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PangenomeTIGR4
serotype 4
D39serotype 2
D39Vserotype 2
Hungary19A-6serotype 19A
EF3030serotype 19F
670-6Bserotype 6B
6A-10serotype 6A
70585serotype 5
A026serotype 19F
A66serotype 3
AP200serotype 11A
ASP0581serotype 12F
ATCC 49619serotype 19F
ATCC 700669serotype 23F
BVJ1JLserotype 1
CGSP14serotype 14
G54serotype 19F
HU-OHserotype 3
Hu15serotype 19A
Hu17serotype 19A
INV104serotype 1
INV200serotype 14
JJAserotype 14
MDRSPN001serotype 19F
NCTC7466serotype 2
NU83127serotype 4
OXC141serotype 3
P1031serotype 1
R6serotype 2
SP49serotype 19A
SPN032672serotype 1
SPN034156serotype 3
SPN034183serotype 3
SPN994038serotype 3
SPN994039serotype 3
SPNA45serotype 3
ST556serotype 19F
TCH8431/19Aserotype 19A
Taiwan19F-14serotype 19F
Xen35serotype 4
gamPNI0373serotype 1
NCBI: 13-DEC-2020
⊟Summary[edit | edit source]
- organism: Streptococcus pneumoniae ATCC 700669
- locus tag: SPN23F_RS04075 [old locus tags: SPN23F07980 SPN23F_07980 ]
- pan locus tag?: PNEUPAN001859000
- symbol: pfkB
- pan gene symbol?: fruB
- synonym:
- product: 1-phosphofructokinase
⊟Genome View[edit | edit source]
⊟Gene[edit | edit source]
⊟General[edit | edit source]
- type: CDS
- locus tag: SPN23F_RS04075 [old locus tags: SPN23F07980 SPN23F_07980 ]
- symbol: pfkB
- product: 1-phosphofructokinase
- replicon: chromosome
- strand: +
- coordinates: 777369..778280
- length: 912
- essential: unknown
⊟Accession numbers[edit | edit source]
- Location: NC_011900 (777369..778280) NCBI
- BioCyc: SPN23F_RS04075 BioCyc
- MicrobesOnline: see SPN23F07980
- PneumoBrowse for strain D39V: SPV_0772 PneumoBrowse
⊟Phenotype[edit | edit source]
Share your knowledge and add information here. [edit]
⊟DNA sequence[edit | edit source]
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901ATGATTTATACAGTCACACTCAATCCATCCATTGACTATATCGTTCGTTTGGACCAAGTC
AAAGTTGGTAGTGTAAATCGTATGGACAGTGATGATAAGTTTGCTGGTGGGAAAGGAATC
AATGTCAGCCGTGTCTTGAAACGTTTGAATATACCAAATACAGCGACGGGATTTATCGGT
GGCTTTACTGGTAAATTTATCACAGATACTTTAGCAGAGGAAGAAATCGAGACACGTTTT
GTCCAGGTGGCAGAAGATACTCGTATCAATGTTAAAATCAAAGCAGACCAAGAAACAGAA
ATCAACGGAACGGGTCCAACTGTTGAACCGGTTCAGCTAGAAGAATTGAAAGCTATTTTA
TCTAGTCTGACAGCAGAAGATACAGTTGTCTTTGCAGGTTCAAGTGCTAAAAATCTAGGC
AATGTTATCTATAAGGATTTGATTTCCTTGACGCGCCAGACTGGTGCGCAAGTGGTCTGT
GACTTTGAAGGACAGACCTTAATTGATAGTTTGGACTACCAGCCTCTTCTTGTAAAACCA
AACAATCATGAACTTGGAGCGATTTTTGGGGTTAAACTCGAAAGTTTAGATGAAATTGAG
AAATACGCTCGTGAGTTACTGGCTAAGGGTGCTCAAAATGTTATTATCTCTATGGCTGGT
GATGGCGCCCTTCTTGTCACATCTGAGGGAGCTTACTTCGCTAAACCAATCAAAGGAACA
GTCAAAAATTCAGTTGGAGCTGGTGATTCTATGGTTGCTGGATTCACAGGTGAATTTGTC
AAATCAAAAGACGCAGTAGAAGCCTTCAAATGGGGAGTGGCTTGCGGAACGGCAACTACC
TTCTCAGATGACTTGGCAACGGCGGAATTTATTAAAGAAACATATGGAAAAGTTGAGGTA
GAAAAAAGATGA60
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⊟Protein[edit | edit source]
⊟General[edit | edit source]
- locus tag: SPN23F_RS04075 [old locus tags: SPN23F07980 SPN23F_07980 ]
- symbol: PfkB
- description: 1-phosphofructokinase
- length: 303
- theoretical pI: 4.54145
- theoretical MW: 32650.8
- GRAVY: -0.060396
⊟Function[edit | edit source]
- reaction: EC 2.7.1.56? ExPASy1-phosphofructokinase ATP + D-fructose 1-phosphate = ADP + D-fructose 1,6-bisphosphate
- TIGRFAM: 1-phosphofructokinase (TIGR03828; EC 2.7.1.56; HMM-score: 367.3)hexose kinase, 1-phosphofructokinase family (TIGR03168; EC 2.7.1.-; HMM-score: 361.3)and 6 moreEnergy metabolism Biosynthesis and degradation of polysaccharides tagatose-6-phosphate kinase (TIGR01231; EC 2.7.1.144; HMM-score: 156.7)Energy metabolism Sugars ribokinase (TIGR02152; EC 2.7.1.15; HMM-score: 62.7)Cell envelope Biosynthesis and degradation of surface polysaccharides and lipopolysaccharides bifunctional protein RfaE, domain I (TIGR02198; EC 2.7.1.-; HMM-score: 54)Energy metabolism Sugars 5-dehydro-2-deoxygluconokinase (TIGR04382; EC 2.7.1.92; HMM-score: 48.5)Biosynthesis of cofactors, prosthetic groups, and carriers Pyridoxine pyridoxal kinase (TIGR00687; EC 2.7.1.35; HMM-score: 26.5)Biosynthesis of cofactors, prosthetic groups, and carriers Thiamine hydroxymethylpyrimidine kinase/phosphomethylpyrimidine kinase (TIGR00097; EC 2.7.1.49,2.7.4.7; HMM-score: 24.9)
- TheSEED: see SPN23F07980
- PFAM: Ribokinase (CL0118) PfkB; pfkB family carbohydrate kinase (PF00294; HMM-score: 176.2)and 2 morePhos_pyr_kin; Phosphomethylpyrimidine kinase (PF08543; HMM-score: 30.7)Carb_kinase; Carbohydrate kinase (PF01256; HMM-score: 14.8)
⊟Structure, modifications & cofactors[edit | edit source]
- domains:
- modifications:
- cofactors:
- effectors:
⊟Localization[edit | edit source]
- PSORTb: unknown (no significant prediction)
- Cytoplasmic Score: 2.5
- Cytoplasmic Membrane Score: 2.5
- Cellwall Score: 2.5
- Extracellular Score: 2.5
- Internal Helices: 0
- SignalP: no predicted signal peptide
- SP(Sec/SPI): 0.021715
- TAT(Tat/SPI): 0.000473
- LIPO(Sec/SPII): 0.000966
- predicted transmembrane helices (TMHMM): 0
⊟Accession numbers[edit | edit source]
- GI:
- RefSeq: WP_000640779 NCBI
- UniProt:
⊟Protein sequence[edit | edit source]
- MIYTVTLNPSIDYIVRLDQVKVGSVNRMDSDDKFAGGKGINVSRVLKRLNIPNTATGFIGGFTGKFITDTLAEEEIETRFVQVAEDTRINVKIKADQETEINGTGPTVEPVQLEELKAILSSLTAEDTVVFAGSSAKNLGNVIYKDLISLTRQTGAQVVCDFEGQTLIDSLDYQPLLVKPNNHELGAIFGVKLESLDEIEKYARELLAKGAQNVIISMAGDGALLVTSEGAYFAKPIKGTVKNSVGAGDSMVAGFTGEFVKSKDAVEAFKWGVACGTATTFSDDLATAEFIKETYGKVEVEKR
⊟Experimental data[edit | edit source]
- protein localization:
- interaction partners:
⊟Expression & Regulation[edit | edit source]
⊟Operon[edit | edit source]
⊟Regulation[edit | edit source]
- regulator: FruR* see SPN23F07980
⊟Expression data[edit | edit source]
- PneumoExpress for strain D39V: SPV_0772 PneumoExpress
⊟Biological Material[edit | edit source]
⊟Mutants[edit | edit source]
⊟Expression vector[edit | edit source]
⊟lacZ fusion[edit | edit source]
⊟GFP fusion[edit | edit source]
⊟two-hybrid system[edit | edit source]
⊟FLAG-tag construct[edit | edit source]
⊟Antibody[edit | edit source]
⊟Other Information[edit | edit source]
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