PangenomeTIGR4

serotype 4

D39

serotype 2

D39V

serotype 2

Hungary19A-6

serotype 19A

EF3030

serotype 19F

670-6B

serotype 6B

6A-10

serotype 6A

70585

serotype 5

A026

serotype 19F

A66

serotype 3

AP200

serotype 11A

ASP0581

serotype 12F

ATCC 49619

serotype 19F

ATCC 700669

serotype 23F

BVJ1JL

serotype 1

CGSP14

serotype 14

G54

serotype 19F

HU-OH

serotype 3

Hu15

serotype 19A

Hu17

serotype 19A

INV104

serotype 1

INV200

serotype 14

JJA

serotype 14

MDRSPN001

serotype 19F

NCTC7466

serotype 2

NU83127

serotype 4

OXC141

serotype 3

P1031

serotype 1

R6

serotype 2

SP49

serotype 19A

SPN032672

serotype 1

SPN034156

serotype 3

SPN034183

serotype 3

SPN994038

serotype 3

SPN994039

serotype 3

SPNA45

serotype 3

ST556

serotype 19F

TCH8431/19A

serotype 19A

Taiwan19F-14

serotype 19F

Xen35

serotype 4

gamPNI0373

serotype 1

NCBI: 03-APR-2017

⊟Summary

• organism: Streptococcus pneumoniae Hu15
• locus tag: SPNHU15_02204 [new locus tag: SPNHU15_RS10430 ]
• pan locus tag^?: PNEUPAN003873000
• symbol: SPNHU15_02204
• pan gene symbol^?: fucR
• synonym:
• product: regulator of fucose operon

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: SPNHU15_02204 [new locus tag: SPNHU15_RS10430 ]
• symbol: SPNHU15_02204
• product: regulator of fucose operon
• replicon: chromosome
• strand: +
• coordinates: 2073884..2074657
• length: 774
• essential: unknown

⊟Accession numbers

• Location: CP020551 (2073884..2074657) NCBI
• BioCyc:
• MicrobesOnline:
• PneumoBrowse for strain D39V: SPV_1996 PneumoBrowse

⊟Phenotype

Share your knowledge and add information here. [edit]

⊟DNA sequence

• 1
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  ATGATAAAAGATGAACGTGTACTTGAATTGATTGAAATTATTAAAAAGAAAAAAAGAATT
  GCCGTAAAAGAGCTGGCAGAAATCACTTTCTCCAGCACAAGTACCTTACGTCGTGATTTA
  ATTTTCTTAGAAAATCAAGGTCTTATCAAAAGAAAGCACGGATACGTGACCCTGTCCTCT
  ATGAACACAATTGAACTTTCTCATCAAATACGGGAAGGAGAAAGTACTAGGCAAAAAAGA
  CTAATCGCTAGTCTCGCTAAAGACTTTATTCGGTCAGGTATGTGTGTTTATCTGGATTCC
  AGTACGACTGTCTACGAACTTTGTCCCTATCTTTCTGAACTTGATAATTTGATTATTTTT
  ACAAATGGTTTACATACTGCACAAACCCTATCTGAAACTGTTAAAGATAGCTCCAAAATC
  TTTATCACATCTGGCGAGGTCAAACATCAATCCTGTTCCGTGGTCAATTACGATAAGGAA
  AATTCTTTATTAGATCATTTTAATATCGATTTAGCATTTTGTTCAGCAAGAGGTATTGAT
  GACCAATATGTTTATGAAGCTTCTCTCAGCCAAGCTATTTCAAAAAAGAATATTATTGAC
  AAAGCCCATGAAACCATCTTACTGATTGATAGCTCTAAATTTTACAAGACTGGATTTTTT
  AAAATTAATCCCCTCTCCAAATACACAACCTTTATTTCTGACACCTTGCCAAATCAAAAA
  TTATTAGACGCAGTCGAATTATTTGATGGAGAATGGGTTTCTGATATTCAATGA
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  774
  

⊟Protein

⊟General

• locus tag: SPNHU15_02204 [new locus tag: SPNHU15_RS10430 ]
• symbol: SPNHU15_02204
• description: regulator of fucose operon
• length: 257
• theoretical pI: 6.51676
• theoretical MW: 29282.3
• GRAVY: -0.187938

⊟Function

• TIGRFAM:
  Signal transduction Regulatory functions DNA interactions CRISPR locus-related DNA-binding protein (TIGR01884; HMM-score: 20.6)
  and 1 more
  Signal transduction Regulatory functions DNA interactions beta-ketoadipate pathway transcriptional regulators, PcaR/PcaU/PobR family (TIGR02431; HMM-score: 13.3)
• TheSEED: data available for D39, Hungary19A-6, TIGR4
• PFAM:
  ISOCOT_Fold (CL0246) DeoRC; DeoR C terminal sensor domain (PF00455; HMM-score: 150.5)
  and 11 more
  HTH (CL0123) HTH_DeoR; DeoR-like helix-turn-helix domain (PF08220; HMM-score: 44.3)
  HTH_24; Winged helix-turn-helix DNA-binding (PF13412; HMM-score: 19.3)
  HTH_Mga; M protein trans-acting positive regulator (MGA) HTH domain (PF08280; HMM-score: 18.6)
  HTH_11; HTH domain (PF08279; HMM-score: 17.6)
  HTH_5; Bacterial regulatory protein, arsR family (PF01022; HMM-score: 15.8)
  GntR; Bacterial regulatory proteins, gntR family (PF00392; HMM-score: 14.9)
  HTH_IclR; IclR helix-turn-helix domain (PF09339; HMM-score: 14.5)
  MarR; MarR family (PF01047; HMM-score: 12.9)
  LexA_DNA_bind; LexA DNA binding domain (PF01726; HMM-score: 12.5)
  HTH_Crp_2; Crp-like helix-turn-helix domain (PF13545; HMM-score: 12.2)
  HTH_PafC; PafC helix-turn-helix domain (PF19187; HMM-score: 12.1)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:
• genes regulated by FucR*, TF important in Fucose utilization: in TIGR4

⊟Localization

• PSORTb: Cytoplasmic
  • Cytoplasmic Score: 7.5
  • Cytoplasmic Membrane Score: 1.15
  • Cellwall Score: 0.62
  • Extracellular Score: 0.73
  • Internal Helices: 0
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.003261
  • TAT(Tat/SPI): 0.000512
  • LIPO(Sec/SPII): 0.000367
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI:
• RefSeq: ARD37947 NCBI
• UniProt:

⊟Protein sequence

• MIKDERVLELIEIIKKKKRIAVKELAEITFSSTSTLRRDLIFLENQGLIKRKHGYVTLSSMNTIELSHQIREGESTRQKRLIASLAKDFIRSGMCVYLDSSTTVYELCPYLSELDNLIIFTNGLHTAQTLSETVKDSSKIFITSGEVKHQSCSVVNYDKENSLLDHFNIDLAFCSARGIDDQYVYEASLSQAISKKNIIDKAHETILLIDSSKFYKTGFFKINPLSKYTTFISDTLPNQKLLDAVELFDGEWVSDIQ

⊟Experimental data

• protein localization:
• interaction partners:

⊟Expression & Regulation

⊟Operon

⊟Regulation

• data available for TIGR4

⊟Expression data

• PneumoExpress for strain D39V: SPV_1996 PneumoExpress

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications

Pan F Chan, Karen M O'Dwyer, Leslie M Palmer, Jennifer D Ambrad, Karen A Ingraham, Chi So, Michael A Lonetto, Sanjoy Biswas, Martin Rosenberg, David J Holmes, Magdalena Zalacain
Characterization of a novel fucose-regulated promoter (PfcsK) suitable for gene essentiality and antibacterial mode-of-action studies in Streptococcus pneumoniae.
J Bacteriol: 2003, 185(6);2051-8
[PubMed:12618474] [WorldCat.org] [DOI] (P p)Irfan Manzoor, Sulman Shafeeq, Muhammad Afzal, Oscar P Kuipers
Fucose-Mediated Transcriptional Activation of the fcs Operon by FcsR in Streptococcus pneumoniae.
J Mol Microbiol Biotechnol: 2015, 25(2-3);120-8
[PubMed:26159073] [WorldCat.org] [DOI] (I p)