Jump to navigation
Jump to search
PangenomeTIGR4
serotype 4
D39serotype 2
D39Vserotype 2
Hungary19A-6serotype 19A
EF3030serotype 19F
670-6Bserotype 6B
6A-10serotype 6A
70585serotype 5
A026serotype 19F
A66serotype 3
AP200serotype 11A
ASP0581serotype 12F
ATCC 49619serotype 19F
ATCC 700669serotype 23F
BVJ1JLserotype 1
CGSP14serotype 14
G54serotype 19F
HU-OHserotype 3
Hu15serotype 19A
Hu17serotype 19A
INV104serotype 1
INV200serotype 14
JJAserotype 14
MDRSPN001serotype 19F
NCTC7466serotype 2
NU83127serotype 4
OXC141serotype 3
P1031serotype 1
R6serotype 2
SP49serotype 19A
SPN032672serotype 1
SPN034156serotype 3
SPN034183serotype 3
SPN994038serotype 3
SPN994039serotype 3
SPNA45serotype 3
ST556serotype 19F
TCH8431/19Aserotype 19A
Taiwan19F-14serotype 19F
Xen35serotype 4
gamPNI0373serotype 1
NCBI: 09-NOV-2021
⊟Summary[edit | edit source]
- organism: Streptococcus pneumoniae Taiwan19F-14
- locus tag: SPT_RS04235 [old locus tag: SPT_0854 ]
- pan locus tag?: PNEUPAN002746000
- symbol: nadE
- pan gene symbol?: nadE
- synonym:
- product: ammonia-dependent NAD(+) synthetase
⊟Genome View[edit | edit source]
⊟Gene[edit | edit source]
⊟General[edit | edit source]
- type: CDS
- locus tag: SPT_RS04235 [old locus tag: SPT_0854 ]
- symbol: nadE
- product: ammonia-dependent NAD(+) synthetase
- replicon: chromosome
- strand: +
- coordinates: 802571..803395
- length: 825
- essential: unknown other strains
⊟Accession numbers[edit | edit source]
- Location: NC_012469 (802571..803395) NCBI
- BioCyc: SPT_RS04235 BioCyc
- MicrobesOnline: see SPT_0854
- PneumoBrowse for strain D39V: SPV_1250 PneumoBrowse
⊟Phenotype[edit | edit source]
Share your knowledge and add information here. [edit]
⊟DNA sequence[edit | edit source]
- 1
61
121
181
241
301
361
421
481
541
601
661
721
781ATGAGTTTGCAAGAAACGATTATCCAAGAGCTGGGTGTCAAACCAGTGATTGATGCCCAG
GAAGAAATCCGTCGTTCTATTGATTTCTTAAAAAGATATCTGAAAAAACATCCCTTCCTA
AAAACCTTTGTACTAGGGATTTCTGGGGGACAAGACTCAACCTTGGCAGGACGTTTGGCG
CAATTAGCTATGGAAGAACTGCGAGCTGAAACGGGAGACGATAGCTACAAATTTATCGCT
GTCCGCCTGCCATACGGAGTGCAAGCTGATGAAGCAGATGCTCAAAAAGCCCTAGCCTTC
ATCCAGCCAGATGTCAGCTTGGTTGTGAATATCAAGGAATCAGCTGATGCCATGACAGCT
GCAGTTGAAGCGACAGGTAGTCCTGTTTCAGACTTCAACAAGGGGAATATCAAGGCACGT
TGCCGTATGATTGCTCAGTATGCCCTTGCTGGTTCCCATAGCGGAGCGGTCATTGGAACA
GACCACGCCGCGGAAAATATCACAGGTTTCTTTACCAAGTTTGGTGACGGCGGTGCGGAT
ATTCTCCCTCTTTACCGCCTCAATAAACGCCAAGGAAAACAGCTCTTGCAGAAACTTGGC
GCAGAGCCAGCCCTTTATGAAAAAATCCCAACGGCAGACCTAGAAGAAGATAAACCAGGC
CTAGCTGACGAAGTCGCACTTGGAGTCACCTACGCAGAGATTGACGACTACCTAGAAGGC
AAAACAATCAGCCCAGAAGCTCAAGCGACCATTGAAAACTGGTGGCACAAAGGCCAACAC
AAACGCCACTTACCCATCACCGTATTTGATGACTTTTGGGAGTAA60
120
180
240
300
360
420
480
540
600
660
720
780
825
⊟Protein[edit | edit source]
⊟General[edit | edit source]
- locus tag: SPT_RS04235 [old locus tag: SPT_0854 ]
- symbol: NadE
- description: ammonia-dependent NAD(+) synthetase
- length: 274
- theoretical pI: 4.86022
- theoretical MW: 30134.9
- GRAVY: -0.268613
⊟Function[edit | edit source]
- reaction: EC 6.3.1.5? ExPASyNAD+ synthase ATP + deamido-NAD+ + NH3 = AMP + diphosphate + NAD+
- TIGRFAM: Biosynthesis of cofactors, prosthetic groups, and carriers Pyridine nucleotides NAD+ synthetase (TIGR00552; EC 6.3.1.5; HMM-score: 239.2)
- TheSEED: see SPT_0854
- PFAM: HUP (CL0039) NAD_synthase; NAD synthase (PF02540; HMM-score: 227.8)
⊟Structure, modifications & cofactors[edit | edit source]
- domains:
- modifications:
- cofactors:
- effectors:
⊟Localization[edit | edit source]
- PSORTb: Cytoplasmic
- Cytoplasmic Score: 7.5
- Cytoplasmic Membrane Score: 1.15
- Cellwall Score: 0.62
- Extracellular Score: 0.73
- Internal Helices: 0
- SignalP: no predicted signal peptide
- SP(Sec/SPI): 0.010183
- TAT(Tat/SPI): 0.011435
- LIPO(Sec/SPII): 0.001198
- predicted transmembrane helices (TMHMM): 0
⊟Accession numbers[edit | edit source]
⊟Protein sequence[edit | edit source]
- MSLQETIIQELGVKPVIDAQEEIRRSIDFLKRYLKKHPFLKTFVLGISGGQDSTLAGRLAQLAMEELRAETGDDSYKFIAVRLPYGVQADEADAQKALAFIQPDVSLVVNIKESADAMTAAVEATGSPVSDFNKGNIKARCRMIAQYALAGSHSGAVIGTDHAAENITGFFTKFGDGGADILPLYRLNKRQGKQLLQKLGAEPALYEKIPTADLEEDKPGLADEVALGVTYAEIDDYLEGKTISPEAQATIENWWHKGQHKRHLPITVFDDFWE
⊟Experimental data[edit | edit source]
- protein localization:
- interaction partners:
⊟Expression & Regulation[edit | edit source]
⊟Operon[edit | edit source]
⊟Regulation[edit | edit source]
- regulator: Rex* see SPT_0854
⊟Expression data[edit | edit source]
- PneumoExpress for strain D39V: SPV_1250 PneumoExpress
⊟Biological Material[edit | edit source]
⊟Mutants[edit | edit source]
⊟Expression vector[edit | edit source]
⊟lacZ fusion[edit | edit source]
⊟GFP fusion[edit | edit source]
⊟two-hybrid system[edit | edit source]
⊟FLAG-tag construct[edit | edit source]
⊟Antibody[edit | edit source]
⊟Other Information[edit | edit source]
You are kindly invited to share additional interesting facts.