⊟Summary[edit | edit source]

PangenomeTIGR4

serotype 4

D39

serotype 2

D39V

serotype 2

Hungary19A-6

serotype 19A

EF3030

serotype 19F

670-6B

serotype 6B

6A-10

serotype 6A

70585

serotype 5

A026

serotype 19F

A66

serotype 3

AP200

serotype 11A

ASP0581

serotype 12F

ATCC 49619

serotype 19F

ATCC 700669

serotype 23F

BVJ1JL

serotype 1

CGSP14

serotype 14

G54

serotype 19F

HU-OH

serotype 3

Hu15

serotype 19A

Hu17

serotype 19A

INV104

serotype 1

INV200

serotype 14

JJA

serotype 14

MDRSPN001

serotype 19F

NCTC7466

serotype 2

NU83127

serotype 4

OXC141

serotype 3

P1031

serotype 1

R6

serotype 2

SP49

serotype 19A

SPN032672

serotype 1

SPN034156

serotype 3

SPN034183

serotype 3

SPN994038

serotype 3

SPN994039

serotype 3

SPNA45

serotype 3

ST556

serotype 19F

TCH8431/19A

serotype 19A

Taiwan19F-14

serotype 19F

Xen35

serotype 4

gamPNI0373

serotype 1

NCBI: 26-OCT-2021

⊟Summary

• organism: Streptococcus pneumoniae R6
locus tag: SPR_RS07130 [old locus tag: spr1436 ]
pan locus tag^?: PNEUPAN002962000
symbol: SPR_RS07130
pan gene symbol^?: rebM
• synonym:
• product: class I SAM-dependent methyltransferase

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: SPR_RS07130 [old locus tag: spr1436 ]
• symbol: SPR_RS07130
• product: class I SAM-dependent methyltransferase
• replicon: chromosome
• strand: -
• coordinates: 1419322..1420080
• length: 759
• essential: unknown

⊟Accession numbers

• Location: NC_003098 (1419322..1420080) NCBI
• BioCyc: see spr1436
• MicrobesOnline: see spr1436
• PneumoBrowse for strain D39V: SPV_1408 PneumoBrowse

⊟Phenotype

Share your knowledge and add information here. [edit]

⊟DNA sequence

• 1
  61
  121
  181
  241
  301
  361
  421
  481
  541
  601
  661
  721
  
  ATGTCAGAAGCAGGTCATAAGTTTTTAGCAAAATTGGGGAAAAAACGCTTACGTCCAGGT
  GGAAAGCGTGCCACAGATTGGTTAATTGCAGAAGGAGGATTTTCAAAAGAAAAGAGAATA
  CTAGAGGTTGCGTGTAATAGGGGAACTACAGCAATTGAGTTGGCACAGCGTTTTGGTTGC
  AAGATAACTGCTGTTGATATGGATGCTCAAGCTTTAGAAGTGGCTAAAAAATCTGCTGGA
  ACGGCAGGTGTTGCTCATTTAATCAGTTTTGAAAGAGCAAATGCAATGAAACTTCCTTAT
  CAAGATGCTAGTTTTGATATTGTTATAAATGAAGCTATGCTGACTATGCAAGCCGATCAA
  GCTAAGAAAAAATGTGTAATGGAATATCTAAGGGTATTAAAACCTGGAGGTCTTCTCTTG
  ACACATGATGTGCTTCTTAAGGAAGCTAAAGAGTCTATCAGACAGGAATTATCACAAGCA
  ATTCATGTAAATGTAGGTCCTTTAACTCAAGATGGTTGGGAACAGGTGATGATAGAATCA
  GGTTATTGTGATGTGAAAGCATTGACTGGTGAAATGACATTAATGAAATTATCGGGTATG
  ATTTATGACGAAGGTTTGCTAGGAACTTTGAAAATTTGTGTAAATGCTTGTAAAAAGGAG
  AATAGAAAGCAGTTTTTAACTATGTATAAAATGTTTGCTAAGAATAAACAGAAATTGGGC
  TTTATTGCGATGGCTAGTTATAAATCGTCAAAACGTTAG
  60
  120
  180
  240
  300
  360
  420
  480
  540
  600
  660
  720
  759
  

⊟Protein

⊟General

• locus tag: SPR_RS07130 [old locus tag: spr1436 ]
• symbol: SPR_RS07130
• description: class I SAM-dependent methyltransferase
• length: 252
• theoretical pI: 9.96259
• theoretical MW: 27930.7
• GRAVY: -0.19246

⊟Function

• ⊞⊞TIGRFAM:
  Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Menaquinone and ubiquinone ubiquinone/menaquinone biosynthesis methyltransferase (TIGR01934; EC 2.1.1.-; HMM-score: 57.6)
  and 15 more
  Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Menaquinone and ubiquinone demethylmenaquinone methyltransferase (TIGR02752; EC 2.1.1.163; HMM-score: 40.5)
  Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Biotin malonyl-acyl carrier protein O-methyltransferase BioC (TIGR02072; EC 2.1.1.-; HMM-score: 38.3)
  Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Menaquinone and ubiquinone 3-demethylubiquinone-9 3-O-methyltransferase (TIGR01983; EC 2.1.1.64; HMM-score: 37.3)
  Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Glutathione and analogs putative 4-mercaptohistidine N1-methyltranferase (TIGR04345; HMM-score: 33.6)
  methyltransferase, Rta_06860 family (TIGR04290; EC 2.1.1.-; HMM-score: 32.2)
  Genetic information processing Protein fate Protein modification and repair methyltransferase, HemK family (TIGR00536; HMM-score: 27.3)
  Genetic information processing Protein fate Protein modification and repair protein-(glutamine-N5) methyltransferase, release factor-specific (TIGR03534; EC 2.1.1.-; HMM-score: 25.8)
  Genetic information processing Protein synthesis Ribosomal proteins: synthesis and modification ribosomal protein L11 methyltransferase (TIGR00406; EC 2.1.1.-; HMM-score: 25.7)
  Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Chlorophyll and bacteriochlorphyll magnesium protoporphyrin O-methyltransferase (TIGR02021; EC 2.1.1.11; HMM-score: 24.6)
  Genetic information processing Protein synthesis Ribosomal proteins: synthesis and modification protein-(glutamine-N5) methyltransferase, ribosomal protein L3-specific (TIGR03533; EC 2.1.1.-; HMM-score: 22.9)
  Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Heme, porphyrin, and cobalamin precorrin-6Y C5,15-methyltransferase (decarboxylating), CbiT subunit (TIGR02469; EC 2.1.1.132; HMM-score: 18.5)
  2-ketoarginine methyltransferase (TIGR04543; EC 2.1.1.243; HMM-score: 15.4)
  Metabolism Amino acid biosynthesis Aspartate family methionine biosynthesis protein MetW (TIGR02081; HMM-score: 15.1)
  Unknown function Enzymes of unknown specificity putative methylase (TIGR00537; HMM-score: 14.9)
  pseudaminic acid biosynthesis-associated methylase (TIGR03587; HMM-score: 13.1)
• TheSEED: see spr1436
• ⊞⊞PFAM:
  NADP_Rossmann (CL0063) Methyltransf_31; Methyltransferase domain (PF13847; HMM-score: 71.8)
  Methyltransf_25; Methyltransferase domain (PF13649; HMM-score: 71.1)
  Methyltransf_11; Methyltransferase domain (PF08241; HMM-score: 67.2)
  and 19 more
  Ubie_methyltran; ubiE/COQ5 methyltransferase family (PF01209; HMM-score: 50.5)
  Methyltransf_23; Methyltransferase domain (PF13489; HMM-score: 43.5)
  Methyltransf_3; O-methyltransferase (PF01596; HMM-score: 29.2)
  UPF0020; Putative RNA methylase family UPF0020 (PF01170; HMM-score: 29.1)
  Methyltransf_12; Methyltransferase domain (PF08242; HMM-score: 28.9)
  MTS; Methyltransferase small domain (PF05175; HMM-score: 27.3)
  CheR; CheR methyltransferase, SAM binding domain (PF01739; HMM-score: 25.5)
  ADH_zinc_N; Zinc-binding dehydrogenase (PF00107; HMM-score: 23.7)
  CMAS; Mycolic acid cyclopropane synthetase (PF02353; HMM-score: 22.8)
  PrmA; Ribosomal protein L11 methyltransferase (PrmA) (PF06325; HMM-score: 22.1)
  Methyltransf_24; Methyltransferase domain (PF13578; HMM-score: 18.6)
  Methyltransf_15; RNA cap guanine-N2 methyltransferase (PF09445; HMM-score: 16.1)
  Cons_hypoth95; Conserved hypothetical protein 95 (PF03602; HMM-score: 15.6)
  NAS; Nicotianamine synthase protein (PF03059; HMM-score: 15.4)
  Methyltransf_8; Hypothetical methyltransferase (PF05148; HMM-score: 15)
  AlaDh_PNT_C; Alanine dehydrogenase/PNT, C-terminal domain (PF01262; HMM-score: 14.2)
  MetW; Methionine biosynthesis protein MetW (PF07021; HMM-score: 12.6)
  Spermine_synth; Spermine/spermidine synthase domain (PF01564; HMM-score: 12.2)
  no clan defined G6B; G6B family (PF15096; HMM-score: 11.9)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• ⊞⊞PSORTb: Cytoplasmic
  • Cytoplasmic Score: 7.5
  • Cytoplasmic Membrane Score: 1.15
  • Cellwall Score: 0.62
  • Extracellular Score: 0.73
  • Internal Helices: 0
• ⊞⊞SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.008778
  • TAT(Tat/SPI): 0.000737
  • LIPO(Sec/SPII): 0.001379
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI:
• RefSeq: WP_001287230 NCBI
• UniProt: see spr1436

⊟Protein sequence

• MSEAGHKFLAKLGKKRLRPGGKRATDWLIAEGGFSKEKRILEVACNRGTTAIELAQRFGCKITAVDMDAQALEVAKKSAGTAGVAHLISFERANAMKLPYQDASFDIVINEAMLTMQADQAKKKCVMEYLRVLKPGGLLLTHDVLLKEAKESIRQELSQAIHVNVGPLTQDGWEQVMIESGYCDVKALTGEMTLMKLSGMIYDEGLLGTLKICVNACKKENRKQFLTMYKMFAKNKQKLGFIAMASYKSSKR

⊟Experimental data

• protein localization:
• interaction partners:

⊟Expression & Regulation

⊟Operon

• MicrobesOnline: SPR_RS07125 < SPR_RS07130

⊟Regulation

• regulator: CodY see spr1436

⊟Expression data

• PneumoExpress for strain D39V: SPV_1408 PneumoExpress

⊞Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊞Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications