From PneumoWiki
Jump to navigation Jump to search
PangenomeTIGR4
serotype 4
D39
serotype 2
D39V
serotype 2
Hungary19A-6
serotype 19A
EF3030
serotype 19F
670-6B
serotype 6B
6A-10
serotype 6A
70585
serotype 5
A026
serotype 19F
A66
serotype 3
AP200
serotype 11A
ASP0581
serotype 12F
ATCC 49619
serotype 19F
ATCC 700669
serotype 23F
BM6001
serotype 19F
BVJ1JL
serotype 1
CGSP14
serotype 14
G54
serotype 19F
HU-OH
serotype 3
Hu15
serotype 19A
Hu17
serotype 19A
INV104
serotype 1
INV200
serotype 14
JJA
serotype 14
MDRSPN001
serotype 19F
NCTC7465
serotype 1
NCTC7466
serotype 2
NU83127
serotype 4
OXC141
serotype 3
P1031
serotype 1
R6
serotype 2
SP49
serotype 19A
SPN032672
serotype 1
SPN034156
serotype 3
SPN034183
serotype 3
SPN994038
serotype 3
SPN994039
serotype 3
SPNA45
serotype 3
ST556
serotype 19F
TCH8431/19A
serotype 19A
Taiwan19F-14
serotype 19F
Xen35
serotype 4
gamPNI0373
serotype 1

NCBI: 31-JAN-2014

⊟Summary[edit | edit source]

  • organism: Streptococcus pneumoniae TIGR4
  • locus tag: SP_0074 [new locus tag: SP_RS00390 ]
  • pan locus tag?: PNEUPAN000700000
  • symbol: SP_0074
  • pan gene symbol?: maa
  • synonym:
  • product: acetyltransferase, CysE/LacA/LpxA/NodL family

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SP_0074 [new locus tag: SP_RS00390 ]
  • symbol: SP_0074
  • product: acetyltransferase, CysE/LacA/LpxA/NodL family
  • replicon: chromosome
  • strand: +
  • coordinates: 80647..81204
  • length: 558
  • essential: unknown

⊟Accession numbers[edit | edit source]

  • Location: AE005672 (80647..81204) NCBI
  • BioCyc:
  • MicrobesOnline: 115622 MicrobesOnline
  • PneumoBrowse for strain D39V:

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    ATGACTAGCGAATACCAGAAAATGATAGCAGGAGAGTTTTACCGTCCATCAGACCCAGAA
    CTGAGAGCCTTAGCTCAGGCTTCTCGCCAAAAACAGGCCGCCTTTAACAAGGAAGAGAAC
    CCCTTGAAGGGAGCCGAAATCATCAAGACTTGGTTTGCCTCAACCGGGAAAAATCTTTAC
    ATCAACACTCGCTTGATGGTGGACTACGGTGTCAACATCCATCTAGGGGAAAATTTTTAT
    TCTAATTGGAACTTGACCATGCTGGATATCTGTCCCATTCGTATCGGGGACAATGCTATG
    ATTGGTCCTAATTGTCAGTTTTTGACACCCCTCCATCCACTAGATCCACAGGAACGCAAT
    TCAGGTATCGAGTACGGAAAGCCTATCACAATCGGAGATAATTTCTGGACTGGTGGTGGC
    GTCATTGTCCTTCCTGGAGTGACACTGGGAAATAATGTCGTTGCAGGAGCAGGGGCAGTA
    ATTACCAAATCTTTTGGCGACAACGTTGTCCTAGCTGGCAATCCTGCGCGCGTGATTAAG
    GAAATACCTGTTAAATAG
    60
    120
    180
    240
    300
    360
    420
    480
    540
    558

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SP_0074 [new locus tag: SP_RS00390 ]
  • symbol: SP_0074
  • description: acetyltransferase, CysE/LacA/LpxA/NodL family
  • length: 185
  • theoretical pI: 7.4089
  • theoretical MW: 20238.1
  • GRAVY: -0.153514

⊟Function[edit | edit source]

  • TIGRFAM:
    colanic acid biosynthesis acetyltransferase WcaF (TIGR04008; EC 2.3.1.-; HMM-score: 56)
    2,3,4,5-tetrahydropyridine-2,6-dicarboxylate N-acetyltransferase (TIGR03532; EC 2.3.1.89; HMM-score: 47.4)
    phosphonate metabolim protein, transferase hexapeptide repeat family (TIGR03308; HMM-score: 46)
    sugar O-acyltransferase, sialic acid O-acetyltransferase NeuD family (TIGR03570; HMM-score: 45.7)
    and 8 more
    colanic acid biosynthesis acetyltransferase WcaB (TIGR04016; EC 2.3.1.-; HMM-score: 28.9)
    Metabolism Amino acid biosynthesis Serine family serine O-acetyltransferase (TIGR01172; EC 2.3.1.30; HMM-score: 26.2)
    Cell structure Cell envelope Biosynthesis and degradation of surface polysaccharides and lipopolysaccharides UDP-3-O-[3-hydroxymyristoyl] glucosamine N-acyltransferase LpxD (TIGR01853; EC 2.3.1.191; HMM-score: 19.3)
    Cell structure Cell envelope Biosynthesis and degradation of surface polysaccharides and lipopolysaccharides UDP-N-acetylglucosamine diphosphorylase/glucosamine-1-phosphate N-acetyltransferase (TIGR01173; EC 2.3.1.157,2.7.7.23; HMM-score: 15.4)
    Cell structure Cell envelope Biosynthesis and degradation of murein sacculus and peptidoglycan UDP-N-acetylglucosamine diphosphorylase/glucosamine-1-phosphate N-acetyltransferase (TIGR01173; EC 2.3.1.157,2.7.7.23; HMM-score: 15.4)
    Metabolism Central intermediary metabolism Amino sugars UDP-N-acetylglucosamine diphosphorylase/glucosamine-1-phosphate N-acetyltransferase (TIGR01173; EC 2.3.1.157,2.7.7.23; HMM-score: 15.4)
    Metabolism Transport and binding proteins Anions sulfate ABC transporter, sulfate-binding protein (TIGR00971; HMM-score: 11)
    non-ribosomal peptide synthetase terminal domain of unknown function (TIGR02353; HMM-score: 10.8)
  • TheSEED  :
    • Maltose O-acetyltransferase (EC 2.3.1.79)
    Carbohydrates Di- and oligosaccharides Maltose and Maltodextrin Utilization  Maltose O-acetyltransferase (EC 2.3.1.79)
  • PFAM:
    no clan defined Mac; Maltose acetyltransferase (PF12464; HMM-score: 38.7)
    HEXAPEP (CL0536) Hexapep; Bacterial transferase hexapeptide (six repeats) (PF00132; HMM-score: 36.4)
    Hexapep_2; Hexapeptide repeat of succinyl-transferase (PF14602; HMM-score: 32.4)
    and 2 more
    EAD1 (CL0699) EAD10; Effector-associated domain 10 (PF19954; HMM-score: 13.3)
    no clan defined DUF6767; Domain of unknown function (DUF6767) (PF20555; HMM-score: 12.6)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 9.67
    • Cytoplasmic Membrane Score: 0.01
    • Cellwall Score: 0.15
    • Extracellular Score: 0.17
    • Internal Helices: 0
  • DeepLocPro: Cytoplasmic
    • Cytoplasmic Score: 0.9985
    • Cytoplasmic Membrane Score: 0.0001
    • Cell wall & surface Score: 0
    • Extracellular Score: 0.0014
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.014071
    • TAT(Tat/SPI): 0.001798
    • LIPO(Sec/SPII): 0.001638
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MTSEYQKMIAGEFYRPSDPELRALAQASRQKQAAFNKEENPLKGAEIIKTWFASTGKNLYINTRLMVDYGVNIHLGENFYSNWNLTMLDICPIRIGDNAMIGPNCQFLTPLHPLDPQERNSGIEYGKPITIGDNFWTGGGVIVLPGVTLGNNVVAGAGAVITKSFGDNVVLAGNPARVIKEIPVK

⊟Experimental data[edit | edit source]

  • protein localization:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator:

⊟Transcription[edit | edit source]

  • transcription start site: 80628 [1]

⊟Expression data[edit | edit source]

  • PneumoExpress for strain D39V:

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You can add further information about the gene and protein here. [edit]

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ 1.0 1.1 Indu Warrier, Nikhil Ram-Mohan, Zeyu Zhu, Ariana Hazery, Haley Echlin, Jason Rosch, Michelle M Meyer, Tim van Opijnen
    The Transcriptional landscape of Streptococcus pneumoniae TIGR4 reveals a complex operon architecture and abundant riboregulation critical for growth and virulence.
    PLoS Pathog: 2018, 14(12);e1007461
    [PubMed:30517198] [WorldCat.org] [DOI] (I e)

⊟Relevant publications[edit | edit source]

Retrieved from "http://pneumowiki.med.uni-greifswald.de/index.php?title=SP_0074&oldid=2294"