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PangenomeTIGR4
serotype 4
D39
serotype 2
D39V
serotype 2
Hungary19A-6
serotype 19A
EF3030
serotype 19F
670-6B
serotype 6B
6A-10
serotype 6A
70585
serotype 5
A026
serotype 19F
A66
serotype 3
AP200
serotype 11A
ASP0581
serotype 12F
ATCC 49619
serotype 19F
ATCC 700669
serotype 23F
BM6001
serotype 19F
BVJ1JL
serotype 1
CGSP14
serotype 14
G54
serotype 19F
HU-OH
serotype 3
Hu15
serotype 19A
Hu17
serotype 19A
INV104
serotype 1
INV200
serotype 14
JJA
serotype 14
MDRSPN001
serotype 19F
NCTC7465
serotype 1
NCTC7466
serotype 2
NU83127
serotype 4
OXC141
serotype 3
P1031
serotype 1
R6
serotype 2
SP49
serotype 19A
SPN032672
serotype 1
SPN034156
serotype 3
SPN034183
serotype 3
SPN994038
serotype 3
SPN994039
serotype 3
SPNA45
serotype 3
ST556
serotype 19F
TCH8431/19A
serotype 19A
Taiwan19F-14
serotype 19F
Xen35
serotype 4
gamPNI0373
serotype 1

NCBI: 03-APR-2017

⊟Summary[edit | edit source]

  • organism: Streptococcus pneumoniae Hu15
  • locus tag: SPNHU15_00777 [new locus tag: SPNHU15_RS03640 ]
  • pan locus tag?: PNEUPAN001664000
  • symbol: SPNHU15_00777
  • pan gene symbol?: nanE_1
  • synonym:
  • product: NanE/NanK bifunctional enzyme

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SPNHU15_00777 [new locus tag: SPNHU15_RS03640 ]
  • symbol: SPNHU15_00777
  • product: NanE/NanK bifunctional enzyme
  • replicon: chromosome
  • strand: +
  • coordinates: 739624..740217
  • length: 594
  • essential: unknown

⊟Accession numbers[edit | edit source]

  • Location: CP020551 (739624..740217) NCBI
  • BioCyc:
  • MicrobesOnline:
  • PneumoBrowse for strain D39V:

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    GTGATGCCGTTATTAGCAAAAGCCGCCTTTCAAGCAGGCGCTAAAGGTATTCGTGCTAAT
    TCTGTCCGCGATATTTCAGAAATAAAAGAAGAAGTTGACTTACCAATTATAGGTATCATA
    AAAAGAGATTACGATGGCTTTGAACCTTTTATTTCAGCAACTATGAAAGAGATTGATGAA
    TTAGTTAGTGAAGGAGTAGACATTTTAGCTTTGGATTGTACTAATCGTTCAAGACCAGGT
    TATGATAATATTACTGACTTTATTCATGATATTAAAGTCAAGTATCCAAACAAATTATTA
    ATGGCTGATATTTCTACTTTTGAAGAAGGGAAAGTAGCTGTTGAATCGGGAGTAGATTTT
    GTGGGTACGACATTATCTGGTTATACACCATATAGCCCTAAAAAAGATAATCCTGATTTT
    GAGTTAGTTGAAAGATTGGTAAAAGAGCTTGACGTACCAGTTATTGCTGAAGGAAGAATA
    AGTACTCCGGAACAAGCTAGAAAAATGTTAGATTTAGGTGCTTACGCAGTGGTTGTTGGA
    GGAGCTATCACTCGTCCTTTGGAAATAGCGAAGAAGTTTATTGAGGTAGTTTAG
    60
    120
    180
    240
    300
    360
    420
    480
    540
    594

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SPNHU15_00777 [new locus tag: SPNHU15_RS03640 ]
  • symbol: SPNHU15_00777
  • description: NanE/NanK bifunctional enzyme
  • length: 197
  • theoretical pI: 4.511
  • theoretical MW: 21678.8
  • GRAVY: -0.0314721

⊟Function[edit | edit source]

  • TIGRFAM:
    Unknown function General putative TIM-barrel protein, nifR3 family (TIGR00737; HMM-score: 29.1)
    dihydroorotate dehydrogenase family protein (TIGR01037; EC 1.3.-.-; HMM-score: 27.1)
    Metabolism Amino acid biosynthesis Aromatic amino acid family tryptophan synthase, alpha subunit (TIGR00262; EC 4.2.1.20; HMM-score: 25.6)
    3-hexulose-6-phosphate synthase (TIGR03128; EC 4.1.2.43; HMM-score: 24.1)
    glycosyl amidation-associated protein WbuZ (TIGR03572; HMM-score: 23.8)
    and 13 more
    Metabolism Purines, pyrimidines, nucleosides, and nucleotides Purine ribonucleotide biosynthesis inosine-5'-monophosphate dehydrogenase (TIGR01302; EC 1.1.1.205; HMM-score: 21)
    Unknown function General IMP dehydrogenase family protein (TIGR01304; HMM-score: 20.6)
    mycofactocin system FadH/OYE family oxidoreductase 2 (TIGR03997; EC 1.-.-.-; HMM-score: 19.7)
    Metabolism Amino acid biosynthesis Histidine family 1-(5-phosphoribosyl)-5-[(5-phosphoribosylamino)methylideneamino]imidazole-4-carboxamide isomerase (TIGR00007; EC 5.3.1.16; HMM-score: 19.5)
    Metabolism Amino acid biosynthesis Histidine family imidazoleglycerol phosphate synthase, cyclase subunit (TIGR00735; HMM-score: 19.3)
    Unknown function General putative TIM-barrel protein (TIGR00736; HMM-score: 18)
    geranylgeranylglyceryl phosphate synthase family protein (TIGR01768; EC 2.5.1.-; HMM-score: 17.6)
    Metabolism Energy metabolism Other deoxyribose-phosphate aldolase (TIGR00126; EC 4.1.2.4; HMM-score: 16.1)
    Metabolism Purines, pyrimidines, nucleosides, and nucleotides Other deoxyribose-phosphate aldolase (TIGR00126; EC 4.1.2.4; HMM-score: 16.1)
    Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Pyridoxine pyridoxal 5'-phosphate synthase, synthase subunit Pdx1 (TIGR00343; HMM-score: 16.1)
    Unknown function General hisA/hisF family protein (TIGR00734; HMM-score: 14.9)
    putative enoyl-[acyl-carrier-protein] reductase II (TIGR03151; EC 1.3.1.-; HMM-score: 14.6)
    Metabolism Purines, pyrimidines, nucleosides, and nucleotides Nucleotide and nucleoside interconversions guanosine monophosphate reductase (TIGR01305; EC 1.7.1.7; HMM-score: 11.8)
  • TheSEED: data available for Hungary19A-6
  • PFAM:
    TIM_barrel (CL0036) NanE; Putative N-acetylmannosamine-6-phosphate epimerase (PF04131; HMM-score: 254.4)
    and 14 more
    IMPDH; IMP dehydrogenase / GMP reductase domain (PF00478; HMM-score: 35.6)
    NMO; Nitronate monooxygenase (PF03060; HMM-score: 32.1)
    His_biosynth; Histidine biosynthesis protein (PF00977; HMM-score: 24.6)
    ThiG; Thiazole biosynthesis protein ThiG (PF05690; HMM-score: 23.8)
    Dus; Dihydrouridine synthase (Dus) (PF01207; HMM-score: 23.4)
    Trp_syntA; Tryptophan synthase alpha chain (PF00290; HMM-score: 17.7)
    G3P_antiterm; Glycerol-3-phosphate responsive antiterminator (PF04309; HMM-score: 17.5)
    FMN_dh; FMN-dependent dehydrogenase (PF01070; HMM-score: 17.4)
    TMP-TENI; Thiamine monophosphate synthase (PF02581; HMM-score: 17)
    IGPS; Indole-3-glycerol phosphate synthase (PF00218; HMM-score: 16.2)
    PcrB; PcrB family (PF01884; HMM-score: 15)
    DHO_dh; Dihydroorotate dehydrogenase (PF01180; HMM-score: 12.4)
    Nucleot_cyclase (CL0276) EAL; EAL domain (PF00563; HMM-score: 11.3)
    PLP_aminotran (CL0061) Cys_Met_Meta_PP; Cys/Met metabolism PLP-dependent enzyme (PF01053; HMM-score: 10.4)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • DeepLocPro: Cytoplasmic
    • Cytoplasmic Score: 0.9795
    • Cytoplasmic Membrane Score: 0.0013
    • Cell wall & surface Score: 0.0002
    • Extracellular Score: 0.019
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.016357
    • TAT(Tat/SPI): 0.001481
    • LIPO(Sec/SPII): 0.002941
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

  • GI:
  • RefSeq: ARD36574 NCBI
  • UniProt:

⊟Protein sequence[edit | edit source]

  • MMPLLAKAAFQAGAKGIRANSVRDISEIKEEVDLPIIGIIKRDYDGFEPFISATMKEIDELVSEGVDILALDCTNRSRPGYDNITDFIHDIKVKYPNKLLMADISTFEEGKVAVESGVDFVGTTLSGYTPYSPKKDNPDFELVERLVKELDVPVIAEGRISTPEQARKMLDLGAYAVVVGGAITRPLEIAKKFIEVV

⊟Experimental data[edit | edit source]

  • protein localization:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator:

⊟Transcription[edit | edit source]

  • transcription start site:

⊟Expression data[edit | edit source]

  • PneumoExpress for strain D39V:

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You can add further information about the gene and protein here. [edit]

⊟Literature[edit | edit source]

⊟References[edit | edit source]

⊟Relevant publications[edit | edit source]

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