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PangenomeTIGR4
serotype 4
D39
serotype 2
D39V
serotype 2
Hungary19A-6
serotype 19A
EF3030
serotype 19F
670-6B
serotype 6B
6A-10
serotype 6A
70585
serotype 5
A026
serotype 19F
A66
serotype 3
AP200
serotype 11A
ASP0581
serotype 12F
ATCC 49619
serotype 19F
ATCC 700669
serotype 23F
BVJ1JL
serotype 1
CGSP14
serotype 14
G54
serotype 19F
HU-OH
serotype 3
Hu15
serotype 19A
Hu17
serotype 19A
INV104
serotype 1
INV200
serotype 14
JJA
serotype 14
MDRSPN001
serotype 19F
NCTC7466
serotype 2
NU83127
serotype 4
OXC141
serotype 3
P1031
serotype 1
R6
serotype 2
SP49
serotype 19A
SPN032672
serotype 1
SPN034156
serotype 3
SPN034183
serotype 3
SPN994038
serotype 3
SPN994039
serotype 3
SPNA45
serotype 3
ST556
serotype 19F
TCH8431/19A
serotype 19A
Taiwan19F-14
serotype 19F
Xen35
serotype 4
gamPNI0373
serotype 1

NCBI: 31-JAN-2014

⊟Summary[edit | edit source]

  • organism: Streptococcus pneumoniae TIGR4
  • locus tag: SP_0895 [new locus tag: SP_RS04415 ]
  • pan locus tag?: PNEUPAN001891000
  • symbol: dnaE
  • pan gene symbol?: dnaE
  • synonym:
  • product: DNA polymerase III, alpha subunit

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SP_0895 [new locus tag: SP_RS04415 ]
  • symbol: dnaE
  • product: DNA polymerase III, alpha subunit
  • replicon: chromosome
  • strand: +
  • coordinates: 847061..850189
  • length: 3129
  • essential: yes [1]

