PangenomeTIGR4

serotype 4

D39

serotype 2

D39V

serotype 2

Hungary19A-6

serotype 19A

EF3030

serotype 19F

670-6B

serotype 6B

6A-10

serotype 6A

70585

serotype 5

A026

serotype 19F

A66

serotype 3

AP200

serotype 11A

ASP0581

serotype 12F

ATCC 49619

serotype 19F

ATCC 700669

serotype 23F

BM6001

serotype 19F

BVJ1JL

serotype 1

CGSP14

serotype 14

G54

serotype 19F

HU-OH

serotype 3

Hu15

serotype 19A

Hu17

serotype 19A

INV104

serotype 1

INV200

serotype 14

JJA

serotype 14

MDRSPN001

serotype 19F

NCTC7465

serotype 1

NCTC7466

serotype 2

NU83127

serotype 4

OXC141

serotype 3

P1031

serotype 1

R6

serotype 2

SP49

serotype 19A

SPN032672

serotype 1

SPN034156

serotype 3

SPN034183

serotype 3

SPN994038

serotype 3

SPN994039

serotype 3

SPNA45

serotype 3

ST556

serotype 19F

TCH8431/19A

serotype 19A

Taiwan19F-14

serotype 19F

Xen35

serotype 4

gamPNI0373

serotype 1

NCBI: 07-MAR-2025

⊟Summary

• organism: Streptococcus pneumoniae Hu15
• locus tag: SPNHU15_RS00475 [old locus tag: SPNHU15_00093 ]
• pan locus tag^?: PNEUPAN003043000
• symbol: SPNHU15_RS00475
• pan gene symbol^?: —
• synonym:
• product: DUF2974 domain-containing protein

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: SPNHU15_RS00475 [old locus tag: SPNHU15_00093 ]
• symbol: SPNHU15_RS00475
• product: DUF2974 domain-containing protein
• replicon: chromosome
• strand: +
• coordinates: 84718..85791
• length: 1074
• essential: unknown

⊟Accession numbers

• Location: NZ_CP020551 (84718..85791) NCBI
• BioCyc:
• MicrobesOnline:
• PneumoBrowse for strain D39V: SPV_1447 PneumoBrowse

⊟Phenotype

Share your knowledge and add information here. [edit]

⊟DNA sequence

• 1
  61
  121
  181
  241
  301
  361
  421
  481
  541
  601
  661
  721
  781
  841
  901
  961
  1021
  
  ATGGCCAATATTTTTGACTATCTGAAAGATGTCGCATATGATTCTTATTACGACCTTCCC
  TTGAATGAGTTAGACATTCTAACCTTAATAGAAATCACCTACCTCTCCTTTGATAATCTG
  GTCTCCACACTTCCTCAACGTCTTTTAGATCTAGCACCTCAGGTTCCAAGAGATCCCACC
  ATGCTTACTAGCAAAAATCGCCTTCAATTATTAGATGAATTGGCTCAACACAAGCGCTTC
  AAAAATTGCAAACTCTCCCATTTTATCAACGACATCGACCCTGAACTGCAAAAGCAATTT
  GCGGCTATGACTTATCGTGTCAGCCTCGATACCTATCTGATTGTCTTTCGTGGGACAGAT
  GACAGTATCATTGGCTGGAAGGAAGATTTCCACCTGACCTATATGAAGGAAATTCCTGCT
  CAAAAGCACGCCCTTCGCTATTTAAAGAACTTTTTTGCCCATCATCCTAAGCAAAAGGTT
  ATTCTAGCTGGGCATTCCAAGGGAGGAAATCTCGCTATCTATGCTGCTAGCCAAATTGAG
  CAAAGTTTGCAAAATCAGATCACAGCAGTTTATACATTTGATGCACCTGGTCTCCATCAA
  GAATTGACACAGACTGCGGGTTATCAAAGGATAATGGATAGAAGCAAGATATTCATTCCA
  CAAGGTTCCATTATCGGTATGATGCTGGAAATTCCTGCTCACCAAATCATCGTTCAGAGT
  ACTGCCCTGGGTGGCATCGCCCAGCACGATACCTTTAGTTGGCAGATTGAGGACAAGCAC
  TTCGTCCAACTGGATAAGACCAACAGTGATAGCCAGCAAGTAGACACAACCTTTAAAGAA
  TGGGTGGCCACAGTCCCTGACGAAGAACTTCAGCTCTACTTCGACCTCTTCTTTGGCACT
  ATTCTTGAGGCTGGTATTAGCTCTATCAATGACTTGGCTTCCTTAAAGGCGCTTGAATAC
  ATTCATCATCTCTTTGTCCAAGCTCAATCCCTCACTCCAGAAGAAAGAGAAACCTTGGGT
  CGCCTTACCCAGTTATTGATTGATACTCGTTACCAGGCATGGAAAAATAGATAA
  60
  120
  180
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  360
  420
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  540
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  720
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  840
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  960
  1020
  1074
  

⊟Protein

⊟General

• locus tag: SPNHU15_RS00475 [old locus tag: SPNHU15_00093 ]
• symbol: SPNHU15_RS00475
• description: DUF2974 domain-containing protein
• length: 357
• theoretical pI: 5.71318
• theoretical MW: 41071.5
• GRAVY: -0.233053

⊟Function

• reaction:
  EC 3.-.-.-?  ExPASy
• TIGRFAM:
  Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Menaquinone and ubiquinone 2-succinyl-6-hydroxy-2,4-cyclohexadiene-1-carboxylate synthase (TIGR03695; EC 4.2.99.20; HMM-score: 15.3)
• TheSEED: data available for D39, Hungary19A-6, TIGR4
• PFAM:
  AB_hydrolase (CL0028) Mbeg1-like; Mbeg1-like (PF11187; HMM-score: 297.5)
  and 13 more
  Lipase_3; Lipase (class 3) (PF01764; HMM-score: 30.9)
  Abhydrolase_3; alpha/beta hydrolase fold (PF07859; HMM-score: 19.8)
  Cutinase; Cutinase (PF01083; HMM-score: 19.7)
  Thioesterase; Thioesterase domain (PF00975; HMM-score: 19.2)
  PGAP1; PGAP1-like protein (PF07819; HMM-score: 18.7)
  Abhydrolase_1; alpha/beta hydrolase fold (PF00561; HMM-score: 17.3)
  Hydrolase_4; Serine aminopeptidase, S33 (PF12146; HMM-score: 17)
  BD-FAE; BD-FAE (PF20434; HMM-score: 14.9)
  DUF3089; Protein of unknown function (DUF3089) (PF11288; HMM-score: 13.8)
  DUF900; Alpha/beta hydrolase of unknown function (DUF900) (PF05990; HMM-score: 12.8)
  Abhydrolase_11; Alpha/beta hydrolase domain (PF20408; HMM-score: 12.2)
  PAE; Pectinacetylesterase (PF03283; HMM-score: 12.1)
  DUF1749; Protein of unknown function (DUF1749) (PF08538; HMM-score: 12.1)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: Cytoplasmic
  • Cytoplasmic Score: 7.5
  • Cytoplasmic Membrane Score: 1.15
  • Cellwall Score: 0.62
  • Extracellular Score: 0.73
  • Internal Helices: 0
• DeepLocPro: Cytoplasmic
  • Cytoplasmic Score: 0.7155
  • Cytoplasmic Membrane Score: 0.2726
  • Cell wall & surface Score: 0.0018
  • Extracellular Score: 0.0101
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.001687
  • TAT(Tat/SPI): 0.000084
  • LIPO(Sec/SPII): 0.000173
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI:
• RefSeq: WP_001273814 NCBI
• UniProt:

⊟Protein sequence

• MANIFDYLKDVAYDSYYDLPLNELDILTLIEITYLSFDNLVSTLPQRLLDLAPQVPRDPTMLTSKNRLQLLDELAQHKRFKNCKLSHFINDIDPELQKQFAAMTYRVSLDTYLIVFRGTDDSIIGWKEDFHLTYMKEIPAQKHALRYLKNFFAHHPKQKVILAGHSKGGNLAIYAASQIEQSLQNQITAVYTFDAPGLHQELTQTAGYQRIMDRSKIFIPQGSIIGMMLEIPAHQIIVQSTALGGIAQHDTFSWQIEDKHFVQLDKTNSDSQQVDTTFKEWVATVPDEELQLYFDLFFGTILEAGISSINDLASLKALEYIHHLFVQAQSLTPEERETLGRLTQLLIDTRYQAWKNR

⊟Experimental data

• protein localization:
• interaction partners:

⊟Expression & Regulation

⊟Operon

⊟Regulation

• regulator:

⊟Transcription

• transcription start site:

⊟Expression data

• PneumoExpress for strain D39V: SPV_1447 PneumoExpress

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You can add further information about the gene and protein here. [edit]

⊟Literature

⊟References

⊟Relevant publications