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PangenomeTIGR4
serotype 4
D39
serotype 2
D39V
serotype 2
Hungary19A-6
serotype 19A
EF3030
serotype 19F
670-6B
serotype 6B
6A-10
serotype 6A
70585
serotype 5
A026
serotype 19F
A66
serotype 3
AP200
serotype 11A
ASP0581
serotype 12F
ATCC 49619
serotype 19F
ATCC 700669
serotype 23F
BVJ1JL
serotype 1
CGSP14
serotype 14
G54
serotype 19F
HU-OH
serotype 3
Hu15
serotype 19A
Hu17
serotype 19A
INV104
serotype 1
INV200
serotype 14
JJA
serotype 14
MDRSPN001
serotype 19F
NCTC7466
serotype 2
NU83127
serotype 4
OXC141
serotype 3
P1031
serotype 1
R6
serotype 2
SP49
serotype 19A
SPN032672
serotype 1
SPN034156
serotype 3
SPN034183
serotype 3
SPN994038
serotype 3
SPN994039
serotype 3
SPNA45
serotype 3
ST556
serotype 19F
TCH8431/19A
serotype 19A
Taiwan19F-14
serotype 19F
Xen35
serotype 4
gamPNI0373
serotype 1

NCBI: 07-FEB-2021

⊟Summary[edit | edit source]

  • organism: Streptococcus pneumoniae AP200
  • locus tag: SPAP_RS08575 [old locus tag: SPAP_1730 ]
  • pan locus tag?: PNEUPAN003133000
  • symbol: SPAP_RS08575
  • pan gene symbol?: scrR
  • synonym:
  • product: LacI family DNA-binding transcriptional regulator

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SPAP_RS08575 [old locus tag: SPAP_1730 ]
  • symbol: SPAP_RS08575
  • product: LacI family DNA-binding transcriptional regulator
  • replicon: chromosome
  • strand: +
  • coordinates: 1636929..1637894
  • length: 966
  • essential: unknown

⊟Accession numbers[edit | edit source]

  • Location: NC_014494 (1636929..1637894) NCBI
  • BioCyc: SPAP_RS08575 BioCyc
  • MicrobesOnline:
  • PneumoBrowse for strain D39V: SPV_1535 PneumoBrowse

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    ATGGTCGCAAAACTAACTGATGTCGCCAAACTTGCAGGCGTCAGTCCTACTACCGTTTCT
    CGGGTTATCAATAAAAAAGGGTATCTATCTGAGAAAACCATCCAAAAAGTCAATGAAGCC
    ATGAGAGAATTGGGCTATAAACCCAACAACCTGGCTCGTAGTCTGCAAGGAAAATCAGCT
    AAGTTAATCGGCTTGATTTTCCCCAATATTTCCAATGTTTTCTATGCAGAATTGATTGAT
    AAATTGGAACACCAACTCTTCAAAAATGGTTACAAGACCATCATCTGCAACAGTGAACAT
    GATTCTGAGAAGGAACGCGAATACATCGAAATGTTGGAAGCCAATCAGGTAGACGGCATC
    ATTTCTGGTAGTCACAACCTAGGAATCGAAGACTACAATCGTGTGACAGCGCCGATTATT
    TCCTTTGACCGAAACCTATCGCCAGACATCCCTGTTGTCTCCTCTGACAACTATGCTGGT
    GGGGTTCTTGCTGCCCAAACCTTGGTCAAGACAGGTGCCCAGTCTATCATCATGATTACA
    GGGAATGACAATTCCAATTCGCCAACCGGACTGCGCCACGCTGGTTTTGCATCCGTACTC
    CCAAAAGCTCCTATTATCAATGTTTCCAGTGACTTTTCTCCCGTCAGAAAAGAAATGGAA
    ATCAAGAATATCTTGACCCGGGAAAAACCAGATGCCATTTTTGCTTCGGATGATTTGACA
    GCTATTCTGGTCATTAAAATCGCTCAAGAATTGGGCATTTCTGTCCCAAAAGAGCTCAAG
    GTCATCGGCTATGATGGGACCTACTTTATCGAAAATTACTACCCTCAATTGGCTACTATC
    AAGCAACCTTTGGAAGAGATTGCTTGTCTCACTATTGATCTTCTCTTGCAAAAGATTGAA
    GGCAAGGAAGTCGCCACAACTGGTTACTTCTTACCAGTTACGCTATTACCAGGAAAAAGT
    ATTTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    966

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SPAP_RS08575 [old locus tag: SPAP_1730 ]
  • symbol: SPAP_RS08575
  • description: LacI family DNA-binding transcriptional regulator
  • length: 321
  • theoretical pI: 6.16193
  • theoretical MW: 35184.3
  • GRAVY: -0.0601246

