⊟Summary[edit | edit source]
- organism: Streptococcus pneumoniae P1031
- locus tag: SPP_RS11050 [old locus tag: SPP_2242 ]
- pan locus tag?: PNEUPAN003841000
- symbol: pspC
- pan gene symbol?: pspC
- synonyms: cbpA, pbcA, spsA
- product: pneumococcal surface protein PspC, choline-binding form
⊟Genome View[edit | edit source]
⊟Gene[edit | edit source]
⊟General[edit | edit source]
- type: CDS
- locus tag: SPP_RS11050 [old locus tag: SPP_2242 ]
- symbol: pspC
- product: pneumococcal surface protein PspC, choline-binding form
- replicon: chromosome
- strand: -
- coordinates: 2061360..2063099
- length: 1740
- essential: unknown
⊟Accession numbers[edit | edit source]
- Location: NC_012467 (2061360..2063099) NCBI
- BioCyc: SPP_RS11050 BioCyc
- MicrobesOnline: see SPP_2242
- PneumoBrowse for strain D39V: SPV_2017 PneumoBrowse
⊟Phenotype[edit | edit source]
Share your knowledge and add information here. [edit]
⊟DNA sequence[edit | edit source]
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1681ATGTTTGCATCAAAAAGCGAAAGAAAAGTACATTATTCAATTCGTAAATTTAGTATTGGA
GTAGCTAGTGTAGTAGTTGCCAGTCTTGTTATGGGGAGTGTGGTTCATGCGACAGAGAAC
GAGGGAAGTACCCAAGCAGCCACTTTTTCTAATATGGCAAATAAAAGTCAGACAGAACAA
GGAGAAATCAATATAGAACGAGATAAGGCAAAGACAGCGGTCAGTGAATATAAAGAAAAA
AAAGTGAGTGAGATCTATACAAAATTAGAGAGAGATAGACATAAAGATACTGTAGATCTA
GTTAACAAGTTGCAAGAGATTAAGAACGAGTATTTGAATAAAATAGTTCAATCAACCTCA
AAAACCGAAATACAGGGACTTATTACAACAAGTCGATCAAAACTAGATGAAGCTGTGTCT
AAATATAAAAAGGCCCCATCTTCTTCGTCAAGTTCAGGCTCTTCCACTAAACCGGAAGCT
TCAGATACAGCGAAGCCAAACAAGCCGACAGAACTAGAAAAAAAGGTAGCAGAAGCTGAG
AAGAAGGTTGAAGAAGCTAAGAAGAAAGCCAAGGATCAAAAAGAAGAAGATTACCGTAAC
TACCCAACCATTACTTACAAAACGCTTGAACTTGAAATTGCTGAGTCCGATGTGGAAGTT
AAAAAAGCGGAGCTTGAACTAGTAAAAGAGGAAGCTAAGGAACCTCGAAACGAGGAAAAA
GTTAAGCAAGCAAAAGCGAAAGTTGAGAGTGAAGAAACTGAGGCTACAAGGTTAGAAAAA
ATCAAGACAGATCGTAAAAAAGCAGAAGAAGAAGCTAAACGAAAAGCAGCAGAAGAAGAT
AAAGTTAAAGAAAAACCAGCTGAACAACCAAAACCAGCGCCGGCTCCTCAACCAGAAAAA
CCAGCTCCAAAACCAGAGAATCCAGCTCCAAAACCAGAGAATCCAGCTCCAAAACCAGAA
AAACCAGCTGAACAACAAGCTGAAGAAGACTATGCTCGTAGATCAGAAGAAGAATATAAT
CGCTTGACTCAACAGCAACCGCCAAAAACTGAAAAACCAGCACAACCATCTACTCCAAAA
ACAGGCTGGAAACAAGAAAACGGTATGTGGTACTTCTACAATACTGATGGTTCAATGGCG
ACAGGATGGCTCCAAAACAATGGCTCATGGTACTACCTCAACAGCAATGGCGCTATGGCG
ACAGGATGGCTCCAAAACAATGGTTCATGGTACTATCTAAACGCTAATGGTTCAATGGCA
ACAGGATGGCTCCAAAACAATGGTTCATGGTACTACCTAAACGCTAATGGTTCAATGGCG
ACAGGATGGCTCCAATACAATGGTTCATGGTACTACCTAAACGCTAATGGTGATATGGCG
ACAGGATGGCTCCAATACAATGGCTCATGGTACTACCTAAACGCTAATGGTGATATGGCG
ACAGGATGGCTCCAATACAATGGCTCATGGTACTACCTAAACGCTAATGGTGATATGGCG
ACAGGATGGCTCCAATACAATGGCTCATGGTACTACCTAAACGCTAATGGTGATATGGCG
ACAGGTTGGGTGAAAGATGGAGATACCTGGTACTATCTTGAAGCATCAGGTGCTATGAAA
GCAAGCCAATGGTTCAAAGTATCAGATAAATGGTACTATGTCAATGGCTCAGGTGCCCTT
GCAGTCAACACAACTGTAGATGGCTATGGAGTCAATGCCAATGGTGAATGGGTAAACTAA60
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⊟Protein[edit | edit source]
⊟General[edit | edit source]
- locus tag: SPP_RS11050 [old locus tag: SPP_2242 ]
- symbol: PspC
- description: pneumococcal surface protein PspC, choline-binding form
- length: 579
- theoretical pI: 5.78549
- theoretical MW: 65239.7
- GRAVY: -1.0266
⊟Function[edit | edit source]
- TIGRFAM: glucan-binding repeat (TIGR04035; HMM-score: 60.9)gram-positive signal peptide, YSIRK family (TIGR01168; HMM-score: 53.3)
- TheSEED: see SPP_2242
- PFAM: Choline_binding (CL0694) Choline_bind_3; Choline-binding repeat (PF19127; HMM-score: 199.1)Choline_bind_1; Putative cell wall binding repeat (PF01473; HMM-score: 197.8)and 5 moreno clan defined RICH; RICH domain (PF05062; HMM-score: 85.8)Choline_binding (CL0694) Choline_bind_2; Choline-binding repeat (PF19085; HMM-score: 44.4)no clan defined YSIRK_signal; YSIRK type signal peptide (PF04650; HMM-score: 41.2)CsgA; Minor curli fiber component A (PF17334; HMM-score: 18)DUF4224; Domain of unknown function (DUF4224) (PF13986; HMM-score: 14)
⊟Structure, modifications & cofactors[edit | edit source]
- domains:
- modifications:
- cofactors:
- effectors:
⊟Localization[edit | edit source]
- PSORTb: Extracellular
- Cytoplasmic Score: 0.01
- Cytoplasmic Membrane Score: 0.09
- Cellwall Score: 0.18
- Extracellular Score: 9.72
- Internal Helix: 1
- SignalP: Signal peptide SP(Sec/SPI) length 37 aa
- SP(Sec/SPI): 0.980827
- TAT(Tat/SPI): 0.011453
- LIPO(Sec/SPII): 0.002242
- Cleavage Site: CS pos: 37-38. VHA-TE. Pr: 0.9137
- predicted transmembrane helices (TMHMM): 0
⊟Accession numbers[edit | edit source]
- GI:
- RefSeq: WP_000458176 NCBI
- UniProt:
⊟Protein sequence[edit | edit source]
- MFASKSERKVHYSIRKFSIGVASVVVASLVMGSVVHATENEGSTQAATFSNMANKSQTEQGEINIERDKAKTAVSEYKEKKVSEIYTKLERDRHKDTVDLVNKLQEIKNEYLNKIVQSTSKTEIQGLITTSRSKLDEAVSKYKKAPSSSSSSGSSTKPEASDTAKPNKPTELEKKVAEAEKKVEEAKKKAKDQKEEDYRNYPTITYKTLELEIAESDVEVKKAELELVKEEAKEPRNEEKVKQAKAKVESEETEATRLEKIKTDRKKAEEEAKRKAAEEDKVKEKPAEQPKPAPAPQPEKPAPKPENPAPKPENPAPKPEKPAEQQAEEDYARRSEEEYNRLTQQQPPKTEKPAQPSTPKTGWKQENGMWYFYNTDGSMATGWLQNNGSWYYLNSNGAMATGWLQNNGSWYYLNANGSMATGWLQNNGSWYYLNANGSMATGWLQYNGSWYYLNANGDMATGWLQYNGSWYYLNANGDMATGWLQYNGSWYYLNANGDMATGWLQYNGSWYYLNANGDMATGWVKDGDTWYYLEASGAMKASQWFKVSDKWYYVNGSGALAVNTTVDGYGVNANGEWVN
⊟Experimental data[edit | edit source]
- protein localization:
- interaction partners:
⊟Expression & Regulation[edit | edit source]
⊟Operon[edit | edit source]
- MicrobesOnline: no polycistronic organisation predicted
⊟Regulation[edit | edit source]
- regulator:
⊟Expression data[edit | edit source]
- PneumoExpress for strain D39V: SPV_2017 PneumoExpress
⊟Biological Material[edit | edit source]
⊟Mutants[edit | edit source]
⊟Expression vector[edit | edit source]
⊟lacZ fusion[edit | edit source]
⊟GFP fusion[edit | edit source]
⊟two-hybrid system[edit | edit source]
⊟FLAG-tag construct[edit | edit source]
⊟Antibody[edit | edit source]
⊟Other Information[edit | edit source]
You are kindly invited to share additional interesting facts.
⊟Literature[edit | edit source]
⊟References[edit | edit source]
⊟Relevant publications[edit | edit source]
S Hammerschmidt, G Bethe, P H Remane, G S Chhatwal
Identification of pneumococcal surface protein A as a lactoferrin-binding protein of Streptococcus pneumoniae.
Infect Immun: 1999, 67(4);1683-7
[PubMed:10085004] [WorldCat.org] [DOI] (P p)S Hammerschmidt, M P Tillig, S Wolff, J P Vaerman, G S Chhatwal
Species-specific binding of human secretory component to SpsA protein of Streptococcus pneumoniae via a hexapeptide motif.
Mol Microbiol: 2000, 36(3);726-36
[PubMed:10844660] [WorldCat.org] [DOI] (P p)Hanna Jarva, Robert Janulczyk, Jens Hellwage, Peter F Zipfel, Lars Björck, Seppo Meri
Streptococcus pneumoniae evades complement attack and opsonophagocytosis by expressing the pspC locus-encoded Hic protein that binds to short consensus repeats 8-11 of factor H.
J Immunol: 2002, 168(4);1886-94
[PubMed:11823523] [WorldCat.org] [DOI] (P p)Francesco Iannelli, Marco R Oggioni, Gianni Pozzi
Allelic variation in the highly polymorphic locus pspC of Streptococcus pneumoniae.
Gene: 2002, 284(1-2);63-71
[PubMed:11891047] [WorldCat.org] [DOI] (P p)Sandhya Dave, Stephanie Carmicle, Sven Hammerschmidt, Michael K Pangburn, Larry S McDaniel
Dual roles of PspC, a surface protein of Streptococcus pneumoniae, in binding human secretory IgA and factor H.
J Immunol: 2004, 173(1);471-7
[PubMed:15210807] [WorldCat.org] [DOI] (P p)Christine Elm, Manfred Rohde, Jean-Pierre Vaerman, Gursharan S Chhatwal, Sven Hammerschmidt
Characterization of the interaction of the pneumococcal surface protein SpsA with the human polymeric immunoglobulin receptor (hpIgR).
Indian J Med Res: 2004, 119 Suppl;61-5
[PubMed:15232164] [WorldCat.org] (P p)Sven Hammerschmidt, Vaibhav Agarwal, Anja Kunert, Steffi Haelbich, Christine Skerka, Peter F Zipfel
The host immune regulator factor H interacts via two contact sites with the PspC protein of Streptococcus pneumoniae and mediates adhesion to host epithelial cells.
J Immunol: 2007, 178(9);5848-58
[PubMed:17442969] [WorldCat.org] [DOI] (P p)Antoine Dieudonné-Vatran, Stefanie Krentz, Anna M Blom, Seppo Meri, Birgitta Henriques-Normark, Kristian Riesbeck, Barbara Albiger
Clinical isolates of Streptococcus pneumoniae bind the complement inhibitor C4b-binding protein in a PspC allele-dependent fashion.
J Immunol: 2009, 182(12);7865-77
[PubMed:19494311] [WorldCat.org] [DOI] (I p)Tauseef M Asmat, Katharina Klingbeil, Inga Jensch, Gerhard Burchhardt, Sven Hammerschmidt
Heterologous expression of pneumococcal virulence factor PspC on the surface of Lactococcus lactis confers adhesive properties.
Microbiology (Reading): 2012, 158(Pt 3);771-780
[PubMed:22222496] [WorldCat.org] [DOI] (I p)Sylvia Voss, Teresia Hallström, Malek Saleh, Gerhard Burchhardt, Thomas Pribyl, Birendra Singh, Kristian Riesbeck, Peter F Zipfel, Sven Hammerschmidt
The choline-binding protein PspC of Streptococcus pneumoniae interacts with the C-terminal heparin-binding domain of vitronectin.
J Biol Chem: 2013, 288(22);15614-27
[PubMed:23603906] [WorldCat.org] [DOI] (I p)Ulrike Binsker, Thomas P Kohler, Krystin Krauel, Sylvia Kohler, Hansjörg Schwertz, Sven Hammerschmidt
Pneumococcal Adhesins PavB and PspC Are Important for the Interplay with Human Thrombospondin-1.
J Biol Chem: 2015, 290(23);14542-55
[PubMed:25897078] [WorldCat.org] [DOI] (I p)Shanshan Du, Cláudia Vilhena, Samantha King, Alfredo Sahagún-Ruiz, Sven Hammerschmidt, Christine Skerka, Peter F Zipfel
Molecular analyses identifies new domains and structural differences among Streptococcus pneumoniae immune evasion proteins PspC and Hic.
Sci Rep: 2021, 11(1);1701
[PubMed:33462258] [WorldCat.org] [DOI] (I e)S Hammerschmidt, S R Talay, P Brandtzaeg, G S Chhatwal
SpsA, a novel pneumococcal surface protein with specific binding to secretory immunoglobulin A and secretory component.
Mol Microbiol: 1997, 25(6);1113-24
[PubMed:9350867] [WorldCat.org] [DOI] (P p)