⊟Summary[edit | edit source]

PangenomeTIGR4

serotype 4

D39

serotype 2

D39V

serotype 2

Hungary19A-6

serotype 19A

EF3030

serotype 19F

670-6B

serotype 6B

6A-10

serotype 6A

70585

serotype 5

A026

serotype 19F

A66

serotype 3

AP200

serotype 11A

ASP0581

serotype 12F

ATCC 49619

serotype 19F

ATCC 700669

serotype 23F

BVJ1JL

serotype 1

CGSP14

serotype 14

G54

serotype 19F

HU-OH

serotype 3

Hu15

serotype 19A

Hu17

serotype 19A

INV104

serotype 1

INV200

serotype 14

JJA

serotype 14

MDRSPN001

serotype 19F

NCTC7466

serotype 2

NU83127

serotype 4

OXC141

serotype 3

P1031

serotype 1

R6

serotype 2

SP49

serotype 19A

SPN032672

serotype 1

SPN034156

serotype 3

SPN034183

serotype 3

SPN994038

serotype 3

SPN994039

serotype 3

SPNA45

serotype 3

ST556

serotype 19F

TCH8431/19A

serotype 19A

Taiwan19F-14

serotype 19F

Xen35

serotype 4

gamPNI0373

serotype 1

NCBI: 26-AUG-2017

⊟Summary

• organism: Streptococcus pneumoniae OXC141
locus tag: SPNOXC00860 [new locus tag: SPNOXC_RS00505 ]
pan locus tag^?: PNEUPAN000692000
symbol: purF
pan gene symbol^?: purF
• synonym:
• product: putative amidophosphoribosyltransferase precursor

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: SPNOXC00860 [new locus tag: SPNOXC_RS00505 ]
• symbol: purF
• product: putative amidophosphoribosyltransferase precursor
• replicon: chromosome
• strand: +
• coordinates: 82824..84266
• length: 1443
• essential: unknown

⊟Accession numbers

• Location: FQ312027 (82824..84266) NCBI
• BioCyc: see SPNOXC_RS00505
• MicrobesOnline:
• PneumoBrowse for strain D39V: SPV_0053 PneumoBrowse

⊟Phenotype

Share your knowledge and add information here. [edit]

⊟DNA sequence

• 1
  61
  121
  181
  241
  301
  361
  421
  481
  541
  601
  661
  721
  781
  841
  901
  961
  1021
  1081
  1141
  1201
  1261
  1321
  1381
  1441
  
