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PangenomeTIGR4
serotype 4
D39
serotype 2
D39V
serotype 2
Hungary19A-6
serotype 19A
EF3030
serotype 19F
670-6B
serotype 6B
6A-10
serotype 6A
70585
serotype 5
A026
serotype 19F
A66
serotype 3
AP200
serotype 11A
ASP0581
serotype 12F
ATCC 49619
serotype 19F
ATCC 700669
serotype 23F
BVJ1JL
serotype 1
CGSP14
serotype 14
G54
serotype 19F
HU-OH
serotype 3
Hu15
serotype 19A
Hu17
serotype 19A
INV104
serotype 1
INV200
serotype 14
JJA
serotype 14
MDRSPN001
serotype 19F
NCTC7466
serotype 2
NU83127
serotype 4
OXC141
serotype 3
P1031
serotype 1
R6
serotype 2
SP49
serotype 19A
SPN032672
serotype 1
SPN034156
serotype 3
SPN034183
serotype 3
SPN994038
serotype 3
SPN994039
serotype 3
SPNA45
serotype 3
ST556
serotype 19F
TCH8431/19A
serotype 19A
Taiwan19F-14
serotype 19F
Xen35
serotype 4
gamPNI0373
serotype 1

NCBI: 06-FEB-2015

⊟Summary[edit | edit source]

  • organism: Streptococcus pneumoniae ATCC 700669
  • locus tag: SPN23F10810 [new locus tag: SPN23F_RS05490 ]
  • pan locus tag?: PNEUPAN002445000
  • symbol: SPN23F10810
  • pan gene symbol?: nrdE
  • synonym:
  • product: putative ribonucleoside-diphosphate reductase alpha chain

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SPN23F10810 [new locus tag: SPN23F_RS05490 ]
  • symbol: SPN23F10810
  • product: putative ribonucleoside-diphosphate reductase alpha chain
  • replicon: chromosome
  • strand: +
  • coordinates: 1059087..1061246
  • length: 2160
  • essential: unknown