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    GTGAGAAGGATAGAGGAGAAGTGTAAATTGATCGCACAACTAGATACAAAAACAGTCTAT
    AGTTTTATGGAAAGCGTCATTTCGATCGAAAAGTATGTGAGAGCAGCTAAAGAATACGGC
    TACACTCATTTGGCTATGATGGATATTGACAATCTTTATGGCGCTTTCGACTTTCTAGAG
    ATTACAAAAAAATACGGCATTCATCCTTTGCTAGGGCTTGAAATGACAGTGTTTGTAGAT
    GATCAGGGAGTGAATTTGCGCTTTTTAGCTCTATCTAGTGTGGGCTATCAGCAGTTGATG
    AAGCTTTCGACAGCCAAGATGCAGGGGGAGAAAACTTGGTCAGTCCTGTCCCAGTACCTG
    GAGGATATCGCGGTCATTGTGCCTTATTTTGATAGAGTTGAGTCGTTAGAACTAGGCTGT
    GATTACTATATAGGGGTTTATCCAGAAACACTAGCAAGCGAATTTCATCATCCTATCTTA
    CCTCTTTATCGGGTCAACGCTTTTGAAAGCAGGGATAGAGAAGTTCTTCAAGTTTTAACA
    GCGATTAAAGAAAATCTACCGCTCAGAGAAGTTCCCTTGCGTTCGAGACAAGATGTCTTT
    ATATCAGCAAGTTCTTTAGAGAAACTATTCCAAGAGCGTTTTCCGCAAGCTTTGGACAAT
    TTAGAAAAGCTTATTTCAGGCATTTCTTACGACTTGGATACTAGTCTGAAACTGCCTCGT
    TTTAATCCAGCTAGACCAGCAGTAGAGGAGTTGAGAGAGCGTGCTGAACTGGGGCTTGTT
    CAGAAGGGGTTGACTAGTAAAGAATATCAAGATAGACTAGACCAAGAATTGTCTGTTATT
    CATGATATGGGCTTTGATGATTATTTCTTGGTTGTTTGGGATTTGTTGCGTTTTGGACAA
    TCGAATGGCTATTATATGGGAATGGGAAGGGGTTCTGCAGTAGGCAGTTTGGTTTCTTAT
    GCCTTAGACATCACGGGGATTGACCCAGTAGAGAAAAATCTGATTTTTGAACGCTTTCTT
    AATCGTGAACGCTATACCATGCCTGATATTGATATTGATATCCCAGATATTTATCGTCCA
    GATTTTATCAGATATGTTGGTAATAAATATGGTAGTAAACATGCGGCACAAATCGTTACT
    TTTTCAACCTTTGGAGCCAAGCAAGCTCTTCGAGATGTCTTGAAACGCTTTGGTGTGCCA
    GAGTATGAATTATCTGCAATTACTAAGAAAATCAGTTTTCGTGACAATCTTAAGTCGGCC
    TATGAGGGAAATCTCCAGTTTCGTCAGCAAATCAATAGTAAGTTAGAATACCAAAAAGCT
    TTTGAGATTGCTTGCAAGATAGAGGGCTATCCAAGGCAAACCTCTGTCCATGCGGCTGGT
    GTTGTAATTAGTGACCAAGATTTAACCAACTACATTCCTCTAAAGTATGGTGATGAAATT
    CCACTGACTCAGTATGATGCTCATGGAGTTGAGGCTAGCGGACTTTTGAAGATGGACTTT
    CTGGGACTACGAAATTTGACCTTTGTCCAGAAGATGCAAGAGTTGCTTGCTGAAACAGAA
    GGTATTCATCTGAAAATTGAAGAAATCGATTTAGAAGACAAAGAAACGTTAGCTTTATTT
    GCCTCTGGTAATACAAAAGGTATCTTTCAATTTGAGCAACCAGGTGCCATTCGTCTGCTT
    AAGCGTGTGCAACCAGTCTGTTTTGAAGATGTCGTCGCGACTACTTCTCTAAATCGACCG
    GGTGCTAGTGACTATATCAATAATTTTGTGGCAAGAAAGCATGGGCAGGAAGAAGTGACT
    GTTCTGGATCCAGTACTGGAGGATATTTTGGCTCCAACCTACGGCATAATGCTCTATCAG
    GAGCAGGTTATGCAGGTTGCCCAGCGACTTGCCGGATTTAGTCTTGGGAAAGCCGATATT
    TTGCGTCGGGCTATGGGGAAAAAGGATGCCTCTGCCATGCATGAGATGAGGGCTTCCTTT
    ATTCAAGGTTCATTAGAAGCTGGTCATACTGTGGAAAAAGCAGAGCAGGTCTTTGATGTT
    ATGGAGAAGTTTGCAGGTTATGGTTTTAACAGGTCACACGCCTATGCCTACTCAGCCTTG
    GCCTTCCAGTTGGCTTATTTCAAAACGCATTATCCAGCCATTTTTTATCAGGTCATGTTA
    AATTCTTCCAACAGTGATTACTTAATAGATGCACTTGAAGCAGGTTTTGAAGTAGCCTCT
    CTATCCATCAACACCATTCCCTATCACGATAAAATTGCCAACAAGGCCATCTATCTAGGT
    TTGAAATCCATTAAAGGAGTCAGTAATGATTTAGCTCTCTGGATTATTGAAAATAGACCT
    TATTCTAACATTGAAGATTTTATAGCTAAATTACCTGAGAATTATCTGAAACTTCCTCTG
    CTAGAACCTTTGGTAAAAGTTGGTCTTTTCGATTCATTTGAAAAAAATCGTCAAAAAGTA
    TTTAATAACTTAGCTAATCTATTTGAATTTGTGAAAGAGTTGGGAAGTTTGTTTGGAGAT
    GCTATTTATAGTTGGCAGGAATCGGAAGATTGGACGGAACAAGAAAAATTTTATATGGAA
    CAAGAGCTTTTAGGGATAGGTGTCAGCAAACATCCACTACAAGCTATTGCAAGTAAGGCT
    ATTTACCCGATTACCCCAATCGGAAATTTGTCAGAAAATAGCTATGCTATTATCTTGGTT
    GAAGTTCAGAAAATAAAAGTGATTCGTACCAAAAAGGGTGAAAATATGGCCTTCTTACAG
    GCAGATGATAGTAAGAAAAAATTGGATGTCACTCTCTTTTCAGACTTATATCGTCAGGTT
    GGACAGGAAATAAAAGAGGGAGCCTTCTACTATGTAAAAGGAAAAATACAATCACGTGAT
    GGCCGTCTGCAAATGATTGCACAAGAAATAAGAGAAGCAGTTGCTGAACGCTTTTGGATA
    CAGGTGAAAAATCATGAATCGGATCAAGAAATTTCACGCATTTTAGAACAATTTAAAGGC
    CCAATCCCAGTCATCATCCGGTATGAAGAGGAACAGAAAACCATCGTTTCTCCCCATCAT
    TTTGTAGCTAAATCCAATGAATTAGAGGAGAAATTGAATGAAATCGTTATGAAAACGATT
    TATCGCTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1320
    1380
    1440
    1500
    1560
    1620
    1680
    1740
    1800
    1860
    1920
    1980
    2040
    2100
    2160
    2220
    2280
    2340
    2400
    2460
    2520
    2580
    2640
    2700
    2760
    2820
    2880
    2940
    3000
    3060
    3120
    3129

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SP_0895 [new locus tag: SP_RS04415 ]
  • symbol: DnaE
  • description: DNA polymerase III, alpha subunit
  • length: 1042
  • theoretical pI: 5.31586
  • theoretical MW: 119123
  • GRAVY: -0.219002

⊟Function[edit | edit source]

  • reaction:
    EC 2.7.7.7?  ExPASy
    DNA-directed DNA polymerase Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1)
  • TIGRFAM:
    Genetic information processing DNA metabolism DNA replication, recombination, and repair DNA polymerase III, alpha subunit (TIGR00594; EC 2.7.7.7; HMM-score: 757.2)
    and 2 more
    Genetic information processing DNA metabolism DNA replication, recombination, and repair DNA polymerase III, alpha subunit, Gram-positive type (TIGR01405; EC 2.7.7.7; HMM-score: 82)
    Genetic information processing DNA metabolism Degradation of DNA exodeoxyribonuclease VII, large subunit (TIGR00237; EC 3.1.11.6; HMM-score: 13.3)
  • TheSEED  :
    • DNA polymerase III alpha subunit (EC 2.7.7.7)
    DNA Metabolism DNA replication DNA-replication  DNA polymerase III alpha subunit (EC 2.7.7.7)
  • PFAM:
    NTP_transf (CL0260) DNA_pol3_alpha; Bacterial DNA polymerase III alpha NTPase domain (PF07733; HMM-score: 297.3)
    and 7 more
    no clan defined DNA_pol3_finger; Bacterial DNA polymerase III alpha subunit finger domain (PF17657; HMM-score: 197.2)
    Amidohydrolase (CL0034) PHP; PHP domain (PF02811; HMM-score: 65.4)
    HHH (CL0198) HHH_6; Helix-hairpin-helix motif (PF14579; HMM-score: 45.1)
    OB (CL0021) tRNA_anti-codon; OB-fold nucleic acid binding domain (PF01336; HMM-score: 36.3)
    tRNA_anti_2; OB-fold nucleic acid binding domain (PF13742; HMM-score: 14.7)
    no clan defined KID; KID repeat (PF02524; HMM-score: 12.6)
    OB (CL0021) SSB; Single-strand binding protein family (PF00436; HMM-score: 12.5)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 9.97
    • Cytoplasmic Membrane Score: 0
    • Cellwall Score: 0.01
    • Extracellular Score: 0.02
    • Internal Helices: 0
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.007815
    • TAT(Tat/SPI): 0.000327
    • LIPO(Sec/SPII): 0.000449
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MRRIEEKCKLIAQLDTKTVYSFMESVISIEKYVRAAKEYGYTHLAMMDIDNLYGAFDFLEITKKYGIHPLLGLEMTVFVDDQGVNLRFLALSSVGYQQLMKLSTAKMQGEKTWSVLSQYLEDIAVIVPYFDRVESLELGCDYYIGVYPETLASEFHHPILPLYRVNAFESRDREVLQVLTAIKENLPLREVPLRSRQDVFISASSLEKLFQERFPQALDNLEKLISGISYDLDTSLKLPRFNPARPAVEELRERAELGLVQKGLTSKEYQDRLDQELSVIHDMGFDDYFLVVWDLLRFGQSNGYYMGMGRGSAVGSLVSYALDITGIDPVEKNLIFERFLNRERYTMPDIDIDIPDIYRPDFIRYVGNKYGSKHAAQIVTFSTFGAKQALRDVLKRFGVPEYELSAITKKISFRDNLKSAYEGNLQFRQQINSKLEYQKAFEIACKIEGYPRQTSVHAAGVVISDQDLTNYIPLKYGDEIPLTQYDAHGVEASGLLKMDFLGLRNLTFVQKMQELLAETEGIHLKIEEIDLEDKETLALFASGNTKGIFQFEQPGAIRLLKRVQPVCFEDVVATTSLNRPGASDYINNFVARKHGQEEVTVLDPVLEDILAPTYGIMLYQEQVMQVAQRLAGFSLGKADILRRAMGKKDASAMHEMRASFIQGSLEAGHTVEKAEQVFDVMEKFAGYGFNRSHAYAYSALAFQLAYFKTHYPAIFYQVMLNSSNSDYLIDALEAGFEVASLSINTIPYHDKIANKAIYLGLKSIKGVSNDLALWIIENRPYSNIEDFIAKLPENYLKLPLLEPLVKVGLFDSFEKNRQKVFNNLANLFEFVKELGSLFGDAIYSWQESEDWTEQEKFYMEQELLGIGVSKHPLQAIASKAIYPITPIGNLSENSYAIILVEVQKIKVIRTKKGENMAFLQADDSKKKLDVTLFSDLYRQVGQEIKEGAFYYVKGKIQSRDGRLQMIAQEIREAVAERFWIQVKNHESDQEISRILEQFKGPIPVIIRYEEEQKTIVSPHHFVAKSNELEEKLNEIVMKTIYR

⊟Experimental data[edit | edit source]

  • protein localization:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator:

⊟Expression data[edit | edit source]

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ Tim van Opijnen, Andrew Camilli
    A fine scale phenotype-genotype virulence map of a bacterial pathogen.
    Genome Res: 2012, 22(12);2541-51
    [PubMed:22826510] [WorldCat.org] [DOI] (I p)

⊟Relevant publications[edit | edit source]

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