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PangenomeTIGR4
serotype 4
D39
serotype 2
D39V
serotype 2
Hungary19A-6
serotype 19A
EF3030
serotype 19F
670-6B
serotype 6B
6A-10
serotype 6A
70585
serotype 5
A026
serotype 19F
A66
serotype 3
AP200
serotype 11A
ASP0581
serotype 12F
ATCC 49619
serotype 19F
ATCC 700669
serotype 23F
BVJ1JL
serotype 1
CGSP14
serotype 14
G54
serotype 19F
HU-OH
serotype 3
Hu15
serotype 19A
Hu17
serotype 19A
INV104
serotype 1
INV200
serotype 14
JJA
serotype 14
MDRSPN001
serotype 19F
NCTC7466
serotype 2
NU83127
serotype 4
OXC141
serotype 3
P1031
serotype 1
R6
serotype 2
SP49
serotype 19A
SPN032672
serotype 1
SPN034156
serotype 3
SPN034183
serotype 3
SPN994038
serotype 3
SPN994039
serotype 3
SPNA45
serotype 3
ST556
serotype 19F
TCH8431/19A
serotype 19A
Taiwan19F-14
serotype 19F
Xen35
serotype 4
gamPNI0373
serotype 1

NCBI: 03-APR-2017

⊟Summary[edit | edit source]

  • organism: Streptococcus pneumoniae Hu17
  • locus tag: SPNHU17_00063 [new locus tag: SPNHU17_RS00320 ]
  • pan locus tag?: PNEUPAN000440000
  • symbol: SPNHU17_00063
  • pan gene symbol?: lytA_1
  • synonym: lytA
  • product: lytic amidase (N-acetylmuramoyl-L-alanine amidase)

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SPNHU17_00063 [new locus tag: SPNHU17_RS00320 ]
  • symbol: SPNHU17_00063
  • product: lytic amidase (N-acetylmuramoyl-L-alanine amidase)
  • replicon: chromosome
  • strand: +
  • coordinates: 53666..54634
  • length: 969
  • essential: unknown

⊟Accession numbers[edit | edit source]

  • Location: CP020549 (53666..54634) NCBI
  • BioCyc: see SPNHU17_RS00320
  • MicrobesOnline:
  • PneumoBrowse for strain D39V:

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    ATGGATATCGATAGAAACAGACTACGTACAGGCTTGCCCCAGGTTGGGGTGCAGCCTTAT
    CGACAAGTACATGCTCACTCAACAGGTAACCGCAACTCAACCGTACAGAATGAAGCGGAT
    TATCACTATAGAAAGGACCCTGAACTAGGGTTCTTTTCACATGTTGTCGGAAACGGCCGT
    GTCATGCAAGTAGGTCCTGTGAACAACGGAAGTTGGGATGTTGGGGGCGGTTGGAATACT
    GAGAGTTACGCAGCGGTTGAACTGATTGAAAGCCATTCAACTAAAGAAGAGTTCATGACG
    GACTACCGCCTCTATATCGAATTGCTACGCAATCTAGCGGACGAAGCAGGCTTGCCGAAG
    ACTCTTGATACAGACGACTTGGCAGGTATCAAGACGCATGAATACTGTACCAATAACCAA
    CCAAACAACCACTCAGACCATGTGGATCCATATCCATATCTTGCTAAATGGGGCATTAGC
    CGTGAACAGTTTAAGCAAGACATCGAAAACGGCTTGAGCGCTGCAACAGGCTGGCAGAAA
    AATGGCACTGGCTACTGGTACGTACACTCAGACGGCTCTTATCCAAAAGATAAGTTTGAG
    AAAATCAACGGTACCTGGTATTATTTCGATGGCTCAGGCTATATGCTTTCAGACCGCTGG
    AAGAAGCACACAGACGGCAACTGGTACTACTTTGACCAATCAGGCGAAATGGCTACAGGC
    TGGAAGAAAATCGCTGAGAAGTGGTACTATTTTGATGTAGAAGGTGCCATGAAGACAGGC
    TGGGTCAAGTACAAGGACACTTGGTACTACTTAGACGCTAAAGAAGGCGCCAAAGAAGGC
    GCCATGGTATCAAATGCCTTTATCCAGTCAGCGGACGGAACAGGCTGGTACTACCTCAAA
    CCAGACGGAACACTGGCAGATAAACCAGAGTTCACAGTAGAGCCAGATGGCTTGATTACA
    GTAAAATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    969

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SPNHU17_00063 [new locus tag: SPNHU17_RS00320 ]
  • symbol: SPNHU17_00063
  • description: lytic amidase (N-acetylmuramoyl-L-alanine amidase)
  • length: 322
  • theoretical pI: 5.2381
  • theoretical MW: 36878.4
  • GRAVY: -0.839441

⊟Function[edit | edit source]

  • TIGRFAM:
    glucan-binding repeat (TIGR04035; HMM-score: 51.2)
  • TheSEED: data available for Hungary19A-6
  • PFAM:
    Choline_binding (CL0694) Choline_bind_1; Putative cell wall binding repeat (PF01473; HMM-score: 94.1)
    Choline_bind_3; Choline-binding repeat (PF19127; HMM-score: 90.6)
    and 3 more
    no clan defined Amidase_2; N-acetylmuramoyl-L-alanine amidase (PF01510; HMM-score: 27)
    Choline_binding (CL0694) Choline_bind_2; Choline-binding repeat (PF19085; HMM-score: 20.5)
    TRASH (CL0175) YHS; YHS domain (PF04945; HMM-score: 6.8)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: unknown (no significant prediction)
    • Cytoplasmic Score: 2.5
    • Cytoplasmic Membrane Score: 2.5
    • Cellwall Score: 2.5
    • Extracellular Score: 2.5
    • Internal Helices: 0
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.055761
    • TAT(Tat/SPI): 0.024019
    • LIPO(Sec/SPII): 0.008741
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

  • GI:
  • RefSeq: ARD33692 NCBI
  • UniProt:

⊟Protein sequence[edit | edit source]

  • MDIDRNRLRTGLPQVGVQPYRQVHAHSTGNRNSTVQNEADYHYRKDPELGFFSHVVGNGRVMQVGPVNNGSWDVGGGWNTESYAAVELIESHSTKEEFMTDYRLYIELLRNLADEAGLPKTLDTDDLAGIKTHEYCTNNQPNNHSDHVDPYPYLAKWGISREQFKQDIENGLSAATGWQKNGTGYWYVHSDGSYPKDKFEKINGTWYYFDGSGYMLSDRWKKHTDGNWYYFDQSGEMATGWKKIAEKWYYFDVEGAMKTGWVKYKDTWYYLDAKEGAKEGAMVSNAFIQSADGTGWYYLKPDGTLADKPEFTVEPDGLITVK

⊟Experimental data[edit | edit source]

  • protein localization:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator:

⊟Expression data[edit | edit source]

  • PneumoExpress for strain D39V:

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

⊟Relevant publications[edit | edit source]

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