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PangenomeTIGR4
serotype 4
D39
serotype 2
D39V
serotype 2
Hungary19A-6
serotype 19A
EF3030
serotype 19F
670-6B
serotype 6B
6A-10
serotype 6A
70585
serotype 5
A026
serotype 19F
A66
serotype 3
AP200
serotype 11A
ASP0581
serotype 12F
ATCC 49619
serotype 19F
ATCC 700669
serotype 23F
BVJ1JL
serotype 1
CGSP14
serotype 14
G54
serotype 19F
HU-OH
serotype 3
Hu15
serotype 19A
Hu17
serotype 19A
INV104
serotype 1
INV200
serotype 14
JJA
serotype 14
MDRSPN001
serotype 19F
NCTC7466
serotype 2
NU83127
serotype 4
OXC141
serotype 3
P1031
serotype 1
R6
serotype 2
SP49
serotype 19A
SPN032672
serotype 1
SPN034156
serotype 3
SPN034183
serotype 3
SPN994038
serotype 3
SPN994039
serotype 3
SPNA45
serotype 3
ST556
serotype 19F
TCH8431/19A
serotype 19A
Taiwan19F-14
serotype 19F
Xen35
serotype 4
gamPNI0373
serotype 1

NCBI: 31-JAN-2014

⊟Summary[edit | edit source]

  • organism: Streptococcus pneumoniae TIGR4
  • locus tag: SP_0032 [new locus tag: SP_RS00195 ]
  • pan locus tag?: PNEUPAN000656000
  • symbol: polA
  • pan gene symbol?: polA
  • synonym:
  • product: DNA polymerase I

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SP_0032 [new locus tag: SP_RS00195 ]
  • symbol: polA
  • product: DNA polymerase I
  • replicon: chromosome
  • strand: +
  • coordinates: 31535..34168
  • length: 2634
  • essential: yes [1]

