⊟Summary[edit | edit source]

PangenomeTIGR4

serotype 4

D39

serotype 2

D39V

serotype 2

Hungary19A-6

serotype 19A

EF3030

serotype 19F

670-6B

serotype 6B

6A-10

serotype 6A

70585

serotype 5

A026

serotype 19F

A66

serotype 3

AP200

serotype 11A

ASP0581

serotype 12F

ATCC 49619

serotype 19F

ATCC 700669

serotype 23F

BVJ1JL

serotype 1

CGSP14

serotype 14

G54

serotype 19F

HU-OH

serotype 3

Hu15

serotype 19A

Hu17

serotype 19A

INV104

serotype 1

INV200

serotype 14

JJA

serotype 14

MDRSPN001

serotype 19F

NCTC7466

serotype 2

NU83127

serotype 4

OXC141

serotype 3

P1031

serotype 1

R6

serotype 2

SP49

serotype 19A

SPN032672

serotype 1

SPN034156

serotype 3

SPN034183

serotype 3

SPN994038

serotype 3

SPN994039

serotype 3

SPNA45

serotype 3

ST556

serotype 19F

TCH8431/19A

serotype 19A

Taiwan19F-14

serotype 19F

Xen35

serotype 4

gamPNI0373

serotype 1

NCBI: 07-FEB-2021

⊟Summary

• organism: Streptococcus pneumoniae AP200
locus tag: SPAP_RS05015 [old locus tag: SPAP_1010 ]
pan locus tag^?: PNEUPAN002004000
symbol: tehB
pan gene symbol^?: tehB
• synonym:
• product: SAM-dependent methyltransferase TehB

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: SPAP_RS05015 [old locus tag: SPAP_1010 ]
• symbol: tehB
• product: SAM-dependent methyltransferase TehB
• replicon: chromosome
• strand: +
• coordinates: 956542..957402
• length: 861
• essential: unknown

⊟Accession numbers

• Location: NC_014494 (956542..957402) NCBI
• BioCyc: SPAP_RS05015 BioCyc
• MicrobesOnline:
• PneumoBrowse for strain D39V: SPV_0864 PneumoBrowse

⊟Phenotype

Share your knowledge and add information here. [edit]

⊟DNA sequence

• 1
  61
  121
  181
  241
  301
  361
  421
  481
  541
  601
  661
  721
  781
  841
  
  ATGGAAAAATTAGTTGCCTATAAACGCATGCCTTTGTGGAATAAACAAACAATGCCTGAA
  GCTGTTCAGCAAAAGCACAATACAAAAGTTGGGACTTGGGGGAAAATTACTGTCTTGAAG
  GGAGCTCTCAAGTTTATTGAATTGACAGAAGAAGGGGAAGTTCTAGCTGAACACCTCTTT
  GAAGCAGGGGCAGACAATCCAATGGCCCAACCTCAAGCCTGGCACCGAGTGGAAGCTGCC
  ACAGATGATGTGGAATGGTACTTGGAATTTTATTGTAAACCTGAGGATTATTTTGCTAAA
  AAATACAATACCAATCCTGTTCATTCAGAGGTCCTAGAGGCCATGCAGACAGTGAAACAA
  GGGAAAGCTTTGGATTTAGGTTGTGGTCAGGGGCGTAATTCTCTTTTTCTAGCCCAGCAA
  GATTTTGATGTGACAGCTGTAGATCAAAATGGACTAGCTCTTGAAATCTTGCAAAGCATT
  GTGGAGCAGGAAGATTTGGACATGCCTGTTGGCCTTTACGATATCAATTCAGCTAGCATT
  GAACAAGAATATGATTTTATCGTTTCAACAGTTGTTCTCATGTTTCTACAAGCGGACCGC
  ATTCCAGCTATTATTCAAAATATGCAGGAGAAAACCAGTGTTGGTGGTTACAACCTTATC
  GTTTGTGCCATGGACACGGAGGATTATCCTTGCTCGGTTAACTTCCCATTCACCTTTAAA
  GAAGGAGAACTGGCAGACTATTACAAGGATTGGGAATTGGTTAAGTACAATGAAAATCCA
  GGCCATTTGCATCGTCGCGATGAGAATGGCAATCGTATTCAACTACGCTTTGCGACCTTA
  CTAGCTAAGAAAATCAAGTAA
  60
  120
  180
  240
  300
  360
  420
  480
  540
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  720
  780
  840
  861
  

⊟Protein

⊟General

• locus tag: SPAP_RS05015 [old locus tag: SPAP_1010 ]
• symbol: TehB
• description: SAM-dependent methyltransferase TehB
• length: 286
• theoretical pI: 4.55746
• theoretical MW: 32684.9
• GRAVY: -0.39021

