Jump to navigation
Jump to search
PangenomeTIGR4
serotype 4
D39serotype 2
D39Vserotype 2
Hungary19A-6serotype 19A
EF3030serotype 19F
670-6Bserotype 6B
6A-10serotype 6A
70585serotype 5
A026serotype 19F
A66serotype 3
AP200serotype 11A
ASP0581serotype 12F
ATCC 49619serotype 19F
ATCC 700669serotype 23F
BVJ1JLserotype 1
CGSP14serotype 14
G54serotype 19F
HU-OHserotype 3
Hu15serotype 19A
Hu17serotype 19A
INV104serotype 1
INV200serotype 14
JJAserotype 14
MDRSPN001serotype 19F
NCTC7466serotype 2
NU83127serotype 4
OXC141serotype 3
P1031serotype 1
R6serotype 2
SP49serotype 19A
SPN032672serotype 1
SPN034156serotype 3
SPN034183serotype 3
SPN994038serotype 3
SPN994039serotype 3
SPNA45serotype 3
ST556serotype 19F
TCH8431/19Aserotype 19A
Taiwan19F-14serotype 19F
Xen35serotype 4
gamPNI0373serotype 1
NCBI: 31-JAN-2014
⊟Summary[edit | edit source]
- organism: Streptococcus pneumoniae TIGR4
- locus tag: SP_1269 [new locus tag: SP_RS06220 ]
- pan locus tag?: PNEUPAN002555000
- symbol: pck
- pan gene symbol?: licA
- synonym:
- product: choline kinase
⊟Genome View[edit | edit source]
⊟Gene[edit | edit source]
⊟General[edit | edit source]
- type: CDS
- locus tag: SP_1269 [new locus tag: SP_RS06220 ]
- symbol: pck
- product: choline kinase
- replicon: chromosome
- strand: -
- coordinates: 1201797..1202585
- length: 789
- essential: yes [1]
⊟Accession numbers[edit | edit source]
- Location: AE005672 (1201797..1202585) NCBI
- BioCyc: see SP_RS06220
- MicrobesOnline: 116732 MicrobesOnline
- PneumoBrowse for strain D39V: SPV_1125 PneumoBrowse
⊟Phenotype[edit | edit source]
Share your knowledge and add information here. [edit]
⊟DNA sequence[edit | edit source]
- 1
61
121
181
241
301
361
421
481
541
601
661
721
781ATGACCAATCAAAACTATTTGGCCAAAACAACAAATAAGCAATACATTGTTAAATTCTTT
GGTAAAGGGACAGAAAAGCTTATCAATCGACAAGATGAAAAGTACAATCTTGAACTACTA
AAGGATTTAGGCTTAGATGTAAAAAATTATCTTTTTGATATTGAAGCTGGTATCAAAGTA
AATGAGTATATCGAATCTGCGATTACGCTTGATTCAACGTCAATCAAGACCAAGTTCGAC
AAAATTACTCCAATATTACAAACTATTCATACGTCTGCTAAGGAATTAAGAGGAGAATTT
GCTCCTTTTGAAGAAATCAAAAAATACGAATCCTTGATTGAAGAACAAATTCCTTATGCC
AACTATGAATCTGTTAGAAATGCAGTCTTCTCCTTAGAGAAAAGACTGGCTGACTTAGGT
GTTGACAGAAAATCTTGTCATATCGATTTGGTGCCTGAAAACTTTATCGAATCACCTCAA
GGACGACTTTATTTGATTGACTGGGAATATTCATCAATGAATGATCCAATGTGGGATTTG
GCTGCCCTCTTTTTAGAGTCTGAATTCACTTCCCAAGAGGAAGAAACTTTCTTATCTCAC
TATGAGAGTGACCAAACACCGGTTTCTCATGAAAAGATTGCTATTTATAAAATTTTACAA
GATACTATTTGGAGTCTATGGACTGTCTATAAGGAAGAGCAAGGTGAAGATTTTGGTGAC
TATGGTGTGAATCGTTACCAAAGAGCTATTAAAGGTTTGGCTTCTTATGGAGGTTCAGAT
GAAAAGTAA60
120
180
240
300
360
420
480
540
600
660
720
780
789
⊟Protein[edit | edit source]
⊟General[edit | edit source]
- locus tag: SP_1269 [new locus tag: SP_RS06220 ]
- symbol: Pck
- description: choline kinase
- length: 262
- theoretical pI: 4.48734
- theoretical MW: 30446.9
- GRAVY: -0.55687
⊟Function[edit | edit source]
- TIGRFAM: Biosynthesis of cofactors, prosthetic groups, and carriers Thiamine thiamine kinase (TIGR02721; EC 2.7.1.89; HMM-score: 62.2)and 3 morefibrobacter succinogenes paralogous family TIGR02172 (TIGR02172; HMM-score: 23.8)Amino acid biosynthesis Aspartate family homoserine kinase (TIGR00938; EC 2.7.1.39; HMM-score: 18)Cellular processes Sporulation and germination spore coat protein YsxE (TIGR02904; HMM-score: 16.1)
- TheSEED :
- Choline kinase (EC 2.7.1.32)
- PFAM: PKinase (CL0016) Choline_kinase; Choline/ethanolamine kinase (PF01633; HMM-score: 124.5)and 3 moreAPH; Phosphotransferase enzyme family (PF01636; HMM-score: 43.5)DUF1679; Uncharacterized oxidoreductase dhs-27 (PF07914; HMM-score: 13.7)HipA_C; HipA-like C-terminal domain (PF07804; HMM-score: 11.6)
⊟Structure, modifications & cofactors[edit | edit source]
- domains:
- modifications:
- cofactors:
- effectors:
⊟Localization[edit | edit source]
- PSORTb: Cytoplasmic
- Cytoplasmic Score: 7.5
- Cytoplasmic Membrane Score: 1.15
- Cellwall Score: 0.62
- Extracellular Score: 0.73
- Internal Helices: 0
- SignalP: no predicted signal peptide
- SP(Sec/SPI): 0.002964
- TAT(Tat/SPI): 0.000116
- LIPO(Sec/SPII): 0.000353
- predicted transmembrane helices (TMHMM): 0
⊟Accession numbers[edit | edit source]
⊟Protein sequence[edit | edit source]
- MTNQNYLAKTTNKQYIVKFFGKGTEKLINRQDEKYNLELLKDLGLDVKNYLFDIEAGIKVNEYIESAITLDSTSIKTKFDKITPILQTIHTSAKELRGEFAPFEEIKKYESLIEEQIPYANYESVRNAVFSLEKRLADLGVDRKSCHIDLVPENFIESPQGRLYLIDWEYSSMNDPMWDLAALFLESEFTSQEEETFLSHYESDQTPVSHEKIAIYKILQDTIWSLWTVYKEEQGEDFGDYGVNRYQRAIKGLASYGGSDEK
⊟Experimental data[edit | edit source]
- protein localization:
- interaction partners:
⊟Expression & Regulation[edit | edit source]
⊟Operon[edit | edit source]
⊟Regulation[edit | edit source]
- regulator:
⊟Expression data[edit | edit source]
- PneumoExpress for strain D39V: SPV_1125 PneumoExpress
⊟Biological Material[edit | edit source]
⊟Mutants[edit | edit source]
⊟Expression vector[edit | edit source]
⊟lacZ fusion[edit | edit source]
⊟GFP fusion[edit | edit source]
⊟two-hybrid system[edit | edit source]
⊟FLAG-tag construct[edit | edit source]
⊟Antibody[edit | edit source]
⊟Other Information[edit | edit source]
You are kindly invited to share additional interesting facts.
⊟Literature[edit | edit source]
⊟References[edit | edit source]
- ↑ Tim van Opijnen, Andrew Camilli
A fine scale phenotype-genotype virulence map of a bacterial pathogen.
Genome Res: 2012, 22(12);2541-51
[PubMed:22826510] [WorldCat.org] [DOI] (I p)
⊟Relevant publications[edit | edit source]
Lei Wang, Yong-Liang Jiang, Jing-Ren Zhang, Cong-Zhao Zhou, Yuxing Chen
Structural and enzymatic characterization of the choline kinase LicA from Streptococcus pneumoniae.
PLoS One: 2015, 10(3);e0120467
[PubMed:25781969] [WorldCat.org] [DOI] (I e)