⊟Summary[edit | edit source]

PangenomeTIGR4

serotype 4

D39

serotype 2

D39V

serotype 2

Hungary19A-6

serotype 19A

EF3030

serotype 19F

670-6B

serotype 6B

6A-10

serotype 6A

70585

serotype 5

A026

serotype 19F

A66

serotype 3

AP200

serotype 11A

ASP0581

serotype 12F

ATCC 49619

serotype 19F

ATCC 700669

serotype 23F

BVJ1JL

serotype 1

CGSP14

serotype 14

G54

serotype 19F

HU-OH

serotype 3

Hu15

serotype 19A

Hu17

serotype 19A

INV104

serotype 1

INV200

serotype 14

JJA

serotype 14

MDRSPN001

serotype 19F

NCTC7466

serotype 2

NU83127

serotype 4

OXC141

serotype 3

P1031

serotype 1

R6

serotype 2

SP49

serotype 19A

SPN032672

serotype 1

SPN034156

serotype 3

SPN034183

serotype 3

SPN994038

serotype 3

SPN994039

serotype 3

SPNA45

serotype 3

ST556

serotype 19F

TCH8431/19A

serotype 19A

Taiwan19F-14

serotype 19F

Xen35

serotype 4

gamPNI0373

serotype 1

NCBI: 17-JAN-2018

⊟Summary

• organism: Streptococcus pneumoniae AP200
locus tag: SPAP_1714 [new locus tag: SPAP_RS08490 ]
pan locus tag^?: PNEUPAN003118000
symbol: SPAP_1714
pan gene symbol^?: engA
• synonym: der
• product: Predicted GTPase

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: SPAP_1714 [new locus tag: SPAP_RS08490 ]
• symbol: SPAP_1714
• product: Predicted GTPase
• replicon: chromosome
• strand: -
• coordinates: 1620893..1622203
• length: 1311
• essential: unknown other strains

⊟Accession numbers

• Location: CP002121 (1620893..1622203) NCBI
• BioCyc: see SPAP_RS08490
• MicrobesOnline:
• PneumoBrowse for strain D39V: SPV_1519 PneumoBrowse

⊟Phenotype

Share your knowledge and add information here. [edit]

⊟DNA sequence

• 1
  61
  121
  181
  241
  301
  361
  421
  481
  541
  601
  661
  721
  781
  841
  901
  961
  1021
  1081
  1141
  1201
  1261
  
  ATGGCCCTACCAACTATTGCCATTGTAGGACGTCCCAATGTTGGGAAATCAACCCTATTT
  AATCGGATCGCTGGTGAGCGAATCTCCATTGTAGAAGATGTCGAAGGAGTGACACGTGAC
  CGTATTTATGCAACGGGTGAGTGGCTCAATCGTTCTTTTAGCATGATTGATACAGGAGGA
  ATTGATGATGTCGATGCTCCTTTCATGGAACAAATCAAGCACCAGGCAGAAATTGCCATG
  GAAGAAGCAGATGTTATCGTCTTTGTCGTGTCCGGTAAGGAAGGAATTACCGATGCAGAC
  GAATACGTAGCCCGTAAGCTTTATAAGACCCACAAACCAGTTATCCTCGCAGTTAACAAG
  GTGGACAACCCTGAGATGCGAAATGATATCTATGATTTCTATGCCCTCGGTTTGGGTGAA
  CCACTGCCTATCTCATCTGTCCATGGTATCGGTACAGGGGATGTACTAGATGCGATTGTG
  GAAAACCTTCCAAATGAATATGAAGAAGAAAATCCAGATGTCATTAAGTTTAGCCTGATT
  GGTCGTCCAAACGTTGGAAAATCAAGCTTGATCAATGCTATCTTGGGCGAAGACCGTGTT
  ATTGCTAGTCCTGTTGCTGGAACAACTCGTGATGCCATTGATACCCACTTTACAGATACA
  GATGGTCAAGAGTTTACCATGATTGATACGGCTGGTATGCGTAAGTCTGGTAAGGTTTAT
  GAAAATACTGAGAAATACTCTGTTATGCGTGCCATGCGTGCTATTGACCGTTCAGATGTG
  GTCTTGATGGTCATCAATGCGGAAGAAGGCATTCGTGAGTACGACAAGCGTATCGCAGGA
  TTTGCCCATGAAGCTGGTAAAGGGATGATTATCGTGGTCAACAAGTGGGATACGCTTGAA
  AAAGATAACCACACTATGAAAAACTGGGAAGAAGATATCCGTGAGCAGTTCCAATACCTG
  CCTTACGCACCGATTATCTTTGTATCAGCTTTAACCAAGCAACGTCTCCACAAACTTCCT
  GAGATGATTAAGCAAATCAGCGAAAGTCAAAATACACGTATTCCATCAGCTGTCTTGAAC
  GATGTCATCATGGATGCCATTGCCATCAACCCAACACCGACAGACAAAGGAAAACGTCTC
  AAGATTTTCTATGCGACCCAAGTGGCAACCAAACCACCAACCTTTGTCATCTTTGTCAAT
  GAAGAAGAACTCATGCACTTTTCTTACCTGCGTTTCTTGGAAAATCAAATCCGCAAGGCC
  TTTGTTTTTGAGGGAACACCGATTCATCTCATCGCAAGAAAACGCAAATAA
  60
  120
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  420
  480
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  1020
  1080
  1140
  1200
  1260
  1311
  

⊟Protein

⊟General

• locus tag: SPAP_1714 [new locus tag: SPAP_RS08490 ]
• symbol: SPAP_1714
• description: Predicted GTPase
• length: 436
• theoretical pI: 5.07777
• theoretical MW: 49081.8
• GRAVY: -0.237615

⊟Function

• ⊞⊞TIGRFAM:
  Genetic information processing Protein synthesis Other ribosome-associated GTPase EngA (TIGR03594; HMM-score: 577.7)
  and 29 more
  Genetic information processing Protein fate Protein modification and repair [FeFe] hydrogenase H-cluster maturation GTPase HydF (TIGR03918; HMM-score: 174.9)
  Genetic information processing Protein synthesis Other GTP-binding protein Era (TIGR00436; HMM-score: 172.7)
  Unknown function General small GTP-binding protein domain (TIGR00231; HMM-score: 169.4)
  Genetic information processing Protein synthesis tRNA and rRNA base modification tRNA modification GTPase TrmE (TIGR00450; EC 3.6.-.-; HMM-score: 165.6)
  Metabolism Transport and binding proteins Cations and iron carrying compounds ferrous iron transport protein B (TIGR00437; HMM-score: 102)
  Genetic information processing Protein synthesis Other ribosome biogenesis GTP-binding protein YlqF (TIGR03596; HMM-score: 92)
  Genetic information processing Protein synthesis Other ribosome biogenesis GTP-binding protein YsxC (TIGR03598; HMM-score: 77.3)
  Genetic information processing Protein synthesis Other ribosome biogenesis GTPase YqeH (TIGR03597; HMM-score: 65.9)
  Unknown function General GTP-binding protein HflX (TIGR03156; HMM-score: 64.5)
  Genetic information processing Protein synthesis Translation factors translation initiation factor IF-2 (TIGR00487; HMM-score: 55.6)
  Genetic information processing Protein synthesis Other Obg family GTPase CgtA (TIGR02729; HMM-score: 46.6)
  Genetic information processing Protein synthesis Translation factors ribosome small subunit-dependent GTPase A (TIGR00157; EC 3.6.-.-; HMM-score: 37.2)
  Cellular processes Cellular processes Adaptations to atypical conditions GTP-binding protein TypA/BipA (TIGR01394; HMM-score: 30.8)
  Genetic information processing Protein synthesis Translation factors GTP-binding protein TypA/BipA (TIGR01394; HMM-score: 30.8)
  Signal transduction Regulatory functions Other GTP-binding protein TypA/BipA (TIGR01394; HMM-score: 30.8)
  Unknown function General GTP-binding protein YchF (TIGR00092; HMM-score: 30.1)
  Metabolism Transport and binding proteins Nucleosides, purines and pyrimidines GTP-binding protein (TIGR00991; HMM-score: 28.7)
  Metabolism Energy metabolism Amino acids and amines ethanolamine utilization protein, EutP (TIGR02528; HMM-score: 26.8)
  Unknown function General elongation factor 4 (TIGR01393; EC 3.6.5.-; HMM-score: 25.2)
  Genetic information processing Protein synthesis Translation factors selenocysteine-specific translation elongation factor (TIGR00475; HMM-score: 23.9)
  Metabolism Transport and binding proteins Amino acids, peptides and amines chloroplast protein import component Toc86/159, G and M domains (TIGR00993; HMM-score: 23.2)
  Metabolism Central intermediary metabolism Sulfur metabolism sulfate adenylyltransferase, large subunit (TIGR02034; EC 2.7.7.4; HMM-score: 20.5)
  Genetic information processing Protein synthesis Translation factors translation elongation factor aEF-2 (TIGR00490; HMM-score: 17.9)
  Metabolism Purines, pyrimidines, nucleosides, and nucleotides Nucleotide and nucleoside interconversions guanylate kinase (TIGR03263; EC 2.7.4.8; HMM-score: 14.9)
  Metabolism Transport and binding proteins Amino acids, peptides and amines urea ABC transporter, ATP-binding protein UrtE (TIGR03410; HMM-score: 14.4)
  Genetic information processing Protein fate Protein modification and repair hydrogenase accessory protein HypB (TIGR00073; HMM-score: 13.7)
  Metabolism Transport and binding proteins Other thiamine ABC transporter, ATP-binding protein (TIGR01277; EC 3.6.3.-; HMM-score: 12.5)
  Hypothetical proteins Conserved TIGR00703 family protein (TIGR00703; HMM-score: 12.4)
  Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Pantothenate and coenzyme A dephospho-CoA kinase (TIGR00152; EC 2.7.1.24; HMM-score: 11.9)
• TheSEED  :
  • GTP-binding protein EngA
  Protein Metabolism Protein biosynthesis Universal GTPases  GTP-binding protein EngA
• ⊞⊞PFAM:
  P-loop_NTPase (CL0023) MMR_HSR1; 50S ribosome-binding GTPase (PF01926; HMM-score: 179)
  and 31 more
  no clan defined KH_dom-like; KH-domain-like of EngA bacterial GTPase enzymes, C-terminal (PF14714; HMM-score: 115.5)
  P-loop_NTPase (CL0023) FeoB_N; Ferrous iron transport protein B (PF02421; HMM-score: 99.8)
  GTP_EFTU; Elongation factor Tu GTP binding domain (PF00009; HMM-score: 83.7)
  AIG1; AIG1 family (PF04548; HMM-score: 68.8)
  Dynamin_N; Dynamin family (PF00350; HMM-score: 58.3)
  RsgA_GTPase; RsgA GTPase (PF03193; HMM-score: 42.5)
  Roc; Ras of Complex, Roc, domain of DAPkinase (PF08477; HMM-score: 34.1)
  PduV-EutP; Ethanolamine utilisation - propanediol utilisation (PF10662; HMM-score: 31.2)
  ABC_tran; ABC transporter (PF00005; HMM-score: 29.9)
  SRPRB; Signal recognition particle receptor beta subunit (PF09439; HMM-score: 27.4)
  Ras; Ras family (PF00071; HMM-score: 25.1)
  RNA_helicase; RNA helicase (PF00910; HMM-score: 23.6)
  AAA; ATPase family associated with various cellular activities (AAA) (PF00004; HMM-score: 19.8)
  AAA_18; AAA domain (PF13238; HMM-score: 19.6)
  DUF5906; Family of unknown function (DUF5906) (PF19263; HMM-score: 19.5)
  no clan defined MMR_HSR1_Xtn; C-terminal region of MMR_HSR1 domain (PF16897; HMM-score: 18.4)
  P-loop_NTPase (CL0023) NTPase_1; NTPase (PF03266; HMM-score: 17.8)
  AAA_29; P-loop containing region of AAA domain (PF13555; HMM-score: 17.6)
  cobW; CobW/HypB/UreG, nucleotide-binding domain (PF02492; HMM-score: 17.3)
  Nribosyltransf (CL0498) Nuc_deoxyri_tr2; Nucleoside 2-deoxyribosyltransferase like (PF15891; HMM-score: 16.7)
  P-loop_NTPase (CL0023) NB-ARC; NB-ARC domain (PF00931; HMM-score: 16.5)
  DO-GTPase2; Double-GTPase 2 (PF19993; HMM-score: 15.7)
  TniB; Bacterial TniB protein (PF05621; HMM-score: 15.4)
  MeaB; Methylmalonyl Co-A mutase-associated GTPase MeaB (PF03308; HMM-score: 15.3)
  AAA_24; AAA domain (PF13479; HMM-score: 15.2)
  AAA_16; AAA ATPase domain (PF13191; HMM-score: 15)
  Arf; ADP-ribosylation factor family (PF00025; HMM-score: 14.6)
  ATPase_2; ATPase domain predominantly from Archaea (PF01637; HMM-score: 14.6)
  vWA-like (CL0128) DUF1194; Protein of unknown function (DUF1194) (PF06707; HMM-score: 14.3)
  P-loop_NTPase (CL0023) Gtr1_RagA; Gtr1/RagA G protein conserved region (PF04670; HMM-score: 13.9)
  AAA_22; AAA domain (PF13401; HMM-score: 13.4)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• ⊞⊞PSORTb: Cytoplasmic Membrane
  • Cytoplasmic Score: 1.05
  • Cytoplasmic Membrane Score: 8.78
  • Cellwall Score: 0.08
  • Extracellular Score: 0.09
  • Internal Helices: 0
• ⊞⊞SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.004411
  • TAT(Tat/SPI): 0.00032
  • LIPO(Sec/SPII): 0.000491
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI:
• RefSeq: ADM85298 NCBI
• UniProt:

⊟Protein sequence

• MALPTIAIVGRPNVGKSTLFNRIAGERISIVEDVEGVTRDRIYATGEWLNRSFSMIDTGGIDDVDAPFMEQIKHQAEIAMEEADVIVFVVSGKEGITDADEYVARKLYKTHKPVILAVNKVDNPEMRNDIYDFYALGLGEPLPISSVHGIGTGDVLDAIVENLPNEYEEENPDVIKFSLIGRPNVGKSSLINAILGEDRVIASPVAGTTRDAIDTHFTDTDGQEFTMIDTAGMRKSGKVYENTEKYSVMRAMRAIDRSDVVLMVINAEEGIREYDKRIAGFAHEAGKGMIIVVNKWDTLEKDNHTMKNWEEDIREQFQYLPYAPIIFVSALTKQRLHKLPEMIKQISESQNTRIPSAVLNDVIMDAIAINPTPTDKGKRLKIFYATQVATKPPTFVIFVNEEELMHFSYLRFLENQIRKAFVFEGTPIHLIARKRK

⊟Experimental data

• protein localization:
• interaction partners:

⊟Expression & Regulation

⊟Operon

⊟Regulation

• regulator:

⊟Expression data

• PneumoExpress for strain D39V: SPV_1519 PneumoExpress

⊞Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊞Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications