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PangenomeTIGR4
serotype 4
D39
serotype 2
D39V
serotype 2
Hungary19A-6
serotype 19A
EF3030
serotype 19F
670-6B
serotype 6B
6A-10
serotype 6A
70585
serotype 5
A026
serotype 19F
A66
serotype 3
AP200
serotype 11A
ASP0581
serotype 12F
ATCC 49619
serotype 19F
ATCC 700669
serotype 23F
BVJ1JL
serotype 1
CGSP14
serotype 14
G54
serotype 19F
HU-OH
serotype 3
Hu15
serotype 19A
Hu17
serotype 19A
INV104
serotype 1
INV200
serotype 14
JJA
serotype 14
MDRSPN001
serotype 19F
NCTC7466
serotype 2
NU83127
serotype 4
OXC141
serotype 3
P1031
serotype 1
R6
serotype 2
SP49
serotype 19A
SPN032672
serotype 1
SPN034156
serotype 3
SPN034183
serotype 3
SPN994038
serotype 3
SPN994039
serotype 3
SPNA45
serotype 3
ST556
serotype 19F
TCH8431/19A
serotype 19A
Taiwan19F-14
serotype 19F
Xen35
serotype 4
gamPNI0373
serotype 1

NCBI: 13-DEC-2020

⊟Summary[edit | edit source]

  • organism: Streptococcus pneumoniae SPNA45
  • locus tag: SPNA45_RS03715 [old locus tag: SPNA45_00644 ]
  • pan locus tag?: PNEUPAN002962000
  • symbol: SPNA45_RS03715
  • pan gene symbol?: rebM
  • synonym:
  • product: class I SAM-dependent methyltransferase

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SPNA45_RS03715 [old locus tag: SPNA45_00644 ]
  • symbol: SPNA45_RS03715
  • product: class I SAM-dependent methyltransferase
  • replicon: chromosome
  • strand: +
  • coordinates: 645073..645831
  • length: 759
  • essential: unknown

⊟Accession numbers[edit | edit source]

  • Location: NC_018594 (645073..645831) NCBI
  • BioCyc: SPNA45_RS03715 BioCyc
  • MicrobesOnline:
  • PneumoBrowse for strain D39V: SPV_1408 PneumoBrowse

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    ATGTCAGAAGCAGGTCATAAGTTTTTAGCAAAATTGGGGAAAAAACGCTTACGTCCAGGT
    GGAAAGCGTGCCACAGATTGGTTAATTGCAGAAGGAGGATTTTCAAAAGAAAAGAGAATA
    CTAGAGGTTGCGTGTAATAGGGGAACTACAGCAATTAAGTTGGCACAGCGTTTTTGTTGC
    AAGATAACTGCTGTTGATATGGATGCTCAAGCTTTAGAAGTGGCTAAAAAATCTGCTGGA
    ACGGCAGGTGTTGCTCATTTAATCAGTTTTGAAAGAGCAAATGCAATGAAACTTCCTTAT
    CAAGATGCTAGTTTTGATATTGTTATAAATGAAGCTATGCTGACTATGCAAGCCGATCAA
    GCTAAGAAAAAATGTGTAATGGAATATCTAAGGGTATTAAAACCTGGAGGTCTTCTCTTG
    ACACATGATGTGCTTCTTAAGGAAGCTAAAGAGTCTATCAGACAGGAATTATCACAAGCA
    ATTCATGTAAATGTAGGTCCTTTAACTCAAGATGGTTGGGAACAGGTGATGATAGAATCA
    GGTTATTGTGATGTGAAAGTATTGACTGGTGAAATGACATTAATGAAATTATCGGGTATG
    ATTTATGACGAAGGTTGGCTAGGAACTTTGAAAATTTGTGTAAATGCTTGTAAAAAGGAG
    AATAGAAAGCAGTTTTTAACTATGTATAAAATGTTTGCTAAGAATAAACAGAAATTGGGC
    TTTATTGCGATGGCTAGTTATAAATCGTCAAAACGTTAG
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    759

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SPNA45_RS03715 [old locus tag: SPNA45_00644 ]
  • symbol: SPNA45_RS03715
  • description: class I SAM-dependent methyltransferase
  • length: 252
  • theoretical pI: 10.0368
  • theoretical MW: 28077
  • GRAVY: -0.191667

⊟Function[edit | edit source]

