⊟Summary[edit | edit source]

PangenomeTIGR4

serotype 4

D39

serotype 2

D39V

serotype 2

Hungary19A-6

serotype 19A

EF3030

serotype 19F

670-6B

serotype 6B

6A-10

serotype 6A

70585

serotype 5

A026

serotype 19F

A66

serotype 3

AP200

serotype 11A

ASP0581

serotype 12F

ATCC 49619

serotype 19F

ATCC 700669

serotype 23F

BVJ1JL

serotype 1

CGSP14

serotype 14

G54

serotype 19F

HU-OH

serotype 3

Hu15

serotype 19A

Hu17

serotype 19A

INV104

serotype 1

INV200

serotype 14

JJA

serotype 14

MDRSPN001

serotype 19F

NCTC7466

serotype 2

NU83127

serotype 4

OXC141

serotype 3

P1031

serotype 1

R6

serotype 2

SP49

serotype 19A

SPN032672

serotype 1

SPN034156

serotype 3

SPN034183

serotype 3

SPN994038

serotype 3

SPN994039

serotype 3

SPNA45

serotype 3

ST556

serotype 19F

TCH8431/19A

serotype 19A

Taiwan19F-14

serotype 19F

Xen35

serotype 4

gamPNI0373

serotype 1

NCBI: 22-FEB-2023

⊟Summary

• organism: Streptococcus pneumoniae AP200
locus tag: SPAP_RS09870 [old locus tag: SPAP_1994 ]
pan locus tag^?: PNEUPAN003568000
symbol: rsgA
pan gene symbol^?: rsgA
• synonym:
• product: ribosome small subunit-dependent GTPase A

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: SPAP_RS09870 [old locus tag: SPAP_1994 ]
• symbol: rsgA
• product: ribosome small subunit-dependent GTPase A
• replicon: chromosome
• strand: -
• coordinates: 1862861..1863739
• length: 879
• essential: unknown other strains

⊟Accession numbers

• Location: NC_014494 (1862861..1863739) NCBI
• BioCyc: SPAP_RS09870 BioCyc
• MicrobesOnline:
• PneumoBrowse for strain D39V: SPV_1781 PneumoBrowse

⊟Phenotype

Share your knowledge and add information here. [edit]

⊟DNA sequence

• 1
  61
  121
  181
  241
  301
  361
  421
  481
  541
  601
  661
  721
  781
  841
  
  ATGCAGGGACAAATCATTAAAGCCTTGGCAGGTTTCTACTATGTGGAGAGTGATGGCCAG
  GTTTATCAAACACGCGCGCGTGGGAATTTCCGTAAAAAAGGCCATACCCCTTATGTTGGG
  GACTGGGTAGATTTCTCTGCCGAGGAAAATTCAGAAGGCTATATCCTCAAAATTCACGAA
  CGGAAAAACAGTCTGGTTCGTCCGCCTATTGTCAATATCGATCAAGCTGTAGTAATCATG
  TCCGTCAAGGAACCTGATTTTAACAGCAATTTGCTGGATCGTTTCTTGGTTCTTTTGGAG
  CACAAGGGCATCCATCCCATTGTCTATATTTCCAAAATGGATTTGTTGGAAGATAGGGGA
  GAACTGGATTTTTACCAGCAGACCTATGGTGACATCGGCTATGACTTTGTGACCAGTAAA
  GAGGAACTCCTGTCTTTGTTAACAGGCAAGGTTACGGTCTTTATGGGGCAGACAGGTGTT
  GGGAAGTCAACTCTTCTCAATAAAATCGCACCAGACCTCAATCTTGAAACGGGAGAAATT
  TCAGACAGTCTAGGTCGCGGTCGCCATACCACTCGAGCTGTTAGTTTTTACAATCTCAAC
  GGGGGTAAAATCGCAGATACACCAGGATTTTCATCCTTGGACTATGAAGTATCAAGGGCT
  GAAGACCTCAATCAGGCTTTCCCAGAGATTGCTACTGTTAGCCGAGATTGTAAGTTCCGT
  ACTTGTACCCATACCCATGAGCCGTCTTGTGCCGTCAAACCAGCTGTTGAAGAGGGTGTT
  ATTGCAACCTTCCGTTTTGACAATTACCTGCAATTCCTTAGTGAAATTGAAAATCGTAGA
  GAAACCTATAAAAAAGTCAGCAAAAAAATTCCAAAATAA
  60
  120
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  360
  420
  480
  540
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  840
  879
  

⊟Protein

⊟General

• locus tag: SPAP_RS09870 [old locus tag: SPAP_1994 ]
• symbol: RsgA
• description: ribosome small subunit-dependent GTPase A
• length: 292
• theoretical pI: 6.40805
• theoretical MW: 32993.1
• GRAVY: -0.393836

⊟Function

• ⊞⊞TIGRFAM:
  Genetic information processing Protein synthesis Translation factors ribosome small subunit-dependent GTPase A (TIGR00157; EC 3.6.-.-; HMM-score: 244.9)
  and 30 more
  Genetic information processing Protein synthesis Other ribosome biogenesis GTPase YqeH (TIGR03597; HMM-score: 22.2)
  Cellular processes Cellular processes Chemotaxis and motility flagellar biosynthesis protein FlhF (TIGR03499; HMM-score: 21.5)
  Genetic information processing Protein synthesis Other ribosome biogenesis GTP-binding protein YsxC (TIGR03598; HMM-score: 19.7)
  Unknown function General small GTP-binding protein domain (TIGR00231; HMM-score: 19.6)
  Metabolism Transport and binding proteins Amino acids, peptides and amines urea ABC transporter, ATP-binding protein UrtE (TIGR03410; HMM-score: 18.9)
  Cellular processes Cellular processes Toxin production and resistance putative bacteriocin export ABC transporter, lactococcin 972 group (TIGR03608; HMM-score: 18.4)
  Metabolism Transport and binding proteins Unknown substrate putative bacteriocin export ABC transporter, lactococcin 972 group (TIGR03608; HMM-score: 18.4)
  Metabolism Transport and binding proteins Cations and iron carrying compounds cobalt ABC transporter, ATP-binding protein (TIGR01166; HMM-score: 16.2)
  Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Other FeS assembly ATPase SufC (TIGR01978; HMM-score: 16)
  Metabolism Central intermediary metabolism Phosphorus compounds phosphonate C-P lyase system protein PhnK (TIGR02323; HMM-score: 15.6)
  phosphonate C-P lyase system protein PhnL (TIGR02324; HMM-score: 15)
  Metabolism Transport and binding proteins Unknown substrate energy-coupling factor transporter ATPase (TIGR04521; EC 3.6.3.-; HMM-score: 15)
  Metabolism Transport and binding proteins Other thiamine ABC transporter, ATP-binding protein (TIGR01277; EC 3.6.3.-; HMM-score: 14.7)
  Genetic information processing Protein synthesis Other GTP-binding protein Era (TIGR00436; HMM-score: 14.2)
  Metabolism Transport and binding proteins Anions phosphate ABC transporter, ATP-binding protein (TIGR00972; EC 3.6.3.27; HMM-score: 13.8)
  Genetic information processing Protein fate Protein and peptide secretion and trafficking heme ABC exporter, ATP-binding protein CcmA (TIGR01189; EC 3.6.3.41; HMM-score: 13.6)
  Metabolism Transport and binding proteins Other heme ABC exporter, ATP-binding protein CcmA (TIGR01189; EC 3.6.3.41; HMM-score: 13.6)
  proposed F420-0 ABC transporter, ATP-binding protein (TIGR03873; HMM-score: 13.6)
  Metabolism Transport and binding proteins Anions sulfate ABC transporter, ATP-binding protein (TIGR00968; EC 3.6.3.25; HMM-score: 13.3)
  Metabolism Transport and binding proteins Amino acids, peptides and amines choline ABC transporter, ATP-binding protein (TIGR03415; HMM-score: 12.6)
  Genetic information processing Protein synthesis Other ribosome-associated GTPase EngA (TIGR03594; HMM-score: 12.6)
  Metabolism Transport and binding proteins Anions phosphonate ABC transporter, ATP-binding protein (TIGR02315; EC 3.6.3.28; HMM-score: 12.4)
  Metabolism Transport and binding proteins Other pleiotropic drug resistance family protein (TIGR00956; HMM-score: 12.2)
  Metabolism Transport and binding proteins Carbohydrates, organic alcohols, and acids ABC transporter, ATP-binding subunit, PQQ-dependent alcohol dehydrogenase system (TIGR03864; HMM-score: 12.1)
  thiol reductant ABC exporter, CydC subunit (TIGR02868; HMM-score: 12)
  Genetic information processing Protein synthesis tRNA and rRNA base modification tRNA 2-selenouridine synthase (TIGR03167; EC 2.9.1.-; HMM-score: 12)
  Metabolism Energy metabolism Amino acids and amines ethanolamine utilization protein, EutP (TIGR02528; HMM-score: 11.9)
  thiol reductant ABC exporter, CydD subunit (TIGR02857; HMM-score: 11.8)
  Genetic information processing Protein synthesis Other ribosome biogenesis GTP-binding protein YlqF (TIGR03596; HMM-score: 11.1)
  Metabolism Transport and binding proteins Other pigment precursor permease (TIGR00955; HMM-score: 10.6)
• TheSEED: see SPAP_1994
• ⊞⊞PFAM:
  P-loop_NTPase (CL0023) RsgA_GTPase; RsgA GTPase (PF03193; HMM-score: 195.5)
  and 16 more
  OB (CL0021) RsgA_N; RsgA N-terminal domain (PF16745; HMM-score: 88.6)
  P-loop_NTPase (CL0023) MMR_HSR1; 50S ribosome-binding GTPase (PF01926; HMM-score: 30.5)
  ABC_tran; ABC transporter (PF00005; HMM-score: 26.6)
  GTP_EFTU; Elongation factor Tu GTP binding domain (PF00009; HMM-score: 16.7)
  AAA_29; P-loop containing region of AAA domain (PF13555; HMM-score: 15.9)
  AAA_23; AAA domain (PF13476; HMM-score: 15.5)
  RNA_helicase; RNA helicase (PF00910; HMM-score: 15.1)
  AAA_22; AAA domain (PF13401; HMM-score: 15)
  NACHT; NACHT domain (PF05729; HMM-score: 14.5)
  AAA; ATPase family associated with various cellular activities (AAA) (PF00004; HMM-score: 14.4)
  AAA_16; AAA ATPase domain (PF13191; HMM-score: 13.6)
  FeoB_N; Ferrous iron transport protein B (PF02421; HMM-score: 13.1)
  AAA_25; AAA domain (PF13481; HMM-score: 12.1)
  NTPase_1; NTPase (PF03266; HMM-score: 11.9)
  NTPase_P4; ATPase P4 of dsRNA bacteriophage phi-12 (PF11602; HMM-score: 11.7)
  AIG1; AIG1 family (PF04548; HMM-score: 11.3)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• ⊞⊞PSORTb: Cytoplasmic
  • Cytoplasmic Score: 9.97
  • Cytoplasmic Membrane Score: 0
  • Cellwall Score: 0.01
  • Extracellular Score: 0.02
  • Internal Helices: 0
• ⊞⊞SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.022301
  • TAT(Tat/SPI): 0.001721
  • LIPO(Sec/SPII): 0.003898
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI:
• RefSeq: WP_001161981 NCBI
• UniProt:

⊟Protein sequence

• MQGQIIKALAGFYYVESDGQVYQTRARGNFRKKGHTPYVGDWVDFSAEENSEGYILKIHERKNSLVRPPIVNIDQAVVIMSVKEPDFNSNLLDRFLVLLEHKGIHPIVYISKMDLLEDRGELDFYQQTYGDIGYDFVTSKEELLSLLTGKVTVFMGQTGVGKSTLLNKIAPDLNLETGEISDSLGRGRHTTRAVSFYNLNGGKIADTPGFSSLDYEVSRAEDLNQAFPEIATVSRDCKFRTCTHTHEPSCAVKPAVEEGVIATFRFDNYLQFLSEIENRRETYKKVSKKIPK

⊟Experimental data

• protein localization:
• interaction partners:

⊟Expression & Regulation

⊟Operon

⊟Regulation

• regulator:

⊟Expression data

• PneumoExpress for strain D39V: SPV_1781 PneumoExpress

⊞Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊞Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications