase-like hydrolase (PF12710; HMM-score: 25.6)
  YjgF-like (CL0534) YjgF_endoribonc; YjgF/chorismate_mutase-like, putative endoribonuclease (PF14588; HMM-score: 14.2)
  no clan defined Nore1-SARAH; Novel Ras effector 1 C-terminal SARAH (Sav/Rassf/Hpo) domain (PF16517; HMM-score: 12.7)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: Cytoplasmic
  • Cytoplasmic Score: 7.5
  • Cytoplasmic Membrane Score: 1.15
  • Cellwall Score: 0.62
  • Extracellular Score: 0.73
  • Internal Helices: 0
• DeepLocPro: Cytoplasmic
  • Cytoplasmic Score: 0.9898
  • Cytoplasmic Membrane Score: 0.0059
  • Cell wall & surface Score: 0.0005
  • Extracellular Score: 0.0039
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.006974
  • TAT(Tat/SPI): 0.000341
  • LIPO(Sec/SPII): 0.000603
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI:
• RefSeq: WP_001812999 NCBI
• UniProt: see SPJ_0751

⊟Protein sequence

• MKYHDYIWDLGGTLLDNYETSTAAFVETLALYGITQDHDSVYQALKVSTPFAIETFAPNLENFLEKYKENEARELEHPILFEGVSDLLEDILNQGGRHFLVSHRNDQVLEILEKTSIAAYFTEVVTSSSGFKRKPNPESMLYLREKYQISSGLVIGDRPIDIEAGQAAGLDTHLFTSIVNLRQVLDI

⊟Experimental data

• protein localization:
• interaction partners:

⊟Expression & Regulation

⊟Operon

• MicrobesOnline: SPJ_RS03740 > gyrB > ezrA

⊟Regulation

• regulator:

⊟Expression data

• PneumoExpress for strain D39V: SPV_0708 PneumoExpress

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You can add further information about the gene and protein here. [edit]

⊟Literature

⊟References

⊟Relevant publications