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PangenomeTIGR4
serotype 4
D39
serotype 2
D39V
serotype 2
Hungary19A-6
serotype 19A
EF3030
serotype 19F
670-6B
serotype 6B
6A-10
serotype 6A
70585
serotype 5
A026
serotype 19F
A66
serotype 3
AP200
serotype 11A
ASP0581
serotype 12F
ATCC 49619
serotype 19F
ATCC 700669
serotype 23F
BVJ1JL
serotype 1
CGSP14
serotype 14
G54
serotype 19F
HU-OH
serotype 3
Hu15
serotype 19A
Hu17
serotype 19A
INV104
serotype 1
INV200
serotype 14
JJA
serotype 14
MDRSPN001
serotype 19F
NCTC7466
serotype 2
NU83127
serotype 4
OXC141
serotype 3
P1031
serotype 1
R6
serotype 2
SP49
serotype 19A
SPN032672
serotype 1
SPN034156
serotype 3
SPN034183
serotype 3
SPN994038
serotype 3
SPN994039
serotype 3
SPNA45
serotype 3
ST556
serotype 19F
TCH8431/19A
serotype 19A
Taiwan19F-14
serotype 19F
Xen35
serotype 4
gamPNI0373
serotype 1

NCBI: 31-JAN-2014

⊟Summary[edit | edit source]

  • organism: Streptococcus pneumoniae TIGR4
  • locus tag: SP_2235 [new locus tag: SP_RS11425 ]
  • pan locus tag?: PNEUPAN003935000
  • symbol: comE
  • pan gene symbol?: comE
  • synonym:
  • product: response regulator ComE

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SP_2235 [new locus tag: SP_RS11425 ]
  • symbol: comE
  • product: response regulator ComE
  • replicon: chromosome
  • strand: -
  • coordinates: 2155665..2156417
  • length: 753
  • essential: unknown

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    ATGAAAGTTTTAATTTTAGAAGATGTTATTGAACATCAAGTGAGACTAGAGAGAATATTG
    GATGAAATTTCGAAAGAATCGAATATTCCAATATCATACAAGACAACGGGAAAAGTCCGT
    GAATTTGAAGAATACATTGAAAATGATGAAGTAAATCAGCTTTATTTCCTAGATATCGAT
    ATTCATGGAATTGAGAAAAAGGGATTTGAAGTGGCTCAGCTCATTCGTCATTACAATCCT
    TACGCTATTATCGTCTTTATCACTAGTCGATCAGAGTTTGCGACTCTAACCTATAAATAC
    CAGGTATCAGCCCTAGATTTTGTTGATAAGGATATCAATGATGAGATGTTTAAGAAGAGA
    ATTGAGCAAAATATCTTCTACACGAAGAGTATGTTACTTGAAAATGAAGATGTTGTAGAT
    TATTTCGACTACAATTACAAGGGAAATGATTTAAAAATTCCTTACCATGATATTTTGTAT
    ATTGAAACAACAGGGGTATCTCATAAATTGCGCATTATTGGTAAGAATTTTGCAAAAGAG
    TTTTATGGTACCATGACAGATATTCAGGAAAAGGACAAACATACTCAGCGATTTTATTCT
    CCTCACAAGTCATTTTTGGTAAATATAGGTAATATCAGAGAAATTGATCGAAAAAACTTA
    GAAATTGTTTTCTATGAAGACCATCGTTGTCCTATTTCAAGATTAAAAATTAGAAAATTA
    AAAGATATTTTAGAGAAAAAATCTCAAAAGTGA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    753

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SP_2235 [new locus tag: SP_RS11425 ]
  • symbol: ComE
  • description: response regulator ComE
  • length: 250
  • theoretical pI: 6.52425
  • theoretical MW: 29958.1
  • GRAVY: -0.538

⊟Function[edit | edit source]

  • TIGRFAM:
    proteobacterial dedicated sortase system response regulator (TIGR03787; HMM-score: 18.6)
  • TheSEED  :
    • Response regulator of the competence regulon ComE
    DNA Metabolism DNA uptake, competence Competence in Streptococci  Response regulator of the competence regulon ComE
  • PFAM:
    Tudor (CL0049) LytTR; LytTr DNA-binding domain (PF04397; HMM-score: 79.7)
    and 3 more
    CheY (CL0304) Response_reg; Response regulator receiver domain (PF00072; HMM-score: 36.2)
    cREC_REC; Cyclic-phosphate processing Receiver domain (PF20274; HMM-score: 17.7)
    no clan defined RRM_4; RNA recognition motif of the spliceosomal PrP8 (PF10598; HMM-score: 13.8)

⊟Structure, modifications & cofactors[edit | edit source]

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 9.97
    • Cytoplasmic Membrane Score: 0
    • Cellwall Score: 0.01
    • Extracellular Score: 0.02
    • Internal Helices: 0
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.003417
    • TAT(Tat/SPI): 0.000168
    • LIPO(Sec/SPII): 0.000423
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MKVLILEDVIEHQVRLERILDEISKESNIPISYKTTGKVREFEEYIENDEVNQLYFLDIDIHGIEKKGFEVAQLIRHYNPYAIIVFITSRSEFATLTYKYQVSALDFVDKDINDEMFKKRIEQNIFYTKSMLLENEDVVDYFDYNYKGNDLKIPYHDILYIETTGVSHKLRIIGKNFAKEFYGTMTDIQEKDKHTQRFYSPHKSFLVNIGNIREIDRKNLEIVFYEDHRCPISRLKIRKLKDILEKKSQK

⊟Experimental data[edit | edit source]

  • protein localization:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator: ComE (activation) regulon
    ComE(response regulator)activating expression of early competence genes [1]

⊟Expression data[edit | edit source]

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ 1.0 1.1 Jelle Slager, Rieza Aprianto, Jan-Willem Veening
    Refining the Pneumococcal Competence Regulon by RNA Sequencing.
    J Bacteriol: 2019, 201(13);
    [PubMed:30885934] [WorldCat.org] [DOI] (I e)

⊟Relevant publications[edit | edit source]

O Ween, P Gaustad, L S Håvarstein
Identification of DNA binding sites for ComE, a key regulator of natural competence in Streptococcus pneumoniae.
Mol Microbiol: 1999, 33(4);817-27
[PubMed:10447890] [WorldCat.org] [DOI] (P p)Bernard Martin, Chantal Granadel, Nathalie Campo, Vincent Hénard, Marc Prudhomme, Jean-Pierre Claverys
Expression and maintenance of ComD-ComE, the two-component signal-transduction system that controls competence of Streptococcus pneumoniae.
Mol Microbiol: 2010, 75(6);1513-28
[PubMed:20180906] [WorldCat.org] [DOI] (I p)Claudia Trappetti, Luciana Gualdi, Lorenzo Di Meola, Prashant Jain, Cindy C Korir, Paul Edmonds, Francesco Iannelli, Susanna Ricci, Gianni Pozzi, Marco R Oggioni
The impact of the competence quorum sensing system on Streptococcus pneumoniae biofilms varies depending on the experimental model.
BMC Microbiol: 2011, 11;75
[PubMed:21492426] [WorldCat.org] [DOI] (I e)M S Chandler, D A Morrison
Competence for genetic transformation in Streptococcus pneumoniae: molecular cloning of com, a competence control locus.
J Bacteriol: 1987, 169(5);2005-11
[PubMed:3032904] [WorldCat.org] [DOI] (P p)L S Håvarstein, G Coomaraswamy, D A Morrison
An unmodified heptadecapeptide pheromone induces competence for genetic transformation in Streptococcus pneumoniae.
Proc Natl Acad Sci U S A: 1995, 92(24);11140-4
[PubMed:7479953] [WorldCat.org] [DOI] (P p)E V Pestova, L S Håvarstein, D A Morrison
Regulation of competence for genetic transformation in Streptococcus pneumoniae by an auto-induced peptide pheromone and a two-component regulatory system.
Mol Microbiol: 1996, 21(4);853-62
[PubMed:8878046] [WorldCat.org] [DOI] (P p)L S Håvarstein, P Gaustad, I F Nes, D A Morrison
Identification of the streptococcal competence-pheromone receptor.
Mol Microbiol: 1996, 21(4);863-9
[PubMed:8878047] [WorldCat.org] [DOI] (P p)

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