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    ATGGGATTAAAACATCTTGAAGACGTGACTTACTTCCGTCTTAATAACGAAATTAACCGT
    CCTGTTAATGGACAAATCATGCTTCATAAAGATAAGGAAGCCTTGGATGCTTTCTTTAAA
    GAAAATGTAGTTCCAAACACTATGGTTTTTGATTCAATCAAAGACAAAATCAATTACCTC
    ATTGAACACAACTACATCGAAACAGCCTTTATCAAGAAATACCGTCCAGAGTTCTTGGAA
    GAATTGGCTCAATTTATCAAAGACCAAAACTTCCAATTCAAGTCATTCATGGCAGCTTAT
    AAATTTTACAATCAATATGCCTTAAAGACTAATGACGGTGAATACTATCTTGAAAATATG
    GAAGACCGTGTCTTCTTCAACGCCCTTTATTTCGCTGATGGGAATGAAGCTGTTGCAATC
    GATATTGCCAATGAAATCATCCACCAACGCTACCAACCTGCTACTCCTTCCTTCTTGAAT
    GCTGGACGTGCTCGTCGTGGGGAGTTGGTATCTTGTTTCCTAATCCAAGTGACGGATGAT
    ATGAACTCTATCGGGCGTTCTATCAACTCAGCTCTTCAACTTTCACGTATTGGTGGTGGT
    GTGGGAATTACCCTCAGCAACCTTCGTGAAGCTGGTGCACCTATCAAAGGCTATGAAGGA
    GCTGCTTCAGGTGTCGTTCCTGTTATGAAGCTTTTTGAAGACAGCTTCTCTTACTCCAAC
    CAATTGGGGCAACGTCAAGGTGCCGGCGTTGTCTACCTCAACGTCTTTCACCCAGATATC
    ATCGCTTTCCTTTCAACTAAGAAAGAAAACGCTGATGAAAAAGTACGTGTCAAGACTCTA
    TCACTTGGTGTTGTAGTACCTGATAAATTCTACGAATTGGCTCGTAAAAATGAAGAAATG
    TACCTCTTCAGCCCTTACTCTGTAGAAAAAGAATACGGTGTGCCATTCAATTACATCGAC
    ATTACTGAAAAATACGATGAATTGGTCGCAAATCCAAATATTCGCAAGACAAAAATCAAG
    GCGCGGGATTTGGAAACTGAAATCTCTAAATTGCAACAAGAGTCTGGCTATCCTTATGTA
    GTCAACATCGATACGGCTAACCGTGCAAATCCTGTTGATGGAAAGATTATCATGAGTAAC
    TTGTGTTCTGAGATTCTTCAAGTTCAAGAACCAAGCTTGATCAACGATGCTCAAGAATTC
    CTTCAAATGGGAACAGATGTTTCATGTAACCTGGGTTCAACCAACGTGGTTAACATGATG
    ACTTCACCTGATTTTGGTCGTTCTATCCGTGCTATGGTTCGTGCCCTTACTTTCGTTACA
    GATAGTTCACATATCGTAGCTGTACCAACGATTGACCATGGAAATAGCCAAGCCCACACC
    TTTGGTCTCGGTGCCATGGGACTGCACAGCTACCTTGCCCAACAACTCATTGAATATGGT
    TCACCTGAGTCTGTTGAATTTACAAGCATCTACTTTATGCTTATGAACTACTGGACCTTG
    GTAGAGTCAAACAATATTGCACGTGAACGTGGTATTACCTTCCACAACTTTGAAAAATCA
    GACTATGCTAACGGAAGTTACTTTGACAAGTATGTAACTGGTGAATTTGTTCCAACATCA
    GACCGTGTTAAAGAACTCTTCAAAAATGTTTTTATCCCTGGTGTTGCTGATTGGGCTGAA
    CTTCGCGACAAGGTTCAAGAAGATGGTCTTTACCACCAAAATCGCCTTGCTGTAGCGCCA
    AATGGTTCTATCAGCTATATCAACGACGTTTCTGCTTCTATCCACCCGATTACGCAACGT
    ATCGAAGAACGCCAAGAGAAGAAAATTGGTAAAATCTACTACCCTGCTGCTGGCTTGTCT
    ACAGAAACCATTCCTTACTACACTTCTGCCTACGATATGGATATGCGTAAAGTCATCGAT
    GTTTACGCTGCTGCGACTGAACACGTGGATCAAGGACTTTCACTCACCCTCTTCATGCGC
    AGTGACATTCCAAAAGGCCTTTACGAATGGAAGAGGGAAAATAAACAAACGACACGTGAT
    TTGTCCATCCTTCGTAACTATGCCTTTAACAAGGGAATCAAGTCTATCTACTACGTCCGT
    ACCTTTACAGATGACGGTGGAGAAGTCGGCGCCAACCAATGTGAAAGCTGTGTGATTTAG
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1320
    1380
    1440
    1500
    1560
    1620
    1680
    1740
    1800
    1860
    1920
    1980
    2040
    2100
    2160

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SPN23F10810 [new locus tag: SPN23F_RS05490 ]
  • symbol: SPN23F10810
  • description: putative ribonucleoside-diphosphate reductase alpha chain
  • length: 719
  • theoretical pI: 5.14378
  • theoretical MW: 81591.7
  • GRAVY: -0.315994

⊟Function[edit | edit source]