  ATGACATACGAAGTAAAATCTCTTAATGAAGAATGTGGTGTTTTCGGTATTTGGGGACAT
  CCAGATGCTGCTAAGTTGACCTATTTTGGACTCCACAGTCTTCAACACCGTGGTCAGGAG
  GGGGCAGGAATCCTCTCCAACGATCAAGGACAACTGAAGCGCCATCGTGACATGGGGCTT
  TTATCAGAAGTTTTCAGAAATCCAGCTAATTTGGATAAATTGACAGGAGCTGGTGCGATT
  GGGCATGTGCGTTATGCGACTGCTGGCGAAGCTTCTGTAGATAACATCCAGCCCTTCCTC
  TTCCGTTTTCACGATATGCAGTTTGGCTTGGCTCATAATGGAAATCTGACCAATGCAGCC
  TCTCTCAAGAAAGAACTGGAACAAAGAGGAGCAATTTTCAGCGCGACTTCGGACTCTGAA
  ATCTTGGCTCACCTCATTCGTCGCAGTCATAATCCTAGCCTGATGGGCAAAATCAAGGAA
  GCGCTCAGCCTTGTCAAAGGTGGTTTTGCCTATATCTTGCTGTTTGAGGACAAGTTGATT
  GCGGCTCTTGACCCTAATGGCTTTCGTCCGCTTTCTATCGGGAAAATGGCCAACGGAGCG
  GTGGTTGTTTCCTCTGAAACCTGTGCTTTTGAGGTCATTGGTGCCGAGTGGATTCGTGAT
  TTGAAGCCAGGTGAGATTGTGATCATTGATGACGAGGGCATTCAGTATGACAGCTATACA
  GATGATACCCAGTTGGCGATTTGTTCTATGGAGTATATCTACTTTGCCCGCCCTGATTCT
  AATATCCACGGTGTCAATGTCCATACGGCACGTAAGAGAATGGGAGCGCAATTGGCGCGA
  GAATTTAAGCATGAGGCAGATATTGTAGTTGGTGTGCCCAATTCTTCCCTAAGCGCGGCT
  ATGGGATTTGCGGAAGAATCAGGCTTACCAAATGAAATGGGTCTGATCAAAAACCAATAC
  ACCCAGCGAACTTTTATCCAACCGACTCAAGAATTGCGGGAGCAAGGAGTGCGGATGAAA
  CTGTCTGCTGTTTCGGGTGTTGTCAAAGGCAAACGTGTGGTCATGGTGGATGATTCCATT
  GTACGTGGAACAACCTCTCGTCGTATCGTTCAGCTCTTGAAAGAAGCGGGTGCGACTGAG
  GTTCACGTTGCCATTGGAAGTCCTGCACTAGCGTATCCATGTTTCTACGGGATTGATATC
  CAGACCCGTCAGGAGCTGATTGCAGCCAATCATACGGTCGAAGAAACTCGCCAAATCATT
  GGTGCGGACAGTCTGACTTATCTTTCAATTGATAGCTTGATTGAGTCGATTGGTATCGAA
  ACAGATGCGCCGAACGGTGGTCTCTGTGTCGCTTACTTTGACGGTGACTACCCAACGCCT
  CTCTATGACTACGAAGAAGACTATCGTAGAAGTTTGGAAGAAAAGACCAGTTTTTACAAG
  TAG
  60
  120
  180
  240
  300
  360
  420
  480
  540
  600
  660
  720
  780
  840
  900
  960
  1020
  1080
  1140
  1200
  1260
  1320
  1380
  1440
  1443
  

⊟Protein

⊟General

• locus tag: SPNOXC00860 [new locus tag: SPNOXC_RS00505 ]
• symbol: PurF
• description: putative amidophosphoribosyltransferase precursor
• length: 480
• theoretical pI: 5.46344
• theoretical MW: 52833.5
• GRAVY: -0.195833

⊟Function

• ⊞⊞TIGRFAM:
  Metabolism Purines, pyrimidines, nucleosides, and nucleotides Purine ribonucleotide biosynthesis amidophosphoribosyltransferase (TIGR01134; EC 2.4.2.14; HMM-score: 513.4)
  and 14 more
  Cell structure Cell envelope Biosynthesis and degradation of murein sacculus and peptidoglycan glutamine-fructose-6-phosphate transaminase (isomerizing) (TIGR01135; EC 2.6.1.16; HMM-score: 104.8)
  Metabolism Central intermediary metabolism Amino sugars glutamine-fructose-6-phosphate transaminase (isomerizing) (TIGR01135; EC 2.6.1.16; HMM-score: 104.8)
  Metabolism Amino acid biosynthesis Aspartate family asparagine synthase (glutamine-hydrolyzing) (TIGR01536; EC 6.3.5.4; HMM-score: 59)
  Metabolism Purines, pyrimidines, nucleosides, and nucleotides Purine ribonucleotide biosynthesis ribose-phosphate diphosphokinase (TIGR01251; EC 2.7.6.1; HMM-score: 34.5)
  exosortase A system-associated amidotransferase 1 (TIGR03108; HMM-score: 29.3)
  Metabolism Purines, pyrimidines, nucleosides, and nucleotides Pyrimidine ribonucleotide biosynthesis orotate phosphoribosyltransferase (TIGR01367; EC 2.4.2.10; HMM-score: 21.8)
  asparagine synthase family amidotransferase (TIGR03104; HMM-score: 21.5)
  Cellular processes Cellular processes DNA transformation comF family protein (TIGR00201; HMM-score: 21.3)
  Metabolism Purines, pyrimidines, nucleosides, and nucleotides Salvage of nucleosides and nucleotides adenine phosphoribosyltransferase (TIGR01090; EC 2.4.2.7; HMM-score: 17.6)
  Metabolism Purines, pyrimidines, nucleosides, and nucleotides Salvage of nucleosides and nucleotides xanthine phosphoribosyltransferase (TIGR01744; EC 2.4.2.22; HMM-score: 15.2)
  Metabolism Purines, pyrimidines, nucleosides, and nucleotides Salvage of nucleosides and nucleotides hypoxanthine phosphoribosyltransferase (TIGR01203; EC 2.4.2.8; HMM-score: 14.5)
  Metabolism Purines, pyrimidines, nucleosides, and nucleotides Pyrimidine ribonucleotide biosynthesis orotate phosphoribosyltransferase (TIGR00336; EC 2.4.2.10; HMM-score: 12.7)
  Metabolism Purines, pyrimidines, nucleosides, and nucleotides Purine ribonucleotide biosynthesis pur operon repressor PurR (TIGR01743; HMM-score: 12.1)
  Signal transduction Regulatory functions DNA interactions pur operon repressor PurR (TIGR01743; HMM-score: 12.1)
• ⊞⊞TheSEED  :
  • Amidophosphoribosyltransferase (EC 2.4.2.14)
  Nucleosides and Nucleotides Purines De Novo Purine Biosynthesis  Amidophosphoribosyltransferase (EC 2.4.2.14)
  and 1 more
  Virulence, Disease and Defense Bacteriocins, ribosomally synthesized antibacterial peptides Colicin V and Bacteriocin Production Cluster  Amidophosphoribosyltransferase (EC 2.4.2.14)
• ⊞⊞PFAM:
  NTN (CL0052) GATase_6; Glutamine amidotransferase domain (PF13522; HMM-score: 64.7)
  GATase_7; Glutamine amidotransferase domain (PF13537; HMM-score: 64.6)
  and 5 more
  PRTase-like (CL0533) Pribosyltran; Phosphoribosyl transferase domain (PF00156; HMM-score: 38.5)
  NTN (CL0052) GATase_4; Glutamine amidotransferases class-II (PF13230; HMM-score: 31.3)
  GATase_2; Glutamine amidotransferases class-II (PF00310; HMM-score: 17.8)
  PRTase-like (CL0533) Pribosyl_synth; Phosphoribosyl synthetase-associated domain (PF14572; HMM-score: 15.6)
  NTN (CL0052) DUF3700; Aluminium induced protein (PF12481; HMM-score: 12.5)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• ⊞⊞PSORTb: Cytoplasmic
  • Cytoplasmic Score: 9.97
  • Cytoplasmic Membrane Score: 0
  • Cellwall Score: 0.01
  • Extracellular Score: 0.02
  • Internal Helices: 0
• ⊞⊞SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.006184
  • TAT(Tat/SPI): 0.000421
  • LIPO(Sec/SPII): 0.001276
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI:
• RefSeq: CBW31747 NCBI
• UniProt:

⊟Protein sequence

• MTYEVKSLNEECGVFGIWGHPDAAKLTYFGLHSLQHRGQEGAGILSNDQGQLKRHRDMGLLSEVFRNPANLDKLTGAGAIGHVRYATAGEASVDNIQPFLFRFHDMQFGLAHNGNLTNAASLKKELEQRGAIFSATSDSEILAHLIRRSHNPSLMGKIKEALSLVKGGFAYILLFEDKLIAALDPNGFRPLSIGKMANGAVVVSSETCAFEVIGAEWIRDLKPGEIVIIDDEGIQYDSYTDDTQLAICSMEYIYFARPDSNIHGVNVHTARKRMGAQLAREFKHEADIVVGVPNSSLSAAMGFAEESGLPNEMGLIKNQYTQRTFIQPTQELREQGVRMKLSAVSGVVKGKRVVMVDDSIVRGTTSRRIVQLLKEAGATEVHVAIGSPALAYPCFYGIDIQTRQELIAANHTVEETRQIIGADSLTYLSIDSLIESIGIETDAPNGGLCVAYFDGDYPTPLYDYEEDYRRSLEEKTSFYK

⊟Experimental data

• protein localization:
• interaction partners:

⊟Expression & Regulation

⊟Operon

⊟Regulation

• data available for TIGR4

⊟Expression data

• PneumoExpress for strain D39V: SPV_0053 PneumoExpress

⊞Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊞Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications