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PangenomeTIGR4
serotype 4
D39
serotype 2
D39V
serotype 2
Hungary19A-6
serotype 19A
EF3030
serotype 19F
670-6B
serotype 6B
6A-10
serotype 6A
70585
serotype 5
A026
serotype 19F
A66
serotype 3
AP200
serotype 11A
ASP0581
serotype 12F
ATCC 49619
serotype 19F
ATCC 700669
serotype 23F
BM6001
serotype 19F
BVJ1JL
serotype 1
CGSP14
serotype 14
G54
serotype 19F
HU-OH
serotype 3
Hu15
serotype 19A
Hu17
serotype 19A
INV104
serotype 1
INV200
serotype 14
JJA
serotype 14
MDRSPN001
serotype 19F
NCTC7465
serotype 1
NCTC7466
serotype 2
NU83127
serotype 4
OXC141
serotype 3
P1031
serotype 1
R6
serotype 2
SP49
serotype 19A
SPN032672
serotype 1
SPN034156
serotype 3
SPN034183
serotype 3
SPN994038
serotype 3
SPN994039
serotype 3
SPNA45
serotype 3
ST556
serotype 19F
TCH8431/19A
serotype 19A
Taiwan19F-14
serotype 19F
Xen35
serotype 4
gamPNI0373
serotype 1

NCBI: 05-APR-2025

⊟Summary[edit | edit source]

  • organism: Streptococcus pneumoniae SPN994039
  • locus tag: SPN994039_RS05435 [old locus tag: SPN994039_10140 ]
  • pan locus tag?: PNEUPAN002257000
  • symbol: pulA
  • pan gene symbol?: pulA
  • synonym:
  • product: type I pullulanase

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SPN994039_RS05435 [old locus tag: SPN994039_10140 ]
  • symbol: pulA
  • product: type I pullulanase
  • replicon: chromosome
  • strand: +
  • coordinates: 1019921..1022200
  • length: 2280
  • essential: unknown

⊟Accession numbers[edit | edit source]

  • Location: NC_021005 (1019921..1022200) NCBI
  • BioCyc:
  • MicrobesOnline:
  • PneumoBrowse for strain D39V: SPV_1002 PneumoBrowse

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    ATGTATAACTACCCTATGCGCATTCATTACCATCGTAAGAATGGAGAATATGACACTTGC
    TCCTTTGTAAAAAGTCAGGATCAACGAATTGATTTGCTAACCTACAAAGAAGATTATTTT
    GGAGCTTTATTTAGCTTTGAACACCCAAGTTCACATGTTATAGAAAGCTTGAATTTTGTG
    GTTCATACAGGTCAAACTAGTAAAGAATATTCTATCCGTTTTAATCACTATCCCCTATTA
    ACAGAGGTCTGGATTTTGGAAGGCGATGATAGGATTTATTACTCTGAAAATCCTGCTATT
    GCCAGTCCTTTCTATAAAAATCAAAATCCTTTTGCCTTTGATAAGGCCATTAACAGTGCT
    AGTTTTGATCATCATTGGGGTTACCAAGGAGAATTGGGTTGCCGTGTAGAGGACAATCAG
    GCTCATTTTTCCCTCTGGTCACCTACAGCGACAGAAGTGCAAGTTGTCGTTTATGAATCA
    GCTGCTAATGATGCACCCGTTTGGAAGACTTTTGAGATGAAAAGAGGCAATAGCTACTCT
    TATAATCATAAGGACAATACAATCGGTGTCTGGAGTTTGGATGTTGAAGAAGATTTGGTA
    GGTAAGACTTATCAGTATCAAGTCCAATTCCCTCATCACCAAACACTGACACGTGATCCT
    TATACAATCGCGACCAGCCCTGATGGCAAACGTTCAGCTATTCTGAGCCATGTAGAAAAG
    CAAGTTGAAAACTTCGAGGTTAAGCACGGTTCGGAGGCTACTTGGCGCTTGGAAAATCCA
    TGTAAGGCAGTTATCTGTGAAATGCACATTCGTGATTTGACTAAATCACCTACATCGGGT
    GTAGATGAACATCTTCGAGGAACTTTCTTGGGTGCTGCTCAGGCTGGAACAGTTAACCAA
    TACGGTCAGTCAACTGCTTTTGATTACATCAAGAAGCTGGGCTACAATTATGTTCAATTG
    CAACCAATTGCAGACCGTCATAAAGAATACGATGAGGATGGAAATGTAACCTACAACTGG
    GGTTATGATCCACAAAACTATAACGCGCCAGAAACTAGTTTTTCAACTAATCCAGATGAT
    CCAGCTCAGGTCATTCGTGATTTGAAGGTGATGGTTCAAGCTTATCACGATGCGGGTATT
    GGAGTCATTATGGATGTAGTCTATAACCATACCTTCTCAGTTGTTGATGCACCATTCCAA
    ACAACAGTCCCTGATTACTATTATCGTATGAATCCAGATGGTACCTTCCAGAATGGAACG
    GGTGTTGGAAATGAAACAGCCAGTGAACACGAAATGTTTCGCAAGTATATGATTGATTCT
    CTTCTATACTGGGTGCAGGAATATAATATTGACGGCTTCCGTTTTGACTTGATGGGGATT
    CATGATGTCAAGACCATGCAGATGATTCGTCAAAGCTTGGATGAAATCGACTCCAACATT
    ATCCTCTATGGAGAAGGATGGGATATGGGAACAGGTCTTACCCCTTATGATAAGGCCAAG
    AAGGACAATGCCTACCAGATGCCAAATATTGGTTTCTTTAATGACAATCAGCGCGATGCT
    GTCAAAGGGGGAGAAGTTTATGGTGCTATCAAGTCAGGTTTTGTCAGTGGTGCTGCGACA
    GAGCCAATTCTAGCTAAAGCAATCCTAGGAAGTCGTGAATTAGGAACCTATACACATCCA
    AATCAGGTGCTTAACTATGTAGAAGCCCATGACAATTACAATCTTCACGATTTATTGGCA
    ACCCTTCATCCAGACCAAAGTTCAGAGCAAATCATGCGCAAGGTCGAAACTGCCACAGCC
    ATGAATCTGCTCATGCAGGGGATGGCCTTTATGGAAATCGGTCAAGAATTTGGTCGTACC
    AAACTGGTTGCGACTGGTGAAAATGGTGAGTTGACCCATGATGATAGAGAGCGTGCGATG
    AATAGCTATAATGCTCCTGACAGTGTGAACCAAGTGAACTGGAACTTGATTAATGAGCGT
    CAAGACAGTATTGAGTTTATCCGTCAAGTCATCCGATTGAAGACAAAAACTGTTGCTTTT
    TCTTACTCTAGCTATGATGAAATTTACCATCATGTCTTTGTGCATTCTGCGATTGAACAT
    AGCGGCTGCCTTATCTATGAAGTTCACGGCAAAGAACACCTCTTGGTAGTTGTGAATGCT
    AAATCAGAACCCTATCAATTTGAAAATGCAGGAAATTTAGCTATGTTGGTAACCAACAGT
    CGCTCAAAAGAAGATAATGTTTTAAATGATATTAGTCTAGCTGTCTTGAGTGTTTTATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1320
    1380
    1440
    1500
    1560
    1620
    1680
    1740
    1800
    1860
    1920
    1980
    2040
    2100
    2160
    2220
    2280

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SPN994039_RS05435 [old locus tag: SPN994039_10140 ]
  • symbol: PulA
  • description: type I pullulanase
  • length: 759
  • theoretical pI: 5.02367
  • theoretical MW: 86602.7
  • GRAVY: -0.525165

⊟Function[edit | edit source]

  • reaction:
    EC 3.2.1.41?  ExPASy
    Pullulanase Hydrolysis of (1->6)-alpha-D-glucosidic linkages in pullulan, amylopectin and glycogen, and in the alpha- and beta-limit dextrins of amylopectin and glycogen
  • TIGRFAM:
    pullulanase, type I (TIGR02104; EC 3.2.1.41; HMM-score: 817)
    and 7 more
    Metabolism Energy metabolism Biosynthesis and degradation of polysaccharides alpha-1,6-glucosidases, pullulanase-type (TIGR02103; EC 3.2.1.41; HMM-score: 278.1)
    pullulanase, extracellular (TIGR02102; HMM-score: 272.3)
    Metabolism Energy metabolism Biosynthesis and degradation of polysaccharides glycogen debranching enzyme GlgX (TIGR02100; EC 3.2.1.-; HMM-score: 160.4)
    Metabolism Energy metabolism Biosynthesis and degradation of polysaccharides malto-oligosyltrehalose trehalohydrolase (TIGR02402; EC 3.2.1.141; HMM-score: 73.4)
    Metabolism Energy metabolism Biosynthesis and degradation of polysaccharides 1,4-alpha-glucan branching enzyme (TIGR01515; EC 2.4.1.18; HMM-score: 62.6)
    alpha,alpha-phosphotrehalase (TIGR02403; EC 3.2.1.93; HMM-score: 21.1)
    Metabolism Energy metabolism Biosynthesis and degradation of polysaccharides trehalose synthase (TIGR02456; EC 5.4.99.16; HMM-score: 15.1)
  • TheSEED: data available for D39, Hungary19A-6, TIGR4
  • PFAM:
    Glyco_hydro_tim (CL0058) Alpha-amylase; Alpha amylase, catalytic domain (PF00128; HMM-score: 51.1)
    GHD (CL0369) CBM_48; Carbohydrate-binding module 48 (Isoamylase N-terminal domain) (PF02922; HMM-score: 42.8)
    and 2 more
    Transthyretin (CL0287) PUD; Bacterial pullanase-associated domain (PF03714; HMM-score: 25.8)
    Glyco_hydro_tim (CL0058) hDGE_amylase; Glycogen debranching enzyme, glucanotransferase domain (PF14701; HMM-score: 14.3)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.003992
    • TAT(Tat/SPI): 0.000128
    • LIPO(Sec/SPII): 0.00062
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

  • GI:
  • RefSeq: WP_000283076 NCBI
  • UniProt:

⊟Protein sequence[edit | edit source]

  • MYNYPMRIHYHRKNGEYDTCSFVKSQDQRIDLLTYKEDYFGALFSFEHPSSHVIESLNFVVHTGQTSKEYSIRFNHYPLLTEVWILEGDDRIYYSENPAIASPFYKNQNPFAFDKAINSASFDHHWGYQGELGCRVEDNQAHFSLWSPTATEVQVVVYESAANDAPVWKTFEMKRGNSYSYNHKDNTIGVWSLDVEEDLVGKTYQYQVQFPHHQTLTRDPYTIATSPDGKRSAILSHVEKQVENFEVKHGSEATWRLENPCKAVICEMHIRDLTKSPTSGVDEHLRGTFLGAAQAGTVNQYGQSTAFDYIKKLGYNYVQLQPIADRHKEYDEDGNVTYNWGYDPQNYNAPETSFSTNPDDPAQVIRDLKVMVQAYHDAGIGVIMDVVYNHTFSVVDAPFQTTVPDYYYRMNPDGTFQNGTGVGNETASEHEMFRKYMIDSLLYWVQEYNIDGFRFDLMGIHDVKTMQMIRQSLDEIDSNIILYGEGWDMGTGLTPYDKAKKDNAYQMPNIGFFNDNQRDAVKGGEVYGAIKSGFVSGAATEPILAKAILGSRELGTYTHPNQVLNYVEAHDNYNLHDLLATLHPDQSSEQIMRKVETATAMNLLMQGMAFMEIGQEFGRTKLVATGENGELTHDDRERAMNSYNAPDSVNQVNWNLINERQDSIEFIRQVIRLKTKTVAFSYSSYDEIYHHVFVHSAIEHSGCLIYEVHGKEHLLVVVNAKSEPYQFENAGNLAMLVTNSRSKEDNVLNDISLAVLSVL

⊟Experimental data[edit | edit source]

  • protein localization:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator:

⊟Expression data[edit | edit source]

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

⊟Relevant publications[edit | edit source]

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