  • reaction:
    EC 1.17.4.1?  ExPASy
    Ribonucleoside-diphosphate reductase 2'-deoxyribonucleoside diphosphate + thioredoxin disulfide + H2O = ribonucleoside diphosphate + thioredoxin
  • TIGRFAM:
    ribonucleoside-diphosphate reductase, class 1b, alpha subunit (TIGR04170; EC 1.17.4.1; HMM-score: 1228.4)
    and 4 more
    Metabolism Purines, pyrimidines, nucleosides, and nucleotides 2'-Deoxyribonucleotide metabolism ribonucleoside-diphosphate reductase, alpha subunit (TIGR02506; EC 1.17.4.1; HMM-score: 492.7)
    Metabolism Purines, pyrimidines, nucleosides, and nucleotides 2'-Deoxyribonucleotide metabolism ribonucleoside-diphosphate reductase, adenosylcobalamin-dependent (TIGR02504; EC 1.17.4.1; HMM-score: 233.9)
    Metabolism Purines, pyrimidines, nucleosides, and nucleotides 2'-Deoxyribonucleotide metabolism ribonucleoside-diphosphate reductase, alpha chain (TIGR02510; EC 1.17.4.1; HMM-score: 92.3)
    Metabolism Purines, pyrimidines, nucleosides, and nucleotides 2'-Deoxyribonucleotide metabolism ribonucleoside-triphosphate reductase, adenosylcobalamin-dependent (TIGR02505; EC 1.17.4.2; HMM-score: 21.8)
  • TheSEED  :
    • Ribonucleotide reductase of class Ib (aerobic), alpha subunit (EC 1.17.4.1)
    Nucleosides and Nucleotides Nucleosides and Nucleotides - no subcategory Ribonucleotide reduction  Ribonucleotide reductase of class Ib (aerobic), alpha subunit (EC 1.17.4.1)
    and 1 more
    Stress Response Oxidative stress Glutathione: Redox cycle  Ribonucleotide reductase of class Ib (aerobic), alpha subunit (EC 1.17.4.1)
  • PFAM:
    PFL-like (CL0339) Ribonuc_red_lgC; Ribonucleotide reductase, barrel domain (PF02867; HMM-score: 468.1)
    and 2 more
    no clan defined RNR_N; Ribonucleotide reductase N-terminal (PF08343; HMM-score: 97.2)
    Ribonuc_red_lgN; Ribonucleotide reductase, all-alpha domain (PF00317; HMM-score: 37.8)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.002216
    • TAT(Tat/SPI): 0.000122
    • LIPO(Sec/SPII): 0.00044
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

  • GI:
  • RefSeq: CAR68895 NCBI
  • UniProt:

⊟Protein sequence[edit | edit source]

  • MGLKHLEDVTYFRLNNEINRPVNGQIMLHKDKEALDAFFKENVVPNTMVFDSIKDKINYLIEHNYIETAFIKKYRPEFLEELAQFIKDQNFQFKSFMAAYKFYNQYALKTNDGEYYLENMEDRVFFNALYFADGNEAVAIDIANEIIHQRYQPATPSFLNAGRARRGELVSCFLIQVTDDMNSIGRSINSALQLSRIGGGVGITLSNLREAGAPIKGYEGAASGVVPVMKLFEDSFSYSNQLGQRQGAGVVYLNVFHPDIIAFLSTKKENADEKVRVKTLSLGVVVPDKFYELARKNEEMYLFSPYSVEKEYGVPFNYIDITEKYDELVANPNIRKTKIKARDLETEISKLQQESGYPYVVNIDTANRANPVDGKIIMSNLCSEILQVQEPSLINDAQEFLQMGTDVSCNLGSTNVVNMMTSPDFGRSIRAMVRALTFVTDSSHIVAVPTIDHGNSQAHTFGLGAMGLHSYLAQQLIEYGSPESVEFTSIYFMLMNYWTLVESNNIARERGITFHNFEKSDYANGSYFDKYVTGEFVPTSDRVKELFKNVFIPGVADWAELRDKVQEDGLYHQNRLAVAPNGSISYINDVSASIHPITQRIEERQEKKIGKIYYPAAGLSTETIPYYTSAYDMDMRKVIDVYAAATEHVDQGLSLTLFMRSDIPKGLYEWKRENKQTTRDLSILRNYAFNKGIKSIYYVRTFTDDGGEVGANQCESCVI

⊟Experimental data[edit | edit source]

  • protein localization:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator: NrdR* (repression) regulon
    NrdR*(TF)important in Deoxyribonucleotide biosynthesis; RegPrecise    transcription unit transferred from TIGR4 data RegPrecise 

⊟Expression data[edit | edit source]

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

⊟Relevant publications[edit | edit source]

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