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From PneumoWiki
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PangenomeTIGR4
serotype 4
D39
serotype 2
D39V
serotype 2
Hungary19A-6
serotype 19A
EF3030
serotype 19F
670-6B
serotype 6B
6A-10
serotype 6A
70585
serotype 5
A026
serotype 19F
A66
serotype 3
AP200
serotype 11A
ASP0581
serotype 12F
ATCC 49619
serotype 19F
ATCC 700669
serotype 23F
BM6001
serotype 19F
BVJ1JL
serotype 1
CGSP14
serotype 14
G54
serotype 19F
HU-OH
serotype 3
Hu15
serotype 19A
Hu17
serotype 19A
INV104
serotype 1
INV200
serotype 14
JJA
serotype 14
MDRSPN001
serotype 19F
NCTC7465
serotype 1
NCTC7466
serotype 2
NU83127
serotype 4
OXC141
serotype 3
P1031
serotype 1
R6
serotype 2
SP49
serotype 19A
SPN032672
serotype 1
SPN034156
serotype 3
SPN034183
serotype 3
SPN994038
serotype 3
SPN994039
serotype 3
SPNA45
serotype 3
ST556
serotype 19F
TCH8431/19A
serotype 19A
Taiwan19F-14
serotype 19F
Xen35
serotype 4
gamPNI0373
serotype 1

NCBI: 25-OCT-2019

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⊟Summary[edit | edit source]

Contents

  • 1 Summary
  • 2 Genome View
  • 3 Gene
    • 3.1 General
    • 3.2 Accession numbers
    • 3.3 Phenotype
    • 3.4 DNA sequence
  • 4 Protein
    • 4.1 General
    • 4.2 Function
    • 4.3 Structure, modifications & cofactors
    • 4.4 Localization
    • 4.5 Accession numbers
    • 4.6 Protein sequence
    • 4.7 Experimental data
  • 5 Expression & Regulation
    • 5.1 Operon
    • 5.2 Regulation
    • 5.3 Expression data
  • 6 Biological Material
    • 6.1 Mutants
    • 6.2 Expression vector
    • 6.3 lacZ fusion
    • 6.4 GFP fusion
    • 6.5 two-hybrid system
    • 6.6 FLAG-tag construct
    • 6.7 Antibody
  • 7 Other Information
  • 8 Literature
    • 8.1 References
    • 8.2 Relevant publications
  • organism: Streptococcus pneumoniae HU-OH
  • locus tag: SPOH_0092 [new locus tag: EL271_RS00540 ]
  • pan locus tag?: PNEUPAN000749000
  • symbol: SPOH_0092
  • pan gene symbol?: gph
  • synonym:
  • product: 5'-nucleotidase

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SPOH_0092 [new locus tag: EL271_RS00540 ]
  • symbol: SPOH_0092
  • product: 5'-nucleotidase
  • replicon: chromosome
  • strand: -
  • coordinates: 98883..99515
  • length: 633
  • essential: unknown

⊟Accession numbers[edit | edit source]

  • Location: AP018937 (98883..99515) NCBI
  • BioCyc: see EL271_RS00540
  • MicrobesOnline:
  • PneumoBrowse for strain D39V: SPV_0101 PneumoBrowse

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    ATGACCTCTATCACAGCGATTTTTTTCGATTTGGATGGAACCCTCGTTGATAGTTCTATC
    GGGATTCACAATGCCTTTACCTATACCTTTAAAGAGCTGGGGGTGCCTAGCCCTGATGCC
    AAAACTATTCGTGGTTTTATGGGACCACCTCTCGAAAGTAGTTTTGCGACCTGCCTGTCC
    AAAGACCAAATTTCTGAAGCCGTGCAGATATATCGTTCTTACTATAAGGCAAAAGGCATC
    TATGAAGCTCAACTCTTTCCTCAGATTATAGACTTGCTTGAGGAGTTATCGAGCAGTTAT
    CCACTCTACATCACCACGACAAAGAATACATCTACTGCTCAAGACATGGCAAAAAACTTG
    GAAATCCATCATTTCTTTGATGGCATTTATGGTTCTAGCCCTGAAGCACCCCATAAGGCA
    GATGTCATTCACCAAGCCTTGCAGACACATCAACTAGTCCCTCAACAAGCCATCATCATC
    GGAGATACCAAGTTTGATATGCTGGGAGCTCGAGAAACAGGTATTCAGAAATTGGCCGTC
    ACTTGGGGATTTGGAGAGCAAGCAGATCTACTAAACTATCAACCTGATTATATCGCTCAC
    AAACCCTTAGAGGTTTTGGCGTATTTTCAATAG
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    633

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SPOH_0092 [new locus tag: EL271_RS00540 ]
  • symbol: SPOH_0092
  • description: 5'-nucleotidase
  • length: 210
  • theoretical pI: 4.83031
  • theoretical MW: 23405.4
  • GRAVY: -0.113333

⊟Function[edit | edit source]

  • ⊞TIGRFAM:
    AHBA synthesis associated protein (TIGR01454; HMM-score: 76.3)
    Metabolism Energy metabolism Sugars phosphoglycolate phosphatase, bacterial (TIGR01449; EC 3.1.3.18; HMM-score: 64.8)
    and 22 more
    HAD hydrolase, TIGR02253 family (TIGR02253; HMM-score: 56.4)
    noncanonical pyrimidine nucleotidase, YjjG family (TIGR02254; EC 3.1.3.5; HMM-score: 39.4)
    Unknown function Enzymes of unknown specificity HAD hydrolase, family IA, variant 1 (TIGR01549; HMM-score: 37.8)
    beta-phosphoglucomutase family hydrolase (TIGR02009; HMM-score: 33.9)
    haloacid dehalogenase, type II (TIGR01428; EC 3.8.1.2; HMM-score: 32.7)
    Metabolism Energy metabolism Biosynthesis and degradation of polysaccharides beta-phosphoglucomutase (TIGR01990; EC 5.4.2.6; HMM-score: 31.2)
    pyrimidine 5'-nucleotidase (TIGR01993; EC 3.1.3.5; HMM-score: 30.3)
    Unknown function Enzymes of unknown specificity HAD hydrolase, family IIIA (TIGR01662; HMM-score: 28.8)
    Unknown function Enzymes of unknown specificity HAD hydrolase, family IA, variant 3 (TIGR01509; HMM-score: 26.6)
    HAD hydrolase, REG-2-like, family IA (TIGR02252; HMM-score: 26.4)
    histidinol-phosphate phosphatase domain (TIGR01656; HMM-score: 24.8)
    Unknown function Enzymes of unknown specificity Cof-like hydrolase (TIGR00099; HMM-score: 22.8)
    Unknown function Enzymes of unknown specificity HAD hydrolase, family IIB (TIGR01484; HMM-score: 20.4)
    phosphonatase-like hydrolase (TIGR03351; HMM-score: 19.5)
    HAD phosphatase, family IIIA (TIGR01668; EC 3.1.3.-; HMM-score: 17.5)
    Unknown function Enzymes of unknown specificity HAD phosphoserine phosphatase-like hydrolase, family IB (TIGR01488; HMM-score: 16.8)
    FCP1-like phosphatase, phosphatase domain (TIGR02250; EC 3.1.3.16; HMM-score: 15.4)
    Unknown function Enzymes of unknown specificity HAD hydrolase, family IIA (TIGR01460; HMM-score: 14.1)
    Metabolism Central intermediary metabolism Other phosphonoacetaldehyde hydrolase (TIGR01422; EC 3.11.1.1; HMM-score: 13.7)
    Cell structure Cell envelope Biosynthesis and degradation of surface polysaccharides and lipopolysaccharides D,D-heptose 1,7-bisphosphate phosphatase (TIGR00213; HMM-score: 13.3)
    Cell structure Cell envelope Biosynthesis and degradation of surface polysaccharides and lipopolysaccharides 3-deoxy-D-manno-octulosonate 8-phosphate phosphatase, YrbI family (TIGR01670; EC 3.1.3.45; HMM-score: 13.2)
    HAD phosphatase, family IIIC (TIGR01681; HMM-score: 12.6)
  • TheSEED: data available for D39, Hungary19A-6, TIGR4
  • ⊞PFAM:
    HAD (CL0137) HAD_2; haloacid dehalogenase-like hydrolase (PF13419; HMM-score: 125.9)
    and 7 more
    Hydrolase; haloacid dehalogenase-like hydrolase (PF00702; HMM-score: 35.3)
    Hydrolase_like; HAD-hyrolase-like (PF13242; HMM-score: 35.1)
    HAD; haloacid dehalogenase-like hydrolase (PF12710; HMM-score: 34.6)
    Hydrolase_3; haloacid dehalogenase-like hydrolase (PF08282; HMM-score: 17.8)
    NIF; NLI interacting factor-like phosphatase (PF03031; HMM-score: 16.2)
    no clan defined DUF3764; Protein of unknown function (DUF3764) (PF12594; HMM-score: 12.3)
    HAD_SAK_2; HAD domain in Swiss Army Knife RNA repair proteins (PF18143; HMM-score: 12.1)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • ⊞PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • ⊞DeepLocPro: Cytoplasmic
    • Cytoplasmic Score: 0.9978
    • Cytoplasmic Membrane Score: 0.001
    • Cell wall & surface Score: 0.0002
    • Extracellular Score: 0.001
  • ⊞SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.006501
    • TAT(Tat/SPI): 0.000545
    • LIPO(Sec/SPII): 0.000717
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

  • GI:
  • RefSeq: BBG36124 NCBI
  • UniProt:

⊟Protein sequence[edit | edit source]

  • MTSITAIFFDLDGTLVDSSIGIHNAFTYTFKELGVPSPDAKTIRGFMGPPLESSFATCLSKDQISEAVQIYRSYYKAKGIYEAQLFPQIIDLLEELSSSYPLYITTTKNTSTAQDMAKNLEIHHFFDGIYGSSPEAPHKADVIHQALQTHQLVPQQAIIIGDTKFDMLGARETGIQKLAVTWGFGEQADLLNYQPDYIAHKPLEVLAYFQ

⊟Experimental data[edit | edit source]

  • protein localization:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator:

⊟Expression data[edit | edit source]

  • PneumoExpress for strain D39V: SPV_0101 PneumoExpress

⊞Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊞Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

⊟Relevant publications[edit | edit source]

Retrieved from "http://pneumowiki.med.uni-greifswald.de/index.php?title=SPOH_0092&oldid=81745"
  • This page was last edited on 20 January 2022, at 16:12.
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