  • TIGRFAM:
    Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Menaquinone and ubiquinone ubiquinone/menaquinone biosynthesis methyltransferase (TIGR01934; EC 2.1.1.-; HMM-score: 56.4)
    and 15 more
    Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Menaquinone and ubiquinone demethylmenaquinone methyltransferase (TIGR02752; EC 2.1.1.163; HMM-score: 38.3)
    Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Biotin malonyl-acyl carrier protein O-methyltransferase BioC (TIGR02072; EC 2.1.1.-; HMM-score: 38.1)
    Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Menaquinone and ubiquinone 3-demethylubiquinone-9 3-O-methyltransferase (TIGR01983; EC 2.1.1.64; HMM-score: 35.9)
    methyltransferase, Rta_06860 family (TIGR04290; EC 2.1.1.-; HMM-score: 31)
    Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Glutathione and analogs putative 4-mercaptohistidine N1-methyltranferase (TIGR04345; HMM-score: 30)
    Genetic information processing Protein fate Protein modification and repair methyltransferase, HemK family (TIGR00536; HMM-score: 27.4)
    Genetic information processing Protein synthesis Ribosomal proteins: synthesis and modification ribosomal protein L11 methyltransferase (TIGR00406; EC 2.1.1.-; HMM-score: 26.6)
    Genetic information processing Protein fate Protein modification and repair protein-(glutamine-N5) methyltransferase, release factor-specific (TIGR03534; EC 2.1.1.-; HMM-score: 24.8)
    Genetic information processing Protein synthesis Ribosomal proteins: synthesis and modification protein-(glutamine-N5) methyltransferase, ribosomal protein L3-specific (TIGR03533; EC 2.1.1.-; HMM-score: 23)
    Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Chlorophyll and bacteriochlorphyll magnesium protoporphyrin O-methyltransferase (TIGR02021; EC 2.1.1.11; HMM-score: 20.6)
    Unknown function Enzymes of unknown specificity putative methylase (TIGR00537; HMM-score: 16.1)
    Metabolism Amino acid biosynthesis Aspartate family methionine biosynthesis protein MetW (TIGR02081; HMM-score: 14.6)
    2-ketoarginine methyltransferase (TIGR04543; EC 2.1.1.243; HMM-score: 13.7)
    Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Heme, porphyrin, and cobalamin precorrin-6Y C5,15-methyltransferase (decarboxylating), CbiT subunit (TIGR02469; EC 2.1.1.132; HMM-score: 13.3)
    pseudaminic acid biosynthesis-associated methylase (TIGR03587; HMM-score: 13.1)
  • TheSEED: data available for D39, Hungary19A-6, TIGR4
  • PFAM:
    NADP_Rossmann (CL0063) Methyltransf_25; Methyltransferase domain (PF13649; HMM-score: 70)
    Methyltransf_31; Methyltransferase domain (PF13847; HMM-score: 69.5)
    Methyltransf_11; Methyltransferase domain (PF08241; HMM-score: 67.1)
    and 20 more
    Ubie_methyltran; ubiE/COQ5 methyltransferase family (PF01209; HMM-score: 49.7)
    Methyltransf_23; Methyltransferase domain (PF13489; HMM-score: 42.1)
    Methyltransf_3; O-methyltransferase (PF01596; HMM-score: 29.3)
    UPF0020; Putative RNA methylase family UPF0020 (PF01170; HMM-score: 29)
    Methyltransf_12; Methyltransferase domain (PF08242; HMM-score: 28)
    MTS; Methyltransferase small domain (PF05175; HMM-score: 26.5)
    CheR; CheR methyltransferase, SAM binding domain (PF01739; HMM-score: 24.6)
    PrmA; Ribosomal protein L11 methyltransferase (PrmA) (PF06325; HMM-score: 22.1)
    CMAS; Mycolic acid cyclopropane synthetase (PF02353; HMM-score: 20.7)
    Methyltransf_24; Methyltransferase domain (PF13578; HMM-score: 18.7)
    ADH_zinc_N; Zinc-binding dehydrogenase (PF00107; HMM-score: 18)
    NodS; Nodulation protein S (NodS) (PF05401; HMM-score: 17.2)
    Methyltransf_15; RNA cap guanine-N2 methyltransferase (PF09445; HMM-score: 16.4)
    NAS; Nicotianamine synthase protein (PF03059; HMM-score: 15.7)
    Methyltransf_8; Hypothetical methyltransferase (PF05148; HMM-score: 15.1)
    Methyltransf_18; Methyltransferase domain (PF12847; HMM-score: 14.2)
    Cons_hypoth95; Conserved hypothetical protein 95 (PF03602; HMM-score: 14)
    no clan defined G6B; G6B family (PF15096; HMM-score: 12.5)
    NADP_Rossmann (CL0063) Spermine_synth; Spermine/spermidine synthase domain (PF01564; HMM-score: 12.2)
    MetW; Methionine biosynthesis protein MetW (PF07021; HMM-score: 12)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.019807
    • TAT(Tat/SPI): 0.001081
    • LIPO(Sec/SPII): 0.001894
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

  • GI:
  • RefSeq: WP_001287240 NCBI
  • UniProt:

⊟Protein sequence[edit | edit source]

  • MSEAGHKFLAKLGKKRLRPGGKRATDWLIAEGGFSKEKRILEVACNRGTTAIKLAQRFCCKITAVDMDAQALEVAKKSAGTAGVAHLISFERANAMKLPYQDASFDIVINEAMLTMQADQAKKKCVMEYLRVLKPGGLLLTHDVLLKEAKESIRQELSQAIHVNVGPLTQDGWEQVMIESGYCDVKVLTGEMTLMKLSGMIYDEGWLGTLKICVNACKKENRKQFLTMYKMFAKNKQKLGFIAMASYKSSKR

⊟Experimental data[edit | edit source]

  • protein localization:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

⊟Expression data[edit | edit source]

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

⊟Relevant publications[edit | edit source]

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