From PneumoWiki
Jump to navigation Jump to search
PangenomeTIGR4
serotype 4
D39
serotype 2
D39V
serotype 2
Hungary19A-6
serotype 19A
EF3030
serotype 19F
670-6B
serotype 6B
6A-10
serotype 6A
70585
serotype 5
A026
serotype 19F
A66
serotype 3
AP200
serotype 11A
ASP0581
serotype 12F
ATCC 49619
serotype 19F
ATCC 700669
serotype 23F
BVJ1JL
serotype 1
CGSP14
serotype 14
G54
serotype 19F
HU-OH
serotype 3
Hu15
serotype 19A
Hu17
serotype 19A
INV104
serotype 1
INV200
serotype 14
JJA
serotype 14
MDRSPN001
serotype 19F
NCTC7466
serotype 2
NU83127
serotype 4
OXC141
serotype 3
P1031
serotype 1
R6
serotype 2
SP49
serotype 19A
SPN032672
serotype 1
SPN034156
serotype 3
SPN034183
serotype 3
SPN994038
serotype 3
SPN994039
serotype 3
SPNA45
serotype 3
ST556
serotype 19F
TCH8431/19A
serotype 19A
Taiwan19F-14
serotype 19F
Xen35
serotype 4
gamPNI0373
serotype 1

NCBI: 31-JAN-2014

⊟Summary[edit | edit source]

  • organism: Streptococcus pneumoniae TIGR4
  • locus tag: SP_1329 [new locus tag: SP_RS06525 ]
  • pan locus tag?: PNEUPAN002649000
  • symbol: SP_1329
  • pan gene symbol?: nanA_1
  • synonym:
  • product: N-acetylneuraminate lyase

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SP_1329 [new locus tag: SP_RS06525 ]
  • symbol: SP_1329
  • product: N-acetylneuraminate lyase
  • replicon: chromosome
  • strand: -
  • coordinates: 1253679..1254596
  • length: 918
  • essential: unknown

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    ATGAAAGATTTAACTAAATACAAAGGCGTTATCCCTGCATTTTATGCTTGCTATGATGAA
    AATGGTGAAATTAGCCAAGATCGTGTAAAATCTCTGGTACAATATTTCATTGACAAAGGT
    GTAAAAGGTATCTATGTAAATGGTTCTTCAGGTGAATGTATTTACCAAAGTGTAGAAGAT
    CGTAAACAAATTATTGAAGCTGTTATGGAAGTTGCTAAAGGTAAATTAACAGTTATCAAC
    CATATTGCATGTAATAACACGAAAGATAGTATCGAATTGGCAAAACATTCAGAAAGTGTT
    GGAGTCGATGCTATTGCAGCTATCCCACCTATTTATTTCAAATTGCCAGAGTATTCAATC
    GCAGCATATTGGAATGCAATGAGTGAAGCTGCGTCAAATACAGATTTTATTATCTATAAT
    ATTCCACAATTGGCAGGGGTTGCGTTGACTGGTAGTTTGTATGCAACAATGCGTCAAAAT
    CCTCGTGTGATTGGAGTTAAAAATTCTTCTATGCCTGTACAAGATATTCAAATGTTTGTA
    GCTGCAGGTGGAGAAGATTACATTGTATTCAATGGTCCAGATGAACAATATCTTGGTGGT
    CGCTTGATGGGAGCAGAAGCTGGTATTGGTGGTACTTATGGCGTTATGCCAGATTTGTTC
    TTGAAATTGGAAAGTTTGATTCAAGAACGAGATTTAGATACAGCTAAAAAACTTCAATAT
    GCTATCAATGAAGTTATCTATAAGATGATATCAGGTAAGGCAAATATGTATGCTGTAGCA
    AAAGAAGTTTTGCGTCTAAATGAAAAACTTGATTTAGGTTCTGTTCGTCAACCTTTAGAA
    GCATTGGCAGAAGGTGACTTGGAAGTTGCAAAACAAGCAGCAGAACTTATTCAACAAGCA
    CGAAAAGAATTTTTATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    918

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SP_1329 [new locus tag: SP_RS06525 ]
  • symbol: SP_1329
  • description: N-acetylneuraminate lyase
  • length: 305
  • theoretical pI: 4.76818
  • theoretical MW: 33562.3
  • GRAVY: -0.0767213

⊟Function[edit | edit source]

  • reaction:
    EC 4.1.3.3?  ExPASy
    N-acetylneuraminate lyase N-acetylneuraminate = N-acetyl-D-mannosamine + pyruvate
  • TIGRFAM:
    Cell structure Cell envelope Biosynthesis and degradation of surface polysaccharides and lipopolysaccharides N-acetylneuraminate lyase (TIGR00683; EC 4.1.3.3; HMM-score: 191.7)
    Metabolism Central intermediary metabolism Amino sugars N-acetylneuraminate lyase (TIGR00683; EC 4.1.3.3; HMM-score: 191.7)
    and 3 more
    Metabolism Amino acid biosynthesis Aspartate family 4-hydroxy-tetrahydrodipicolinate synthase (TIGR00674; EC 4.3.3.7; HMM-score: 128.1)
    2,4-dihydroxyhept-2-ene-1,7-dioic acid aldolase (TIGR02313; EC 4.1.2.-; HMM-score: 111.4)
    5-dehydro-4-deoxyglucarate dehydratase (TIGR03249; EC 4.2.1.41; HMM-score: 55.6)
  • TheSEED  :
    • N-acetylneuraminate lyase (EC 4.1.3.3)
    Cell Wall and Capsule Capsular and extracellular polysacchrides Sialic Acid Metabolism  N-acetylneuraminate lyase (EC 4.1.3.3)
  • PFAM:
    TIM_barrel (CL0036) DHDPS; Dihydrodipicolinate synthetase family (PF00701; HMM-score: 182.4)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 9.97
    • Cytoplasmic Membrane Score: 0
    • Cellwall Score: 0.01
    • Extracellular Score: 0.02
    • Internal Helices: 0
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.018279
    • TAT(Tat/SPI): 0.000417
    • LIPO(Sec/SPII): 0.040759
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MKDLTKYKGVIPAFYACYDENGEISQDRVKSLVQYFIDKGVKGIYVNGSSGECIYQSVEDRKQIIEAVMEVAKGKLTVINHIACNNTKDSIELAKHSESVGVDAIAAIPPIYFKLPEYSIAAYWNAMSEAASNTDFIIYNIPQLAGVALTGSLYATMRQNPRVIGVKNSSMPVQDIQMFVAAGGEDYIVFNGPDEQYLGGRLMGAEAGIGGTYGVMPDLFLKLESLIQERDLDTAKKLQYAINEVIYKMISGKANMYAVAKEVLRLNEKLDLGSVRQPLEALAEGDLEVAKQAAELIQQARKEFL

⊟Experimental data[edit | edit source]

  • protein localization:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulators: RpiR* (repression) regulon, NanR* (repression) regulon, CcpA regulon
    RpiR*(TF)important in Sialic acid utilization; RegPrecise 
    NanR*(TF)important in Sialic acid utilization; RegPrecise 
    CcpA(TF)important in Global catabolite repression; RegPrecise 

⊟Expression data[edit | edit source]

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

⊟Relevant publications[edit | edit source]

Retrieved from "http://pneumowiki.med.uni-greifswald.de/index.php?title=SP_1329&oldid=3550"