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serotype 3
SPN034183
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SPN994038
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SPN994039
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ST556
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TCH8431/19A
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Taiwan19F-14
serotype 19F
Xen35
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gamPNI0373
serotype 1

NCBI: 26-OCT-2021

⊟Summary[edit | edit source]

  • organism: Streptococcus pneumoniae R6
  • locus tag: SPR_RS08050 [old locus tag: spr1621 ]
  • pan locus tag?: PNEUPAN003280000
  • symbol: SPR_RS08050
  • pan gene symbol?: rbsR
  • synonym:
  • product: LacI family DNA-binding transcriptional regulator

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SPR_RS08050 [old locus tag: spr1621 ]
  • symbol: SPR_RS08050
  • product: LacI family DNA-binding transcriptional regulator
  • replicon: chromosome
  • strand: -
  • coordinates: 1595432..1596427
  • length: 996
  • essential: unknown

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    ATGGTAGAACAAAGAAAATCAATTACCATGAAAGATGTTGCTTTAGAAGCAGGAGTTAGT
    GTTGGAACTGTTTCACGTGTAATTAATAAAGAAAAAGGCATTAAAGAAGTAACTTTAAGG
    AAAGTTCAAAGGGCTATAAAAACATTGGACTATATCCCTGATAATTATGCTCGAGGTATG
    AAAAAGAATAGAACAGAAACTATCGCTTTGATTATTCCGAGCATATGGCATCCATTTTTT
    TCGGAGTTTGCCTATCATGTTGAGAAAGAGTTATTAAAATATAATTATAAATTATTTATT
    TGTAACGCTGATAGCGATTCACAAAATGAAATCGAATATATCGAAATGTTGAAACAAAAT
    AAAGTGGATGGAATTATTGGTATTACATATTCTGATATTGACAAATATATTTTATCTGAT
    TTACCGTTTGTAAGTATAGATAGACATTTTTCGGAAAATGTATGCTATGTTACTTCAGCA
    AATTACAAAGGAGGACAAACTGCAGCAGAGGAACTGGTTAGAAGGGATAATAAGCACCTT
    GCTTTTATTGGTTCAATTAATATGTATGAGAATGAGACTATACTCAGAAGTAAAGGATTC
    AGAGAAAAAGCTAAGGAATTAGGAGTAGATGTCTATAATTTTGAAATGAAAGAACCTTTA
    ATAAATCCTGAAGATCAGATAAGAATTTTTTTGCAATCTCATAAAGAAATTGATGGTATA
    TTTACAATAAATGATATGATGGCTCTGAGAGTTATTAGTGTTATGAAAGAACTGGGGAAA
    ATAGCACCTGATGATTACCAGATAATTGGGTTTGATGGTTTAAAAACTTCTGTCGATCAA
    TCTTATCTAGTGTCTACAATAGTTCAAGATATTGGTATGATGGCAAAAGAATCTGTTGCA
    ATGATACTGAAAATGATTAAGCGAGAAGAGATTCTAGAAAAACGAATCGTAATTCCCACA
    TATTTTTGGGGAGATAAAACAACAAAAAAAATTTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    996

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SPR_RS08050 [old locus tag: spr1621 ]
  • symbol: SPR_RS08050
  • description: LacI family DNA-binding transcriptional regulator
  • length: 331
  • theoretical pI: 7.06281
  • theoretical MW: 38114.9
  • GRAVY: -0.245619

⊟Function[edit | edit source]

  • ⊞TIGRFAM:
    Signal transduction Regulatory functions DNA interactions catabolite control protein A (TIGR01481; HMM-score: 152.7)
    and 6 more
    Signal transduction Regulatory functions DNA interactions D-fructose-responsive transcription factor (TIGR02417; HMM-score: 104.6)
    Signal transduction Regulatory functions DNA interactions trehalose operon repressor (TIGR02405; HMM-score: 61.1)
    Signal transduction Regulatory functions DNA interactions transcriptional repressor BetI (TIGR03384; HMM-score: 14.5)
    Genetic information processing Transcription Transcription factors RNA polymerase sigma-54 factor (TIGR02395; HMM-score: 13.7)
    Signal transduction Regulatory functions DNA interactions transcriptional regulator, y4mF family (TIGR03070; HMM-score: 13.4)
    Metabolism Energy metabolism Photosynthesis chlorophyllide reductase iron protein subunit X (TIGR02016; HMM-score: 11.7)
  • TheSEED: see spr1621
  • ⊞PFAM:
    Periplas_BP (CL0144) Peripla_BP_1; Periplasmic binding proteins and sugar binding domain of LacI family (PF00532; HMM-score: 73)
    Peripla_BP_4; Periplasmic binding protein domain (PF13407; HMM-score: 72)
    HTH (CL0123) LacI; Bacterial regulatory proteins, lacI family (PF00356; HMM-score: 62.6)
    and 11 more
    Periplas_BP (CL0144) Peripla_BP_3; Periplasmic binding protein-like domain (PF13377; HMM-score: 53.5)
    HTH (CL0123) HTH_3; Helix-turn-helix (PF01381; HMM-score: 22.2)
    Periplas_BP (CL0144) Peripla_BP_6; Periplasmic binding protein (PF13458; HMM-score: 18.4)
    HTH (CL0123) HTH_Tnp_ISL3; Helix-turn-helix domain of transposase family ISL3 (PF13542; HMM-score: 16.9)
    HTH_24; Winged helix-turn-helix DNA-binding (PF13412; HMM-score: 14.4)
    TetR_N; Bacterial regulatory proteins, tetR family (PF00440; HMM-score: 14.3)
    PLD (CL0479) PLDc; Phospholipase D Active site motif (PF00614; HMM-score: 13.2)
    HTH (CL0123) HTH_Crp_2; Crp-like helix-turn-helix domain (PF13545; HMM-score: 13)
    no clan defined TnsD; Tn7-like transposition protein D (PF15978; HMM-score: 12.9)
    HTH (CL0123) HTH_AraC; Bacterial regulatory helix-turn-helix proteins, AraC family (PF00165; HMM-score: 12.6)
    no clan defined ATPgrasp_N; ATP-grasp N-terminal domain (PF18130; HMM-score: 12.5)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:
  • genes regulated by RbsR*, TF important in Fructooligosaccharides utilization: see spr1621

⊟Localization[edit | edit source]

  • ⊞PSORTb: Cytoplasmic
    • Cytoplasmic Score: 9.97
    • Cytoplasmic Membrane Score: 0
    • Cellwall Score: 0.01
    • Extracellular Score: 0.02
    • Internal Helices: 0
  • ⊞SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.007088
    • TAT(Tat/SPI): 0.001413
    • LIPO(Sec/SPII): 0.001943
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MVEQRKSITMKDVALEAGVSVGTVSRVINKEKGIKEVTLRKVQRAIKTLDYIPDNYARGMKKNRTETIALIIPSIWHPFFSEFAYHVEKELLKYNYKLFICNADSDSQNEIEYIEMLKQNKVDGIIGITYSDIDKYILSDLPFVSIDRHFSENVCYVTSANYKGGQTAAEELVRRDNKHLAFIGSINMYENETILRSKGFREKAKELGVDVYNFEMKEPLINPEDQIRIFLQSHKEIDGIFTINDMMALRVISVMKELGKIAPDDYQIIGFDGLKTSVDQSYLVSTIVQDIGMMAKESVAMILKMIKREEILEKRIVIPTYFWGDKTTKKI

⊟Experimental data[edit | edit source]

  • protein localization:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator:

⊟Expression data[edit | edit source]

⊞Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊞Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

⊟Relevant publications[edit | edit source]

Ramkumar Iyer, Andrew Camilli
Sucrose metabolism contributes to in vivo fitness of Streptococcus pneumoniae.
Mol Microbiol: 2007, 66(1);1-13
[PubMed:17880421] [WorldCat.org] [DOI] (P p)

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