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    ATGGATAAGAAAAAATTATTATTGATTGATGGGTCTTCTGTAGCTTTTCGGGCGTTTTTT
    GCGCTGTATCAGCAGTTGGACCGTTTTAAGAATGTGGCTGGTTTGCATACCAATGCGATT
    TATGGTTTTCAGTTGATGTTGAGTCATTTATTGGAGCGGGTTGAGCCGAGTCATATTTTG
    GTGGCTTTTGATGCGGGAAAGACGACCTTCCGGACAGAGATGTATGCGGACTATAAGGGT
    GGTCGGGCCAAGACTCCTGATGAGTTTCGTGAGCAATTTCCTTTCATTCGTGAGTTGCTG
    GATCATATGGGGATTCGTCACTATGATCTGGCTCAGTATGAGGCGGATGACATCATTGGG
    ACGCTGGATAAGCTAGCAGAGCAGGATGGTTTTGATATTACTATTGTCAGTGGGGACAAG
    GATTTGATTCAGCTGACGGATGAGCATACGGTGGTTGAAATTTCCAAGAAAGGTGTGGCT
    GAGTTTGAGGCCTTTACGCCAGATTACCTCATGGAAGAAATGGGCCTCACACCAGCTCAG
    TTTATCGATCTCAAGGCGCTCATGGGTGATAAGTCGGATAATATCCCTGGGGTGACCAAA
    GTCGGTGAAAAGACGGGTATTAAGCTCTTGCTGGAGCATGGTTCGCTTGAGGGGATTTAT
    GAAAATATTGATGGAATGAAGACTTCTAAGATGAAGGAAAATCTCATCAATGACAAGGAA
    CAGGCCTTTTTGTCTAAAACACTAGCGACCATTGATACCAAGGCACCGATTGCGATTGGT
    TTAGAGGACTTGGTCTATAGTGGTCCAGATGTTGAAAATCTTGGGAAATTCTACGATGAG
    ATGGGCTTCAAACAGCTAAAGCAGGCTTTAAATGTGTCGTCAGCTGATGTGTCTGAGAGT
    TTGGATTTTACTATTGTTGACCAAATCAGTCAAGATATGCTGAGTGAAGAGTCTATCTTC
    CACTTTGAGCTTTTTGGTGAGAATTACCATACGGATAATTTGGTTGGATTTGTCTGGTCT
    TGTGGGGATAAGCTCTATGCCACAGACAAGCTTGAGCTGTTGCAAGACCCGATTTTCAAG
    GATTTCTTAGAAAAAACATCTCTGAGAGTTTATGACTTTAAGAAGGTTAAAGTTCTTTTG
    CAACGTTTTGGTGTGGATTTGCAGGCGCCTGCTTTTGACATCCGTTTGGCTAAATACCTC
    CTTTCGACTGTGGAGGACAATGAAATTGCGACCATCGCTAGTCTTTATGGTCAGACTTAC
    TTGGTTGATGATGAAACTTTCTACGGTAAGGGTGTTAAAAAGGCCATTCCTGAACGTGAG
    AAATTCTTGGAACACTTAGCTTGTAAACTTGCTGTTTTGGTAGAAACAGAGCCTATTTTA
    CTTGAAAAACTCAGCGAAAATGGGCAATTAGAGCTTCTTTATGATATGGAGCAACCTCTG
    GCTTTTGTCCTTGCCAAGATGGAAATTGCTGGGATTATGGTCAAGAAAGAGACCTTGCTT
    GAGATGCAGGCTGAAAATGAGCTTGTCATTGAAAAACTGACTCAAGAGATTTACGAGCTG
    GCTGGTGAGGAGTTTAATGTCAACTCGCCTAAGCAGTTGGGCGTGCTTCTCTTTGAGAAA
    TTGGGACTTCCTCTAGAATACACTAAGAAAACCAAGACAGGTTATTCGACAGCAGTGGAT
    GTTTTAGAGCGTCTCGCTCCTATTGCTCCGATTGTTAAGAAAATCCTGGATTACCGTCAA
    ATTGCTAAGATTCAATCTACTTATGTAATTGGCTTGCAGGACTGGATTTTGGCTGATGGA
    AAGATTCATACTCGCTATGTGCAGGATTTGACCCAGACCGGGCGTTTGTCTAGTGTGGAT
    CCAAACTTGCAAAATATTCCTGCCCGATTGGAACAGGGGCGCTTGATTCGGAAGGCTTTT
    GTGCCAGAGTGGGAGGATAGTGTGCTACTCAGCTCTGACTATTCACAGATTGAATTGCGC
    GTTTTGGCGCATATTTCTAAGGATGAGCACTTGATTAAGGCCTTCCAAGAGGGGGCAGAT
    ATCCATACTTCGACAGCCATGCGGGTCTTTGGCATTGAGCGTCCTGATGATGTGACTGCA
    AACGACCGTCGCAATGCCAAGGCAGTTAACTTTGGAGTGGTTTATGGGATTTCAGACTTT
    GGCTTGTCTAATAATTTGGGAATTAGTCGTAAGGAAGCCAAAGCCTACATTGATACCTAC
    TTTGAACGTTTTCCAGGTATTAAAAACTACATGGATGAAGTGGTGCGGGAGGCGCGTGAT
    AAGGGCTATGTAGAGACCCTCTTTAAGCGTCGCCGTGAGTTGCCAGATATCAATTCGCGC
    AACTTCAATATTCGTGGTTTTGCGGAGCGAACTGCTATCAACTCACCTATCCAGGGTTCG
    GCAGCAGATATTCTCAAGATTGCCATGATTCAGCTGGATAAAGCCTTGGTTGCAGGTGGT
    TATCAGACTAAGATGCTGTTACAAGTGCACGATGAAATCGTCCTTGAAGTGCCTAAATCT
    GAATTGGTAGAGATGAAAAAATTGGTGAAACAAACCATGGAAGAAGCCATTCAACTCAGT
    GTTCCTCTTATCGCAGATGAGAATGAAGGGGCAACCTGGTACGAGGCTAAATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1320
    1380
    1440
    1500
    1560
    1620
    1680
    1740
    1800
    1860
    1920
    1980
    2040
    2100
    2160
    2220
    2280
    2340
    2400
    2460
    2520
    2580
    2634

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SP_0032 [new locus tag: SP_RS00195 ]
  • symbol: PolA
  • description: DNA polymerase I
  • length: 877
  • theoretical pI: 4.70539
  • theoretical MW: 99252
  • GRAVY: -0.210718

⊟Function[edit | edit source]

  • reaction:
    EC 2.7.7.7?  ExPASy
    DNA-directed DNA polymerase Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1)
  • TIGRFAM:
    Genetic information processing DNA metabolism DNA replication, recombination, and repair DNA polymerase I (TIGR00593; EC 2.7.7.7; HMM-score: 1041.6)
    and 1 more
    flap structure-specific endonuclease (TIGR03674; EC 3.1.-.-; HMM-score: 17.8)
  • TheSEED  :
    • DNA polymerase I (EC 2.7.7.7)
    DNA Metabolism DNA repair DNA Repair Base Excision  DNA polymerase I (EC 2.7.7.7)
    and 1 more
    DNA Metabolism DNA replication DNA-replication  DNA polymerase I (EC 2.7.7.7)
  • PFAM:
    no clan defined DNA_pol_A; DNA polymerase family A (PF00476; HMM-score: 498.7)
    and 3 more
    PIN (CL0280) 5_3_exonuc_N; 5'-3' exonuclease, N-terminal resolvase-like domain (PF02739; HMM-score: 163.6)
    5_3_exonuc_C (CL0464) 5_3_exonuc; 5'-3' exonuclease, C-terminal SAM fold (PF01367; HMM-score: 113.5)
    no clan defined Mt_ATP_synt; Mitochondrial ATP synthase subunit (PF15704; HMM-score: 10.9)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.003043
    • TAT(Tat/SPI): 0.000434
    • LIPO(Sec/SPII): 0.000965
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MDKKKLLLIDGSSVAFRAFFALYQQLDRFKNVAGLHTNAIYGFQLMLSHLLERVEPSHILVAFDAGKTTFRTEMYADYKGGRAKTPDEFREQFPFIRELLDHMGIRHYDLAQYEADDIIGTLDKLAEQDGFDITIVSGDKDLIQLTDEHTVVEISKKGVAEFEAFTPDYLMEEMGLTPAQFIDLKALMGDKSDNIPGVTKVGEKTGIKLLLEHGSLEGIYENIDGMKTSKMKENLINDKEQAFLSKTLATIDTKAPIAIGLEDLVYSGPDVENLGKFYDEMGFKQLKQALNVSSADVSESLDFTIVDQISQDMLSEESIFHFELFGENYHTDNLVGFVWSCGDKLYATDKLELLQDPIFKDFLEKTSLRVYDFKKVKVLLQRFGVDLQAPAFDIRLAKYLLSTVEDNEIATIASLYGQTYLVDDETFYGKGVKKAIPEREKFLEHLACKLAVLVETEPILLEKLSENGQLELLYDMEQPLAFVLAKMEIAGIMVKKETLLEMQAENELVIEKLTQEIYELAGEEFNVNSPKQLGVLLFEKLGLPLEYTKKTKTGYSTAVDVLERLAPIAPIVKKILDYRQIAKIQSTYVIGLQDWILADGKIHTRYVQDLTQTGRLSSVDPNLQNIPARLEQGRLIRKAFVPEWEDSVLLSSDYSQIELRVLAHISKDEHLIKAFQEGADIHTSTAMRVFGIERPDDVTANDRRNAKAVNFGVVYGISDFGLSNNLGISRKEAKAYIDTYFERFPGIKNYMDEVVREARDKGYVETLFKRRRELPDINSRNFNIRGFAERTAINSPIQGSAADILKIAMIQLDKALVAGGYQTKMLLQVHDEIVLEVPKSELVEMKKLVKQTMEEAIQLSVPLIADENEGATWYEAK

⊟Experimental data[edit | edit source]

  • protein localization:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator:

⊟Expression data[edit | edit source]

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ Tim van Opijnen, Andrew Camilli
    A fine scale phenotype-genotype virulence map of a bacterial pathogen.
    Genome Res: 2012, 22(12);2541-51
    [PubMed:22826510] [WorldCat.org] [DOI] (I p)

⊟Relevant publications[edit | edit source]

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