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serotype 4
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serotype 19A
Hu17
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INV104
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INV200
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JJA
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MDRSPN001
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NCTC7465
serotype 1
NCTC7466
serotype 2
NU83127
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OXC141
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P1031
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R6
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SP49
serotype 19A
SPN032672
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SPN034156
serotype 3
SPN034183
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SPN994038
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SPN994039
serotype 3
SPNA45
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ST556
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TCH8431/19A
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serotype 19F
Xen35
serotype 4
gamPNI0373
serotype 1

NCBI: 25-NOV-2024

⊟Summary[edit | edit source]

  • organism: Streptococcus pneumoniae R6
  • locus tag: SPR_RS03060 [old locus tag: spr0604 ]
  • pan locus tag?: PNEUPAN001604000
  • symbol: SPR_RS03060
  • pan gene symbol?: murG
  • synonym:
  • product: UDP-N-acetylglucosamine--N-acetylmuramyl- (pentapeptide) pyrophosphoryl-undecaprenol N-acetylglucosamine transferase

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SPR_RS03060 [old locus tag: spr0604 ]
  • symbol: SPR_RS03060
  • product: UDP-N-acetylglucosamine--N-acetylmuramyl- (pentapeptide) pyrophosphoryl-undecaprenol N-acetylglucosamine transferase
  • replicon: chromosome
  • strand: +
  • coordinates: 616727..617785
  • length: 1059
  • essential: yes [1] DEG other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    ATGAAAAAAATTGTCTTTACAGGTGGGGGGACGGTTGGACACGTTACCCTCAATCTTTTG
    TTAATGCCCAAGTTCATCGAAGATGGTTGGGAAGTCCACTATATCGGGGACAAGCGTGGT
    ATCGAACACCAAGAAATCCTTAAGTCAGGTTTGGATGTCACTTTCCACTCCATTGCGACT
    GGGAAATTGCGTCGCTATTTCTCTTGGCAAAATATGCTGGACGTCTTCAAAGTTGGCTGG
    GGAATCGTCCAATCGCTCTTTATCATGTTGCGACTTCGTCCACAGACCCTTTTTTCAAAG
    GGGGGCTTTGTCTCAGTACCGCCTGTTATCGCAGCGCGTGTGTCAGGAGTGCCTGTCTTT
    ATTCACGAATCTGACCTGTCTATGGGCTTGGCCAATAAAATCGCCTATAAATTTGCGACT
    AAGATGTATTCAACCTTTGAGCAAGCTTCGAGTTTGTCTAAGGTTGAGCATGTGGGAGCA
    GTGACCAAGGTTTCAGATCAAAAAAATCCAGAACCAGATGAATTGGTGGATATTCAAACC
    CACTTTAATCATAAATTGCCGACTGTATTGTTTGTTGGCGGTTCTGCAGGTGCTCGTGTC
    TTTAACCAATTGGTGACAGACCATAAGAAAGAACTAACAGAGCGCTACAATATTATCAAT
    CTAACTGGAGATTCTAGTCTGAACGAGTTGAGCCAAAATCTTTTTCGTGTTGACTATGTG
    ACCGATCTCTATCAACCCTTGATGGAATTGGCTGATATTGTTGTGACACGAGGTGGTGCC
    AATACGATTTTTGAGCTCTTGGCGATAGCAAAATTGCATGTCATTGTGCCGCTTGGTCGT
    GAAGCTAGTCGTGGTGACCAGATTGAAAATGCAGCTTACTTTGTAAAAAAAGGCTATGCA
    GAAGACCTTCAAGAAAGCGATTTGACCTTGGATAGTTTGGAAGAGAAGCTTTCTCACTTA
    CTAAGTCACAAGGAAGATTACCAAGCTAAGATGAAAGCTTCTAAGGAATTGAAATCTCTA
    GCAGATTTTTATCAATTGTTGAAAAAAGATTTATCATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1059

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SPR_RS03060 [old locus tag: spr0604 ]
  • symbol: SPR_RS03060
  • description: UDP-N-acetylglucosamine--N-acetylmuramyl- (pentapeptide) pyrophosphoryl-undecaprenol N-acetylglucosamine transferase
  • length: 352
  • theoretical pI: 7.19125
  • theoretical MW: 39448.1
  • GRAVY: -0.107102

⊟Function[edit | edit source]

  • reaction:
    EC 2.4.1.227?  ExPASy
    Undecaprenyldiphospho-muramoylpentapeptide beta-N-acetylglucosaminyltransferase UDP-N-acetylglucosamine + Mur2Ac(oyl-L-Ala-gamma-D-Glu-L-Lys-D-Ala-D-Ala)-diphosphoundecaprenol = UDP + GlcNAc-(1->4)-Mur2Ac(oyl-L-Ala-gamma-D-Glu-L-Lys-D-Ala-D-Ala)-diphosphoundecaprenol
  • TIGRFAM:
    Cell structure Cell envelope Biosynthesis and degradation of murein sacculus and peptidoglycan undecaprenyldiphospho-muramoylpentapeptide beta-N-acetylglucosaminyltransferase (TIGR01133; EC 2.4.1.227; HMM-score: 200.1)
    and 2 more
    Hypothetical proteins Conserved conserved hypothetical protein (TIGR00661; HMM-score: 29.4)
    Cellular processes Cellular processes Toxin production and resistance glycosyltransferase, MGT family (TIGR01426; EC 2.4.1.-; HMM-score: 22.4)
  • TheSEED: see spr0604
  • PFAM:
    GT-B (CL0113) Glyco_transf_28; Glycosyltransferase family 28 N-terminal domain (PF03033; HMM-score: 146)
    Glyco_tran_28_C; Glycosyltransferase family 28 C-terminal domain (PF04101; HMM-score: 123)
    and 1 more
    UDPGT; UDP-glucoronosyl and UDP-glucosyl transferase (PF00201; HMM-score: 14.3)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic Membrane
    • Cytoplasmic Score: 1.05
    • Cytoplasmic Membrane Score: 8.78
    • Cellwall Score: 0.08
    • Extracellular Score: 0.09
    • Internal Helices: 0
  • DeepLocPro: Cytoplasmic Membrane
    • Cytoplasmic Score: 0.0376
    • Cytoplasmic Membrane Score: 0.9355
    • Cell wall & surface Score: 0.0006
    • Extracellular Score: 0.0263
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.041869
    • TAT(Tat/SPI): 0.000374
    • LIPO(Sec/SPII): 0.00515
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MKKIVFTGGGTVGHVTLNLLLMPKFIEDGWEVHYIGDKRGIEHQEILKSGLDVTFHSIATGKLRRYFSWQNMLDVFKVGWGIVQSLFIMLRLRPQTLFSKGGFVSVPPVIAARVSGVPVFIHESDLSMGLANKIAYKFATKMYSTFEQASSLSKVEHVGAVTKVSDQKNPEPDELVDIQTHFNHKLPTVLFVGGSAGARVFNQLVTDHKKELTERYNIINLTGDSSLNELSQNLFRVDYVTDLYQPLMELADIVVTRGGANTIFELLAIAKLHVIVPLGREASRGDQIENAAYFVKKGYAEDLQESDLTLDSLEEKLSHLLSHKEDYQAKMKASKELKSLADFYQLLKKDLS

⊟Experimental data[edit | edit source]

  • protein localization:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator:

⊟Transcription[edit | edit source]

  • transcription start site:

⊟Expression data[edit | edit source]

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You can add further information about the gene and protein here. [edit]

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ Jane A Thanassi, Sandra L Hartman-Neumann, Thomas J Dougherty, Brian A Dougherty, Michael J Pucci
    Identification of 113 conserved essential genes using a high-throughput gene disruption system in Streptococcus pneumoniae.
    Nucleic Acids Res: 2002, 30(14);3152-62
    [PubMed:12136097] [WorldCat.org] [DOI] (I p)

⊟Relevant publications[edit | edit source]

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