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PangenomeTIGR4
serotype 4
D39
serotype 2
D39V
serotype 2
Hungary19A-6
serotype 19A
EF3030
serotype 19F
670-6B
serotype 6B
6A-10
serotype 6A
70585
serotype 5
A026
serotype 19F
A66
serotype 3
AP200
serotype 11A
ASP0581
serotype 12F
ATCC 49619
serotype 19F
ATCC 700669
serotype 23F
BVJ1JL
serotype 1
CGSP14
serotype 14
G54
serotype 19F
HU-OH
serotype 3
Hu15
serotype 19A
Hu17
serotype 19A
INV104
serotype 1
INV200
serotype 14
JJA
serotype 14
MDRSPN001
serotype 19F
NCTC7466
serotype 2
NU83127
serotype 4
OXC141
serotype 3
P1031
serotype 1
R6
serotype 2
SP49
serotype 19A
SPN032672
serotype 1
SPN034156
serotype 3
SPN034183
serotype 3
SPN994038
serotype 3
SPN994039
serotype 3
SPNA45
serotype 3
ST556
serotype 19F
TCH8431/19A
serotype 19A
Taiwan19F-14
serotype 19F
Xen35
serotype 4
gamPNI0373
serotype 1

NCBI: 13-DEC-2020

⊟Summary[edit | edit source]

  • organism: Streptococcus pneumoniae SPNA45
  • locus tag: SPNA45_RS10855 [old locus tag: SPNA45_01950 ]
  • pan locus tag?: PNEUPAN000739000
  • symbol: SPNA45_RS10855
  • pan gene symbol?: hk08
  • synonym:
  • product: HAMP domain-containing histidine kinase

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SPNA45_RS10855 [old locus tag: SPNA45_01950 ]
  • symbol: SPNA45_RS10855
  • product: HAMP domain-containing histidine kinase
  • replicon: chromosome
  • strand: -
  • coordinates: 1974806..1975858
  • length: 1053
  • essential: unknown

⊟Accession numbers[edit | edit source]

  • Location: NC_018594 (1974806..1975858) NCBI
  • BioCyc: SPNA45_RS10855 BioCyc
  • MicrobesOnline:
  • PneumoBrowse for strain D39V: SPV_0082 PneumoBrowse

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    ATGAAACTAAAAAGTTATATTTTGGTTGGATATATTATTTCAACCCTCTTAACCATTTTG
    GTTGTTTTTTGGGCTGTTCAAAAAATGCTGATTGCGAAAGGCGAGATTTACTTTTTGCTT
    GGGATGACCATCGTTGCCAGCCTTGTCGGTGCTGGGATTAGTCTCTTTCTCCTATTGCCA
    GTCTTTACGTCGTTGGGCAAACTCAAGGAGCATGCCAAGCGGGTAGCGGCCAAGGATTTT
    CCTTCAAATTTGGAGGTTCAAGGTCCTGTAGAATTTCAGCAATTAGGGCAAACTTTTAAT
    GAGATGTCCCATGATTTGCAGGTAAGCTTTGATTCCTTGGAAGAAAGCGAACGAGAAAAG
    GGCTTGATGATTGCCCAGTTGTCGCATGATATTAAGACCCCTATCACTTCGATCCAAGCG
    ACGGTAGAAGGGATTTTGGATGGGATTATCAAGGAGTCGGAGCAAGCTCATTATCTAGCA
    ACCATTGGACGCCAGACGGAGAGGCTCAATAAACTGGTTGAGGAGTTGAATTTTTTGACC
    CTAAACACAGCTAGAAATCAGGTGGAAACTACCAGTAAAGACAGTATTTTTCTGGACAAG
    CTCTTAATTGAGTGCATGAGTGAATTTCAGTTTTTGATTGAGCAGGAGAGAAGAGATGTC
    CACTTGCAGGTAATCCCAGAGTCTGCCCGGATTGAGGGAGATTATGCTAAGCTTTCTCGT
    ATCTTGGTGAATCTGGTCGATAACGCTTTTAAATATTCTGCTCCAGGAACCAAGCTGGAA
    GTGGTGGCTAAGCTGGAGAAGGACCAGCTTTCAATCAGTGTGACCGATGAAGGGCAGGGC
    ATTGCCCCAGAGGATTTGGAAAATATTTTCAAACGCCTTTATCGTGTCGAAACTTCGCGT
    AACATGAAGACAGGTGGTCATGGATTAGGACTTGCGATTGCGCGTGAATTGGCCCATCAA
    TTGGGTGGGGAAATCACAGTCAGCAGCCAGTACGGTCTAGGAAGTACCTTTACCCTTGTT
    CTCAACCTCTCTGGTAGTGAAAATAAAGCCTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1053

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SPNA45_RS10855 [old locus tag: SPNA45_01950 ]
  • symbol: SPNA45_RS10855
  • description: HAMP domain-containing histidine kinase
  • length: 350
  • theoretical pI: 5.29017
  • theoretical MW: 38846.5
  • GRAVY: 0.0325714

⊟Function[edit | edit source]

  • TIGRFAM:
    Signal transduction Signal transduction Two-component systems phosphate regulon sensor kinase PhoR (TIGR02966; EC 2.7.3.-; HMM-score: 170.2)
    heavy metal sensor kinase (TIGR01386; EC 2.7.13.3; HMM-score: 140.9)
    and 12 more
    Signal transduction Signal transduction Two-component systems TMAO reductase sytem sensor TorS (TIGR02956; EC 2.7.13.3; HMM-score: 111.8)
    proteobacterial dedicated sortase system histidine kinase (TIGR03785; HMM-score: 68.4)
    Genetic information processing Protein fate Protein and peptide secretion and trafficking putative PEP-CTERM system histidine kinase (TIGR02916; EC 2.7.13.3; HMM-score: 68.3)
    Signal transduction Signal transduction Two-component systems putative PEP-CTERM system histidine kinase (TIGR02916; EC 2.7.13.3; HMM-score: 68.3)
    Metabolism Central intermediary metabolism Nitrogen fixation nitrogen fixation negative regulator NifL (TIGR02938; HMM-score: 28.6)
    Signal transduction Regulatory functions Protein interactions nitrogen fixation negative regulator NifL (TIGR02938; HMM-score: 28.6)
    anti-sigma B factor (TIGR01924; EC 2.7.11.1; HMM-score: 18.3)
    Genetic information processing DNA metabolism DNA replication, recombination, and repair DNA mismatch repair protein MutL (TIGR00585; HMM-score: 17.6)
    Cellular processes Cellular processes Sporulation and germination anti-sigma F factor (TIGR01925; EC 2.7.11.1; HMM-score: 16)
    Signal transduction Regulatory functions Protein interactions anti-sigma F factor (TIGR01925; EC 2.7.11.1; HMM-score: 16)
    Metabolism Energy metabolism Electron transport cytochrome o ubiquinol oxidase subunit IV (TIGR02847; EC 1.10.3.-; HMM-score: 15.8)
    Genetic information processing DNA metabolism DNA replication, recombination, and repair DNA topoisomerase VI, B subunit (TIGR01052; EC 5.99.1.3; HMM-score: 12.9)
  • TheSEED: data available for D39, Hungary19A-6, TIGR4
  • PFAM:
    His_Kinase_A (CL0025) HATPase_c; Histidine kinase-, DNA gyrase B-, and HSP90-like ATPase (PF02518; HMM-score: 92.6)
    and 7 more
    HisKA; His Kinase A (phospho-acceptor) domain (PF00512; HMM-score: 50.4)
    HAMP (CL0681) HAMP; HAMP domain (PF00672; HMM-score: 37.9)
    His_Kinase_A (CL0025) HATPase_c_3; Histidine kinase-, DNA gyrase B-, and HSP90-like ATPase (PF13589; HMM-score: 34.3)
    HATPase_c_5; GHKL domain (PF14501; HMM-score: 18.7)
    HATPase_c_2; Histidine kinase-like ATPase domain (PF13581; HMM-score: 17.6)
    GT-C (CL0111) YfhO; Bacterial membrane protein YfhO (PF09586; HMM-score: 12.3)
    zf-FYVE-PHD (CL0390) FYVE_2; FYVE-type zinc finger (PF02318; HMM-score: 11.7)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic Membrane
    • Cytoplasmic Score: 0.01
    • Cytoplasmic Membrane Score: 9.99
    • Cellwall Score: 0
    • Extracellular Score: 0
    • Internal Helices: 2
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.008478
    • TAT(Tat/SPI): 0.000176
    • LIPO(Sec/SPII): 0.004839
  • predicted transmembrane helices (TMHMM): 2

⊟Accession numbers[edit | edit source]

  • GI:
  • RefSeq: WP_000769768 NCBI
  • UniProt:

⊟Protein sequence[edit | edit source]

  • MKLKSYILVGYIISTLLTILVVFWAVQKMLIAKGEIYFLLGMTIVASLVGAGISLFLLLPVFTSLGKLKEHAKRVAAKDFPSNLEVQGPVEFQQLGQTFNEMSHDLQVSFDSLEESEREKGLMIAQLSHDIKTPITSIQATVEGILDGIIKESEQAHYLATIGRQTERLNKLVEELNFLTLNTARNQVETTSKDSIFLDKLLIECMSEFQFLIEQERRDVHLQVIPESARIEGDYAKLSRILVNLVDNAFKYSAPGTKLEVVAKLEKDQLSISVTDEGQGIAPEDLENIFKRLYRVETSRNMKTGGHGLGLAIARELAHQLGGEITVSSQYGLGSTFTLVLNLSGSENKA

⊟Experimental data[edit | edit source]

  • protein localization:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator:

⊟Expression data[edit | edit source]

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

⊟Relevant publications[edit | edit source]

Stuart J McKessar, Regine Hakenbeck
The two-component regulatory system TCS08 is involved in cellobiose metabolism of Streptococcus pneumoniae R6.
J Bacteriol: 2007, 189(4);1342-50
[PubMed:17028271] [WorldCat.org] [DOI] (P p)Xin-Ming Song, Wayne Connor, Karsten Hokamp, Lorne A Babiuk, Andrew A Potter
The growth phase-dependent regulation of the pilus locus genes by two-component system TCS08 in Streptococcus pneumoniae.
Microb Pathog: 2009, 46(1);28-35
[PubMed:18983906] [WorldCat.org] [DOI] (P p)Alejandro Gómez-Mejia, Gustavo Gámez, Stephanie Hirschmann, Viktor Kluger, Hermann Rath, Sebastian Böhm, Franziska Voss, Niamatullah Kakar, Lothar Petruschka, Uwe Völker, Reinhold Brückner, Ulrike Mäder, Sven Hammerschmidt
Pneumococcal Metabolic Adaptation and Colonization Are Regulated by the Two-Component Regulatory System 08.
mSphere: 2018, 3(3);
[PubMed:29769380] [WorldCat.org] [DOI] (I e)

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