PangenomeTIGR4

serotype 4

D39

serotype 2

D39V

serotype 2

Hungary19A-6

serotype 19A

EF3030

serotype 19F

670-6B

serotype 6B

6A-10

serotype 6A

70585

serotype 5

A026

serotype 19F

A66

serotype 3

AP200

serotype 11A

ASP0581

serotype 12F

ATCC 49619

serotype 19F

ATCC 700669

serotype 23F

BM6001

serotype 19F

BVJ1JL

serotype 1

CGSP14

serotype 14

G54

serotype 19F

HU-OH

serotype 3

Hu15

serotype 19A

Hu17

serotype 19A

INV104

serotype 1

INV200

serotype 14

JJA

serotype 14

MDRSPN001

serotype 19F

NCTC7465

serotype 1

NCTC7466

serotype 2

NU83127

serotype 4

OXC141

serotype 3

P1031

serotype 1

R6

serotype 2

SP49

serotype 19A

SPN032672

serotype 1

SPN034156

serotype 3

SPN034183

serotype 3

SPN994038

serotype 3

SPN994039

serotype 3

SPNA45

serotype 3

ST556

serotype 19F

TCH8431/19A

serotype 19A

Taiwan19F-14

serotype 19F

Xen35

serotype 4

gamPNI0373

serotype 1

NCBI: 24-FEB-2025

⊟Summary

• organism: Streptococcus pneumoniae SPN994038
• locus tag: SPN994038_RS08770 [old locus tag: SPN994038_16230 ]
• pan locus tag^?: PNEUPAN003380000
• symbol: sczA
• pan gene symbol^?: sczA
• synonym:
• product: TetR/AcrR family transcriptional regulator SczA

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: SPN994038_RS08770 [old locus tag: SPN994038_16230 ]
• symbol: sczA
• product: TetR/AcrR family transcriptional regulator SczA
• replicon: chromosome
• strand: +
• coordinates: 1643949..1644485
• length: 537
• essential: unknown

⊟Accession numbers

• Location: NC_021026 (1643949..1644485) NCBI
• BioCyc:
• MicrobesOnline:
• PneumoBrowse for strain D39V: SPV_1639 PneumoBrowse

⊟Phenotype

Share your knowledge and add information here. [edit]

⊟DNA sequence

• 1
  61
  121
  181
  241
  301
  361
  421
  481
  
  ATGACTAACATTGACCGCCGTATCAGCAAAACCAAAAAAGCCATCTATCAAGCTTTTATA
  CAACTTTTGAATGCTAAGGGCTACGAGGCCACTACTGTTCAGGATATCATTGATCTCGCA
  GATGTGGGACGATCCACCTTTTACTGTCACTATGAGAGTAAGGAGCTTCTTCTGGACCAG
  CTCTGCCGCTACCTCTTCCATCATCTTTTTGAAAGAGAGCAAGCCATTTCAACCGAGGAC
  TACCTCGCCCACCTCTTTCTCCATTTTCATAAAAACCAAGACCATATCACCAGTCTGCTA
  TTTTCCAAAAATGACTACTTCCTCCGTCAACTCCATAAAGAGCTAGAACACCATGTCTAT
  TCCGTGCTAGCTGATAATTTAAAAGAAGCCCACCCTAATCTGCCTACTTCTTACCTCCAA
  CACTTGGTCGTGTCCAACTTTATCGAGACATTGACCTGGTGGCTCAAAAAAGGTCAAGAT
  TTCACAGACCAGGAAGTTGTCCAATTTTATCTAGACCTTCTCATTCCTAAAAATTGA
  60
  120
  180
  240
  300
  360
  420
  480
  537
  

⊟Protein

⊟General

• locus tag: SPN994038_RS08770 [old locus tag: SPN994038_16230 ]
• symbol: SczA
• description: TetR/AcrR family transcriptional regulator SczA
• length: 178
• theoretical pI: 6.64763
• theoretical MW: 21201
• GRAVY: -0.335955

⊟Function

• TIGRFAM:
  probable dihydroxyacetone kinase regulator (TIGR02366; HMM-score: 46.4)
  and 4 more
  Signal transduction Regulatory functions DNA interactions mycofactocin system transcriptional regulator (TIGR03968; HMM-score: 34.9)
  Signal transduction Regulatory functions DNA interactions transcriptional repressor BetI (TIGR03384; HMM-score: 20.8)
  pyrimidine utilization regulatory protein R (TIGR03613; HMM-score: 19.1)
  Metabolism Energy metabolism Biosynthesis and degradation of polysaccharides beta-phosphoglucomutase (TIGR01990; EC 5.4.2.6; HMM-score: 13)
• TheSEED: data available for D39, Hungary19A-6, TIGR4
• PFAM:
  HTH (CL0123) TetR_N; Bacterial regulatory proteins, tetR family (PF00440; HMM-score: 41.4)
  TetR_C (CL0174) TetR_C_8; Transcriptional regulator C-terminal region (PF14278; HMM-score: 34.9)
  and 3 more
  no clan defined ER; Enhancer of rudimentary (PF01133; HMM-score: 13.8)
  TetR_C (CL0174) TetR_C_30; Tetracyclin repressor-like, C-terminal domain (PF17939; HMM-score: 13.1)
  HTH (CL0123) DUF6262; Family of unknown function (DUF6262) (PF19776; HMM-score: 12.6)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:
• genes regulated by 

  SczA	TF important in Zinc resistance:	in D39V
  SczA*	TF important in Zinc resistance:	in TIGR4

⊟Localization

• PSORTb: unknown (no significant prediction)
  • Cytoplasmic Score: 2.5
  • Cytoplasmic Membrane Score: 2.5
  • Cellwall Score: 2.5
  • Extracellular Score: 2.5
  • Internal Helices: 0
• DeepLocPro: Cytoplasmic
  • Cytoplasmic Score: 0.9704
  • Cytoplasmic Membrane Score: 0.0264
  • Cell wall & surface Score: 0.0003
  • Extracellular Score: 0.0029
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.024222
  • TAT(Tat/SPI): 0.001436
  • LIPO(Sec/SPII): 0.008789
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI:
• RefSeq: WP_000181690 NCBI
• UniProt:

⊟Protein sequence

• MTNIDRRISKTKKAIYQAFIQLLNAKGYEATTVQDIIDLADVGRSTFYCHYESKELLLDQLCRYLFHHLFEREQAISTEDYLAHLFLHFHKNQDHITSLLFSKNDYFLRQLHKELEHHVYSVLADNLKEAHPNLPTSYLQHLVVSNFIETLTWWLKKGQDFTDQEVVQFYLDLLIPKN

⊟Experimental data

• protein localization:
• interaction partners:

⊟Expression & Regulation

⊟Operon

⊟Regulation

• data available for D39V, TIGR4

⊟Transcription

• transcription start site:

⊟Expression data

• PneumoExpress for strain D39V: SPV_1639 PneumoExpress

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You can add further information about the gene and protein here. [edit]

⊟Literature

⊟References

⊟Relevant publications

Tomas G Kloosterman, Magdalena M van der Kooi-Pol, Jetta J E Bijlsma, Oscar P Kuipers
The novel transcriptional regulator SczA mediates protection against Zn2+ stress by activation of the Zn2+-resistance gene czcD in Streptococcus pneumoniae.
Mol Microbiol: 2007, 65(4);1049-63
[PubMed:17640279] [WorldCat.org] [DOI] (P p)Julia E Martin, Katherine A Edmonds, Kevin E Bruce, Gregory C Campanello, Bart A Eijkelkamp, Erin B Brazel, Christopher A McDevitt, Malcolm E Winkler, David P Giedroc
The zinc efflux activator SczA protects Streptococcus pneumoniae serotype 2 D39 from intracellular zinc toxicity.
Mol Microbiol: 2017, 104(4);636-651
[PubMed:28249108] [WorldCat.org] [DOI] (I p)