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PangenomeTIGR4
serotype 4
D39
serotype 2
D39V
serotype 2
Hungary19A-6
serotype 19A
EF3030
serotype 19F
670-6B
serotype 6B
6A-10
serotype 6A
70585
serotype 5
A026
serotype 19F
A66
serotype 3
AP200
serotype 11A
ASP0581
serotype 12F
ATCC 49619
serotype 19F
ATCC 700669
serotype 23F
BVJ1JL
serotype 1
CGSP14
serotype 14
G54
serotype 19F
HU-OH
serotype 3
Hu15
serotype 19A
Hu17
serotype 19A
INV104
serotype 1
INV200
serotype 14
JJA
serotype 14
MDRSPN001
serotype 19F
NCTC7466
serotype 2
NU83127
serotype 4
OXC141
serotype 3
P1031
serotype 1
R6
serotype 2
SP49
serotype 19A
SPN032672
serotype 1
SPN034156
serotype 3
SPN034183
serotype 3
SPN994038
serotype 3
SPN994039
serotype 3
SPNA45
serotype 3
ST556
serotype 19F
TCH8431/19A
serotype 19A
Taiwan19F-14
serotype 19F
Xen35
serotype 4
gamPNI0373
serotype 1

NCBI: 26-AUG-2017

⊟Summary[edit | edit source]

  • organism: Streptococcus pneumoniae SPN994038
  • locus tag: SPN994038_08230 [new locus tag: SPN994038_RS04455 ]
  • pan locus tag?: PNEUPAN001937000
  • symbol: proB
  • pan gene symbol?: proB
  • synonym:
  • product: putative glutamate 5-kinase

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SPN994038_08230 [new locus tag: SPN994038_RS04455 ]
  • symbol: proB
  • product: putative glutamate 5-kinase
  • replicon: chromosome
  • strand: +
  • coordinates: 840371..841480
  • length: 1110
  • essential: unknown

⊟Accession numbers[edit | edit source]

  • Location: FQ312041 (840371..841480) NCBI
  • BioCyc:
  • MicrobesOnline:
  • PneumoBrowse for strain D39V: SPV_0822 PneumoBrowse

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    ATGAAATACAAACGGATTGTCTTTAAGGTGGGTACTTCTTCTCTGACAAATGAGGATGGA
    AGTTTATCACGTAGTAAGGTAAAGGATATTACCCAGCAGTTGGCTATGCTGCACGAGGCT
    GGTCATGAGTTGATTTTGGTGTCTTCAGGTGCCATTGCGGCTGGTTTTGGAGCCTTAGGA
    TTTAAAAAGCGTCCGACTAAAATTGCTGATAAACAGGCTTCAGCAGCGGTAGGGCAGGGG
    CTTTTGTTGGAAGAATATACAACCAATCTTCTCTTGCGTCAAATCGTTTCTGCACAAATC
    TTGCTGACCCAAGATGACTTTGTGGATAAGCGTCGTTATAAAAATGCCCATCAGGCTTTG
    TCGGTTTTGCTCAACCGTGGGGCAATTCCTATCATCAATGAGAATGATAGTGTCGTTATT
    GATGAGGTCAAGGTTGGGGACAATGACACTCTAAGTGCCCAAGTAGCGGCGATGGTCCAA
    GCAGACCTTTTGGTTCTCTTGACAGATGTGGACGGTCTCTATACTGGAAATCCTAATTCA
    GATCCAAGAGCCAAACGCTTGGAGAGAATCGAGACCATCAATCGTGAGATTATTGATATG
    GCTGGTGGAGCTGGTTCGTCAAACGGAACTGGGGGTATGTTAACCAAAATCAAGGCTGCA
    ACTATCGCGACGGAATCAGGAGTTCCTGTTTATATCTGCTCATCCTTGAAATCAGATTCC
    ATGATTGAGGCGGCAGAGGAGACCGAGGATGGTTCTTACTTTGTTGCTCAAGAGAAGGGG
    CTTCGTACCCAGAAACAATGGCTTGCCTTCTATGCTCAGAGTCAAGGTTCTATTTGGGTT
    GATAAAGGGGCTGCGGAAGCTCTCTCTCAACATGGAAAGAGTCTTCTCTTATCTGGTATC
    GTTGAAGCAGAAGGAGTCTTTTCTTACGGTGATATCGTGACAGTATTTGACAAGGAAAGT
    GGAAAATCACTTGGAAAAGGACACGTGCAATTTGGAGCATCTGCTTTGGAGGATATGTTG
    CGTTCTCAAAAAGCCAAGGGTGTCTTGATTTACCGTGACGACTGGATTTCCATTACTCCT
    GAAATCCAACTACTTTTTACAGAATTTTAG
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1110

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SPN994038_08230 [new locus tag: SPN994038_RS04455 ]
  • symbol: ProB
  • description: putative glutamate 5-kinase
  • length: 369
  • theoretical pI: 5.29414
  • theoretical MW: 39834
  • GRAVY: -0.0907859

⊟Function[edit | edit source]

  • TIGRFAM:
    Metabolism Amino acid biosynthesis Glutamate family glutamate 5-kinase (TIGR01027; EC 2.7.2.11; HMM-score: 447.8)
    and 10 more
    Metabolism Amino acid biosynthesis Glutamate family delta l-pyrroline-5-carboxylate synthetase (TIGR01092; HMM-score: 155.6)
    Metabolism Purines, pyrimidines, nucleosides, and nucleotides Nucleotide and nucleoside interconversions putative uridylate kinase (TIGR02076; EC 2.7.4.-; HMM-score: 45.8)
    Metabolism Energy metabolism Amino acids and amines carbamate kinase (TIGR00746; EC 2.7.2.2; HMM-score: 37.6)
    Metabolism Purines, pyrimidines, nucleosides, and nucleotides Nucleotide and nucleoside interconversions UMP kinase (TIGR02075; EC 2.7.4.22; HMM-score: 36.4)
    Metabolism Amino acid biosynthesis Glutamate family acetylglutamate kinase (TIGR00761; EC 2.7.2.8; HMM-score: 35.6)
    Metabolism Amino acid biosynthesis Aspartate family aspartate kinase, monofunctional class (TIGR00656; EC 2.7.2.4; HMM-score: 34.1)
    arCOG04150 universal archaeal PUA-domain protein (TIGR03684; HMM-score: 31.6)
    Metabolism Amino acid biosynthesis Aspartate family aspartate kinase (TIGR00657; EC 2.7.2.4; HMM-score: 30.1)
    Metabolism Amino acid biosynthesis Aspartate family putative Pyrococcus aspartate kinase subunit (TIGR02078; EC 2.7.2.4; HMM-score: 22.1)
    uncharacterized domain 2 (TIGR00451; HMM-score: 18)
  • TheSEED: data available for D39, Hungary19A-6, TIGR4
  • PFAM:
    no clan defined AA_kinase; Amino acid kinase family (PF00696; HMM-score: 141.4)
    and 2 more
    PUA (CL0178) PUA; PUA domain (PF01472; HMM-score: 55.7)
    PUA_3; PUA-like domain (PF17785; HMM-score: 13.4)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 9.67
    • Cytoplasmic Membrane Score: 0.01
    • Cellwall Score: 0.15
    • Extracellular Score: 0.17
    • Internal Helices: 0
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.028987
    • TAT(Tat/SPI): 0.005043
    • LIPO(Sec/SPII): 0.002062
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

  • GI:
  • RefSeq: CCP30559 NCBI
  • UniProt:

⊟Protein sequence[edit | edit source]

  • MKYKRIVFKVGTSSLTNEDGSLSRSKVKDITQQLAMLHEAGHELILVSSGAIAAGFGALGFKKRPTKIADKQASAAVGQGLLLEEYTTNLLLRQIVSAQILLTQDDFVDKRRYKNAHQALSVLLNRGAIPIINENDSVVIDEVKVGDNDTLSAQVAAMVQADLLVLLTDVDGLYTGNPNSDPRAKRLERIETINREIIDMAGGAGSSNGTGGMLTKIKAATIATESGVPVYICSSLKSDSMIEAAEETEDGSYFVAQEKGLRTQKQWLAFYAQSQGSIWVDKGAAEALSQHGKSLLLSGIVEAEGVFSYGDIVTVFDKESGKSLGKGHVQFGASALEDMLRSQKAKGVLIYRDDWISITPEIQLLFTEF

⊟Experimental data[edit | edit source]

  • protein localization:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator:

⊟Expression data[edit | edit source]

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

⊟Relevant publications[edit | edit source]

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