⊟Summary[edit | edit source]

PangenomeTIGR4

serotype 4

D39

serotype 2

D39V

serotype 2

Hungary19A-6

serotype 19A

EF3030

serotype 19F

670-6B

serotype 6B

6A-10

serotype 6A

70585

serotype 5

A026

serotype 19F

A66

serotype 3

AP200

serotype 11A

ASP0581

serotype 12F

ATCC 49619

serotype 19F

ATCC 700669

serotype 23F

BVJ1JL

serotype 1

CGSP14

serotype 14

G54

serotype 19F

HU-OH

serotype 3

Hu15

serotype 19A

Hu17

serotype 19A

INV104

serotype 1

INV200

serotype 14

JJA

serotype 14

MDRSPN001

serotype 19F

NCTC7466

serotype 2

NU83127

serotype 4

OXC141

serotype 3

P1031

serotype 1

R6

serotype 2

SP49

serotype 19A

SPN032672

serotype 1

SPN034156

serotype 3

SPN034183

serotype 3

SPN994038

serotype 3

SPN994039

serotype 3

SPNA45

serotype 3

ST556

serotype 19F

TCH8431/19A

serotype 19A

Taiwan19F-14

serotype 19F

Xen35

serotype 4

gamPNI0373

serotype 1

NCBI: 20-DEC-2020

⊟Summary

• organism: Streptococcus pneumoniae OXC141
locus tag: SPNOXC_RS02530 [old locus tags: SPNOXC04660 SPNOXC_04660 ]
pan locus tag^?: PNEUPAN001387000
symbol: glnA
pan gene symbol^?: glnA
• synonym:
• product: type I glutamate--ammonia ligase

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: SPNOXC_RS02530 [old locus tags: SPNOXC04660 SPNOXC_04660 ]
• symbol: glnA
• product: type I glutamate--ammonia ligase
• replicon: chromosome
• strand: +
• coordinates: 468070..469416
• length: 1347
• essential: unknown

⊟Accession numbers

• Location: NC_017592 (468070..469416) NCBI
• BioCyc: SPNOXC_RS02530 BioCyc
• MicrobesOnline:
• PneumoBrowse for strain D39V: SPV_0448 PneumoBrowse

⊟Phenotype

Share your knowledge and add information here. [edit]

⊟DNA sequence

• 1
  61
  121
  181
  241
  301
  361
  421
  481
  541
  601
  661
  721
  781
  841
  901
  961
  1021
  1081
  1141
  1201
  1261
  1321
  
  ATGCCAATCACAGCTGCAGATATTCGTCGTGAAGTCAAGGAAAAAAATGTTACCTTTATT
  CGTCTTATGTTCTCAGATATTTTGGGAACCATGAAAAACGTCGAAATTCCTGCTACAGAT
  GAACAGTTAGATAAGGTCTTGTCGAACAAGGTTATGTTTGATGGATCTTCTATTGAAGGT
  TTTGTACGTATCAATGAGTCGGATATGTACTTGTACCCGGACTTGGATACATGGACAGTC
  TTCCCTTGGGGAGATGAAAATGGAAGTGTTGCAGGTCTGATCTGTGATGTCTATACAACA
  GAAGGTGAACCATTTGCGGGTGACCCTCGTGGTAATTTGAAACGAGCTCTTCGTCACATG
  GAAGAAGTTGGATTCAAATCCTTCAATCTTGGTCCAGAGCCAGAATTCTTCCTATTTAAG
  TTGGATGAAAATGGGGACCCAACACTTGAAGTGAATGACAAGGGTGGCTACTTTGATTTG
  GCACCTACTGACCTTGCGGACAACACACGTCGTGAGATTGTGAATGTCTTGACCAAAATG
  GGATTTGAAGTAGAAGCGAGTCACCACGAGGTTGCGGTTGGACAGCATGAGATTGACTTT
  AAGTACGATGAAGTTCTCCGTGCTTGTGATAAGATTCAAATCTTTAAACTTGTTGTTAAA
  ACCATTGCTCGCAAACACGGACTTTACGCAACATTTATGGCGAAGCCAAAATTTGGTATT
  GCTGGATCAGGTATGCACTGTAATATGTCCTTGTTTGATGCAGAAGGAAATAACGCCTTC
  TTTGATCCAAATGATCCAAAAGGAATGCAGTTGTCAGAAACAGCTTACCATTTCCTAGGC
  GGTTTGATCAAGCATGCTTACAACTATACTGCCATCATGAACCCAACAGTTAACTCATAC
  AAACGTTTGGTTCCAGGTTATGAAGCGCCTGTTTACATTGCTTGGGCTGGTCGTAACCGT
  TCGCCACTTGTGCGCGTACCTGCTTCACGTGGTATGGGAACTCGTCTTGAGTTGCGTTCA
  GTGGATCCAATGGCGAACCCTTACATTGCTATGGCTGTTCTTTTGGAAGTTGGTTTGCAT
  GGTATTGAAAATAAAATCGAAGCACCAGCTCCTATCGAAGAAAATATCTACATCATGACA
  GCAGAAGAGCGCAAGGAAGCTGGTATTACAGACCTTCCATCAACTCTTCACAACGCTTTG
  AAAGCTTTGACAGAAGATGAAGTGGTTAAAGCTGCTCTCGGAGATCACATCTACACTAGC
  TTCCTTGAAGCCAAACGAATCGAATGGGCAAGTTATGCAACCTTCGTTTCACAATGGGAA
  ATTGATAATTATTTAGACCTTTACTAA
  60
  120
  180
  240
  300
  360
  420
  480
  540
  600
  660
  720
  780
  840
  900
  960
  1020
  1080
  1140
  1200
  1260
  1320
  1347
  

⊟Protein

⊟General

• locus tag: SPNOXC_RS02530 [old locus tags: SPNOXC04660 SPNOXC_04660 ]
• symbol: GlnA
• description: type I glutamate--ammonia ligase
• length: 448
• theoretical pI: 4.72245
• theoretical MW: 50309.9
• GRAVY: -0.235714

⊟Function

• ⊞⊞reaction:
  EC 6.3.1.2?  ExPASy
  Glutamine synthetase ATP + L-glutamate + NH₃ = ADP + phosphate + L-glutamine
• ⊞⊞TIGRFAM:
  Metabolism Amino acid biosynthesis Glutamate family glutamine synthetase, type I (TIGR00653; EC 6.3.1.2; HMM-score: 560)
  and 1 more
  glutamine synthetase, type III (TIGR03105; EC 6.3.1.2; HMM-score: 363.2)
• TheSEED: see SPNOXC04660
• ⊞⊞PFAM:
  GCS (CL0286) Gln-synt_C; Glutamine synthetase, catalytic domain (PF00120; HMM-score: 456.2)
  and 1 more
  no clan defined Gln-synt_N; Glutamine synthetase, beta-Grasp domain (PF03951; HMM-score: 98.7)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• ⊞⊞PSORTb: Cytoplasmic
  • Cytoplasmic Score: 9.97
  • Cytoplasmic Membrane Score: 0
  • Cellwall Score: 0.01
  • Extracellular Score: 0.02
  • Internal Helices: 0
• ⊞⊞SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.003788
  • TAT(Tat/SPI): 0.001575
  • LIPO(Sec/SPII): 0.000782
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI:
• RefSeq: WP_001122899 NCBI
• UniProt:

⊟Protein sequence

• MPITAADIRREVKEKNVTFIRLMFSDILGTMKNVEIPATDEQLDKVLSNKVMFDGSSIEGFVRINESDMYLYPDLDTWTVFPWGDENGSVAGLICDVYTTEGEPFAGDPRGNLKRALRHMEEVGFKSFNLGPEPEFFLFKLDENGDPTLEVNDKGGYFDLAPTDLADNTRREIVNVLTKMGFEVEASHHEVAVGQHEIDFKYDEVLRACDKIQIFKLVVKTIARKHGLYATFMAKPKFGIAGSGMHCNMSLFDAEGNNAFFDPNDPKGMQLSETAYHFLGGLIKHAYNYTAIMNPTVNSYKRLVPGYEAPVYIAWAGRNRSPLVRVPASRGMGTRLELRSVDPMANPYIAMAVLLEVGLHGIENKIEAPAPIEENIYIMTAEERKEAGITDLPSTLHNALKALTEDEVVKAALGDHIYTSFLEAKRIEWASYATFVSQWEIDNYLDLY

⊟Experimental data

• protein localization:
• interaction partners:

⊟Expression & Regulation

⊟Operon

⊟Regulation

• data available for TIGR4

⊟Expression data

• PneumoExpress for strain D39V: SPV_0448 PneumoExpress

⊞Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊞Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications

Tomas G Kloosterman, Wouter T Hendriksen, Jetta J E Bijlsma, Hester J Bootsma, Sacha A F T van Hijum, Jan Kok, Peter W M Hermans, Oscar P Kuipers
Regulation of glutamine and glutamate metabolism by GlnR and GlnA in Streptococcus pneumoniae.
J Biol Chem: 2006, 281(35);25097-109
[PubMed:16787930] [WorldCat.org] [DOI] (P p)