⊟Function[edit | edit source]

  • ⊞TIGRFAM:
    Signal transduction Regulatory functions DNA interactions catabolite control protein A (TIGR01481; HMM-score: 172.8)
    Signal transduction Regulatory functions DNA interactions D-fructose-responsive transcription factor (TIGR02417; HMM-score: 146.4)
    and 4 more
    Signal transduction Regulatory functions DNA interactions trehalose operon repressor (TIGR02405; HMM-score: 101.2)
    Genetic information processing Mobile and extrachromosomal element functions Other addiction module antidote protein, HigA family (TIGR02607; HMM-score: 12.9)
    Signal transduction Regulatory functions DNA interactions addiction module antidote protein, HigA family (TIGR02607; HMM-score: 12.9)
    Signal transduction Regulatory functions Protein interactions addiction module antidote protein, HigA family (TIGR02607; HMM-score: 12.9)
  • TheSEED: see SPAP_1730
  • ⊞PFAM:
    Periplas_BP (CL0144) Peripla_BP_3; Periplasmic binding protein-like domain (PF13377; HMM-score: 85.8)
    HTH (CL0123) LacI; Bacterial regulatory proteins, lacI family (PF00356; HMM-score: 73.3)
    Periplas_BP (CL0144) Peripla_BP_4; Periplasmic binding protein domain (PF13407; HMM-score: 70.4)
    and 13 more
    Peripla_BP_1; Periplasmic binding proteins and sugar binding domain of LacI family (PF00532; HMM-score: 64.1)
    HTH (CL0123) HTH_3; Helix-turn-helix (PF01381; HMM-score: 25.6)
    HTH_23; Homeodomain-like domain (PF13384; HMM-score: 15.5)
    HTH_Tnp_ISL3; Helix-turn-helix domain of transposase family ISL3 (PF13542; HMM-score: 15.4)
    HTH_10; HTH DNA binding domain (PF04967; HMM-score: 13.5)
    HTH_26; Cro/C1-type HTH DNA-binding domain (PF13443; HMM-score: 13.4)
    HTH_24; Winged helix-turn-helix DNA-binding (PF13412; HMM-score: 13.3)
    Fe_dep_repress; Iron dependent repressor, N-terminal DNA binding domain (PF01325; HMM-score: 13.2)
    HTH_38; Helix-turn-helix domain (PF13936; HMM-score: 13)
    HTH_17; Helix-turn-helix domain (PF12728; HMM-score: 12.9)
    Sigma54_DBD; Sigma-54, DNA binding domain (PF04552; HMM-score: 12.6)
    HTH_31; Helix-turn-helix domain (PF13560; HMM-score: 12.5)
    HTH_7; Helix-turn-helix domain of resolvase (PF02796; HMM-score: 12.3)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:
  • genes regulated by ScrR, TF important in Sucrose utilization: in TIGR4

⊟Localization[edit | edit source]

  • ⊞PSORTb: Cytoplasmic
    • Cytoplasmic Score: 9.97
    • Cytoplasmic Membrane Score: 0
    • Cellwall Score: 0.01
    • Extracellular Score: 0.02
    • Internal Helices: 0
  • ⊞SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.007106
    • TAT(Tat/SPI): 0.001105
    • LIPO(Sec/SPII): 0.001272
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

  • GI:
  • RefSeq: WP_000226001 NCBI
  • UniProt:

⊟Protein sequence[edit | edit source]

  • MVAKLTDVAKLAGVSPTTVSRVINKKGYLSEKTIQKVNEAMRELGYKPNNLARSLQGKSAKLIGLIFPNISNVFYAELIDKLEHQLFKNGYKTIICNSEHDSEKEREYIEMLEANQVDGIISGSHNLGIEDYNRVTAPIISFDRNLSPDIPVVSSDNYAGGVLAAQTLVKTGAQSIIMITGNDNSNSPTGLRHAGFASVLPKAPIINVSSDFSPVRKEMEIKNILTREKPDAIFASDDLTAILVIKIAQELGISVPKELKVIGYDGTYFIENYYPQLATIKQPLEEIACLTIDLLLQKIEGKEVATTGYFLPVTLLPGKSI

⊟Experimental data[edit | edit source]

  • protein localization:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

⊟Expression data[edit | edit source]

⊞Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊞Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

⊟Relevant publications[edit | edit source]

Ramkumar Iyer, Andrew Camilli
Sucrose metabolism contributes to in vivo fitness of Streptococcus pneumoniae.
Mol Microbiol: 2007, 66(1);1-13
[PubMed:17880421] [WorldCat.org] [DOI] (P p)

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