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serotype 4
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serotype 6B
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serotype 6A
70585
serotype 5
A026
serotype 19F
A66
serotype 3
AP200
serotype 11A
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serotype 23F
BVJ1JL
serotype 1
CGSP14
serotype 14
G54
serotype 19F
HU-OH
serotype 3
Hu15
serotype 19A
Hu17
serotype 19A
INV104
serotype 1
INV200
serotype 14
JJA
serotype 14
MDRSPN001
serotype 19F
NCTC7466
serotype 2
NU83127
serotype 4
OXC141
serotype 3
P1031
serotype 1
R6
serotype 2
SP49
serotype 19A
SPN032672
serotype 1
SPN034156
serotype 3
SPN034183
serotype 3
SPN994038
serotype 3
SPN994039
serotype 3
SPNA45
serotype 3
ST556
serotype 19F
TCH8431/19A
serotype 19A
Taiwan19F-14
serotype 19F
Xen35
serotype 4
gamPNI0373
serotype 1

NCBI: 26-OCT-2021

⊟Summary[edit | edit source]

  • organism: Streptococcus pneumoniae R6
  • locus tag: SPR_RS00250 [old locus tag: spr0047 ]
  • pan locus tag?: PNEUPAN000692000
  • symbol: purF
  • pan gene symbol?: purF
  • synonym:
  • product: amidophosphoribosyltransferase

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SPR_RS00250 [old locus tag: spr0047 ]
  • symbol: purF
  • product: amidophosphoribosyltransferase
  • replicon: chromosome
  • strand: +
  • coordinates: 50314..51756
  • length: 1443
  • essential: unknown

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    ATGACATACGAAGTAAAATCTCTTAATGAAGAATGTGGTGTTTTCGGTATTTGGGGACAT
    CCAGATGCTGCTAAGTTGACCTATTTTGGACTCCACAGTCTTCAACACCGTGGTCAGGAG
    GGGGCAGGAATCCTCTCCAATGATCAAGGACAACTGAAGCGGCATCGCGATATGGGTCTT
    CTATCAGAAGTCTTCAGAAATCCTGCTAACTTAGATAAATTGACGGGAACTGGTGCGATT
    GGGCATGTGCGTTACGCGACTGCTGGCGAAGCTTCTGTAGATAATATCCAGCCCTTCCTC
    TTCCGTTTTCACGATATGCAGTTTGGCTTGGCTCATAATGGAAATCTGACCAATGCAGCC
    TCTCTCAAGAAAGAACTGGAACAAAGAGGAGCAATTTTCAGCGCGACTTCGGACTCTGAA
    ATCTTGGCTCACCTCATTCGTCGCAGTCATAATCCTAGCCTGATGGGCAAAATCAAGGAA
    GCGCTCAGCCTTGTCAAAGGTGGTTTTGCCTATATCTTGCTGTTTGAGGACAAGTTGATT
    GCGGCTCTTGACCCAAATGGATTCCGACCGCTTTCGATTGGTAAAATGGCTAATGGAGCA
    GTTGTTGTATCTTCTGAAACCTGTGCTTTTGAGGTCATTGGTGCCGAGTGGATTCGTGAT
    TTGAAGCCAGGTGAGATTGTGATCATTGATGACGAGGGCATTCAGTATGACAGCTATACA
    GATGATACCCAGTTGGCGGTTTGTTCTATGGAGTATATCTACTTTGCTCGCCCTGATTCT
    AATATCCACGGTGTCAATGTCCATACGGCACGTAAGAGAATGGGAGCGCAATTGGCGCGA
    GAATTTAAGCATGAGGCAGATATTGTAGTTGGTGTGCCCAATTCTTCCCTAAGCGCGGCT
    ATGGGATTTGCGGAAGAAGCAGGCTTACCAAATGAAATGGGTCTGATCAAAAACCAATAC
    ACCCAGCGAACTTTTATCCAACCGACTCAAGAATTGCGGGAGCAAGGAGTGCGGATGAAA
    CTGTCTGCTGTTTCGGGTGTTGTCAAAGGCAAACGTGTGGTCATGGTGGATGATTCCATT
    GTACGTGGAACAACCTCTCGTCGTATCGTTCAGCTCTTGAAAGAAGCGGGTGCGACTGAG
    GTTCACGTTGCCATTGGAAGTCCTGCACTAGCGTATCCATGTTTCTACGGGATTGATATC
    CAGACCCGTCAGGAGCTGATTGCAGCCAATCATACGGTCGAAGAAACTCGCCAAATCATT
    GGTGCGGACAGTATGACTTATCTTTCAATTGATGGCTTGATTGAGTCGATTGGTATCGAA
    ACAGATGCGCCGAACGGTGGTCTCTGTGTCGCTTACTTTGACGGTGACTACCCAACGCCT
    CTCTATGACTACGAAGAAGACTATCGTAGAAGTTTGGAAGAAAAGACCAGTTTTTACAAG
    TAG
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1320
    1380
    1440
    1443

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SPR_RS00250 [old locus tag: spr0047 ]
  • symbol: PurF
  • description: amidophosphoribosyltransferase
  • length: 480
  • theoretical pI: 5.46344
  • theoretical MW: 52821.5
  • GRAVY: -0.199375

⊟Function[edit | edit source]

  • reaction:
    EC 2.4.2.14?  ExPASy
    Amidophosphoribosyltransferase 5-phospho-beta-D-ribosylamine + diphosphate + L-glutamate = L-glutamine + 5-phospho-alpha-D-ribose 1-diphosphate + H2O
  • TIGRFAM:
    Metabolism Purines, pyrimidines, nucleosides, and nucleotides Purine ribonucleotide biosynthesis amidophosphoribosyltransferase (TIGR01134; EC 2.4.2.14; HMM-score: 512)
    and 14 more
    Cell structure Cell envelope Biosynthesis and degradation of murein sacculus and peptidoglycan glutamine-fructose-6-phosphate transaminase (isomerizing) (TIGR01135; EC 2.6.1.16; HMM-score: 105.5)
    Metabolism Central intermediary metabolism Amino sugars glutamine-fructose-6-phosphate transaminase (isomerizing) (TIGR01135; EC 2.6.1.16; HMM-score: 105.5)
    Metabolism Amino acid biosynthesis Aspartate family asparagine synthase (glutamine-hydrolyzing) (TIGR01536; EC 6.3.5.4; HMM-score: 58.9)
    Metabolism Purines, pyrimidines, nucleosides, and nucleotides Purine ribonucleotide biosynthesis ribose-phosphate diphosphokinase (TIGR01251; EC 2.7.6.1; HMM-score: 34.9)
    exosortase A system-associated amidotransferase 1 (TIGR03108; HMM-score: 29.2)
    Metabolism Purines, pyrimidines, nucleosides, and nucleotides Pyrimidine ribonucleotide biosynthesis orotate phosphoribosyltransferase (TIGR01367; EC 2.4.2.10; HMM-score: 21.9)
    asparagine synthase family amidotransferase (TIGR03104; HMM-score: 21.5)
    Cellular processes Cellular processes DNA transformation comF family protein (TIGR00201; HMM-score: 21.3)
    Metabolism Purines, pyrimidines, nucleosides, and nucleotides Salvage of nucleosides and nucleotides adenine phosphoribosyltransferase (TIGR01090; EC 2.4.2.7; HMM-score: 17.6)
    Metabolism Purines, pyrimidines, nucleosides, and nucleotides Salvage of nucleosides and nucleotides xanthine phosphoribosyltransferase (TIGR01744; EC 2.4.2.22; HMM-score: 15.7)
    Metabolism Purines, pyrimidines, nucleosides, and nucleotides Salvage of nucleosides and nucleotides hypoxanthine phosphoribosyltransferase (TIGR01203; EC 2.4.2.8; HMM-score: 14.9)
    Metabolism Purines, pyrimidines, nucleosides, and nucleotides Pyrimidine ribonucleotide biosynthesis orotate phosphoribosyltransferase (TIGR00336; EC 2.4.2.10; HMM-score: 12.5)
    Metabolism Purines, pyrimidines, nucleosides, and nucleotides Purine ribonucleotide biosynthesis pur operon repressor PurR (TIGR01743; HMM-score: 12.2)
    Signal transduction Regulatory functions DNA interactions pur operon repressor PurR (TIGR01743; HMM-score: 12.2)
  • TheSEED: see spr0047
  • PFAM:
    NTN (CL0052) GATase_6; Glutamine amidotransferase domain (PF13522; HMM-score: 65.2)
    GATase_7; Glutamine amidotransferase domain (PF13537; HMM-score: 64.6)
    and 6 more
    PRTase-like (CL0533) Pribosyltran; Phosphoribosyl transferase domain (PF00156; HMM-score: 40.1)
    NTN (CL0052) GATase_4; Glutamine amidotransferases class-II (PF13230; HMM-score: 31.4)
    GATase_2; Glutamine amidotransferases class-II (PF00310; HMM-score: 17.6)
    PRTase-like (CL0533) Pribosyl_synth; Phosphoribosyl synthetase-associated domain (PF14572; HMM-score: 15.1)
    NTN (CL0052) DUF3700; Aluminium induced protein (PF12481; HMM-score: 12.7)
    PRTase-like (CL0533) PRTase_2; Phosphoribosyl transferase (PF15609; HMM-score: 9.6)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 9.97
    • Cytoplasmic Membrane Score: 0
    • Cellwall Score: 0.01
    • Extracellular Score: 0.02
    • Internal Helices: 0
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.006184
    • TAT(Tat/SPI): 0.000421
    • LIPO(Sec/SPII): 0.001276
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MTYEVKSLNEECGVFGIWGHPDAAKLTYFGLHSLQHRGQEGAGILSNDQGQLKRHRDMGLLSEVFRNPANLDKLTGTGAIGHVRYATAGEASVDNIQPFLFRFHDMQFGLAHNGNLTNAASLKKELEQRGAIFSATSDSEILAHLIRRSHNPSLMGKIKEALSLVKGGFAYILLFEDKLIAALDPNGFRPLSIGKMANGAVVVSSETCAFEVIGAEWIRDLKPGEIVIIDDEGIQYDSYTDDTQLAVCSMEYIYFARPDSNIHGVNVHTARKRMGAQLAREFKHEADIVVGVPNSSLSAAMGFAEEAGLPNEMGLIKNQYTQRTFIQPTQELREQGVRMKLSAVSGVVKGKRVVMVDDSIVRGTTSRRIVQLLKEAGATEVHVAIGSPALAYPCFYGIDIQTRQELIAANHTVEETRQIIGADSMTYLSIDGLIESIGIETDAPNGGLCVAYFDGDYPTPLYDYEEDYRRSLEEKTSFYK

⊟Experimental data[edit | edit source]

  • protein localization:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

⊟Expression data[edit | edit source]

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

⊟Relevant publications[edit | edit source]

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