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PangenomeTIGR4
serotype 4
D39
serotype 2
D39V
serotype 2
Hungary19A-6
serotype 19A
EF3030
serotype 19F
670-6B
serotype 6B
6A-10
serotype 6A
70585
serotype 5
A026
serotype 19F
A66
serotype 3
AP200
serotype 11A
ASP0581
serotype 12F
ATCC 49619
serotype 19F
ATCC 700669
serotype 23F
BVJ1JL
serotype 1
CGSP14
serotype 14
G54
serotype 19F
HU-OH
serotype 3
Hu15
serotype 19A
Hu17
serotype 19A
INV104
serotype 1
INV200
serotype 14
JJA
serotype 14
MDRSPN001
serotype 19F
NCTC7466
serotype 2
NU83127
serotype 4
OXC141
serotype 3
P1031
serotype 1
R6
serotype 2
SP49
serotype 19A
SPN032672
serotype 1
SPN034156
serotype 3
SPN034183
serotype 3
SPN994038
serotype 3
SPN994039
serotype 3
SPNA45
serotype 3
ST556
serotype 19F
TCH8431/19A
serotype 19A
Taiwan19F-14
serotype 19F
Xen35
serotype 4
gamPNI0373
serotype 1

NCBI: 24-APR-2024

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⊟Summary[edit | edit source]

Contents

  • 1 Summary
  • 2 Genome View
  • 3 Gene
    • 3.1 General
    • 3.2 Accession numbers
    • 3.3 Phenotype
    • 3.4 DNA sequence
  • 4 Protein
    • 4.1 General
    • 4.2 Function
    • 4.3 Structure, modifications & cofactors
    • 4.4 Localization
    • 4.5 Accession numbers
    • 4.6 Protein sequence
    • 4.7 Experimental data
  • 5 Expression & Regulation
    • 5.1 Operon
    • 5.2 Regulation
    • 5.3 Expression data
  • 6 Biological Material
    • 6.1 Mutants
    • 6.2 Expression vector
    • 6.3 lacZ fusion
    • 6.4 GFP fusion
    • 6.5 two-hybrid system
    • 6.6 FLAG-tag construct
    • 6.7 Antibody
  • 7 Other Information
  • 8 Literature
    • 8.1 References
    • 8.2 Relevant publications
  • organism: Streptococcus pneumoniae G54
  • locus tag: SPG_RS05105 [old locus tag: SPG_1014 ]
  • pan locus tag?: PNEUPAN002258000
  • symbol: SPG_RS05105
  • pan gene symbol?: nifS
  • synonym:
  • product: cysteine desulfurase family protein

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SPG_RS05105 [old locus tag: SPG_1014 ]
  • symbol: SPG_RS05105
  • product: cysteine desulfurase family protein
  • replicon: chromosome
  • strand: -
  • coordinates: 974181..975296
  • length: 1116
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

  • Location: NC_011072 (974181..975296) NCBI
  • BioCyc: SPG_RS05105 BioCyc
  • MicrobesOnline: see SPG_1014
  • PneumoBrowse for strain D39V: SPV_0979 PneumoBrowse

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    TTGATTTATTTGGACAATGCTGCAACGACTCCTATGTCAGCAGTTGCTATTTCAGCTATG
    ACCAAGGTTATGCAAGAAACCCACGGAAATCCTTCTAGTATTCATGGTCATGGTCGTCAA
    GCTGGCAAACTCTTGCGAGAAGCCCGTCAGGAACTAGCCCAGTTACTAAGGACAAAACCT
    CAACATATCTTTTTCACTTCTGGTGGGACTGAAGGCAATAATACTACCATCATTGGCTAC
    TGTCTTCGTCACCAAGAACAAGGAAAACATATCATCACAACTGCCATCGAGCACCATGCT
    GTCCTTGAAACAATTGATTACTTGGTTCAACACTTTGGGTTTGAAGCAACCATTATCCAG
    CCAGAAAATCAAGAAATCACAGCCCAGCAAATTCAAAAGGCTTTACGTGACGATACGATT
    TTGGTTTCTACTATGTTTGCCAATAATGAGACAGGAAACCTACTTCCCATCGCTGAAATT
    GGCCAAATACTCAAGCAACACCCTGCTGCCTATCATGTTGATGCAGTTCAGGCTATTGGT
    AAAATCCCAATTCATTCAGAAGAATTGGGCATTGATTTTCTCACTGCTTCTGCCCACAAA
    TTCCATGGTCCTAAGGGAATCGGTTTTCTCTACGCATCTAGCATGGACTTTGATTCCTAT
    CTACATGGCGGAGATCAGGAACAGAAAAAACGTGCAGGAACTGAAAATCTGCCTGCCATC
    GTAGGCATGGTTGCAGCCCTAAAAGAAGACCTAGAAAAACAAGAAGAACATTTTCAACAT
    GTACAAAATCTAGAAACTGCCTTTCTGGCAGAGCTGGAGGGCATTCAGTATTACTTGAAT
    AGAGGAAAACATCATCTCCCTTATGTTCTCAATATTGGATTTCCTGGTCAGAAAAATGAC
    CTCTTACTCCTTCGGCTAGATTTAGCTGGAATTTCAATCTCTACTGGCTCAGCCTGTACT
    GCAGGCGTTGTCCAATCCAGCCATGTTCTTAAAGCCATGCATGGCGCAAATTCAGAACGC
    TTGAAGGAATCCCTTCGCATCAGTTTGTCGCCACAAAATACCGTTGAAGACCTACAAACC
    CTCGCAAAAACCTTAAAAGAAATTATCGGAGGTTAG
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1116

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SPG_RS05105 [old locus tag: SPG_1014 ]
  • symbol: SPG_RS05105
  • description: cysteine desulfurase family protein
  • length: 371
  • theoretical pI: 6.54894
  • theoretical MW: 40708
  • GRAVY: -0.219946

⊟Function[edit | edit source]

  • ⊞TIGRFAM:
    cysteine desulfurase NifS (TIGR03402; EC 2.8.1.7; HMM-score: 367.6)
    Genetic information processing DNA metabolism Restriction/modification cysteine desulfurase DndA (TIGR03235; EC 2.8.1.7; HMM-score: 306.1)
    and 17 more
    Genetic information processing Protein synthesis tRNA and rRNA base modification cysteine desulfurase IscS (TIGR02006; EC 2.8.1.7; HMM-score: 278.3)
    Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Other cysteine desulfurase IscS (TIGR02006; EC 2.8.1.7; HMM-score: 278.3)
    cysteine desulfurase, NifS family (TIGR03403; EC 2.8.1.7; HMM-score: 245.8)
    Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Other cysteine desulfurase, SufS family (TIGR01979; HMM-score: 154.4)
    Unknown function Enzymes of unknown specificity cysteine desulfurase family protein (TIGR01977; HMM-score: 142.8)
    Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Other cysteine desulfurase, catalytic subunit CsdA (TIGR03392; EC 2.8.1.7; HMM-score: 93.2)
    Unknown function Enzymes of unknown specificity cysteine desulfurase family protein (TIGR01976; HMM-score: 81.5)
    2-aminoethylphosphonate aminotransferase (TIGR03301; EC 2.6.1.-; HMM-score: 57.1)
    Metabolism Central intermediary metabolism Phosphorus compounds 2-aminoethylphosphonate--pyruvate transaminase (TIGR02326; EC 2.6.1.37; HMM-score: 49.4)
    Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Glutathione and analogs ergothioneine biosynthesis PLP-dependent enzyme EgtE (TIGR04343; EC 4.4.-.-; HMM-score: 34.6)
    Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Other tyrosine decarboxylase MnfA (TIGR03812; EC 4.1.1.25; HMM-score: 31.7)
    O-phospho-L-seryl-tRNA:Cys-tRNA synthase (TIGR02539; EC 2.5.1.73; HMM-score: 26.7)
    Metabolism Amino acid biosynthesis Aspartate family O-acetylhomoserine aminocarboxypropyltransferase/cysteine synthase (TIGR01326; HMM-score: 21.8)
    Metabolism Amino acid biosynthesis Serine family O-acetylhomoserine aminocarboxypropyltransferase/cysteine synthase (TIGR01326; HMM-score: 21.8)
    beta-methylarginine biosynthesis bifunctional aminotransferase (TIGR04544; EC 2.6.-.-; HMM-score: 18.6)
    Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Heme, porphyrin, and cobalamin 5-aminolevulinic acid synthase (TIGR01821; EC 2.3.1.37; HMM-score: 17.3)
    Metabolism Amino acid biosynthesis Serine family phosphoserine transaminase (TIGR01364; EC 2.6.1.52; HMM-score: 14.1)
  • TheSEED: see SPG_1014
  • ⊞PFAM:
    PLP_aminotran (CL0061) Aminotran_5; Aminotransferase class-V (PF00266; HMM-score: 247)
    and 2 more
    Cys_Met_Meta_PP; Cys/Met metabolism PLP-dependent enzyme (PF01053; HMM-score: 23)
    Ank (CL0465) DUF3447; Domain of unknown function (DUF3447) (PF11929; HMM-score: 17.7)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • ⊞PSORTb: Cytoplasmic
    • Cytoplasmic Score: 9.97
    • Cytoplasmic Membrane Score: 0
    • Cellwall Score: 0.01
    • Extracellular Score: 0.02
    • Internal Helices: 0
  • ⊞SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.05651
    • TAT(Tat/SPI): 0.004878
    • LIPO(Sec/SPII): 0.016519
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

  • GI:
  • RefSeq: WP_000639663 NCBI
  • UniProt:

⊟Protein sequence[edit | edit source]

  • MIYLDNAATTPMSAVAISAMTKVMQETHGNPSSIHGHGRQAGKLLREARQELAQLLRTKPQHIFFTSGGTEGNNTTIIGYCLRHQEQGKHIITTAIEHHAVLETIDYLVQHFGFEATIIQPENQEITAQQIQKALRDDTILVSTMFANNETGNLLPIAEIGQILKQHPAAYHVDAVQAIGKIPIHSEELGIDFLTASAHKFHGPKGIGFLYASSMDFDSYLHGGDQEQKKRAGTENLPAIVGMVAALKEDLEKQEEHFQHVQNLETAFLAELEGIQYYLNRGKHHLPYVLNIGFPGQKNDLLLLRLDLAGISISTGSACTAGVVQSSHVLKAMHGANSERLKESLRISLSPQNTVEDLQTLAKTLKEIIGG

⊟Experimental data[edit | edit source]

  • protein localization:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

  • MicrobesOnline: SPG_RS05085 < SPG_RS05090 < SPG_RS05095 < SPG_RS05100 < SPG_RS05105 < SPG_RS05110 < SPG_RS05115

⊟Regulation[edit | edit source]

  • regulator:

⊟Expression data[edit | edit source]

  • PneumoExpress for strain D39V: SPV_0979 PneumoExpress

⊞Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊞Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

⊟Relevant publications[edit | edit source]

Retrieved from "http://pneumowiki.med.uni-greifswald.de/index.php?title=SPG_RS05105&oldid=71856"
  • This page was last edited on 20 January 2022, at 15:49.
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