From PneumoWiki
Jump to navigation Jump to search
PangenomeTIGR4
serotype 4
D39
serotype 2
D39V
serotype 2
Hungary19A-6
serotype 19A
EF3030
serotype 19F
670-6B
serotype 6B
6A-10
serotype 6A
70585
serotype 5
A026
serotype 19F
A66
serotype 3
AP200
serotype 11A
ASP0581
serotype 12F
ATCC 49619
serotype 19F
ATCC 700669
serotype 23F
BVJ1JL
serotype 1
CGSP14
serotype 14
G54
serotype 19F
HU-OH
serotype 3
Hu15
serotype 19A
Hu17
serotype 19A
INV104
serotype 1
INV200
serotype 14
JJA
serotype 14
MDRSPN001
serotype 19F
NCTC7466
serotype 2
NU83127
serotype 4
OXC141
serotype 3
P1031
serotype 1
R6
serotype 2
SP49
serotype 19A
SPN032672
serotype 1
SPN034156
serotype 3
SPN034183
serotype 3
SPN994038
serotype 3
SPN994039
serotype 3
SPNA45
serotype 3
ST556
serotype 19F
TCH8431/19A
serotype 19A
Taiwan19F-14
serotype 19F
Xen35
serotype 4
gamPNI0373
serotype 1

NCBI: 13-DEC-2020

⊟Summary[edit | edit source]

  • organism: Streptococcus pneumoniae SPN994039
  • locus tag: SPN994039_RS07545 [old locus tag: SPN994039_13990 ]
  • pan locus tag?: PNEUPAN002991000
  • symbol: galE
  • pan gene symbol?: galE-1
  • synonym:
  • product: UDP-glucose 4-epimerase GalE

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SPN994039_RS07545 [old locus tag: SPN994039_13990 ]
  • symbol: galE
  • product: UDP-glucose 4-epimerase GalE
  • replicon: chromosome
  • strand: -
  • coordinates: 1418433..1419452
  • length: 1020
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

  • Location: NC_021005 (1418433..1419452) NCBI
  • BioCyc:
  • MicrobesOnline:
  • PneumoBrowse for strain D39V: SPV_1432 PneumoBrowse

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    ATGCAAGAAAAGATTTTGGTAACTGGTGGTGCCGGTTTTATTGGAACCCACACTGTTATT
    GAATTGATCCAAGCAGGCCATCAAGTTGTTGTGGTGGATAATCTTGTCAATAGCAATCGT
    AAGAGTTTAGAAGTTGTTGAAAGAATCACAGGAGTTGAGATTCCTTTCTATGAGGCAGAT
    ATTCGTGACACAGATACCCTCAGAGATATTTTCAAGCAGGAAGAACTGACTGGTGTCATT
    CACTTTGCTGGTTTGAAGGCTGTTGGCGAATCAACACGTATCCCTCTTGCCTACTATGAC
    AACAATATCGCTGGAACTGTCAGCCTTTTGAAAGCCATGGAAGAAAACAACTGTAAAAAC
    ATCATCTTCAGTTCTTCTGCGACAGTTTACGGGGATCCGTACACAGTGCCCATCTTGGAA
    GATTTCCCACTTTCAGTGACCAACCCATACGGTCGTACCAAGCTCATGCTAGAGGAAATT
    TTGACCGATATTTACAAAGCAGACTCAGAATGGAATGTTGTCTTGCTTCGTTACTTTAAC
    CCAATCGGAGCCCATGAGAGTGGTGATTTGGGAGAAAATCCAAACGGTATTCCAAACAAT
    CTCTTGCCATATGTGACTCAAGTAGCCGTTGGAAAATTAGAGCAAGTGCAAGTGTTTGGA
    GACGATTACGATACGGAAGATGGAACAGGTGTTCGTGACTATATCCACGTTGTCGATTTG
    GCTAAGGGTCACGTTGCAGCTTTGAAAAAAATCCAAAAAGGTTCAGGACTAAACGTTTAT
    AACCTTGGAACTGGTAAAGGTTACTCAGTTCTTGAAATTATCCAAAACATGGAAAAAGCG
    GTGGGACGTCCTATTCCTTACCGCATCGTAGAACGTCGCCCAGGTGATATCGCTGCCTGC
    TACTCAGACCCGGCAAAAGCTAAAGCAGAACTCGGTTGGGAAGCAGAACTCGACATCACC
    CAAATGTGTGAAGACGCATGGCGTTGGCAAAGCAAGCATCCAAATGGATTTGAAGACTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SPN994039_RS07545 [old locus tag: SPN994039_13990 ]
  • symbol: GalE
  • description: UDP-glucose 4-epimerase GalE
  • length: 339
  • theoretical pI: 4.55593
  • theoretical MW: 37520.2
  • GRAVY: -0.218289

⊟Function[edit | edit source]

  • reaction:
    EC 5.1.3.2?  ExPASy
    UDP-glucose 4-epimerase UDP-alpha-D-glucose = UDP-alpha-D-galactose
  • TIGRFAM:
    Metabolism Energy metabolism Sugars UDP-glucose 4-epimerase GalE (TIGR01179; EC 5.1.3.2; HMM-score: 468.4)
    and 22 more
    Cell structure Cell envelope Biosynthesis and degradation of surface polysaccharides and lipopolysaccharides dTDP-glucose 4,6-dehydratase (TIGR01181; EC 4.2.1.46; HMM-score: 119.2)
    NAD dependent epimerase/dehydratase, LLPSF_EDH_00030 family (TIGR04180; HMM-score: 110.2)
    Cell structure Cell envelope Biosynthesis and degradation of surface polysaccharides and lipopolysaccharides ADP-glyceromanno-heptose 6-epimerase (TIGR02197; EC 5.1.3.20; HMM-score: 109.3)
    hopanoid-associated sugar epimerase (TIGR03466; HMM-score: 67.3)
    Cell structure Cell envelope Biosynthesis and degradation of surface polysaccharides and lipopolysaccharides CDP-glucose 4,6-dehydratase (TIGR02622; EC 4.2.1.45; HMM-score: 63.5)
    UDP-N-acetylglucosamine 4,6-dehydratase (inverting) (TIGR03589; EC 4.2.1.115; HMM-score: 34.9)
    Hypothetical proteins Conserved TIGR01777 family protein (TIGR01777; HMM-score: 31.3)
    Cell structure Cell envelope Biosynthesis and degradation of surface polysaccharides and lipopolysaccharides GDP-mannose 4,6-dehydratase (TIGR01472; EC 4.2.1.47; HMM-score: 30.5)
    thioester reductase domain (TIGR01746; HMM-score: 26.9)
    Cell structure Cell envelope Biosynthesis and degradation of surface polysaccharides and lipopolysaccharides dTDP-4-dehydrorhamnose reductase (TIGR01214; EC 1.1.1.133; HMM-score: 26.7)
    2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase (TIGR04316; EC 1.3.1.28; HMM-score: 22.9)
    acetoacetyl-CoA reductase (TIGR01829; EC 1.1.1.36; HMM-score: 20.7)
    UDP-N-acetylglucosamine 4,6-dehydratase/5-epimerase (TIGR04130; EC 4.2.1.-,5.1.3.-; HMM-score: 19.5)
    3-hydroxybutyrate dehydrogenase (TIGR01963; HMM-score: 18.8)
    Metabolism Fatty acid and phospholipid metabolism Biosynthesis 3-oxoacyl-[acyl-carrier-protein] reductase (TIGR01830; EC 1.1.1.100; HMM-score: 18.6)
    Metabolism Energy metabolism Fermentation acetoin reductases (TIGR02415; EC 1.1.1.-; HMM-score: 18.6)
    Unknown function Enzymes of unknown specificity SDR family mycofactocin-dependent oxidoreductase (TIGR04504; EC 1.1.99.-; HMM-score: 17.8)
    rhamnulose-1-phosphate aldolase/alcohol dehydrogenase (TIGR02632; EC 1.1.1.1,4.1.2.19; HMM-score: 17.5)
    polyketide-type polyunsaturated fatty acid synthase PfaA (TIGR02813; HMM-score: 16.1)
    nucleotide sugar dehydrogenase (TIGR03026; HMM-score: 15.6)
    2-hydroxycyclohexanecarboxyl-CoA dehydrogenase (TIGR03206; EC 1.1.1.-; HMM-score: 14.2)
    Metabolism Energy metabolism Biosynthesis and degradation of polysaccharides 2-deoxy-D-gluconate 3-dehydrogenase (TIGR01832; EC 1.1.1.125; HMM-score: 12.4)
  • TheSEED: data available for D39, Hungary19A-6, TIGR4
  • PFAM:
    NADP_Rossmann (CL0063) GDP_Man_Dehyd; GDP-mannose 4,6 dehydratase (PF16363; HMM-score: 208.1)
    Epimerase; NAD dependent epimerase/dehydratase family (PF01370; HMM-score: 188.3)
    and 13 more
    3Beta_HSD; 3-beta hydroxysteroid dehydrogenase/isomerase family (PF01073; HMM-score: 65.4)
    Polysacc_synt_2; Polysaccharide biosynthesis protein (PF02719; HMM-score: 52)
    RmlD_sub_bind; RmlD substrate binding domain (PF04321; HMM-score: 38.3)
    KR; KR domain (PF08659; HMM-score: 37.3)
    adh_short; short chain dehydrogenase (PF00106; HMM-score: 31.8)
    NAD_binding_10; NAD(P)H-binding (PF13460; HMM-score: 30.2)
    NmrA; NmrA-like family (PF05368; HMM-score: 24)
    NAD_binding_4; Male sterility protein (PF07993; HMM-score: 23.7)
    adh_short_C2; Enoyl-(Acyl carrier protein) reductase (PF13561; HMM-score: 17.3)
    UDPG_MGDP_dh_N; UDP-glucose/GDP-mannose dehydrogenase family, NAD binding domain (PF03721; HMM-score: 17)
    TrkA_N; TrkA-N domain (PF02254; HMM-score: 15.3)
    NAD_binding_7; Putative NAD(P)-binding (PF13241; HMM-score: 12.8)
    PELOTA (CL0101) SpoU_sub_bind; RNA 2'-O ribose methyltransferase substrate binding (PF08032; HMM-score: 12.4)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.024002
    • TAT(Tat/SPI): 0.001156
    • LIPO(Sec/SPII): 0.001695
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

  • GI:
  • RefSeq: WP_001156527 NCBI
  • UniProt:

⊟Protein sequence[edit | edit source]

  • MQEKILVTGGAGFIGTHTVIELIQAGHQVVVVDNLVNSNRKSLEVVERITGVEIPFYEADIRDTDTLRDIFKQEELTGVIHFAGLKAVGESTRIPLAYYDNNIAGTVSLLKAMEENNCKNIIFSSSATVYGDPYTVPILEDFPLSVTNPYGRTKLMLEEILTDIYKADSEWNVVLLRYFNPIGAHESGDLGENPNGIPNNLLPYVTQVAVGKLEQVQVFGDDYDTEDGTGVRDYIHVVDLAKGHVAALKKIQKGSGLNVYNLGTGKGYSVLEIIQNMEKAVGRPIPYRIVERRPGDIAACYSDPAKAKAELGWEAELDITQMCEDAWRWQSKHPNGFED

⊟Experimental data[edit | edit source]

  • protein localization:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator:

⊟Expression data[edit | edit source]

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

⊟Relevant publications[edit | edit source]

Retrieved from "http://pneumowiki.med.uni-greifswald.de/index.php?title=SPN994039_RS07545&oldid=151234"