⊟Function

• reaction:
  EC 2.1.1.-?  ExPASy
• ⊞⊞TIGRFAM:
  Cellular processes Cellular processes Toxin production and resistance tellurite resistance protein TehB (TIGR00477; HMM-score: 360.5)
  and 12 more
  Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Chlorophyll and bacteriochlorphyll magnesium protoporphyrin O-methyltransferase (TIGR02021; EC 2.1.1.11; HMM-score: 28.6)
  Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Glutathione and analogs putative 4-mercaptohistidine N1-methyltranferase (TIGR04345; HMM-score: 26.5)
  Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Menaquinone and ubiquinone 3-demethylubiquinone-9 3-O-methyltransferase (TIGR01983; EC 2.1.1.64; HMM-score: 25.6)
  Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Biotin malonyl-acyl carrier protein O-methyltransferase BioC (TIGR02072; EC 2.1.1.-; HMM-score: 24.9)
  Genetic information processing Protein fate Protein modification and repair protein-(glutamine-N5) methyltransferase, release factor-specific (TIGR03534; EC 2.1.1.-; HMM-score: 23.2)
  Genetic information processing Protein synthesis Ribosomal proteins: synthesis and modification ribosomal protein L11 methyltransferase (TIGR00406; EC 2.1.1.-; HMM-score: 17.8)
  Genetic information processing Protein fate Protein modification and repair methyltransferase, HemK family (TIGR00536; HMM-score: 16.2)
  pseudaminic acid biosynthesis-associated methylase (TIGR03587; HMM-score: 15.3)
  Genetic information processing Protein synthesis tRNA and rRNA base modification 23S rRNA (uracil-5-)-methyltransferase RumA (TIGR00479; EC 2.1.1.-; HMM-score: 14.4)
  cupin fold metalloprotein, WbuC family (TIGR04366; HMM-score: 14)
  Genetic information processing Transcription RNA processing 3' terminal RNA ribose 2'-O-methyltransferase Hen1 (TIGR04074; EC 2.1.1.-; HMM-score: 11.1)
  Genetic information processing Protein synthesis tRNA and rRNA base modification 3' terminal RNA ribose 2'-O-methyltransferase Hen1 (TIGR04074; EC 2.1.1.-; HMM-score: 11.1)
• TheSEED: see SPAP_1010
• ⊞⊞PFAM:
  NADP_Rossmann (CL0063) TehB; Tellurite resistance protein TehB (PF03848; HMM-score: 308)
  and 14 more
  Cupin (CL0029) DUF1971; Domain of unknown function (DUF1971) (PF09313; HMM-score: 97.6)
  NADP_Rossmann (CL0063) Methyltransf_25; Methyltransferase domain (PF13649; HMM-score: 40.7)
  Methyltransf_23; Methyltransferase domain (PF13489; HMM-score: 37.7)
  MTS; Methyltransferase small domain (PF05175; HMM-score: 37.2)
  Methyltransf_31; Methyltransferase domain (PF13847; HMM-score: 35.8)
  Methyltransf_11; Methyltransferase domain (PF08241; HMM-score: 30.3)
  Methyltransf_12; Methyltransferase domain (PF08242; HMM-score: 25)
  NodS; Nodulation protein S (NodS) (PF05401; HMM-score: 20)
  CMAS; Mycolic acid cyclopropane synthetase (PF02353; HMM-score: 17.9)
  no clan defined TRIC; TRIC channel (PF05197; HMM-score: 17.3)
  NADP_Rossmann (CL0063) TPMT; Thiopurine S-methyltransferase (TPMT) (PF05724; HMM-score: 13.4)
  PRMT5; PRMT5 arginine-N-methyltransferase (PF05185; HMM-score: 12.7)
  Methyltransf_8; Hypothetical methyltransferase (PF05148; HMM-score: 12.4)
  CheR; CheR methyltransferase, SAM binding domain (PF01739; HMM-score: 11.9)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• ⊞⊞PSORTb: Cytoplasmic
  • Cytoplasmic Score: 7.5
  • Cytoplasmic Membrane Score: 1.15
  • Cellwall Score: 0.62
  • Extracellular Score: 0.73
  • Internal Helices: 0
• ⊞⊞SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.00556
  • TAT(Tat/SPI): 0.000203
  • LIPO(Sec/SPII): 0.000368
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI:
• RefSeq: WP_000413762 NCBI
• UniProt:

⊟Protein sequence

• MEKLVAYKRMPLWNKQTMPEAVQQKHNTKVGTWGKITVLKGALKFIELTEEGEVLAEHLFEAGADNPMAQPQAWHRVEAATDDVEWYLEFYCKPEDYFAKKYNTNPVHSEVLEAMQTVKQGKALDLGCGQGRNSLFLAQQDFDVTAVDQNGLALEILQSIVEQEDLDMPVGLYDINSASIEQEYDFIVSTVVLMFLQADRIPAIIQNMQEKTSVGGYNLIVCAMDTEDYPCSVNFPFTFKEGELADYYKDWELVKYNENPGHLHRRDENGNRIQLRFATLLAKKIK

⊟Experimental data

• protein localization:
• interaction partners:

⊟Expression & Regulation

⊟Operon

⊟Regulation

• regulator:

⊟Expression data

• PneumoExpress for strain D39V: SPV_0864 PneumoExpress

⊞Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊞Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications