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PangenomeTIGR4
serotype 4
D39
serotype 2
D39V
serotype 2
Hungary19A-6
serotype 19A
EF3030
serotype 19F
670-6B
serotype 6B
6A-10
serotype 6A
70585
serotype 5
A026
serotype 19F
A66
serotype 3
AP200
serotype 11A
ASP0581
serotype 12F
ATCC 49619
serotype 19F
ATCC 700669
serotype 23F
BM6001
serotype 19F
BVJ1JL
serotype 1
CGSP14
serotype 14
G54
serotype 19F
HU-OH
serotype 3
Hu15
serotype 19A
Hu17
serotype 19A
INV104
serotype 1
INV200
serotype 14
JJA
serotype 14
MDRSPN001
serotype 19F
NCTC7465
serotype 1
NCTC7466
serotype 2
NU83127
serotype 4
OXC141
serotype 3
P1031
serotype 1
R6
serotype 2
SP49
serotype 19A
SPN032672
serotype 1
SPN034156
serotype 3
SPN034183
serotype 3
SPN994038
serotype 3
SPN994039
serotype 3
SPNA45
serotype 3
ST556
serotype 19F
TCH8431/19A
serotype 19A
Taiwan19F-14
serotype 19F
Xen35
serotype 4
gamPNI0373
serotype 1

NCBI: 11-FEB-2025

⊟Summary[edit | edit source]

  • organism: Streptococcus pneumoniae SPN032672
  • locus tag: SPN032672_RS00470
  • pan locus tag?: PNEUPAN002256000
  • symbol: ligA
  • pan gene symbol?: ligA
  • synonym:
  • product: NAD-dependent DNA ligase LigA

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SPN032672_RS00470
  • symbol: ligA
  • product: NAD-dependent DNA ligase LigA
  • replicon: chromosome
  • strand: +
  • coordinates: 84105..86063
  • length: 1959
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

  • Location: NC_021003 (84105..86063) NCBI
  • BioCyc:
  • MicrobesOnline:
  • PneumoBrowse for strain D39V: SPV_1001 PneumoBrowse

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    ATGAATAAAAGAATGAATGAGTTAGTCGCTTTGCTCAATCGCTATGCGACTGAGTACTAT
    ACCAGCGATAATCCCTCGGTTTCAGACAGTGAGTATGACCGCCTTTACCGTGAGTTGGTC
    GAGTTAGAAACTGCTTATCCAGAGCAAGTGCTAGCAGACAGTCCGACTCATCGTGTTGGT
    GGCAAGGTTTTAGATGGTTTTGAAAAATACAGTCATCAGTATCCTCTTTATAGTTTGCAG
    GATGCTTTTTCACGTGAGGAGCTAGATGCTTTTGATGCGCGTGTTCGTAAGGAAGTGGCT
    CATCCGACCTATATTTGTGAGCTGAAAATCGATGGCTTATCTATCTCGCTGACTTATGAA
    AAGGGGATTTTGGTTGCTGGGGTAACACGTGGAGATGGTTCAATTGGTGAAAATATCACA
    GAAAACCTCAAGCGTGTTAAGGACATCCCTTTGACTTTGCCAGAAGAACTAGATATCACA
    GTTCGTGGGGAATGTTACATGCCACGCGCTTCCTTTGACCAAGTTAACCAAGTGCGCCAA
    GAAAATGGAGAGCCTGAATTTGCTAATCCTCGTAATGCGGCAGCAGGAACTCTGCGTCAG
    TTGGATACAGCAGTAGTTGCCAAGCGTAATCTTGCAACGTTTCTCTATCAAGAAGCCAGC
    CCTTCAACTCGTGATAGCCAAGAAAAGGGTTTGAAGTACCTAGAACAACTAGGTTTTGTG
    GTCAATCCTAAGCGAATCTTGGCTGAAAACATAGATGAAATCTGGAATTTTATCCAAGAA
    GTAGGACAGGAACGGGAAAATCTGCCTTACGATATTGATGGAGTGGTAATCAAGGTCAAC
    GACCTAGCAAGTCAAGAAGAACTTGGTTTTACCGTTAAGGCTCCAAAGTGGGCAGTAGCC
    TACAAGTTCCCTGCTGAAGAAAAAGAAGCTCAACTCTTATCAGTTGACTGGACAGTTGGC
    CGTACCGGTGTTGTAACTCCAACTGCTAATCTAACACCAGTACAACTTGCCGGTACGACT
    GTTAGCCGTGCGACCCTGCACAATGTAGATTATATTGCTGAAAAAGATATCCGAAAAGAC
    GATACGGTCATTGTATATAAGGCTGGTGACATCATCCCTGCCGTTTTACGTGTGGTAGAG
    TCCAAACGGGTTTCTGAAGAAAAACTAGATATCCCTACAAACTGTCCAAGTTGTAACTCT
    GACTTGTTGCACTTTGAAGATGAAGTGGCCCTACGTTGTATCAATCCGCGTTGCCCTGCT
    CAAATCATGGAAGGCTTGATTCACTTTGCTTCTCGTGATGCTATGAATATTACAGGCCTT
    GGTCCATCTATTGTTGAGAAGCTTTTTGCTGCTAATTTAGTCAAGGATGTGGCGGATATT
    TATCGTTTGCAAGAAGAGGATTTCCTCCTTTTAGAGGGGGTTAAGGAAAAGTCCGCTGCT
    AAACTGTATCAGGCTATCCAAGCATCAAAGGAAAATTCTGCCGAGAAGCTCTTATTTGGT
    TTGGGAATTCGTCATGTCGGAAGCAAGGCTAGTCAGCTTTTACTTCAATATTTCCATTCA
    ATTGAAAATCTGTCTCAGGCAGATTCAGAGGAAGTGGCTAGTATTGAAAGTCTAGGTGGC
    GTGATTGCCAAAAGTCTTCAGACTTATTTTGCGACAGAAGGCTCTGAAATTCTGCTCAGA
    GAATTGAAAGAAACTGGGGTCAATCTGGACTATAAAGGACAGACGGTAGTAGCGGATGCG
    GCCTTGTCAGGTTTGACCGTGGTATTGACAGGAAAATTGGAACGACTCAAGCGCTCAGAA
    GCTAAAAGTAAACTCGAAAGTCTGGGTGCCAAAGTGACAGGTAGTGTTTCTAAAAAGACC
    GACCTCGTCGTGGTAGGTGCAGACGCTGGAAGTAAACTGCAAAAAGCACAAGAACTTGGT
    ATCCAGGTCAGAGATGAGGCATGGCTAGAAAGTTTGTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1320
    1380
    1440
    1500
    1560
    1620
    1680
    1740
    1800
    1860
    1920
    1959

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SPN032672_RS00470
  • symbol: LigA
  • description: NAD-dependent DNA ligase LigA
  • length: 652
  • theoretical pI: 4.71784
  • theoretical MW: 72227.3
  • GRAVY: -0.274693

⊟Function[edit | edit source]

  • reaction:
    EC 6.5.1.2?  ExPASy
    DNA ligase (NAD+) NAD+ + (deoxyribonucleotide)(n)-3'-hydroxyl + 5'-phospho-(deoxyribonucleotide)(m) = (deoxyribonucleotide)(n+m) + AMP + beta-nicotinamide D-nucleotide
  • TIGRFAM:
    Genetic information processing DNA metabolism DNA replication, recombination, and repair DNA ligase, NAD-dependent (TIGR00575; EC 6.5.1.2; HMM-score: 803.7)
  • TheSEED: data available for D39, Hungary19A-6, TIGR4
  • PFAM:
    DNA_ligase (CL0078) DNA_ligase_aden; NAD-dependent DNA ligase adenylation domain (PF01653; HMM-score: 387)
    and 7 more
    OB (CL0021) DNA_ligase_OB; NAD-dependent DNA ligase OB-fold domain (PF03120; HMM-score: 115.5)
    HHH (CL0198) HHH_2; Helix-hairpin-helix motif (PF12826; HMM-score: 69.1)
    BRCT-like (CL0459) BRCT; BRCA1 C Terminus (BRCT) domain (PF00533; HMM-score: 55.3)
    HHH (CL0198) HHH_5; Helix-hairpin-helix domain (PF14520; HMM-score: 36.6)
    no clan defined DNA_ligase_ZBD; NAD-dependent DNA ligase C4 zinc finger domain (PF03119; HMM-score: 34.8)
    BRCT-like (CL0459) PTCB-BRCT; twin BRCT domain (PF12738; HMM-score: 25.6)
    5_3_exonuc_C (CL0464) 5_3_exonuc; 5'-3' exonuclease, C-terminal SAM fold (PF01367; HMM-score: 13.6)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • DeepLocPro: Cytoplasmic
    • Cytoplasmic Score: 0.9984
    • Cytoplasmic Membrane Score: 0.0003
    • Cell wall & surface Score: 0.0002
    • Extracellular Score: 0.0011
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.012256
    • TAT(Tat/SPI): 0.000628
    • LIPO(Sec/SPII): 0.001876
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

  • GI:
  • RefSeq: WP_001042594 NCBI
  • UniProt:

⊟Protein sequence[edit | edit source]

  • MNKRMNELVALLNRYATEYYTSDNPSVSDSEYDRLYRELVELETAYPEQVLADSPTHRVGGKVLDGFEKYSHQYPLYSLQDAFSREELDAFDARVRKEVAHPTYICELKIDGLSISLTYEKGILVAGVTRGDGSIGENITENLKRVKDIPLTLPEELDITVRGECYMPRASFDQVNQVRQENGEPEFANPRNAAAGTLRQLDTAVVAKRNLATFLYQEASPSTRDSQEKGLKYLEQLGFVVNPKRILAENIDEIWNFIQEVGQERENLPYDIDGVVIKVNDLASQEELGFTVKAPKWAVAYKFPAEEKEAQLLSVDWTVGRTGVVTPTANLTPVQLAGTTVSRATLHNVDYIAEKDIRKDDTVIVYKAGDIIPAVLRVVESKRVSEEKLDIPTNCPSCNSDLLHFEDEVALRCINPRCPAQIMEGLIHFASRDAMNITGLGPSIVEKLFAANLVKDVADIYRLQEEDFLLLEGVKEKSAAKLYQAIQASKENSAEKLLFGLGIRHVGSKASQLLLQYFHSIENLSQADSEEVASIESLGGVIAKSLQTYFATEGSEILLRELKETGVNLDYKGQTVVADAALSGLTVVLTGKLERLKRSEAKSKLESLGAKVTGSVSKKTDLVVVGADAGSKLQKAQELGIQVRDEAWLESL

⊟Experimental data[edit | edit source]

  • protein localization:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator:

⊟Expression data[edit | edit source]

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You can add further information about the gene and protein here. [edit]

⊟Literature[edit | edit source]

⊟References[edit | edit source]

⊟Relevant publications[edit | edit source]

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