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From PneumoWiki
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PangenomeTIGR4
serotype 4
D39
serotype 2
D39V
serotype 2
Hungary19A-6
serotype 19A
EF3030
serotype 19F
670-6B
serotype 6B
6A-10
serotype 6A
70585
serotype 5
A026
serotype 19F
A66
serotype 3
AP200
serotype 11A
ASP0581
serotype 12F
ATCC 49619
serotype 19F
ATCC 700669
serotype 23F
BM6001
serotype 19F
BVJ1JL
serotype 1
CGSP14
serotype 14
G54
serotype 19F
HU-OH
serotype 3
Hu15
serotype 19A
Hu17
serotype 19A
INV104
serotype 1
INV200
serotype 14
JJA
serotype 14
MDRSPN001
serotype 19F
NCTC7465
serotype 1
NCTC7466
serotype 2
NU83127
serotype 4
OXC141
serotype 3
P1031
serotype 1
R6
serotype 2
SP49
serotype 19A
SPN032672
serotype 1
SPN034156
serotype 3
SPN034183
serotype 3
SPN994038
serotype 3
SPN994039
serotype 3
SPNA45
serotype 3
ST556
serotype 19F
TCH8431/19A
serotype 19A
Taiwan19F-14
serotype 19F
Xen35
serotype 4
gamPNI0373
serotype 1

NCBI: 30-NOV-2024

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⊟Summary[edit | edit source]

Contents

  • 1 Summary
  • 2 Genome View
  • 3 Gene
    • 3.1 General
    • 3.2 Accession numbers
    • 3.3 Phenotype
    • 3.4 DNA sequence
  • 4 Protein
    • 4.1 General
    • 4.2 Function
    • 4.3 Structure, modifications & cofactors
    • 4.4 Localization
    • 4.5 Accession numbers
    • 4.6 Protein sequence
    • 4.7 Experimental data
  • 5 Expression & Regulation
    • 5.1 Operon
    • 5.2 Regulation
    • 5.3 Expression data
  • 6 Biological Material
    • 6.1 Mutants
    • 6.2 Expression vector
    • 6.3 lacZ fusion
    • 6.4 GFP fusion
    • 6.5 two-hybrid system
    • 6.6 FLAG-tag construct
    • 6.7 Antibody
  • 7 Other Information
  • 8 Literature
    • 8.1 References
    • 8.2 Relevant publications
  • organism: Streptococcus pneumoniae EF3030
  • locus tag: EF3030_RS10010 [old locus tag: EF3030_10010 ]
  • pan locus tag?: PNEUPAN003678000
  • symbol: EF3030_RS10010
  • pan gene symbol?: —
  • synonym:
  • product: class I SAM-dependent methyltransferase

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: EF3030_RS10010 [old locus tag: EF3030_10010 ]
  • symbol: EF3030_RS10010
  • product: class I SAM-dependent methyltransferase
  • replicon: chromosome
  • strand: -
  • coordinates: 1887224..1888177
  • length: 954
  • essential: unknown

⊟Accession numbers[edit | edit source]

  • Location: NZ_CP035897 (1887224..1888177) NCBI
  • BioCyc: EF3030_RS10010 BioCyc
  • MicrobesOnline:
  • PneumoBrowse for strain D39V: SPV_1854 PneumoBrowse

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    ATGGATTTTGAAAAAATTGAACAAGCTTATATCTATTTACTAGAGAATGTCCAAGTCATC
    CAAAGTGATTTGGCGACCAACTTTTATGACGCCTTGGTGGAGCAAAATAGCATCTATCTG
    GATGGTGAAACTGAGCTAAACCAGGTCAAAGACAACAATCAGGCCCTTAAGCGTTTAGCA
    CTACGCAAAGAAGAATGGCTCAAGACCTACCAGTTTCTCTTGATGAAGGCTGGGCAAACA
    GAACCCTTGCAGGCCAATCACCAGTTTACGCCAGATGCTATTGCCTTACTTTTGGTATTT
    ATTGTGGAAGAGTTGTTTACAGAGGAGGAAATTACTATCCTCGAAATGGGTTCTGGGATG
    GGAATTCTAGGCGCTACTTTCTTGACCTCGCTTGATAAAAAGGTGGATTACTTGGGAATG
    GAAGTGGATGATTTGCTGATTGATCTGGCAGCTAGCATGGCAGATGTAATTGGTTTGCAG
    GCTGGCTTTGTCCAAGGAGATGCCGTTCGCCCACAAATGCTCAAAGAAAGCGATGTGGTC
    ATCAGTGACTTGCCTGTTGGCTATTATCCTGATGATGCCGTTGCGTCGCGCCATCAAGTT
    GCTTCTAGCCAAGAACATACTTACGCTCATCACTTGCTCATGGAACAAGGGTTTAAGTAC
    CTTAAGTCAGATGGATATGCTATTTTTCTAGCTCCGAGTGATTTGTTGACCAGTCCTCAA
    AGTGATTTGTTGAAAGTCTGGTTGAAAGAGGAGGCAAGTCTGGTTGCTATGATTAGTTTA
    CCTGAAAATCTCTTTGCTAATGCTAAACAATCTAAGACTATTTTTATCCTACAGAAGAAA
    AGTGAAATAGCAGTGGAGCCTTTTGTTTATCCACTTGCTAGCTTGCAAGATGCAAGTGTT
    TTAATGAAATTTAAAGAAAATTTTCAAAAATGGACTCAAGGTACTGAAATATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    954

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: EF3030_RS10010 [old locus tag: EF3030_10010 ]
  • symbol: EF3030_RS10010
  • description: class I SAM-dependent methyltransferase
  • length: 317
  • theoretical pI: 4.22699
  • theoretical MW: 35676.6
  • GRAVY: -0.0201893

⊟Function[edit | edit source]

  • ⊞TIGRFAM:
    Genetic information processing Protein fate Protein modification and repair protein-(glutamine-N5) methyltransferase, release factor-specific (TIGR03534; EC 2.1.1.-; HMM-score: 18.7)
    Genetic information processing Protein synthesis tRNA and rRNA base modification ribosomal RNA small subunit methyltransferase A (TIGR00755; EC 2.1.1.182; HMM-score: 17.7)
    Genetic information processing Protein synthesis tRNA and rRNA base modification tRNA (guanine-N(7)-)-methyltransferase (TIGR00091; EC 2.1.1.33; HMM-score: 17.2)
    Genetic information processing DNA metabolism Restriction/modification type II restriction m6 adenine DNA methyltransferase, Alw26I/Eco31I/Esp3I family (TIGR02987; EC 2.1.1.72; HMM-score: 16.7)
    and 3 more
    Cell structure Cell envelope Biosynthesis and degradation of surface polysaccharides and lipopolysaccharides putative sugar O-methyltransferase (TIGR04371; EC 2.1.1.-; HMM-score: 13.8)
    Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Biotin malonyl-acyl carrier protein O-methyltransferase BioC (TIGR02072; EC 2.1.1.-; HMM-score: 13.4)
    Genetic information processing DNA metabolism Restriction/modification type I restriction-modification system, M subunit (TIGR00497; EC 2.1.1.72; HMM-score: 11.7)
  • TheSEED: data available for D39, Hungary19A-6, TIGR4
  • ⊞PFAM:
    NADP_Rossmann (CL0063) N6_Mtase; N-6 DNA Methylase (PF02384; HMM-score: 33.2)
    and 6 more
    MTS; Methyltransferase small domain (PF05175; HMM-score: 18.8)
    Methyltransf_25; Methyltransferase domain (PF13649; HMM-score: 18.4)
    Methyltransf_31; Methyltransferase domain (PF13847; HMM-score: 17.2)
    Methyltransf_32; Methyltransferase domain (PF13679; HMM-score: 12.5)
    RrnaAD; Ribosomal RNA adenine dimethylase (PF00398; HMM-score: 12.3)
    PCMT; Protein-L-isoaspartate(D-aspartate) O-methyltransferase (PCMT) (PF01135; HMM-score: 12.3)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • ⊞PSORTb: Cytoplasmic Membrane
    • Cytoplasmic Score: 0.32
    • Cytoplasmic Membrane Score: 9.55
    • Cellwall Score: 0.12
    • Extracellular Score: 0.01
    • Internal Helices: 0
  • ⊞SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.003481
    • TAT(Tat/SPI): 0.000114
    • LIPO(Sec/SPII): 0.000434
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

  • GI:
  • RefSeq: WP_000345086 NCBI
  • UniProt:

⊟Protein sequence[edit | edit source]

  • MDFEKIEQAYIYLLENVQVIQSDLATNFYDALVEQNSIYLDGETELNQVKDNNQALKRLALRKEEWLKTYQFLLMKAGQTEPLQANHQFTPDAIALLLVFIVEELFTEEEITILEMGSGMGILGATFLTSLDKKVDYLGMEVDDLLIDLAASMADVIGLQAGFVQGDAVRPQMLKESDVVISDLPVGYYPDDAVASRHQVASSQEHTYAHHLLMEQGFKYLKSDGYAIFLAPSDLLTSPQSDLLKVWLKEEASLVAMISLPENLFANAKQSKTIFILQKKSEIAVEPFVYPLASLQDASVLMKFKENFQKWTQGTEI

⊟Experimental data[edit | edit source]

  • protein localization:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator:

⊟Expression data[edit | edit source]

  • PneumoExpress for strain D39V: SPV_1854 PneumoExpress

⊞Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊞Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

⊟Relevant publications[edit | edit source]

Retrieved from "http://pneumowiki.med.uni-greifswald.de/index.php?title=EF3030_RS10010&oldid=15262"
  • This page was last edited on 20 January 2022, at 13:38.
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